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Robust optimization of regional biomass supply chain system design and operation with data-driven uncertainties 利用数据驱动的不确定性对区域生物质供应链系统的设计和运营进行稳健优化
IF 3.5 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-11-23 DOI: 10.1016/j.fbp.2024.11.021
Xianling Huang , Ling Ji , Yulei Xie , Zhiwei Luo
To address climate change and ensure energy security, biomass energy has become a popular renewable energy alternative to traditional fossil fuels. This study focuses on the strategic planning and tactical management problem of a regional biomass supply chain system with multiple feedstock and final products. A novel SVC-based data-driven robust optimization model is developed to provide the tradeoff solutions under uncertainties. Compared with the traditional robust optimization model, it can better depict the uncertain sets, reduce data redundancy, and provide less conservative strategies for decision makers according to their risk preferences. The proposed model is validated through a case study, Meizhou city in China. The results suggested the optimal investment scale and site for biorefineries and biomass power plants at the strategic planning level, as well as the feedstock supply network, inventory management, and production arrangement at the tactic management level. The optimal robust solutions can be derived by varying the envelope level of the uncertain sets. Moreover, sensitivity analysis is performed to investigate the influences of the variations of key parameters. It is found that the produced bioethanol and electricity lack cost advantages in the current situation, even with a 30 % reduction in main cost parameters. Insufficient biomass yield may require extra capital investment to satisfy bioenergy demand but with a higher supply cost. Thus, a high and long-term subsidy is necessary to facilitate the sustainable development of biomass and the low-carbon transition of the energy sector.
为应对气候变化和确保能源安全,生物质能源已成为替代传统化石燃料的热门可再生能源。本研究重点关注具有多种原料和最终产品的区域生物质供应链系统的战略规划和战术管理问题。研究开发了一种基于 SVC 数据驱动的新型鲁棒优化模型,以提供不确定条件下的权衡解决方案。与传统的稳健优化模型相比,该模型能更好地描述不确定集合,减少数据冗余,并根据决策者的风险偏好为其提供不太保守的策略。通过对中国梅州市的案例研究,对所提出的模型进行了验证。结果从战略规划层面提出了生物精炼厂和生物质发电厂的最佳投资规模和选址,并从战术管理层面提出了原料供应网络、库存管理和生产安排。通过改变不确定集合的包络水平,可以得出最佳稳健解决方案。此外,还进行了敏感性分析,以研究关键参数变化的影响。研究发现,在当前情况下,即使主要成本参数降低 30%,生产的生物乙醇和电力也缺乏成本优势。生物质产量不足可能需要额外的资本投资来满足生物能源需求,但供应成本较高。因此,有必要提供长期的高额补贴,以促进生物质能的可持续发展和能源行业的低碳转型。
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引用次数: 0
Use of liquid nitrogen as pistachio peeling agent: quality parameters of kernel and skins (testa) 使用液氮作为开心果去皮剂:果仁和果皮(种皮)的质量参数
IF 3.5 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-11-22 DOI: 10.1016/j.fbp.2024.11.023
Fabiola Pesce, Lucia Parafati, Biagio Fallico, Rosa Palmeri
Nuts peeling involves the use of high volumes of hot water (90–95°C), which results in a quality loss of the final product with a significant environmental impact. The present study exploits the use of cold nitrogen gases in order to perform an alternative technique of Liquid Nitrogen Peeling (LNP). Peeled pistachio (kernel) and skin (testa) obtained from LNP were compared with those obtained using the Traditional Blanching Peeling (TBP) method. Commercial pistachios present a peroxide value equal to 4.59±0.40 meq O2/kg of oil. After peeling process LNP sample showed a significantly (p < 0.05) lower peroxide value (PV), equal to 5.27±0.38 meq O2/kg of oil, in comparison to that subjected to TBP that registered the value of 10.47±1.07 meq O2/kg of oil. Moisture content is strictly connected to the stability of the final product. In our LNP samples, pistachio kernels recorded a moisture value significantly lower (p < 0.05) compared to TBP sample, and evidenced superior colorimetric characteristics. Moreover, the resulting pistachio skin from LNP had higher values of bioactive compounds and antioxidant activity. Total anthocyanins content was almost three times higher (26.03±1.61 mg Cyanidin-3-O-glucoside eq (Cya-GE) /g sample dry weight) than that recovered from skin obtained after TBP (8.33±0.70 mg Cya-GE/g sample dry weight). Results suggest this by-product could be a resource with healthy properties that can be used in food formulations.
