Ultrasensitive detection of interleukin-6 enabled by three-dimensional spatial amplification

IF 6.5 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Sensors and Actuators Reports Pub Date : 2024-06-10 DOI:10.1016/j.snr.2024.100213

Masaki Yamaguchi , Vivek Shetty

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Abstract

Accurately measuring interleukin-6 (IL-6) levels is crucial in both clinical medicine and research due to its role in various physiological and pathological processes. We present proof-of-concept of a novel three-dimensional (3D) spatial amplification method for developing highly sensitive and miniaturized IL-6 biosensors. We designed an IL-6 immunosensor platform utilizing a unique 3D microfluidic structure fabricated with femtosecond-pulsed laser processing. This design features a porous transducer element with over 4500 microcavities, thereby increasing the reaction area by 13-fold compared to traditional 2D designs while maintaining transparency. By combining this 3D structure with a competitive antigen-antibody reaction, the sensor achieved an exceptional limit of detection of 0.27 pg/mL for IL-6 in human blood samples. Additionally, we demonstrated effective control of liquid flow within the porous element using a reduction of pressure and centrifugation speeds. This 3D spatial amplification method offers a promising approach for developing highly sensitive and compact IL-6 biosensors. Such miniaturized sensors hold potential for point-of-care testing devices, enabling convenient and timely IL-6 analysis.

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利用三维空间放大技术实现白细胞介素-6 的超灵敏检测

由于白细胞介素-6（IL-6）在各种生理和病理过程中的作用，准确测量其水平在临床医学和研究中都至关重要。我们介绍了一种新型三维（3D）空间放大方法的概念验证，该方法可用于开发高灵敏度和微型化的 IL-6 生物传感器。我们设计了一种 IL-6 免疫传感器平台，利用飞秒脉冲激光加工制造的独特三维微流体结构。这种设计的特点是多孔换能器元件具有 4500 多个微腔，因此与传统的二维设计相比，反应面积增加了 13 倍，同时保持了透明度。通过将这种三维结构与竞争性抗原-抗体反应相结合，该传感器对人体血液样本中 IL-6 的检测限达到了 0.27 pg/mL。此外，我们还通过降低压力和离心速度，证明了多孔元件内液体流动的有效控制。这种三维空间放大方法为开发高灵敏度的小型 IL-6 生物传感器提供了一种可行的方法。这种微型传感器有望用于护理点检测设备，实现方便及时的 IL-6 分析。

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来源期刊

Sensors and Actuators Reports Multiple-

CiteScore

9.60

自引率

0.00%

发文量

审稿时长

49 days

期刊介绍： Sensors and Actuators Reports is a peer-reviewed open access journal launched out from the Sensors and Actuators journal family. Sensors and Actuators Reports is dedicated to publishing new and original works in the field of all type of sensors and actuators, including bio-, chemical-, physical-, and nano- sensors and actuators, which demonstrates significant progress beyond the current state of the art. The journal regularly publishes original research papers, reviews, and short communications. For research papers and short communications, the journal aims to publish the new and original work supported by experimental results and as such purely theoretical works are not accepted.