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Framework for scaling-up extraction processes in nutraceutical beverages: A simulation, techno-economic, and environmental analysis approach 扩大营养保健饮品提取工艺的框架:模拟、技术经济和环境分析方法
IF 3.5 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-09-01 DOI: 10.1016/j.fbp.2024.08.010

The nutraceutical beverages market has increased in recent years, motivated by the increasing trend of consumers choosing food and beverages beneficial to health, mostly after the COVID-19 pandemic. Several researchers have proposed different formulations, where the combination of plants has been tested at the laboratory and pilot scales to maximize the desirable features of the beverages, including antioxidant capacity, anticarcinogens, and anti-inflammatory properties. Developing these products requires scaling-up from these scales to the industry one and, hence, identifying the criteria and/or parameters affecting process yield due to the transport phenomena associated with the scale increment. This work proposes a framework for scaling up solid-liquid extraction in a nutraceutical beverage process using available pilot plant data, combining brute-force and empirical scaling approaches. This framework provides an alternative for industries that have acquired equipment without considering the principles of similarity between the larger scale and the laboratory stage. Operating conditions are tuned to reach the product quality at the pilot level and the maximum beverage's antioxidant capacity. A techno-economic analysis of the production process and an environmental evaluation were performed, providing the basis for an effective scaling-up to the industry level. The scaling-up proved to be feasible, as the net present value of the process is $2018,000 with a payback time of 4.83 years; the major source of solid waste is the raw materials with a carbon footprint less than 0.205 MT eCO2 due this process operates with temperatures lower than 100 °C. The circular economy indicators in this project were circular material usage rate and Waste Stream Recycling Rate. The Circular Material Usage Rate ranged from 16.7 % to 66.7 % depending on the composition of the cocoa husk in the raw material, and the Waste Stream Recycling Rate (%) ranged from 4.4 % to 5 % destined for composting development. The framework is designed to be applicable to other food production processes that encounter equipment constraints. It facilitates the evaluation of process yield and enables the simulation and analysis of economic profitability and environmental impact using circular economy indicators at an industrial/commercial scale.

近年来,消费者越来越倾向于选择有益于健康的食品和饮料,主要是在 COVID-19 大流行之后,营养保健饮料市场也随之增长。一些研究人员提出了不同的配方,并在实验室和试验规模上对植物组合进行了测试,以最大限度地提高饮料的理想特性,包括抗氧化能力、抗癌和抗炎特性。开发这些产品需要从这些规模扩大到工业规模,因此需要确定影响工艺产量的标准和/或参数,这些标准和/或参数是与规模扩大相关的传输现象造成的。这项工作提出了一个框架,利用现有的中试工厂数据,结合蛮力和经验缩放方法,对营养保健饮料工艺中的固液萃取进行缩放。该框架为那些已购置设备但未考虑大规模和实验室阶段相似性原则的行业提供了一种替代方案。对操作条件进行了调整,以达到中试水平的产品质量和饮料的最大抗氧化能力。对生产过程进行了技术经济分析和环境评估,为有效地将规模扩大到工业水平奠定了基础。事实证明,扩大规模是可行的,因为该工艺的净现值为 2018,000 美元,投资回收期为 4.83 年;固体废物的主要来源是原材料,由于该工艺的操作温度低于 100 °C,其碳足迹小于 0.205 MT eCO2。该项目的循环经济指标是循环材料使用率和废物流回收率。根据原材料中可可壳的成分,循环材料使用率从 16.7% 到 66.7% 不等;废物流回收率(%)从 4.4% 到 5% 不等,主要用于堆肥发展。该框架适用于遇到设备限制的其他食品生产过程。它有助于评估工艺产量,并能在工业/商业规模上使用循环经济指标模拟和分析经济盈利能力和环境影响。
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引用次数: 0
Impact of pre-treatments and bioprocessing on the carbohydrate and polyphenol profile of brewers’ spent grain 预处理和生物加工对酿酒废谷碳水化合物和多酚特征的影响
IF 3.5 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-08-30 DOI: 10.1016/j.fbp.2024.08.017