坚果去皮需要使用大量热水(90-95°C),这会导致最终产品的质量下降,并对环境造成严重影响。本研究利用低温氮气来替代液氮去皮(LNP)技术。将通过液氮去皮法获得的开心果果核和果皮与通过传统焯水去皮法(TBP)获得的果核和果皮进行了比较。商用开心果的过氧化值为 4.59±0.40 meq O2/kg 油。经过去皮处理后,LNP 样品的过氧化值 (PV) 明显降低(p < 0.05),为 5.27±0.38 meq O2/kg,而 TBP 样品的过氧化值为 10.47±1.07 meq O2/kg。水分含量与最终产品的稳定性密切相关。在我们的 LNP 样品中,与 TBP 样品相比,开心果核仁的水分值明显较低(p < 0.05),而且色度特性也更好。此外,从 LNP 中提取的开心果皮具有更高的生物活性化合物值和抗氧化活性。花青素总含量(26.03±1.61 毫克花青素-3-O-葡萄糖苷当量(Cya-GE)/克样品干重)比从 TBP 后的皮中回收的含量(8.33±0.70 毫克花青素-3-O-葡萄糖苷当量/克样品干重)高出近三倍。结果表明,这种副产品可能是一种具有健康特性的资源,可用于食品配方中。
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引用次数: 0
Process synthesis, design and techno-economic assessment of bio-succinic acid production 生物琥珀酸生产工艺合成、设计及技术经济评价
IF 3.5 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-11-21 DOI: 10.1016/j.fbp.2024.11.020
George Melitos , Nikiforos Misailidis , Demetri Petrides , Michael C. Georgiadis
This work investigates the potential of different fermentation feedstocks towards succinic acid production. Three alternative flowsheets were constructed and modelled in SuperPro Designer™, utilizing different fermentation substrates: crude glycerol (a by-product of the biodiesel industry), a commercial 95 % glucose syrup and pure glycerol. Techno-economic analysis of the modelled plants showed that all three potential investments can be economically profitable, with the crude glycerol case as the most promising one. The succinic acid production cost for the crude glycerol scenario is estimated at 3.57 $/kg, while a thorough study of the capacity effect to the economics showed that it could drop as low as $2.6 $/kg. Parametric analysis and optimization performed on a flowsheet level, indicated opportunities for lowering the production cost down to 3 $/kg, a value that could pave the framework for a further potential decrease in the selling price of the product.
本研究探讨了不同发酵原料对琥珀酸生产的潜力。利用不同的发酵底物:粗甘油(生物柴油工业的副产品),商用95% %的葡萄糖浆和纯甘油,在SuperPro Designer™中构建和建模了三个可选的流程。模拟装置的技术经济分析表明,三种潜在的投资都具有经济效益,其中粗甘油是最有希望的投资。在粗甘油的情况下,琥珀酸的生产成本估计为3.57美元/公斤,而对产能效应的深入研究表明,成本可以降至2.6美元/公斤。在流程层面上进行的参数分析和优化表明,有机会将生产成本降至3美元/公斤,这一价值可以为进一步降低产品销售价格奠定基础。
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引用次数: 0
Development and optimization of a two-step co-extraction process for the recovery of pumpkin seed oil and in-situ enrichment with β-carotene compounds from pumpkin peel 南瓜皮中β-胡萝卜素化合物的原位富集和两步萃取工艺的开发与优化
IF 3.5 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-11-21 DOI: 10.1016/j.fbp.2024.11.015
Lindah Phambala Chifomboti , Annie F.A. Chimphango
Vegetable oil industries are interested in producing healthy, high-quality, and cost-effective edible oils. This necessitates greener sources and effective oil enrichment strategies involving non-toxic and natural antioxidants. Thus, this study developed a novel one-pot vegetable oil natural pigmentation strategy to enable the in-situ cold press extraction and pigmentation of the vegetable oils while enhancing yields. The study co-pressed microwave pretreated pumpkin seeds and peels (PSP),3 producing pumpkin seed oil (PSO)4 which facilitated the co-extraction of lipophilic β-carotene compounds from the peels in a structured bed. The processing conditions were optimised for yields through Box-Behnken Design experiments, which varied seed-to-peel ratio (50 – 90 % w/w), microwave