Sensors and Actuators Reports最新文献

英文中文

Dual-mode ratiometric electrochemical and turn-on fluorescent probe for reliably detecting H2O2 in Parkinson's disease serum

IF 6.5 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports

Pub Date : 2025-02-14 DOI: 10.1016/j.snr.2025.100305

Hui Dong , Linlin Zheng , Zuying Wang , Ke Xu , Wentian Chen , Zi Liu , Zhu Chang , Yanli Zhou , Xu Zhu , Yintang Zhang , Maotian Xu

Accurate diagnosis and effective treatment of Parkinson's disease (PD) rely on the accurate detection of the corresponding biomarkers. The build-up of reactive oxygen species (ROS), for instance, hydrogen peroxide (H₂O₂), resulting in oxidative stress inside cells is closely related to the pathogenesis of PD. In this study, two compounds, Re-B and Re-OB, were synthesized through a simple one-step reaction. Upon reacting with H₂O₂, the borate ester group in Re-B is selectively cleaved, restoring the phenol group. This leads to both a dual-signal ratiometric electrochemical response and a ``turn-on'' fluorescence effect. The Re-B probe demonstrates a clear ``turn-on'' electrochemical current response at −311.1 mV, followed by a ``turn-off'' response at −257.6 mV when interacting with H₂O₂ on a single-walled carbon nanotube-modified screen-printed carbon electrode (SPCE/SWCNTs). Concurrently, the fluorescence spectrum shows a ``turn-on'' response with an emission peak at 586 nm. The fluorescence and ratiometric electrochemical methods developed in this study show exceptionally high sensitivity and selectivity for H₂O₂, with detection limits as low as 0.5 μM for the electrochemical method and 0.1 μM for fluorescence. The consistency between the results from both electrochemical and fluorescence quantification of H₂O₂ levels in serum underscores its reliability. This approach has significant potential as a point-of-care diagnostic device for PD early detection, providing valuable information for timely diagnosis and treatment.

{"title":"Dual-mode ratiometric electrochemical and turn-on fluorescent probe for reliably detecting H2O2 in Parkinson's disease serum","authors":"Hui Dong , Linlin Zheng , Zuying Wang , Ke Xu , Wentian Chen , Zi Liu , Zhu Chang , Yanli Zhou , Xu Zhu , Yintang Zhang , Maotian Xu","doi":"10.1016/j.snr.2025.100305","DOIUrl":"10.1016/j.snr.2025.100305","url":null,"abstract":"<div><div>Accurate diagnosis and effective treatment of Parkinson's disease (PD) rely on the accurate detection of the corresponding biomarkers. The build-up of reactive oxygen species (ROS), for instance, hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), resulting in oxidative stress inside cells is closely related to the pathogenesis of PD. In this study, two compounds, Re-B and Re-OB, were synthesized through a simple one-step reaction. Upon reacting with H<sub>2</sub>O<sub>2</sub>, the borate ester group in Re-B is selectively cleaved, restoring the phenol group. This leads to both a dual-signal ratiometric electrochemical response and a ``turn-on'' fluorescence effect. The Re-B probe demonstrates a clear ``turn-on'' electrochemical current response at −311.1 mV, followed by a ``turn-off'' response at −257.6 mV when interacting with H<sub>2</sub>O<sub>2</sub> on a single-walled carbon nanotube-modified screen-printed carbon electrode (SPCE/SWCNTs). Concurrently, the fluorescence spectrum shows a ``turn-on'' response with an emission peak at 586 nm. The fluorescence and ratiometric electrochemical methods developed in this study show exceptionally high sensitivity and selectivity for H<sub>2</sub>O<sub>2</sub>, with detection limits as low as 0.5 μM for the electrochemical method and 0.1 μM for fluorescence. The consistency between the results from both electrochemical and fluorescence quantification of H<sub>2</sub>O<sub>2</sub> levels in serum underscores its reliability. This approach has significant potential as a point-of-care diagnostic device for PD early detection, providing valuable information for timely diagnosis and treatment.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100305"},"PeriodicalIF":6.5,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143429233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ratiometric fluorescent biosensors for quantitative lactic acid detection using CdTe@CdS quantum dots and lactate oxidase