Brewers’ spent grain (BSG), the solid waste of the brewing industry, is high in fibres, proteins and health-beneficial compounds such as polyphenols. This research investigated bioprocessing with enzymes and microbes to modify the properties of BSG for its utilisation as a food ingredient. Pre-treatment studies showed that wet milling performed better than dry milling, and heat and homogenisation either before or after the enzyme hydrolysis did not significantly influence the release of reducing sugars and free amino nitrogen (FAN). Four treatments were applied to wet-milled BSG: fermentation with Lactiplantibacillus plantarum POM1 with or without the enzyme Ondea pro and enzymatic bioprocessing without any fermentation. Control was the condition without enzyme and starter. Without the enzyme, there was negligible free sugar and FAN, and the starter had limited growth and organic acid production. Only the combination of enzymatic hydrolysis and fermentation reached a pH of 4 and 10 mg/g DW lactic acid. The microbial preference for monosaccharides was evident, and the enzyme influenced the release of oligosaccharides that can have a prebiotic effect. Bioprocessing impacted the phenolic acid composition and microbial consumption, with a significant release of ferulic acid during enzyme hydrolysis.

酿酒师用过的谷物(BSG)是酿酒业的固体废物,含有大量纤维、蛋白质和有益健康的化合物(如多酚)。这项研究调查了用酶和微生物进行生物处理以改变 BSG 的特性,从而将其用作食品配料的情况。预处理研究表明,湿法研磨比干法研磨效果更好,酶水解前后的加热和均质化对还原糖和游离氨基酸氮(FAN)的释放没有显著影响。对湿磨 BSG 采用了四种处理方法:用植物乳杆菌 POM1 与或不与 Ondea pro 酶一起发酵,以及不进行任何发酵的酶生物处理。对照组是不加酶和启动剂的条件。在不加酶的情况下,游离糖和 FAN 的含量微乎其微,启动子的生长和有机酸的产生也很有限。只有酶水解和发酵相结合才能达到 pH 值 4 和 10 mg/g DW 乳酸。微生物对单糖的偏好是显而易见的,酶影响了具有益生作用的低聚糖的释放。生物处理对酚酸组成和微生物消耗量有影响,在酶水解过程中会释放出大量阿魏酸。
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引用次数: 0
Efficient isolation of rhamnolipids from fermentation broth via an advanced acid-induced precipitation process with heat treatment 通过先进的酸诱导沉淀加热处理工艺从发酵液中高效分离鼠李糖脂
IF 3.5 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-08-30 DOI: 10.1016/j.fbp.2024.08.016

Acid-induced precipitation (AIP) is the most efficient approach for isolation of rhamnolipids (RLs), while it does not work at certain cases. Therefore, for certain specific fermentation processes, efficient and low-cost separation of RLs remains a challenge. In this study, an advanced AIP process based on heat treatment was developed to efficiently isolate RLs from a fermentation broth where RLs were unable to precipitate. Over 97 % of RLs with purity of 90.8 % were recovered after treatment at 120 °C for 10 min at a pH below 4. The involved mechanism might be related to that heat treatment at pH below 4 results in the degradation of certain components in broth that interfere with the RLs precipitation, thus no longer affecting the precipitation performance of RLs at acidic conditions. Moreover, the obtained RLs manifested well-maintained surface and emulsification activities. It can reduce the surface tension of water to approximately 29 mN/m at concentration of 65 mg/L, and the emulsification index was 72–80 % against varied types of oil. The approach exhibited superior performance compared to regular AIP processes, demonstrating excellent universality in real applications. However, more systematic research needs to focus on clarifying the interfering component which should be the main limitation of the present study.

酸诱导沉淀法(AIP)是分离鼠李糖脂(RLs)的最有效方法,但在某些情况下并不起作用。因此,在某些特定的发酵过程中,高效、低成本地分离鼠李糖脂仍是一项挑战。本研究开发了一种基于热处理的先进 AIP 工艺,可从 RL 无法沉淀的发酵液中高效分离 RL。在 pH 值低于 4 的条件下,经过 120 °C 10 分钟的处理后,超过 97% 的 RLs 被回收,纯度达 90.8%。其中的机理可能与 pH 值低于 4 的热处理会导致肉汤中干扰 RLs 沉淀的某些成分降解有关,从而不再影响 RLs 在酸性条件下的沉淀性能。此外,获得的 RLs 还具有良好的表面活性和乳化活性。当浓度为 65 毫克/升时,它能将水的表面张力降低到约 29 毫牛顿/米,对各种油类的乳化指数为 72-80%。与普通的 AIP 工艺相比,该方法表现出更优越的性能,在实际应用中具有极佳的通用性。不过,更系统化的研究需要重点明确干扰成分,这应该是本研究的主要局限。
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引用次数: 0
Carbon footprint analysis of sugar production in Poland 波兰蔗糖生产的碳足迹分析
IF 3.5 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-08-29 DOI: 10.1016/j.fbp.2024.08.014