power (200 – 600 Watts), irradiation time (180 – 240 sec), and pressure (10 – 20 MPa). Optimal conditions (80 % w/w seeds,5 600 Watts, 240 sec, 20 MPa) recovered 73.58 % oil and 5.48 ± 0.33 mg β-carotene /100 g biomass. PSP oils with natural β-carotene (0.75±0.02 mg β-carotene/100 g oil) pigmentation was more oxidative stable (based on unsaturated fatty acids content) at elevated temperatures (180 °C, 6 h), outperforming unpigmented seed oils (0.47±0.01 mg/100 g oil) despite the reduced yield. Therefore, microwave pretreatment and one-pot co-extraction have the potential to produce high-quality edible oil from vegetable residues with reduced processing steps. Thus, promoting material circularity in food processing.

Industrial relevance

The utilization of a microwave-assisted mechanical processing scheme comprising a mixture of pumpkin seeds and peel provides a potential multi-product pathway for obtaining maximum value from pumpkin residues. This approach offers notable industrial benefits and applications:
  • I.
    Microwave heating shows promising potential in enhancing the extractability of pumpkin seed oil and β-carotene compounds from the peel. Thus, rendering it a viable pre-treatment method for vegetable oil production and in-situ recovery of natural antioxidants.
  • II.
    The layered structured-bed presents a co-extraction technique (for pumpkin seed oil and β-carotene) with fewer extraction steps compared to the multi-step, energy-intensive conventional oil enrichment processes.
植物油行业对生产健康、高质量、高性价比的食用油很感兴趣。这就需要更环保的资源和有效的石油富集策略,包括无毒和天然抗氧化剂。因此,本研究开发了一种新的一锅植物油天然色素沉着策略,可以在提高产量的同时实现植物油的原位冷压提取和色素沉着。本研究采用微波预处理南瓜籽和南瓜皮(PSP)共压,得到南瓜籽油(PSO)4,使其在结构床上从南瓜籽皮中共提取亲脂性β-胡萝卜素化合物。通过Box-Behnken设计实验,优化了种子与果皮比(50 - 90 % w/w)、微波功率(200 - 600瓦)、辐照时间(180 - 240 秒)和压力(10 - 20 MPa)的加工条件。最佳条件(80 % w/w种子,5 600瓦,240 秒,20 MPa)可回收73.58 %油和5.48±0.33 mg β-胡萝卜素/100 g生物量。含有天然β-胡萝卜素(0.75±0.02 mg β-胡萝卜素/100 g油)色素沉着的PSP油在高温(180°C, 6 h)下氧化稳定性更好(基于不饱和脂肪酸含量),尽管产量降低,但优于未着色的种子油(0.47±0.01 mg/100 g油)。因此,微波预处理和一锅共浸有可能减少加工步骤,从蔬菜残渣中生产出高质量的食用油。从而促进食品加工中的材料循环。利用微波辅助的机械加工方案,包括南瓜籽和瓜皮的混合物,为从南瓜残留物中获得最大价值提供了一个潜在的多产品途径。这种方法提供了显著的工业效益和应用:微波加热在提高南瓜籽油和β-胡萝卜素化合物的提取率方面显示出良好的潜力。因此,使其成为一种可行的植物油生产和天然抗氧化剂原位回收的预处理方法。ii .层状结构床提供了一种共提取技术(用于南瓜籽油和β-胡萝卜素),与多步骤、能源密集型的常规油富集工艺相比,提取步骤更少。
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引用次数: 0
Isolation, identification, and characterization of Bacillus subtilis SMP-2 from panitenga and exploring its potential for biosurfactant production 从 Panitenga 分离、鉴定和表征枯草芽孢杆菌 SMP-2 并探索其生产生物表面活性剂的潜力
IF 3.5 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-11-19 DOI: 10.1016/j.fbp.2024.11.018
Smrity Sonbhadra, Lalit M. Pandey
A traditional Assamese fermented food, Panitenga, was explored for potential probiotic isolation. A potent bacterial strain exhibiting biosurfactant production ability was isolated, characterized, and identified as Bacillus subtilis SMP-2. Environmental parameters were optimized for maximum cell growth and biosurfactant production that was found to be pH 6, 30°C, 130 rpm, with glycerol as the carbon (C) source, yeast extract as the nitrogen (N) source, and a C/N ratio of 3:1. At these optimized conditions, the strain yielded 8.13 ± 0.9 g/L of lipopeptide biosurfactant. This biosurfactant remarkably reduced the surface tension of water