IF 6.5 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports

Pub Date : 2025-02-11 DOI: 10.1016/j.snr.2025.100303

Giuliana Grasso , Riccardo Scarfiello , Francesco Colella , Valentina Onesto , Stefania Forciniti , Giuseppe Gigli , Luigi Carbone , Loretta L. del Mercato

Lactic acid plays a crucial role in various physiological processes, particularly in cellular metabolism and muscle activity, as it is a key marker of anaerobic respiration and metabolic stress. In this study, we present a ratiometric fluorescent biosensor for lactic acid detection, utilizing a fluorescence quenching mechanism. The sensor comprises a hydrogen peroxide (H₂O₂)-sensing unit, based on photoluminescent core-shell cadmium telluride@cadmium sulfide quantum dots (CdTe@CdS QDs), along with a reference probe covalently bound to silica microparticle (SiO₂ MPs), which acts as the substrate. Lactate oxidase (LOx) is immobilized on the microparticle surface, where it catalyzes the aerobic oxidation of l-lactate into pyruvate, generating H₂O₂ in the process. The increasing concentrations of lactate (0–30 mM) result in proportional quenching of the CdTe@CdS QDs’ photoluminescence due to H₂O₂, while the reference fluorescence emission remains stable. A lactate calibration curve has been determined using confocal laser scanning microscopy (CLSM), enabling quantitative evaluation of the sensor's ratiometric response and colorimetric shifts via image analysis. To demonstrate the versatility of this approach, we engineered two variations of the microsensor with distinct ratiometric setups, one using green- and the other red-emitting CdTe@CdS QDs, paired with either rhodamine isothiocyanate (RBITC) or 7-(diethylamino)coumarin-3-carboxylic acid (7ACC1) as the reference dyes, both co-immobilized with LOx enzyme on the microparticle surface. These innovative microsensors offer a simple yet effective tool for the quantitative detection of lactic acid, leveraging its H₂O₂-sensing capability of CdTe@CdS QDs in combination with to LOx enzyme activity.

{"title":"Ratiometric fluorescent biosensors for quantitative lactic acid detection using CdTe@CdS quantum dots and lactate oxidase","authors":"Giuliana Grasso , Riccardo Scarfiello , Francesco Colella , Valentina Onesto , Stefania Forciniti , Giuseppe Gigli , Luigi Carbone , Loretta L. del Mercato","doi":"10.1016/j.snr.2025.100303","DOIUrl":"10.1016/j.snr.2025.100303","url":null,"abstract":"<div><div>Lactic acid plays a crucial role in various physiological processes, particularly in cellular metabolism and muscle activity, as it is a key marker of anaerobic respiration and metabolic stress. In this study, we present a ratiometric fluorescent biosensor for lactic acid detection, utilizing a fluorescence quenching mechanism. The sensor comprises a hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>)-sensing unit, based on photoluminescent core-shell cadmium telluride@cadmium sulfide quantum dots (CdTe@CdS QDs), along with a reference probe covalently bound to silica microparticle (SiO<sub>2</sub> MPs), which acts as the substrate. Lactate oxidase (LOx) is immobilized on the microparticle surface, where it catalyzes the aerobic oxidation of <span>l</span>-lactate into pyruvate, generating H<sub>2</sub>O<sub>2</sub> in the process. The increasing concentrations of lactate (0–30 mM) result in proportional quenching of the CdTe@CdS QDs’ photoluminescence due to H<sub>2</sub>O<sub>2</sub>, while the reference fluorescence emission remains stable. A lactate calibration curve has been determined using confocal laser scanning microscopy (CLSM), enabling quantitative evaluation of the sensor's ratiometric response and colorimetric shifts via image analysis. To demonstrate the versatility of this approach, we engineered two variations of the microsensor with distinct ratiometric setups, one using green- and the other red-emitting CdTe@CdS QDs, paired with either rhodamine isothiocyanate (RBITC) or 7-(diethylamino)coumarin-3-carboxylic acid (7ACC1) as the reference dyes, both co-immobilized with LOx enzyme on the microparticle surface. These innovative microsensors offer a simple yet effective tool for the quantitative detection of lactic acid, leveraging its H<sub>2</sub>O<sub>2</sub>-sensing capability of CdTe@CdS QDs in combination with to LOx enzyme activity.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100303"},"PeriodicalIF":6.5,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143395803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of molecular structure and plasticization of PVC membranes in the response of solid-state ion-selective electrodes

