Sensors and Actuators Reports最新文献

A point-of-care test for miR-129–5p detection at sub-atto molar concentrations exploiting plasmonic pollen probes combined with complementary DNA 利用等离子体花粉探针结合互补DNA进行亚atatmolar浓度miR-129-5p检测的即时检测

IF 7.6 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports

Pub Date : 2025-12-08 DOI: 10.1016/j.snr.2025.100417

Laura Pasquardini , Rosalba Pitruzzella , Francesco Arcadio , Mafalda Rizzuti , Valentina Melzi , Francesca Sironi , Lia Vanzetti , Andrea Chiappini , Chiara Perri , Linda Ottoboni , Nunzio Cennamo , Stefania Corti , Luigi Zeni

MicroRNAs represent an emerging class of biomarkers that can be correlated with pathological situations. Precise and rapid detection of these biomarkers via Point-of-Care Tests (POCT) can be crucial for early disease identification or monitoring of medical treatments. In this work, an ultra-highly sensitive biosensing strategy for POCT, based on hybrid plasmonic phenomena in pollen-based nanostructures covered by gold, combined with a specific bioreceptor layer, is presented. The miRNA detection is carried out in just 15 min by hybridization, without any target amplification or complex enhancement, simply by diluting the sample in a suitable buffer. As a proof of concept, miR-129–5p is selected as possibly correlated with Atrophic Lateral Sclerosis (ALS) and its detection is performed in buffer and diluted human serum, achieving a limit of detection (LOD) of approximately 100 zM. The proposed POCT is characterized via X-ray Photoelectron Spectrocopy (XPS) and microRAMAN, dose-response curves, selectivity tests, and test in real samples. Moreover, the detection of miR-129–5p in ALS serum patients has been compared with qRT-PCR to highlight the impressive performances obtained with the POCT, just diluting the sample, without any additional treatment. These experimental results will open a new class of POCT with ultra-high sensitivity, ultra-high specificity, and ultra-fast response, all exploiting simple equipment and nanoplasmonic biochips. Specifically, considering the advantages of using diluted real samples and the achieved detection range, the proposed sensing approach could be useful for enhancing cancer diagnosis by detecting mature circulating miRNA in various body fluids.

MicroRNAs代表了一类新兴的生物标志物，可以与病理情况相关。通过即时检测（POCT）精确、快速地检测这些生物标志物对于早期疾病识别或医学治疗监测至关重要。在这项工作中，提出了一种超高灵敏度的POCT生物传感策略，该策略基于金覆盖的花粉基纳米结构中的杂化等离子体现象，并结合特定的生物受体层。通过杂交在15分钟内进行miRNA检测，无需任何目标扩增或复杂增强，只需将样品稀释在合适的缓冲液中。作为概念证明，miR-129-5p被选择为可能与萎缩性侧索硬化症（ALS）相关，并在缓冲液和稀释的人血清中进行检测，检测限（LOD）约为100 zM。通过x射线光电子能谱（XPS）和microroraman，剂量-响应曲线，选择性测试和实际样品测试对所提出的POCT进行了表征。此外，我们还将检测ALS患者血清中的miR-129-5p与qRT-PCR进行了比较，以突出POCT所获得的令人印象深刻的性能，只需稀释样品，无需任何额外处理。这些实验结果将开启一类新的POCT，具有超高灵敏度、超高特异性和超快速响应，所有这些都利用简单的设备和纳米等离子体生物芯片。具体来说，考虑到使用稀释真实样本的优势以及所达到的检测范围，本文提出的传感方法可以通过检测各种体液中的成熟循环miRNA来增强癌症诊断。

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引用次数: 0

Polymer nanofiber-based electrodes for tyrosinase detection in melanoma diagnosis 基于聚合物纳米纤维的酪氨酸酶检测电极在黑色素瘤诊断中的应用

IF 7.6 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports

Pub Date : 2025-12-08 DOI: 10.1016/j.snr.2025.100416

Nazanin Poursharifi , Dariush Semnani , Hossein Fashandi , Ali A. Ensafi , Alireza Sanati , Onur Parlak