from 72 mN/m to 37 ± 0.36 mN/m, possessed a low critical micelle concentration (CMC) of 125 mg/L, and exhibited antimicrobial properties. It persisted to be stable across a wide range of physical conditions, including variations in pH, temperature, and salinity. The significantly high production yield and noteworthy properties of the biosurfactant from Bacillus subtilis SMP-2 make it a promising candidate for applications in microbial-enhanced oil recovery (MEOR), bioremediation of oil spills, production of cosmetics and pharmaceuticals, contributing to economic growth and sustainable development.
研究人员对一种传统的阿萨姆发酵食品 Panitenga 进行了潜在益生菌分离研究。分离、鉴定并确定了一种具有生产生物表面活性剂能力的强效细菌菌株,即枯草芽孢杆菌 SMP-2。为实现最大的细胞生长和生物表面活性剂产量,对环境参数进行了优化,发现 pH 值为 6,温度为 30°C,转速为 130 rpm,碳(C)源为甘油,氮(N)源为酵母提取物,C/N 比为 3:1。在这些优化条件下,该菌株产生了 8.13 ± 0.9 克/升的脂肽生物表面活性剂。这种生物表面活性剂显著降低了水的表面张力,从 72 mN/m 降至 37 ± 0.36 mN/m,临界胶束浓度(CMC)低至 125 mg/L,并具有抗菌特性。它在各种物理条件下(包括 pH 值、温度和盐度变化)都能保持稳定。从枯草芽孢杆菌 SMP-2 中提取的生物表面活性剂产量很高,而且具有显著的特性,因此有望应用于微生物强化采油(MEOR)、溢油的生物修复、化妆品和药品的生产,从而促进经济增长和可持续发展。
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引用次数: 0
Predictive Modeling of Emulsion Stability and Drop Characteristics Using Machine Learning: A Study on Surfactant Influence and Time Dynamics 利用机器学习对乳液稳定性和液滴特性进行预测建模:表面活性剂影响和时间动态研究
IF 3.5 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-11-19 DOI: 10.1016/j.fbp.2024.11.019
Hasnain Ahmad Saddiqi , Asmat Ullah , Zainab Javed , Qazi Muhammad Ali , Muhammad Bilal Jan , Iftikhar Ahmad , Farooq Ahmad
This study explores the application of empirical and machine learning techniques to assess the impact of surfactants and time on the stability of oil-water emulsions and the characteristics of droplets. It utilizes a novel machine learning approach to forecast cumulative mass percentages by considering parameters such as drop size and time. The actual data was at 1st, 30, and 60 minutes after emulsion preparation and were forecasted up to 180 minutes with a Long-Short Term Memory (LSTM) machine learning model. The model demonstrates promising results in capturing the intricate relationships characterized by achieving an R-Squared (R2) score of 0.898 and Mean Squared Error (MSE) 0.00466. Under similar conditions and analysis, the results predicted for all three surfactants Gum Arabic (GA), Tween-20 (T20), and Poly Vinyl Alcohol (PVA) demonstrated similar behavior. Overall change in cumulative mass is lower confirming emulsion stability; however, at time stamps coalescence occurs, that can be neglected due to little impact. The results also show that interfacial tension is directly related to emulsion stability. Gum Arabic having highest interfacial tension (16mN/m) resulted in the most stable emulsion as compared to lowest interfacial tension surfactant Tween-20 (4mN/m). It is important to acknowledge certain limitations such as variations in surfactant concentration, temperature fluctuations, and shear forces, which may impact the experimental results and model performance. In conclusion, the current finding indicates that predictive modeling with LSTM in understanding emulsion dynamics is providing a foundation for future developments aimed at improving product performance and stability in a variety of industrial sectors like oil/gas, food and pharmaceutical.