IF 6.5 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports

Pub Date : 2025-02-05 DOI: 10.1016/j.snr.2025.100301

Coral Salvo-Comino , Luis E. Alonso-Pastor , Clara Pérez-González , Stefano Pettinelli , Karina C. Núñez Carrero , Miguel Ángel Rodríguez-Pérez , María Luz Rodríguez-Méndez

Potentiometric sensors are essential in various industries due to their high sensitivity, simplicity, and cost-effectiveness. However, enhancing sensitivity, largely dependent on membrane diffusion, remains challenging. This study investigates the role of polyvinyl chloride (PVC) molecular weight and plasticizer type on optimizing potentiometric sensor performance for tartaric acid detection. Two PVCs with different molecular weights (HIGH and LOW) and distinct plasticizers were evaluated. Results demonstrated that membranes plasticized with dibutyl sebacate, a linear plasticizer, exhibited high sensitivity (0.0239 mV/pX) and repeatability, achieving a lower limit of detection (LOD = 4.31 × 10⁻⁵ M) compared to those with a bulky plasticizer. Specifically, HIGH PVC combined with a linear plasticizer provided saturation-free measurements, ensuring greater accuracy in tartaric acid quantification. This research underscores the impact of plasticizer selection on sensor optimization, enhancing accuracy and reliability in food quality control. By establishing the relationship between PVC structure and plasticizer type, this study contributes to advancing sensor technology for improved food safety and quality assessment.

{"title":"Impact of molecular structure and plasticization of PVC membranes in the response of solid-state ion-selective electrodes","authors":"Coral Salvo-Comino , Luis E. Alonso-Pastor , Clara Pérez-González , Stefano Pettinelli , Karina C. Núñez Carrero , Miguel Ángel Rodríguez-Pérez , María Luz Rodríguez-Méndez","doi":"10.1016/j.snr.2025.100301","DOIUrl":"10.1016/j.snr.2025.100301","url":null,"abstract":"<div><div>Potentiometric sensors are essential in various industries due to their high sensitivity, simplicity, and cost-effectiveness. However, enhancing sensitivity, largely dependent on membrane diffusion, remains challenging. This study investigates the role of polyvinyl chloride (PVC) molecular weight and plasticizer type on optimizing potentiometric sensor performance for tartaric acid detection. Two PVCs with different molecular weights (HIGH and LOW) and distinct plasticizers were evaluated. Results demonstrated that membranes plasticized with dibutyl sebacate, a linear plasticizer, exhibited high sensitivity (0.0239 mV/pX) and repeatability, achieving a lower limit of detection (LOD = 4.31 × 10⁻⁵ M) compared to those with a bulky plasticizer. Specifically, HIGH PVC combined with a linear plasticizer provided saturation-free measurements, ensuring greater accuracy in tartaric acid quantification. This research underscores the impact of plasticizer selection on sensor optimization, enhancing accuracy and reliability in food quality control. By establishing the relationship between PVC structure and plasticizer type, this study contributes to advancing sensor technology for improved food safety and quality assessment.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100301"},"PeriodicalIF":6.5,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143334065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecularly imprinted polypyrrole-modified screen-printed electrode: Toward a sensor for furfural derivatives determination

IF 6.5 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports

Pub Date : 2025-02-04 DOI: 10.1016/j.snr.2025.100299

Daniele Merli , Alessandra Cutaia , Sofia Prosperi , Camilla Zanoni , Giancarla Alberti