The rising global incidence of melanoma and its high mortality rates due to late diagnosis demand rapid, sensitive, and non-invasive diagnostic tools. Here, we introduce a disposable electrochemical biosensor that exploits the synergistic properties of electrospun polyamide 6 (PA6) and conductive polypyrrole (PPy) nanofibers for detecting tyrosinase (Tyr). The biosensor integrates the high surface area and tunable morphology of PA6 nanofibers (random and aligned) with highly conductive PPy, fabricated via electrospinning, and polymerization onto indium tin oxide (ITO) electrodes to ensure selective Tyr binding. Electrochemical testing in synthetic interstitial fluid (ISF) and human serum reveals a linear response to Tyr (0.1–0.9 mg/mL), with the aligned nanofiber configuration achieving high limits of detection (LOD) of 0.03 mg/mL (ISF) and 0.029 mg/mL (serum), alongside sensitivities of 95.97 and 76.60 μA·mg⁻¹·mL·cm⁻², respectively. The concentration-dependent current response highlights the biosensor’s high sensitivity, stability, and biocompatibility, proving its potential for non-invasive, early-stage melanoma detection in complex biological environments. This work paves the way for scalable, cost-effective melanoma screening, with future studies aimed at clinical validation and integration into point-of-care devices to improve patient outcomes globally.

全球黑色素瘤发病率的上升及其因诊断晚而导致的高死亡率需要快速、敏感和非侵入性的诊断工具。在这里，我们介绍了一种利用静电纺聚酰胺6 （PA6）和导电聚吡咯（PPy）纳米纤维的协同特性来检测酪氨酸酶（Tyr）的一次性电化学生物传感器。该生物传感器将PA6纳米纤维（随机排列）的高表面积和可调形态与高导电性的聚吡啶（PPy）结合在一起，通过静电纺丝和聚合在氧化铟锡（ITO）电极上，以确保选择性地结合Tyr。在合成间质液（ISF）和人血清中的电化学测试显示，Tyr对0.1-0.9 mg/mL呈线性反应，排列的纳米纤维结构的高检出限（LOD）分别为0.03 mg/mL （ISF）和0.029 mg/mL（血清），灵敏度分别为95.97和76.60 μA·mg·mg⁻¹·mL·cm⁻²。浓度依赖性电流响应突出了生物传感器的高灵敏度、稳定性和生物相容性，证明了其在复杂生物环境中非侵入性、早期黑色素瘤检测的潜力。这项工作为可扩展的、具有成本效益的黑色素瘤筛查铺平了道路，未来的研究旨在临床验证和整合到护理点设备中，以改善全球患者的预后。

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引用次数: 0

Genosensors as a powerful and next generation diagnostic approach of bacterial infections: From the current methods and their challenges to clinical application of genosensors 基因传感器作为下一代细菌感染的强大诊断手段：从目前的方法及其挑战到基因传感器的临床应用

IF 7.6 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports

Pub Date : 2025-12-07 DOI: 10.1016/j.snr.2025.100415

Mehdi Bakht , Saeideh Gholamzadeh Khoei , Nima Aria , Sara Rahimi , Hamid Sadeghi , Samira Sabzi , Abouzar Babaei

Rapid and highly specific detection of bacterial pathogens remains a major challenge in clinical diagnostics, food safety, and environmental monitoring. While conventional culture and PCR-based assays provide reliable results, their reliance on laboratory infrastructure and long processing times limits their applicability for point of care testing. Genosensors biosensing platforms that detect bacterial nucleic acids through controlled probe immobilization and hybridization offer a promising alternative. This review critically examines recent advances in biorecognition elements (ssDNA, PNA, LNA), probe immobilization chemistries (thiol-gold SAMs, EDC/NHS coupling, click based strategies, and affinity systems), and diverse transduction formats including electrochemical, optical, and emerging hybrid approaches. Key developments enabling enhanced sensitivity, reduced nonspecific interactions, and improved performance in complex matrices are highlighted. Unlike previous pathogen by pathogen descriptive surveys, this review provides a comparative, mechanism focused analysis that identifies the strengths and limitations of each sensing strategy. Major translational barriers including surface fouling, limited reproducibility, lack of standardized fabrication workflows, and insufficient preclinical validation are discussed alongside emerging solutions such as antifouling coatings, microfluidic preprocessing, and standardized probe-surface assemblies. Overall, genosensors are positioned to evolve from laboratory scale prototypes into clinically relevant, multiplexed diagnostic tools. Continued progress toward robust sample-to-answer integration, regulatory-aligned validation, and scalable manufacturing will be essential for their widespread deployment in real world applications.