本研究探索了经验和机器学习技术的应用,以评估表面活性剂和时间对油水乳剂稳定性和液滴特性的影响。它采用了一种新颖的机器学习方法,通过考虑液滴大小和时间等参数来预测累积质量百分比。实际数据是乳液制备后 1 分钟、30 分钟和 60 分钟的数据,并通过长短期记忆(LSTM)机器学习模型预测到 180 分钟。该模型在捕捉错综复杂的关系方面取得了可喜的成果,R-平方 (R2) 为 0.898,平均平方误差 (MSE) 为 0.00466。在相似的条件和分析下,阿拉伯树胶 (GA)、吐温-20 (T20) 和聚乙烯醇 (PVA) 这三种表面活性剂的预测结果表现出相似的行为。累积质量的总体变化较低,这证实了乳液的稳定性;不过,在发生凝聚的时间戳上,由于影响很小,可以忽略不计。结果还表明,界面张力与乳液稳定性直接相关。与界面张力最低的表面活性剂吐温-20(4mN/m)相比,界面张力最高(16mN/m)的阿拉伯树胶产生的乳液最稳定。必须承认某些局限性,如表面活性剂浓度、温度波动和剪切力的变化可能会影响实验结果和模型性能。总之,目前的研究结果表明,利用 LSTM 建立预测模型来了解乳液动力学,为今后的开发奠定了基础,旨在提高石油/天然气、食品和制药等多个工业领域的产品性能和稳定性。
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引用次数: 0
All-wood-based hybrid membrane derived from waste sawdust for efficient emulsion separation 利用废锯末制成的全木质混合膜实现高效乳液分离
IF 3.5 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-11-19 DOI: 10.1016/j.fbp.2024.11.017
Haonan Wu , Hao Chen , Xingqi Shao , Xejie Yue , Jie Sun , Tao Zhang , Fengxian Qiu
Oriented toward the demand for safe and sustainable oily wastewater separation, biomass-based composite membranes have received widespread attention due to the advantaged properties of green biodegradability, multifunctionality and easy modification, offering great application potentials in wastewater treatment. However, present studies still need to be done to enhance separation efficiency and to address the potential environmental risks from synthetic nanomaterials in biomass-based composite membranes. Herein, this work presented an “split and reorganization” strategy to prepare an all-biomass-based hybrid membrane for efficient emulsion separation using waste sawdust as raw materials, inspired by whole wheat bread. In this strategy, wood cellulose was extracted from waste sawdust via alkali elutriation, and lignin microparticles were prepared by hydrothermal process using black liquor formed from the extracting process of wood cellulose. Then, the all-wood-based hybrid membrane with super-wettability was fabricated for emulsion separation via vacuum-filtration of wood cellulose and lignin microparticles suspension. The lignin microparticles were uniformly distributed inside the all-wood-based hybrid membrane, which enhanced the surface roughness and endowed exceptional superhydrophilic/underwater super-oleophobic properties of the membrane. The obtained hybrid membrane exhibited superhydrophilicity with a water contact angle of 0° and underwater superoleophobicity with an oil contact angle of 140°. It can effectively separate oil-in-water emulsions with permeances up to 6673 L·m−2·h−1 and high separation efficiency of greater than 98.8 %. More importantly, all-wood-based hybrid membrane demonstrated excellent demulsification and cycle ability after 10 cycles, which match well with the requirements for industrial oily wastewater. This study shows that the developed all-wood-based hybrid membrane and corresponding design strategy can be extended for preparing other biomass-based materials for applications in research and industrial fields.