The increasing interest in determining furfural derivatives in food and beverages using low-cost and portable devices has driven our attention to the development of the here proposed voltammetric sensor. It is based on a screen-printed electrochemical cell whose graphite-ink working electrode is surface modified with electropolymerized molecularly imprinted polypyrrole (e-MIP), using 5-(hydroxymethyl)furan-2-carbaldehyde (HMF) as the template. Square wave voltammetry (SWV) is the selected analytical technique, exploiting the irreversible reduction peak of the carbonyl group at -1.7 V vs. Ag/AgCl pseudo reference electrode in 0.1 M phosphate buffer (PBS) at pH 8. The presence of the e-MIP on the working electrode surface enhances the sensitivity by lowering the detection limit of about one order of magnitude if compared with the bare electrode (LOD bare 93 µM; e-MIP 7 µM). The developed sensor is selective for the whole class of furfural-like compounds, allowing the determination of the total furanic aldehydes content. The sensor was applied to the analysis of synthetic solutions and real beverages, such as expresso coffee, obtaining good recovery percentages.

{"title":"Molecularly imprinted polypyrrole-modified screen-printed electrode: Toward a sensor for furfural derivatives determination","authors":"Daniele Merli , Alessandra Cutaia , Sofia Prosperi , Camilla Zanoni , Giancarla Alberti","doi":"10.1016/j.snr.2025.100299","DOIUrl":"10.1016/j.snr.2025.100299","url":null,"abstract":"<div><div>The increasing interest in determining furfural derivatives in food and beverages using low-cost and portable devices has driven our attention to the development of the here proposed voltammetric sensor. It is based on a screen-printed electrochemical cell whose graphite-ink working electrode is surface modified with electropolymerized molecularly imprinted polypyrrole (e-MIP), using 5-(hydroxymethyl)furan-2-carbaldehyde (HMF) as the template. Square wave voltammetry (SWV) is the selected analytical technique, exploiting the irreversible reduction peak of the carbonyl group at -1.7 V vs. Ag/AgCl pseudo reference electrode in 0.1 M phosphate buffer (PBS) at pH 8. The presence of the e-MIP on the working electrode surface enhances the sensitivity by lowering the detection limit of about one order of magnitude if compared with the bare electrode (LOD bare 93 µM; e-MIP 7 µM). The developed sensor is selective for the whole class of furfural-like compounds, allowing the determination of the total furanic aldehydes content. The sensor was applied to the analysis of synthetic solutions and real beverages, such as expresso coffee, obtaining good recovery percentages.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100299"},"PeriodicalIF":6.5,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143334063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acoustic bubbles: Inducing cell interactions via trapping, patterning, and removal

IF 6.5 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports

Pub Date : 2025-02-01 DOI: 10.1016/j.snr.2025.100294

Jonathan Faulkner , Mengren Wu , Madelyn Wicker , Yuan Gao

Studying cell-cell interactions is crucial for understanding biological processes and advancing drug development. Recent advancements in microfluidic technology allow cell-cell interaction experiments to be performed on-chip, enabling the study of specific interactions within a highly defined microenvironment. Acoustic bubbles, tiny bubbles excited by an external acoustic source, are clean, biocompatible, and effective tools for manipulating fluids and particles at the microscale. This research presents a novel microfluidic approach using acoustic bubbles to study cell-cell interactions. It focuses on generating, controlling, and removing acoustic bubbles within a microfluidic device to manipulate particles and cells and induce their interaction. In this work, a microbubble is passively generated to separate the channels containing two different types of particles and cells. Under the control of an acoustic field, particles and cells are trapped on either side of the microbubble. The microbubble is then removed to enable interaction between the trapped particles and cells. The impact of channel geometry on bubble stability is quantitatively investigated to optimize the device's performance. Additionally, the successful demonstration of on-chip cell interactions highlights the rapid and straightforward operation of this method. This innovative approach holds great potential for studying specific cell interactions to gain biological insights into disease progression and serves as an effective tool for drug testing.