快速和高度特异性的细菌性病原体检测仍然是临床诊断、食品安全和环境监测的主要挑战。虽然传统的培养和基于pcr的检测方法提供了可靠的结果，但它们对实验室基础设施的依赖和较长的处理时间限制了它们在护理点检测中的适用性。通过控制探针固定和杂交来检测细菌核酸的基因传感器生物传感平台提供了一个有前途的选择。本文综述了生物识别元件（ssDNA， PNA， LNA），探针固定化化学（硫金SAMs， EDC/NHS耦合，基于点击的策略和亲和系统）以及各种转导格式（包括电化学，光学和新兴混合方法）的最新进展。重点介绍了在复杂矩阵中增强灵敏度、减少非特异性相互作用和改进性能的关键进展。与以往的病原体描述性调查不同，本综述提供了一种比较的、以机制为重点的分析，确定了每种感知策略的优势和局限性。主要的转化障碍包括表面污染、有限的可重复性、缺乏标准化的制造工作流程和临床前验证不足，以及新兴的解决方案，如防污涂层、微流体预处理和标准化探针表面组件。总的来说，基因传感器的定位是从实验室规模的原型发展到临床相关的多路诊断工具。继续朝着强大的样本到答案集成、符合法规的验证和可扩展制造的方向发展，将是它们在现实世界应用中广泛部署的必要条件。

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引用次数: 0

Integrated SERS-magnetic capture platform for multiplex detection of early tubular injury biomarkers in diabetic kidney disease progression 集成sers磁捕获平台用于多重检测糖尿病肾病进展中的早期肾小管损伤生物标志物

IF 7.6 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports

Pub Date : 2025-12-02 DOI: 10.1016/j.snr.2025.100412

Wendi Cao , Sirui Yang , Zhenhua Zhao , Xuelin Chen , Tenglong Liu , Xinran Yang , Leshan Cai , Xiaozhe Lin , Xia Zhou , Qiaoxin Zhang

Early detection of tubular injury biomarkers in diabetic kidney disease (DKD) is critical for timely intervention. This study presents an innovative magnetic separation-assisted surface-enhanced Raman spectroscopy (SERS) immunoassay for ultrasensitive, multiplex detection of three tubular injury biomarkers: retinol-binding protein 4 (RBP4), neutrophil gelatinase-associated lipocalin (NGAL), and kidney injury molecule-1 (KIM1). The platform combines three key technological innovations: (1) Raman internal standard (4-mercaptobenzoic acid)-embedded Fe₃O₄-AuAg core-satellite nanoparticles with optimized plasmonic-magnetic properties; (2) Spectrally distinct Raman probes using Au nanorods functionalized with three reporter molecules (2-naphthalenethiol, 2-mercaptopyridine, 4-nitrobenzenethiol); and (3) An automated magnetic separation workflow greatly reduces sample enrichment time. The assay demonstrated unprecedented sensitivity with limits of detection of 10 fg/mL (RBP4), 10 fg/mL (NGAL), and 1 pg/mL (KIM1) in PBS solution, surpassing traditional common methods by 3-4 orders of magnitude. Clinical validation with 24 samples showed excellent correlation with the conventional Luminex assay method (Pearson's r=0.96-0.99), and the coefficient of variation was less than 9.28%, with Bland-Altman analysis confirming 95% agreement. This integrated SERS-magnetic approach enables simultaneous, high-sensitivity detection of multiplex tubular injury biomarkers, offering significant potential for early DKD diagnosis and progression monitoring. The demonstrated analytical performance and clinical concordance position this technology as a promising tool for precision nephrology.