Food production is a major contributor to greenhouse gas emissions and biodiversity loss, highlighting the need for a comprehensive approach to identify and reduce emissions. Efficient energy use is critical, alongside the adoption of low-carbon technologies that help agriculture and food processing adapt to climate change. Carbon footprint (CF) analysis is a key tool for assessing the environmental impact of food production and distribution, requiring a thorough evaluation of each product's life cycle from production to consumption. This study focused on the sugar production CF in three Polish plants, examining technological processes and creating unit process diagrams of the production cycle. This analysis led to the development of a database to calculate the CF based on production volume. The determined CF was 0.14–0.27 kg CO2eq/kg, and the average CFav: for plant 1–0.18; for plant 2–0.19; for plant 3–0.19 kg CO2eq/kg. Continuous monitoring is essential, allowing production practices to adapt to changing conditions and ensuring quick responses to sustainability needs. Reducing the sugar production CF involves several strategies, including adopting sustainable cultivation practices, optimizing production processes, using renewable energy sources, improving transportation efficiency, and minimizing waste. Together, these measures promote more environmentally responsible sugar production. By prioritizing sustainability and embracing innovative solutions, the food industry can significantly reduce its environmental impact, meeting the challenges of climate change and biodiversity loss.

粮食生产是温室气体排放和生物多样性丧失的主要因素,因此需要采取综合办法来确定和减少排放。高效利用能源以及采用有助于农业和食品加工业适应气候变化的低碳技术至关重要。碳足迹(CF)分析是评估食品生产和销售对环境影响的重要工具,要求对每种产品从生产到消费的生命周期进行全面评估。这项研究的重点是波兰三家工厂的食糖生产碳足迹,研究技术流程并绘制生产周期的单位流程图。通过分析,开发了一个数据库,用于计算基于产量的 CF。确定的二氧化碳当量为 0.14-0.27 千克二氧化碳当量/千克,平均二氧化碳当量为:工厂 1-0.18 千克二氧化碳当量/千克;工厂 2-0.19 千克二氧化碳当量/千克;工厂 3-0.19 千克二氧化碳当量/千克。持续监测至关重要,可使生产实践适应不断变化的条件,确保快速响应可持续发展的需求。减少糖生产的二氧化碳排放量涉及多项战略,包括采用可持续的种植方法、优化生产流程、使用可再生能源、提高运输效率以及最大限度地减少废物。这些措施共同促进了更环保的制糖生产。通过优先考虑可持续发展和采用创新解决方案,食品行业可以显著减少对环境的影响,应对气候变化和生物多样性丧失的挑战。
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引用次数: 0
In search of poly-3-hydroxybutyrate (PHB): A comprehensive review unveiling applications and progress in fostering a sustainable bio-circular economy 寻找聚 3-羟基丁酸(PHB):全面回顾在促进可持续生物循环经济方面的应用和进展
IF 3.5 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-08-28 DOI: 10.1016/j.fbp.2024.08.011

The escalating demand for economical and durable materials has propelled plastics into an indispensable facet of daily human life, dominating commercial and industrial sectors. The global plastic production of 1.1 billion tons by 2050 exacerbates concerns. The COVID-19 pandemic has further intensified the issue, reaching an alarming 0.3–0.4 billion tons annually. Urgent action is imperative to curtail the drastic environmental impact. Various strategies, particularly microbial involvement in plastic production and degradation, must be implemented to address this. Poly-3-Hydroxybutyrate (PHB) microbial polyesters present a promising alternative to conventional plastics because of their biodegradable nature, thus offering a sustainable solution to plastic pollution. PHBs are employed in divergent industries, including agriculture, medicine, nanotechnology, food, and tissue engineering. This comprehensive review addresses the gap in the literature by encompassing a wide range of topics related to PHBs, their associated enzymes, metabolic pathways, and applications. It also provides an in-depth analysis, highlighting the significance of diverse microbial communities in both the synthesis and degradation of biopolymers. Strategies for augmenting PHA production and leveraging waste products for circular economy initiatives are also discussed, emphasizing the need for innovative solutions to address the global plastic crisis.Top of Form This review highlights two critical strategies for tackling plastic pollution: introducing alternative materials like bioplastics and leveraging biological recycling with microbial assistance. Adopting bio-based circular economy strategies, implementing comprehensive 6 R waste management practices, strengthening plastic pollution regulations, and raising social awareness can significantly improve eco-friendly plastic waste management, diminish pollution, and enhance socio-economic conditions, thus benefiting economies worldwide.