{"title":"Acoustic bubbles: Inducing cell interactions via trapping, patterning, and removal","authors":"Jonathan Faulkner , Mengren Wu , Madelyn Wicker , Yuan Gao","doi":"10.1016/j.snr.2025.100294","DOIUrl":"10.1016/j.snr.2025.100294","url":null,"abstract":"<div><div>Studying cell-cell interactions is crucial for understanding biological processes and advancing drug development. Recent advancements in microfluidic technology allow cell-cell interaction experiments to be performed on-chip, enabling the study of specific interactions within a highly defined microenvironment. Acoustic bubbles, tiny bubbles excited by an external acoustic source, are clean, biocompatible, and effective tools for manipulating fluids and particles at the microscale. This research presents a novel microfluidic approach using acoustic bubbles to study cell-cell interactions. It focuses on generating, controlling, and removing acoustic bubbles within a microfluidic device to manipulate particles and cells and induce their interaction. In this work, a microbubble is passively generated to separate the channels containing two different types of particles and cells. Under the control of an acoustic field, particles and cells are trapped on either side of the microbubble. The microbubble is then removed to enable interaction between the trapped particles and cells. The impact of channel geometry on bubble stability is quantitatively investigated to optimize the device's performance. Additionally, the successful demonstration of on-chip cell interactions highlights the rapid and straightforward operation of this method. This innovative approach holds great potential for studying specific cell interactions to gain biological insights into disease progression and serves as an effective tool for drug testing.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100294"},"PeriodicalIF":6.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143158766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-sensitivity colorimetric sensor based on oxidase-like Mn3O4 nanozyme for Cys detection

IF 6.5 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports

Pub Date : 2025-02-01 DOI: 10.1016/j.snr.2025.100296

Junting Liang , Dihan Duan , Lubing Sun , Jinming Li , Mengke Wang , Ziyi Chang , Rick F. Thorne , Chuanliang Chen , Demin Duan

Cysteine (Cys) is essential for numerous physiological processes and play a vital role in disease diagnostics, underscoring the necessity for effective and sensitive detection techniques for Cys. In this research, we introduce an ultrasensitive colorimetric approach based on oxidase-like Mn₃O₄ nanozymes. The nanozymes were synthesized through a straightforward wet chemical method and displayed a flower-like globular morphology, showing remarkable oxidase-like catalytic performance with a low K_m value of 0.1896 mM and a high V_max of 2.8 × 10⁻⁷ M s⁻¹. By leveraging the outstanding catalytic activities of Mn₃O₄ nanozymes and optimizing the TMB concentration, we developed a novel and sensitive colorimetric sensor for Cys detection. Experimental results indicated a detection range for Cys between 0.5 and 45 μM, with a lower limit of detection (LOD) of 0.02636 μM. This established method for detecting Cys reveals significant promise for the advancement of effective sensing systems.

{"title":"High-sensitivity colorimetric sensor based on oxidase-like Mn3O4 nanozyme for Cys detection","authors":"Junting Liang , Dihan Duan , Lubing Sun , Jinming Li , Mengke Wang , Ziyi Chang , Rick F. Thorne , Chuanliang Chen , Demin Duan","doi":"10.1016/j.snr.2025.100296","DOIUrl":"10.1016/j.snr.2025.100296","url":null,"abstract":"<div><div>Cysteine (Cys) is essential for numerous physiological processes and play a vital role in disease diagnostics, underscoring the necessity for effective and sensitive detection techniques for Cys. In this research, we introduce an ultrasensitive colorimetric approach based on oxidase-like Mn<sub>3</sub>O<sub>4</sub> nanozymes. The nanozymes were synthesized through a straightforward wet chemical method and displayed a flower-like globular morphology, showing remarkable oxidase-like catalytic performance with a low K<sub>m</sub> value of 0.1896 mM and a high V<sub>max</sub> of 2.8 × 10⁻⁷ M s⁻¹. By leveraging the outstanding catalytic activities of Mn<sub>3</sub>O<sub>4</sub> nanozymes and optimizing the TMB concentration, we developed a novel and sensitive colorimetric sensor for Cys detection. Experimental results indicated a detection range for Cys between 0.5 and 45 μM, with a lower limit of detection (LOD) of 0.02636 μM. This established method for detecting Cys reveals significant promise for the advancement of effective sensing systems.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100296"},"PeriodicalIF":6.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143334066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-frequency electrochemical impedance measurements of self-assembled monolayer formation using CMOS-based nanocapacitor arrays