糖尿病肾病（DKD）小管损伤生物标志物的早期检测对于及时干预至关重要。本研究提出了一种创新的磁分离辅助表面增强拉曼光谱（SERS）免疫分析法，用于超灵敏、多种检测三种肾小管损伤生物标志物：视黄醇结合蛋白4 （RBP4）、中性粒细胞明胶酶相关脂钙蛋白（NGAL）和肾损伤分子-1 （KIM1）。该平台结合了三个关键技术创新：(1)拉曼内标（4-巯基苯甲酸）-嵌入Fe3O4-AuAg核心-卫星纳米粒子，优化了等离子体磁性能；(2)利用三种报告分子（2-萘硫醇、2-巯基吡啶、4-硝基苯硫醇）功能化的金纳米棒进行光谱区分的拉曼探针；(3)自动化磁选工作流程大大缩短了样品富集时间。该方法具有前所未有的灵敏度，在PBS溶液中检测限为10 fg/mL (RBP4), 10 fg/mL （NGAL）和1 pg/mL (KIM1)，超过传统常用方法3-4个数量级。24份样品的临床验证与传统的Luminex检测方法具有良好的相关性（Pearson’s r=0.96 ~ 0.99），变异系数小于9.28%，Bland-Altman分析的一致性为95%。这种集成的sers磁性方法可以同时、高灵敏度地检测多种管状损伤生物标志物，为早期DKD诊断和进展监测提供了巨大的潜力。所展示的分析性能和临床一致性使该技术成为精确肾病学的一个有前途的工具。

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引用次数: 0

Dual nanoparticle-enhanced biophotonic fiber sensor for highly sensitive and selective detection of pseudomonas aeruginosa in food safety applications 双纳米粒子增强生物光子光纤传感器在铜绿假单胞菌检测中的高灵敏度和选择性应用

IF 7.6 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports

Pub Date : 2025-12-01 DOI: 10.1016/j.snr.2025.100409

Qi Zhang , Ragini Singh , Rui Min , Ben Niu , Wilfried Blanc , Bingyuan Zhang , Santosh Kumar

Pseudomonas aeruginosa (P. aeruginosa), as a foodborne pathogen, is often underestimated in the field of food safety. In light of this, the development of a sensor for detecting P. aeruginosa that possesses both high sensitivity and high selectivity is of great significance for food safety monitoring and the medical health field. Based on the localized surface plasmon resonance (LSPR) effect, this study designs and constructs a novel biophotonic fiber sensor using gold nanoparticles (AuNPs) and platinum nanoparticles (PtNPs) with significant LSPR effects, aiming to efficiently detect P. aeruginosa. The core of the sensor is to fix specific antibodies on the surface of nanoparticles and achieve detection by monitoring the LSPR spectral shift triggered by the binding of bacteria to antibodies. The sensor demonstrates a low detection limit of 2.6 CFU/mL, which represents a significant advantage in the current technological context. Furthermore, this study enhances the selectivity of the sensor by functionalizing antibodies to meet the specific requirements for screening P. aeruginosa in food. To comprehensively evaluate the performance of the sensor, we tested its stability, repeatability, and selectivity, with results showing excellent performance. The formation of biofilm by P. aeruginosa is one of its major virulence factors, and our sensor can also accurately detect the presence of biofilm. Further application of the sensor in tests simulating real-world environments indicates that the sensor has the potential for practical application. These advantages not only confirm the high efficiency of the proposed sensor in accurately detecting P. aeruginosa but also highlight its significant application prospects in the field of food safety detection.

铜绿假单胞菌（P. aeruginosa）作为一种食源性致病菌，在食品安全领域往往被低估。鉴于此，开发一种既具有高灵敏度又具有高选择性的铜绿假单胞菌检测传感器，对于食品安全监测和医疗卫生领域具有重要意义。基于局部表面等离子体共振（LSPR）效应，利用具有明显LSPR效应的金纳米粒子（AuNPs）和铂纳米粒子（PtNPs）设计并构建了新型生物光子光纤传感器，旨在高效检测铜绿假单胞菌（P. aeruginosa）。该传感器的核心是将特异性抗体固定在纳米颗粒表面，通过监测细菌与抗体结合引发的LSPR光谱位移来实现检测。该传感器具有2.6 CFU/mL的低检测限，在当前的技术环境中具有显著的优势。此外，本研究通过功能化抗体来提高传感器的选择性，以满足食品中铜绿假单胞菌筛选的特定要求。为了综合评价该传感器的性能，我们对其稳定性、重复性和选择性进行了测试，结果显示其性能优异。铜绿假单胞菌形成生物膜是其主要毒力因素之一，我们的传感器也能准确检测到生物膜的存在。该传感器在模拟真实环境中的进一步应用表明，该传感器具有实际应用的潜力。这些优点不仅证实了该传感器在准确检测铜绿假单胞菌方面的高效率，也凸显了其在食品安全检测领域的重要应用前景。