为满足安全、可持续的含油废水分离需求,生物质基复合膜以其绿色生物降解性、多功能性和易改性等优点受到广泛关注,在废水处理领域具有巨大的应用潜力。然而,如何提高分离效率并解决生物质复合膜中合成纳米材料可能带来的环境风险,仍有待进一步研究。在此,本研究受全麦面包的启发,提出了一种 "拆分重组 "策略,利用废锯末作为原料,制备一种用于高效乳液分离的全生物质基复合膜。在这一策略中,通过碱洗提法从废锯末中提取木纤维素,并利用提取木纤维素过程中形成的黑液通过水热法制备木质素微粒。然后,通过真空过滤木纤维素和木质素微颗粒悬浮液,制造出具有超润湿性的全木基混合膜,用于乳液分离。木质素微粒均匀地分布在全木基混合膜内,增强了膜的表面粗糙度,赋予了膜优异的超亲水/水下超疏油性。所获得的混合膜具有超亲水性(水接触角为 0°)和水下超疏油性(油接触角为 140°)。它能有效分离水包油型乳液,渗透率高达 6673 L-m-2-h-1,分离效率高达 98.8%以上。更重要的是,全木质混合膜在 10 次循环后表现出优异的破乳化和循环能力,完全符合工业含油废水的要求。这项研究表明,所开发的全木基混合膜及相应的设计策略可扩展用于制备其他生物质基材料,并应用于科研和工业领域。
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引用次数: 0
Effect of Dendrobium nobile Lindl. on volatile flavor substances and nonvolatile metabolites of rice wine fermented by Saccharomyces cerevisiae and Wickerhamomyces anomalus monocultures 金钗石斛对酿酒酵母和酿酒酵母单培养基发酵米酒中挥发性风味物质和非挥发性代谢物的影响
IF 3.5 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-11-19 DOI: 10.1016/j.fbp.2024.11.016
Zhongxia Guo , Lin Zhang , Lanyan Cen, Yifeng Dai, Shuyi Qiu, Xiangyong Zeng, Xiaodan Wang, Chaoyang Wei
In order to investigate the effect of Dendrobium nobile Lindl. (DNL) on the quality of rice wine fermented by Saccharomyces cerevisiae (DnSC) and Wickerhamomyces anomalus (DnWA), respectively, the physicochemical indexes, active ingredients, free amino acids, volatile flavor substances and nonvolatile metabolites of rice wine based on chemical methods, GC-MS and nontargeted metabolomics were studied. Results showed that the addition of DNL had a significant effect on the contents of physicochemical indicators. Compared with the fermented rice wine without the addition of DNL, the contents of active ingredients, free amino acids and volatile flavor components in DnSC and DnWA were all significantly increased. Among them, compared with SC, the contents of polysaccharides, flavonoids, total phenols and free amino acids in DnSC rose by 46.67 %, 27.07 %, 20.45 % and 50.14 % respectively. And compared with WA, these active ingredients in DnWA rose by 25.45 %, 20.13 %, 10.77 % and 46.01 % respectively. Notably, after adding DNL, the content of dendrobine in rice wine increased from 0 to 14.53 PPM (DnSC) and 11.41 PPM (DnWA) respectively. The volatile flavor compounds in DnWA rice wine were the most abundant (45 kinds), among which 14 kinds of alcohols were detected. The content of volatile flavor compounds in DnSC rice wine was the highest (45.45 mg/mL), among which 18 kinds of esters were detected. There were significant differences in flavor compounds between different rice wines, which give different aromatic styles and characteristics. Metabolomics analysis showed that the overall expression of metabolic pathways in rice wine was almost up-regulated by the addition of DNL, which resulted more metabolites in rice wine. The metabolic pathways that caused significant differences in Sc-fermented rice wine were the metabolism of nicotinate and nicotinamide and the metabolism of glyoxylate and dicarboxylate, while in Wa-fermented rice wine, they were the metabolism of phenylalanine and the metabolism of galactose. These results provide a theoretical basis for the development of DNL and its application in fermented rice wine.