IF 6.5 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports

Pub Date : 2025-02-01 DOI: 10.1016/j.snr.2025.100293

Selvaraj Chinnathambi , Mohammad Saghafi , Suryasnata Tripathy , Frans P. Widdershoven , Serge G. Lemay

Electrochemical impedance spectroscopy is mostly sensitive to the state of the electrode surface at low frequencies, where the electrical properties of the surrounding electrolyte do not yet dominate the response. The available frequency range increases linearly with the inverse electrode size, such that miniaturized electrodes offer the ability to probe the surface at higher frequencies. Measuring the AC response of miniaturized electrodes is however highly challenging due to the parasitic capacitance of the interconnects. Here we employ nanoelectrodes integrated with readout circuitry on the same complementary metal-oxide-semiconductor (CMOS) chip to conduct measurements over a frequency range from 1.6 MHz to 50 MHz. We report the ability to probe the formation of self-assembled monolayers at frequency above 10 MHz in phosphate-buffered saline solution.

引用次数: 0

SARS-COV-2 viral RNA detection through oligonucleotide-capped nanoporous anodic alumina supports.

IF 6.5 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports

Pub Date : 2025-02-01 DOI: 10.1016/j.snr.2025.100298

Alba López-Palacios , María Nieves Aranda , Isabel Caballos , Andy Hernández-Montoto , Eva Calabuig , María Dolores Gómez-Ruiz , María Ángeles Tormo-Mas , Javier Pemán , Félix Sancenón , Ramón Martínez-Máñez , Elena Aznar , Estela Climent

We describe herein a sensor containing nanoporous anodic alumina (NAA) as sensing platform to identify SARS-COV-2 RNA using a gating mechanism. The system is based on NAA that contains a fluorescent dye (Rhodamine B; RhB) and is capped with an oligonucleotide sequence that hybridize specifically a region of SARS-CoV-2 genome. In the presence of RNA from SARS-COV-2 virus, the oligonucleotide of the surface is displaced, uncapping the pores, and producing a delivery of RhB. The detection of the virus is achieved measuring the fluorescence of the fluorophore. The nanosensor demonstrates to be highly sensitive and selective in aqueous buffers and in biological media, having a limit of detection (LOD) of 50 ± 30 copies mL^-1 of SARS-CoV-2 RNA extracted from patients. Moreover, preliminary results using 18 real nasopharyngeal swab samples indicate the potential of the system to differentiate between infected and non-infected patients. Compared to the conventional RT-PCR method, in our system there is no need for sample pretreatment or RNA isolation, providing diagnostic outcomes within 60 mins while maintaining a high level of reliability.

{"title":"SARS-COV-2 viral RNA detection through oligonucleotide-capped nanoporous anodic alumina supports.","authors":"Alba López-Palacios , María Nieves Aranda , Isabel Caballos , Andy Hernández-Montoto , Eva Calabuig , María Dolores Gómez-Ruiz , María Ángeles Tormo-Mas , Javier Pemán , Félix Sancenón , Ramón Martínez-Máñez , Elena Aznar , Estela Climent","doi":"10.1016/j.snr.2025.100298","DOIUrl":"10.1016/j.snr.2025.100298","url":null,"abstract":"<div><div>We describe herein a sensor containing nanoporous anodic alumina (NAA) as sensing platform to identify SARS-COV-2 RNA using a gating mechanism. The system is based on NAA that contains a fluorescent dye (Rhodamine B; RhB) and is capped with an oligonucleotide sequence that hybridize specifically a region of SARS-CoV-2 genome. In the presence of RNA from SARS-COV-2 virus, the oligonucleotide of the surface is displaced, uncapping the pores, and producing a delivery of RhB. The detection of the virus is achieved measuring the fluorescence of the fluorophore. The nanosensor demonstrates to be highly sensitive and selective in aqueous buffers and in biological media, having a limit of detection (LOD) of 50 ± 30 copies mL<sup>-1</sup> of SARS-CoV-2 RNA extracted from patients. Moreover, preliminary results using 18 real nasopharyngeal swab samples indicate the potential of the system to differentiate between infected and non-infected patients. Compared to the conventional RT-PCR method, in our system there is no need for sample pretreatment or RNA isolation, providing diagnostic outcomes within 60 mins while maintaining a high level of reliability.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100298"},"PeriodicalIF":6.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143348603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The design of paper-based electroanalytical microfluidic device coupled with post-synthesized molecularly imprinted polymers (rGO/Au@Ag2S/PANI/polyacrylamide) for the detection of streptomycin