人们对经济耐用材料的需求不断增长,塑料已成为人类日常生活中不可或缺的一部分,在商业和工业领域占据主导地位。到 2050 年,全球塑料产量将达到 11 亿吨,这加剧了人们的担忧。COVID-19 大流行进一步加剧了这一问题,年产量达到惊人的 3-4 亿吨。当务之急是采取紧急行动,减少对环境的严重影响。为此,必须实施各种策略,特别是微生物参与塑料生产和降解的策略。聚 3-羟基丁酸(PHB)微生物聚酯因其可生物降解的特性,成为替代传统塑料的理想选择,从而为塑料污染提供了一种可持续的解决方案。PHB 可用于多种行业,包括农业、医药、纳米技术、食品和组织工程。本综述涵盖了与 PHBs、其相关酶、代谢途径和应用有关的广泛主题,填补了文献空白。它还进行了深入分析,强调了不同微生物群落在生物聚合物合成和降解过程中的重要作用。本综述强调了解决塑料污染问题的两大关键策略:引入生物塑料等替代材料以及利用微生物辅助生物回收。采用以生物为基础的循环经济战略、实施全面的 6 R 废物管理措施、加强塑料污染法规以及提高社会意识,可以显著改善生态友好型塑料废物管理、减少污染并改善社会经济状况,从而惠及全球经济。
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引用次数: 0
Food waste: analysis of the complex and variable composition of a promising feedstock for valorisation 厨余垃圾:分析有望实现资源化的原料的复杂多变成分
IF 3.5 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-08-28 DOI: 10.1016/j.fbp.2024.08.012

There are global concerns about the environmental, social, and economic consequences associated with the generation of food waste. To effectively address this challenge, and particularly to develop food waste valorisation approaches, it is necessary to acquire understanding of the physicochemical and biochemical characteristics of food waste. This study comprises a systematic overview and quantitative assessment of the characteristics of food waste biomass, and this was achieved through a comprehensive literature review. The resulting database encompasses the physicochemical, biochemical, and elemental composition of food waste. The study evaluates food waste variability, analyses correlations between components, and highlights patterns in composition. Overall, food waste is a rather variable material. Typology, collection source and geographical origin of food waste streams are the main contributing factors to variation in physicochemical, biochemical and elemental compositions of food waste, while collection season and storage temperature appear not to be contributing substantially to variation. A clear distinction between plant-based and animal-based food waste biomass can be observed with animal-matter enriched food waste having high contents of protein, lipid and ash, but a low starch content. On the other hand, plant-based food waste can be either high in lignin and low in starch or high in carbohydrates, starch and higher heating value. Fibre content appears an indicative parameter, distinguishing plant from animal enriched food waste, and correlating strongly with lignin-rich, starch-poor plant biomass. The heterogeneity of food waste biomass composition will create challenges in developing and scaling up appropriate food waste management. The current study shows that the analysis of specific food waste parameters, such as fibre content, can be used, to inform the choice of the most appropriate valorisation route.

