Purification and separation of caffeoyl spermidine derivatives from goji leaf tea with ion exchange resin and the mechanisms involved

IF 3.5 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Food and Bioproducts Processing Pub Date : 2024-06-11 DOI:10.1016/j.fbp.2024.06.005
Weibiao Wang , Yuping Sa , Weiman Zhang , Xiaoying Wang , Yinli Wang , Hui Yuan , Liuyan Li , Shuqin Ding , Gidion Wilson , Xueqin Ma
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Abstract

Goji leaf (Lycium barbarum leaves, LBL) tea is a well-known beverage that has been developed and utilized for its numerous health benefits. Recently, we have successfully extracted four caffeoyl spermidines derivatives from LBL (LBLS), namely N-caffeoylputrescine, N-acetyl-N′-caffeoylputrescine, N1-dihydrocaffeoyl-N10-caffeoylspermidine and N1, N10-dicaffeoylspermidine. Given the diverse bioactivities exhibited by LBLS, our study aimed to develop a precise separation method and explore the possible purification mechanism. Firstly, the extraction process was optimized, followed by the selection of 001×7 resin for the enrichment and purification of LBLS from six resins. Subsequently, the adsorption mechanism was comprehensively examined using FT-IR, DSC, XRD, and XPS techniques. Additionally, investigations into the adsorption kinetics, isotherm models, and adsorption thermodynamics revealed the adsorption process of LBLS on 001×7 resin was spontaneous and exothermic, followed a monolayer adsorption mechanism, and conformed to the pseudo-second-order kinetic model and Langmuir model. The optimal procedure involved adsorbing a 25 mg/mL LBL extract onto a 3.5 BV (bed volume) at 2 BV/h, then eluting with an 8 % NaCl-55 % ethanol solution for 5 BV at the same rate. Finally, LBLS was separated using PHPLC to obtain monomer compounds. This process yields 1.57 %±0.1 % LBLS, and four monomers with purity range from 90.7 % to 100 % were obtained.

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用离子交换树脂纯化和分离枸杞叶茶中的咖啡酰亚精胺衍生物及其作用机理
枸杞叶茶(Lycium barbarum leaves, LBL)是一种众所周知的饮品,因其具有众多保健功效而被开发和利用。最近,我们成功地从枸杞叶中提取了四种咖啡酰精胺衍生物,即 N-咖啡酰枸杞碱、N-乙酰基-N′-咖啡酰枸杞碱、N1-二氢咖啡酰-N10-咖啡酰枸杞碱和 N1,N10-二咖啡酰枸杞碱。鉴于枸杞多糖具有多种生物活性,我们的研究旨在开发一种精确的分离方法并探索可能的纯化机制。首先,对提取工艺进行了优化,然后从六种树脂中选择了 001×7 树脂对枸杞多糖进行富集和纯化。随后,利用 FT-IR、DSC、XRD 和 XPS 技术对吸附机理进行了全面研究。此外,对吸附动力学、等温线模型和吸附热力学的研究表明,枸杞多糖在 001×7 树脂上的吸附过程是自发的、放热的,遵循单层吸附机理,符合伪二阶动力学模型和 Langmuir 模型。最佳程序是在 3.5 BV(床体积)上以 2 BV/h 的速度吸附 25 mg/mL 的枸杞多糖提取物,然后以相同的速度用 8 % NaCl-55 % 乙醇溶液洗脱 5 BV。最后,使用 PHPLC 分离 LBLS,获得单体化合物。此过程可获得 1.57 %±0.1 % 的枸杞多糖,并得到纯度范围为 90.7 % 至 100 % 的四种单体。
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来源期刊
Food and Bioproducts Processing
Food and Bioproducts Processing 工程技术-工程:化工
CiteScore
9.70
自引率
4.30%
发文量
115
审稿时长
24 days
期刊介绍: Official Journal of the European Federation of Chemical Engineering: Part C FBP aims to be the principal international journal for publication of high quality, original papers in the branches of engineering and science dedicated to the safe processing of biological products. It is the only journal to exploit the synergy between biotechnology, bioprocessing and food engineering. Papers showing how research results can be used in engineering design, and accounts of experimental or theoretical research work bringing new perspectives to established principles, highlighting unsolved problems or indicating directions for future research, are particularly welcome. Contributions that deal with new developments in equipment or processes and that can be given quantitative expression are encouraged. The journal is especially interested in papers that extend the boundaries of food and bioproducts processing. The journal has a strong emphasis on the interface between engineering and food or bioproducts. Papers that are not likely to be published are those: • Primarily concerned with food formulation • That use experimental design techniques to obtain response surfaces but gain little insight from them • That are empirical and ignore established mechanistic models, e.g., empirical drying curves • That are primarily concerned about sensory evaluation and colour • Concern the extraction, encapsulation and/or antioxidant activity of a specific biological material without providing insight that could be applied to a similar but different material, • Containing only chemical analyses of biological materials.
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