IF 6.5 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports

Pub Date : 2025-01-31 DOI: 10.1016/j.snr.2025.100297

Mina Adampourezare , Karim Asadpour-Zeynali , Miguel de la Guardia , Jafar Ezzati Nazhad Dolatabadi

A highly sensitive three-electrode paper-based microfluidic device has been developed for determinate of streptomycin. Ag/GQD/chitosan nano ink was used to construct reference and counter electrodes and Au@Ag₂S/GQD/chitosan nano ink was used to construct working electrode. rGO/Au@Ag₂S/anline nano-sheets were encapsulated with acrylamide as a functional monomer to create novel nano-sized molecular imprinted polymer (MIP) and was used for the modification of electrode working.

Paper substrate was modified for forming a hydrophobic region around the hydrophilic region on the filter paper and were suitable for the evaluation of target molecules in aqueous environment. All steps of making the MIP and non-imprinted polymer (NIP) sensors are illustrated by FE-SEM, Energy-dispersive X-ray spectroscopy (EDS), and FT-IR. CV and DPV techniques were applied for the measurement of the streptomycin. The fabricated device showed high sensitivity toward streptomycin, which was due to synergistic effects between catalytic properties, electrical conductivity of rGO/Au@Ag₂S/anline /Polyacrylamide, and considerably increased numbers of imprinted sites. Using these techniques, streptomycin was determined in the linear range of 1pM to 1 μM with a limit of detection of 0.9 × 10⁻¹² M (Ip (μA) = -2.075x + 31.575, R²= 0.9983). The device provided good cyclic stability (RSD < 0.05), high reproducibility and selectivity (RSD < 0.05 obtained from five calibration plots) with a good repeatability (for both milk and serum samples, RSD < 0.05). Finally, it should be noted that this device showed a good analytical performance for the detection of streptomycin in milk and serum samples.

{"title":"The design of paper-based electroanalytical microfluidic device coupled with post-synthesized molecularly imprinted polymers (rGO/Au@Ag2S/PANI/polyacrylamide) for the detection of streptomycin","authors":"Mina Adampourezare , Karim Asadpour-Zeynali , Miguel de la Guardia , Jafar Ezzati Nazhad Dolatabadi","doi":"10.1016/j.snr.2025.100297","DOIUrl":"10.1016/j.snr.2025.100297","url":null,"abstract":"<div><div>A highly sensitive three-electrode paper-based microfluidic device has been developed for determinate of streptomycin. Ag/GQD/chitosan nano ink was used to construct reference and counter electrodes and Au@Ag<sub>2</sub>S/GQD/chitosan nano ink was used to construct working electrode. rGO/Au@Ag<sub>2</sub>S/anline nano-sheets were encapsulated with acrylamide as a functional monomer to create novel nano-sized molecular imprinted polymer (MIP) and was used for the modification of electrode working.</div><div>Paper substrate was modified for forming a hydrophobic region around the hydrophilic region on the filter paper and were suitable for the evaluation of target molecules in aqueous environment. All steps of making the MIP and non-imprinted polymer (NIP) sensors are illustrated by FE-SEM, Energy-dispersive X-ray spectroscopy (EDS), and FT-IR. CV and DPV techniques were applied for the measurement of the streptomycin. The fabricated device showed high sensitivity toward streptomycin, which was due to synergistic effects between catalytic properties, electrical conductivity of rGO/Au@Ag<sub>2</sub>S/anline /Polyacrylamide, and considerably increased numbers of imprinted sites. Using these techniques, streptomycin was determined in the linear range of 1pM to 1 μM with a limit of detection of 0.9 × 10<sup>−12</sup> M (Ip (μA) = -2.075x + 31.575, R<sup>2</sup>= 0.9983). The device provided good cyclic stability (RSD < 0.05), high reproducibility and selectivity (RSD < 0.05 obtained from five calibration plots) with a good repeatability (for both milk and serum samples, RSD < 0.05). Finally, it should be noted that this device showed a good analytical performance for the detection of streptomycin in milk and serum samples.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100297"},"PeriodicalIF":6.5,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143348597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanotechnology-driven electrochemical neurotransmitter sensing as a fundamental approach towards improving diagnostics and therapeutics: A review