全球都在关注与食物垃圾产生相关的环境、社会和经济后果。为了有效应对这一挑战,特别是开发厨余垃圾价值化方法,有必要了解厨余垃圾的物理化学和生物化学特征。本研究对厨余生物质的特性进行了系统的概述和定量评估,并通过全面的文献综述实现了这一目标。由此产生的数据库涵盖了厨余垃圾的物理化学、生物化学和元素组成。该研究评估了厨余垃圾的可变性,分析了各成分之间的相关性,并强调了成分的模式。总体而言,厨余垃圾是一种相当多变的材料。泔水流的类型、收集来源和地理来源是导致泔水理化、生化和元素成分变化的主要因素,而收集季节和储存温度似乎对泔水的变化没有实质性影响。植物性厨余生物质和动物性厨余生物质之间有明显区别,富含动物物质的厨余蛋白质、脂类和灰分含量高,但淀粉含量低。另一方面,植物性厨余既可以是木质素含量高、淀粉含量低,也可以是碳水化合物、淀粉含量高、热值高。纤维含量似乎是一个指示性参数,可以区分植物性和动物性厨余垃圾,并与木质素含量高、淀粉含量低的植物生物质密切相关。厨余生物质成分的异质性将为开发和推广适当的厨余管理带来挑战。目前的研究表明,可以利用对特定厨余参数(如纤维含量)的分析,为选择最合适的资源化途径提供信息。
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引用次数: 0
Assessment of a beeswax-packed domestic solar dryer for sustainable bitter gourd drying: An experimental study 评估用于可持续苦瓜干燥的蜂蜡包装家用太阳能干燥器:实验研究
IF 3.5 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-08-24 DOI: 10.1016/j.fbp.2024.08.007

Solar dryers present a clean and affordable solution to food wastage which is one of the biggest concerns of the world. The highest contribution of domestic sector to the global food waste and lack of researches on small-scale solar dryers have created a need to develop sustainable and low cost domestic solar dryers. The present research aims to develop and analyse the output behaviour of a beeswax-packed domestic solar dryer (BDSD) for intermittent bitter gourd drying. The drying kinetics of bitter gourd slices and thermal, economic, environmental and exergetic performances of the BDSD have been evaluated with varying sample masses for passive and active drying conditions. The average final moisture content of the bitter gourd slices for passive and active conditions were found to be 3.26 and 3.93 % (wb), respectively. Midilli-Kucuk model exhibited the strongest fit to the bitter gourd drying behaviour under both the drying conditions. The total moisture evaporation, heat transfer coefficients, thermal efficiency, specific moisture extraction rate, savings for dried bitter gourd slices drying, CO2 mitigation, carbon credit earned and exergy efficiency were found to increase while the drying rate, effective moisture diffusivity, specific energy consumption, costs for bitter gourd slices drying, payback period and energy payback time decreased as the sample mass increased under both drying conditions. The performance of the BDSD was found to be a linear function of sample mass under both drying conditions.

太阳能烘干机为解决全球最令人担忧的粮食浪费问题提供了一种清洁、经济的解决方案。由于家用领域在全球食物浪费中所占比例最高,而且缺乏对小型太阳能干燥机的研究,因此有必要开发可持续的低成本家用太阳能干燥机。本研究旨在开发和分析用于间歇性苦瓜干燥的蜂蜡包装家用太阳能干燥器(BDSD)的输出行为。在被动和主动干燥条件下,对苦瓜片的干燥动力学以及 BDSD 的热、经济、环境和能效性能进行了评估。在被动和主动条件下,苦瓜片的最终平均含水量分别为 3.26% 和 3.93%(湿重)。在两种干燥条件下,Midilli-Kucuk 模型对苦瓜干燥行为的拟合度最高。在两种干燥条件下,总水分蒸发量、传热系数、热效率、特定水分提取率、苦瓜片干燥节省量、二氧化碳减排量、获得的碳信用额和放能效率都有所增加,而干燥速率、有效水分扩散率、特定能耗、苦瓜片干燥成本、投资回收期和能源回收时间则随着样品质量的增加而减少。在两种干燥条件下,BDSD 的性能都是样品质量的线性函数。
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引用次数: 0
Effect of ohmic heating on the extraction of biocompounds from aqueous and ethanolic suspensions of Pavlova gyrans 欧姆加热对从 Pavlova gyrans 的水悬浮液和乙醇悬浮液中提取生物化合物的影响
IF 3.5 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-08-23 DOI: 10.1016/j.fbp.2024.08.008