为探讨金钗石斛(DNL)对酿酒酵母(DnSC)和酿酒酵母(DnWA)发酵米酒品质的影响,采用化学法、气相色谱-质谱(GC-MS)和非靶向代谢组学方法,分别对米酒的理化指标、有效成分、游离氨基酸、挥发性风味物质和非挥发性代谢物进行了研究。结果表明,添加 DNL 对理化指标的含量有显著影响。与未添加 DNL 的发酵米酒相比,DnSC 和 DnWA 中有效成分、游离氨基酸和挥发性风味成分的含量均显著增加。其中,与 SC 相比,DnSC 中多糖、类黄酮、总酚和游离氨基酸的含量分别增加了 46.67%、27.07%、20.45% 和 50.14%。与 WA 相比,DnWA 中的这些有效成分分别增加了 25.45 %、20.13 %、10.77 % 和 46.01 %。值得注意的是,添加 DNL 后,米酒中的石斛碱含量分别从 0 增加到 14.53 PPM(DnSC)和 11.41 PPM(DnWA)。DnWA 米酒中的挥发性风味化合物含量最高(45 种),其中检出 14 种醇。DnSC 米酒中的挥发性风味化合物含量最高(45.45 毫克/毫升),其中检测到 18 种酯。不同米酒的风味化合物存在明显差异,呈现出不同的香气风格和特征。代谢组学分析表明,DNL 的添加几乎上调了米酒中代谢途径的整体表达,从而增加了米酒中的代谢物。在 Sc 发酵的米酒中,造成显著差异的代谢途径是烟酸和烟酰胺的代谢、乙醛酸和二羧酸的代谢,而在 Wa 发酵的米酒中,则是苯丙氨酸的代谢和半乳糖的代谢。这些结果为 DNL 的开发及其在发酵米酒中的应用提供了理论依据。
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引用次数: 0
Effect of air velocity on airborne ultrasound application in pork liver drying 风速对机载超声在猪肝干燥中的应用的影响
IF 3.5 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-11-17 DOI: 10.1016/j.fbp.2024.11.014
Eduardo A. Sánchez-Torres , Esperanza Dalmau , Anabella S. Giacomozzi , Jose Benedito , José Bon , José V. García-Pérez
Airborne ultrasound is considered a feasible emerging technology for the intensification of the drying of biological materials due to its mild thermal effect avoiding the degradation of heat sensitive compounds. The effects brought about by airborne ultrasound on the drying are largely dependent on both the product structure itself and the process variables used. Thus, the present study examines the effect of the air velocity and the application of airborne ultrasound during the low-temperature convective drying of pork liver. For this purpose, drying experiments were carried out at 50 °C on pork liver cylinders using different air velocities, ranging from 1 to 6 m·s−1, without (AIR) and with ultrasound application (US). The modelling of the drying kinetics was approached using the diffusion theory, evaluating both the contribution of external convection and shrinkage. The experimental results demonstrated that the application of airborne ultrasound only increased the drying rate at air velocities below 3 m·s−1, shortening the drying time by up to 30 %.