IF 6.5 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports

Pub Date : 2025-01-30 DOI: 10.1016/j.snr.2025.100292

Mehr Un Nisa , Asim Yaqub , Muhammad Hashim Khan , Fatima Yaseen , Shahaab Jilani , Huma Ajab , Noor S. Shah , Abdulaziz Al-Anazi

Neurotransmitters (NTs) are endogenous chemical messengers that have a wide range of key roles throughout the nervous system. It is an important signal medium of neural transfers and equally influence physiological conditions. While, abnormal levels are linked with psychotic, physical, and neurodegenerative disorders such as ADHD, autism and tourette syndrome. Therefore, their accurate and sensitive detection is of great importance. Traditional methods of NTs detection are limited to complex sample processing, high cost, and real-time diagnostics. However, an electrochemical sensing has been extensively utilized in the past few years for NTs detection, which shows better sensitivity, selectivity, and capacity for operation in complicated biological environments, constitutes a promising approach. In this review, the most successful and favorable electrochemical sensors based on innovations in electrode materials and nanostructured surfaces having substantial improvement in the analytical performances and overcoming challenges followed by future prospects in NTs detection have been reported. This review additionally looks at the progress accomplished in biosensors for neurotransmitter evaluations in microfluidic lab-on-a-chip systems, smart wearable devices and other advanced technologies for the goal of real-time, portable diagnosis in neuroscience and clinics. These advances are paving the way for more effective applications of electrochemical sensing in neuroscience and clinical diagnostics.

{"title":"Nanotechnology-driven electrochemical neurotransmitter sensing as a fundamental approach towards improving diagnostics and therapeutics: A review","authors":"Mehr Un Nisa , Asim Yaqub , Muhammad Hashim Khan , Fatima Yaseen , Shahaab Jilani , Huma Ajab , Noor S. Shah , Abdulaziz Al-Anazi","doi":"10.1016/j.snr.2025.100292","DOIUrl":"10.1016/j.snr.2025.100292","url":null,"abstract":"<div><div>Neurotransmitters (NTs) are endogenous chemical messengers that have a wide range of key roles throughout the nervous system. It is an important signal medium of neural transfers and equally influence physiological conditions. While, abnormal levels are linked with psychotic, physical, and neurodegenerative disorders such as ADHD, autism and tourette syndrome. Therefore, their accurate and sensitive detection is of great importance. Traditional methods of NTs detection are limited to complex sample processing, high cost, and real-time diagnostics. However, an electrochemical sensing has been extensively utilized in the past few years for NTs detection, which shows better sensitivity, selectivity, and capacity for operation in complicated biological environments, constitutes a promising approach. In this review, the most successful and favorable electrochemical sensors based on innovations in electrode materials and nanostructured surfaces having substantial improvement in the analytical performances and overcoming challenges followed by future prospects in NTs detection have been reported. This review additionally looks at the progress accomplished in biosensors for neurotransmitter evaluations in microfluidic lab-on-a-chip systems, smart wearable devices and other advanced technologies for the goal of real-time, portable diagnosis in neuroscience and clinics. These advances are paving the way for more effective applications of electrochemical sensing in neuroscience and clinical diagnostics.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100292"},"PeriodicalIF":6.5,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143334062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

下一页尾页

类型

全部化学•材料生命科学医学物理工程技术环境•农林材料科学地球科学法学管理学化学环境科学与生态学计算机科学教育学经济学农林科学人文科学生物学数学物理与天体物理心理学综合性期刊其他工业工程理学历史学农学文学信息工程

数据库

全部 ACS Publications Elsevier ieeexplore Springer The Royal Society of Chemistry Wiley

期刊

Sensors and Actuators Reports

全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.

﹀