Electric field technology has been highlighted as a promising strategy in the permeabilization of cell membranes and the corresponding bioactive release. In this work, aqueous and ethanolic suspensions of Pavlova gyrans were subjected to moderate electric fields (MEF) to promote rapid heating due to Ohmic Heating (OH) effect. Two approaches were tested: i) OH assisted extraction in a temperature range between 25 ºC and 85 ºC, and ii) OH pretreatment at 25 ºC and 55 ºC followed by solvent extraction. OH treatment at 55 ºC (performed in less than 10 s) in aqueous suspensions promoted a marked increase in the release of organic matter (2.1-fold), chlorophyll a (41.1-fold) and total carotenoids (56.6-fold) when compared to the freeze-thaw cycles (FTC ≈ 3 hours). OH-pretreated biomass subjected to a two-step passive extraction improved the release of proteins and chlorophylls over incubation (p < 0.05) when compared to untreated biomass. This procedure increased 2.2-, 3.9- and 31.9-fold the content of organic matter, protein and chlorophyll a, respectively, in comparison to the FTC procedure. This work presented the OH effect as a promising strategy for enhanced disruption and release of intracellular microalgae compounds.

电场技术已被视为细胞膜渗透和相应生物活性释放的一种有前途的策略。在这项工作中,将 Pavlova gyrans 的水悬浮液和乙醇悬浮液置于中等电场(MEF)中,以促进欧姆加热(OH)效应引起的快速加热。试验采用了两种方法:i) 在 25 ºC 至 85 ºC 的温度范围内进行 OH 辅助萃取;ii) 在 25 ºC 和 55 ºC 温度范围内进行 OH 预处理,然后进行溶剂萃取。与冻融循环(FTC ≈ 3 小时)相比,在 55 ºC 下对水悬浮液进行 OH 处理(处理时间少于 10 秒)可显著增加有机物(2.1 倍)、叶绿素 a(41.1 倍)和类胡萝卜素总量(56.6 倍)的释放。与未经处理的生物质相比,经过两步被动萃取的 OH 预处理生物质在培养过程中提高了蛋白质和叶绿素的释放量(p < 0.05)。与 FTC 程序相比,该程序使有机物、蛋白质和叶绿素 a 的含量分别增加了 2.2 倍、3.9 倍和 31.9 倍。这项工作表明,OH 效应是增强细胞内微藻化合物的破坏和释放的一种有前途的策略。
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引用次数: 0
Conventionally cooked and UV-A light dehydrated beef jerky: Effects on physicochemical properties 传统烹饪和紫外线 A 光脱水牛肉干:对理化特性的影响
IF 3.5 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-08-22 DOI: 10.1016/j.fbp.2024.08.009

This study compared beef jerky produced by conventional cooking and UV-A light dehydration. The analyses included the isopiestic method, colorimetric analysis, electron microscopy, infrared spectroscopy, and microbial inactivation studies. The primary criterion was a water activity (aw) value of < 0.85 to optimize UV-A dehydration time, taking 4.5 h to achieve this level. UV-A light dehydration resulted in significantly less browning compared to conventional cooking, yielding a duller, less red product (P < 0.05). Electron microscopy showed that UV-A light dehydration maintained a more uniform microstructure, while conventional cooking caused more structural deffects. Microbial inactivation studies demonstrated the antimicrobial effect of UV-A light by reducing accessible water (aw < 0.85). The hygroscopicity of UV-A light dehydrated jerky was similar to conventionally cooked jerky. Overall, UV-A light-dehydrated beef jerky had similar characteristics to conventionally cooked jerky but with notable differences that could appeal to specific consumer preferences.

这项研究比较了传统烹饪法和紫外线 A 光脱水法生产的牛肉干。分析包括等压法、比色分析、电子显微镜、红外光谱和微生物灭活研究。首要标准是水活性(aw)值达到 0.85,以优化紫外线-A 脱水时间,达到这一水平需要 4.5 小时。与传统烹饪相比,紫外线-A 光脱水明显减少了褐变,得到的产品颜色更暗、更不红(P < 0.05)。电子显微镜显示,紫外线-A 光脱水能保持更均匀的微观结构,而传统烹饪会造成更多的结构破坏。微生物灭活研究表明,紫外线-A 光通过减少可获得的水分(aw < 0.85)起到了抗菌作用。紫外线-A 光脱水牛肉干的吸湿性与传统烹饪牛肉干相似。总的来说,紫外线-A 光脱水牛肉干具有与传统熟牛肉干相似的特性,但也有明显的差异,这可能会吸引特定消费者的偏好。
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引用次数: 0
A new enzymatic clarification method for pomegranate juice: Removal of defects and improvement of quality by tannase, lactonase and papain 石榴汁酶解澄清新方法:用单宁酶、乳蛋白酶和木瓜蛋白酶去除缺陷和提高质量
IF 3.5 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-08-21 DOI: 10.1016/j.fbp.2024.08.006