航空超声由于其热效应温和,避免了热敏性化合物的降解,被认为是一种可行的加强生物材料干燥的新兴技术。空气超声对干燥的影响在很大程度上取决于产品本身的结构和所使用的工艺变量。因此,本研究考察了在猪肝低温对流干燥过程中风速的影响以及机载超声的应用。为此,在50°C的条件下,在猪肝气瓶上进行了不同风速(1至6 m·s−1)的干燥实验,不使用(air)和使用超声波(US)。利用扩散理论建立了干燥动力学模型,评估了外部对流和收缩对干燥动力学的贡献。实验结果表明,机载超声仅在3 m·s−1以下的风速下提高了干燥速率,使干燥时间缩短了30% %。
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引用次数: 0
Effect of solid-state fermentation on mineral binding efficiency of chickpea protein: Characterization and in-vitro mineral uptake 固态发酵对鹰嘴豆蛋白矿物质结合效率的影响:表征和体外矿物质吸收
IF 3.5 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-11-15 DOI: 10.1016/j.fbp.2024.11.013
Nikhil Dnyaneshwar Patil , Ankur Kumar , Minaxi Sharma , Aarti Bains , Kandi Sridhar
Iron deficiency is a prevalent global health concern, especially in populations lacking diverse nutrient sources. Chickpeas, rich in both protein and iron, face limitations in iron bioavailability due to anti-nutritional factors and low iron complex solubility. In this regard, solid-state fermentation offers promise in enhancing plant-based food nutrition. Therefore, this study examined the impact of fermentation on chickpea protein's mineral binding, focusing on iron complexation. Comparing native chickpea protein-iron complex (NCP-Fe) and fermented chickpea protein-iron complex using Aspergillus awamori (FCP90-Fe), significant improvements were noted after 90 h of fermentation. Protein content and solubility in FCP90-Fe increased by 14.77 % and 22.70 %, respectively. Structural alterations induced by A. awamorai were evident through Fourier transform infrared spectroscopy and thermogravimetric analysis. Functional attributes such as protein solubility (18.91 %), oil (23.60 %), and water holding capacity (19.17 %) also improved in FCP90-Fe, indicating enhanced food application potential. Additionally, FCP90-Fe exhibited a 31.74 % increase in iron content and significantly higher mineral bioavailability, with enhancements of 21.99 % and 59.90 % compared to NCP-Fe. In vitro studies demonstrated increased iron transportation, retention, and uptake by 11.07 %, 10.42 %, and 7.09 %, respectively, underscoring improved iron bioavailability from fermented chickpea protein. Moreover, FCP90-Fe notably elevated ferritin synthesis levels, suggesting enhanced iron storage capacity within cells, with a 62.66 % increase in ferritin content per mg cell protein and a 39.59 % increase per gram sample compared to NCP-Fe. This study emphasizes the considerable impact of fermentation on the chickpea protein iron complex. It increases its mineral bioavailability, iron uptake, digestibility, and mineral bioavailability.
缺铁是全球普遍关注的健康问题,尤其是在缺乏多种营养来源的人群中。鹰嘴豆富含蛋白质和铁,但由于抗营养因素和铁复合物溶解度低,其铁的生物利用率受到限制。因此,固态发酵有望提高植物性食品的营养价值。因此,本研究考察了发酵对鹰嘴豆蛋白矿物质结合力的影响,重点关注铁的复合物。将原生鹰嘴豆蛋白-铁复合物(NCP-Fe)与利用 awamori 黑曲霉发酵的鹰嘴豆蛋白-铁复合物(FCP90-Fe)进行比较,发现发酵 90 小时后,两者的结合力显著提高。FCP90-Fe 中的蛋白质含量和可溶性分别增加了 14.77 % 和 22.70 %。通过傅里叶变换红外光谱和热重分析,A. awamorai 引起的结构变化非常明显。FCP90-Fe 的蛋白质溶解度(18.91%)、油脂(23.60%)和持水率(19.17%)等功能特性也得到了改善,这表明其食品应用潜力得到了提高。此外,与 NCP-Fe 相比,FCP90-Fe 的铁含量增加了 31.74%,矿物质生物利用率显著提高,分别提高了 21.99% 和 59.90%。体外研究表明,铁的运输、保留和吸收分别增加了 11.07 %、10.42 % 和 7.09 %,这表明发酵鹰嘴豆蛋白提高了铁的生物利用率。此外,与 NCP-Fe 相比,FCP90-Fe 显著提高了铁蛋白合成水平,表明细胞内的铁储存能力增强,每毫克细胞蛋白中的铁蛋白含量增加了 62.66%,每克样品中的铁蛋白含量增加了 39.59%。这项研究强调了发酵对鹰嘴豆蛋白铁复合物的巨大影响。它提高了鹰嘴豆的矿物质生物利用率、铁吸收率、消化率和矿物质生物利用率。
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引用次数: 0
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Food and Bioproducts Processing
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