Effects of clarification with “tannase,” “tannase+lactonase,” “papain,” “aspartic protease” and “tannase+lactonase+papain” on defects (turbidity, sediment and color loss) and important constituents (phenolics, anthocyanins, proteins and amino acids) of pomegranate juice (PJ) were investigated. Lactonase [human-derived (H-PON-1 and H-PON-2) and rabbit-derived (R-PON-1, R-PON-2 and R-PON-3)] was used during juice clarification for the first time. Phenolics and amino acids were quantified by HPLC-DAD-MS and HPLC. After clarifications with “tannase (1 g/L)+R-PON-1 (0.33 mg/L)” and “tannase (1 g/L)+R-PON-1 (0.33 mg/L)+papain (10 mg/L)” at 30℃ for 90 min, sediment was completely eliminated during storage at 20℃ for a month. Among these treatments, “tannase+R-PON-1” provided the lowest turbidity formation rate. The most effective parameter to prevent sediment was the ratio of “prolamin to trigalloyl-HHDP-glucose-isomer-2” that should be ≥205.4 (r=–1.000). Moreover, “tannase+R-PON-1” caused 4.3 times lower turbidity than control group after storage. To retain low turbidity (≤10 NTU) during storage, galloyl-glucose-isomer and HHDP-galloyl-hexoside-3 should completely be removed from PJ and, punigluconin and trigalloyl-HHDP-glucose-isomer-2 contents should be ≤0.19 and ≤0.04 mg/L, respectively. Furthermore, “tannase+R-PON-1” provided a significant contribution to anthocyanin copigmentation. The most effective copigments in PJ were HHDP-galloyl-glucuronide, punigluconin, punicalagin-2, punicalagin-3, aspartic acid, glutamic acid and alanine. “Tannase+R-PON-1” is recommended to produce a high-quality PJ.

研究了使用 "单宁酸酶"、"单宁酸酶+乳酸菌素酶"、"木瓜蛋白酶"、"天冬氨酸蛋白酶 "和 "单宁酸酶+乳酸菌素酶+木瓜蛋白酶 "进行澄清对石榴汁(PJ)的缺陷(浑浊度、沉淀物和颜色损失)和重要成分(酚类、花青素、蛋白质和氨基酸)的影响。在果汁澄清过程中首次使用了乳酸菌酶[人源型(H-PON-1 和 H-PON-2)和兔源型(R-PON-1、R-PON-2 和 R-PON-3)]。酚类物质和氨基酸通过 HPLC-DAD-MS 和 HPLC 进行定量。用 "单宁酸酶(1 g/L)+R-PON-1(0.33 mg/L)"和 "单宁酸酶(1 g/L)+R-PON-1(0.33 mg/L)+木瓜蛋白酶(10 mg/L)"在 30℃ 下澄清 90 分钟后,在 20℃ 下贮藏一个月后,沉淀物完全消除。在这些处理中,"单宁酶+R-PON-1 "的浊度形成率最低。防止沉淀物产生的最有效参数是 "丙烯胺与三缩水甘油醚-葡萄糖异构体-2 "的比率,应≥205.4(r=-1.000)。此外,"鞣酸酶+R-PON-1 "贮藏后的浊度比对照组低 4.3 倍。要在贮藏期间保持低浊度(≤10 NTU),应完全去除 PJ 中的五倍子-葡萄糖异构体和 HHDP-五倍子-己糖苷-3,并且普尼格葡糖酸和三倍子-HHDP-葡萄糖异构体-2 的含量应分别≤0.19 和≤0.04 mg/L。此外,"单宁酶+R-PON-1 "对花青素共着色也有重要贡献。PJ 中最有效的花青素共聚物是 HHDP-galloyl-glucuronide、punigluconin、punicalagin-2、punicalagin-3、天冬氨酸、谷氨酸和丙氨酸。建议使用 "单宁酶+R-PON-1 "来生产高质量的 PJ。
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Food and Bioproducts Processing
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