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引用次数: 0

Corrigendum to “A systematic review of continuous glucose monitoring sensors: principles, core technologies and performance evaluation” [Sensors and Actuators Reports, Volume 10, December 2025, 100361] “连续血糖监测传感器的系统回顾：原理、核心技术和性能评估”的勘误表[传感器和执行器报告，第10卷，2025年12月，100361]

IF 7.6 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports

Pub Date : 2025-12-01 DOI: 10.1016/j.snr.2025.100381

Xinying Wu , Xingyu Zhao , Weiliang Chen , Qinghua Chen , Linghai Kong , Peiyao Li

引用次数: 0

RNA detection on a microfluidic platform using Thyclotides 利用Thyclotides在微流控平台上检测RNA

IF 7.6 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports

Pub Date : 2025-11-26 DOI: 10.1016/j.snr.2025.100410

Harsha Amarasekara , Riley Lehman , Paniz Rezvan Sangsari , Nicole Y. Morgan , Brian O'Farrell , Daniel H. Appella

Nucleic acid detection is commonly used to diagnose pathogenic and genetic diseases. Diagnostics based on polymerase chain reaction (PCR) are routinely used to amplify and signal the presence of a target nucleic acid, but PCR-based methods are difficult to deploy at the patient’s point-of-care and in resource-limited environments. This study reports a novel nucleic acid detection assay that utilizes chemically modified PNAs to directly detect a target RNA without enzymatic amplification. By incorporating trans-3,4-diaminotetrahydrofuran units into a PNA backbone, THF-PNAs (also called thyclotides) were designed with both enhanced binding affinity to target nucleic acids and bio-orthogonal properties that promote thyclotide-to-thyclotide binding over nucleic acid binding. Specifically, incorporation of R,R-THF monomers within the thyclotide backbone promotes a right-handed helix favoring binding to target nucleic acids. Bio-orthogonal thyclotide containing S,S-THF monomers promotes a left-handed helix, preventing their binding to natural nucleic acids. Complementary bio-orthogonal thyclotides can bind to each other in the presence of competing RNA sequences, and these unique properties were used in combination with gold nanoparticles to develop a detection signal. By employing both THF stereochemistries in different thyclotide sequences, a prototype microfluidic assay was developed to detect synthetic HIV-1 RNA and signal its presence using silver-based enhancement of surface-bound gold nanoparticles. The limit of detection for this assay was 0.5 pM of synthetic HIV-1 RNA, which is a significant (∼100-fold) improvement over earlier PNA-based detection systems. Concentration-dependent variation in detection signal intensity allows for semi-quantitative determination of different RNA concentrations. A scrambled RNA control sequence does not interfere with detection. All results were obtained without using enzymatic amplification. The thyclotides in this study may be used in more advanced diagnostic technologies.

核酸检测常用来诊断病原性和遗传性疾病。基于聚合酶链反应（PCR）的诊断通常用于扩增和标记目标核酸的存在，但基于PCR的方法难以在患者的护理点和资源有限的环境中部署。本研究报告了一种新的核酸检测方法，利用化学修饰的PNAs直接检测靶RNA而不需要酶扩增。通过将反式3,4-二氨基四氢呋喃单元整合到PNA主链中，THF-PNAs（也称为thyclotides）设计具有增强的与靶核酸的结合亲和力和促进thyclotide-to-thyclotide结合而不是核酸结合的生物正交特性。具体来说，R，R- thf单体在thyclotide主链内的结合促进了有利于与靶核酸结合的右手螺旋。含有S，S- thf单体的生物正交thyclotide促进左旋螺旋，阻止其与天然核酸结合。互补的生物正交thyclotides可以在存在竞争RNA序列的情况下相互结合，并且这些独特的性质与金纳米颗粒结合使用以产生检测信号。通过在不同的thyclotide序列中使用这两种THF立体化学，开发了一种原型微流控分析来检测合成的HIV-1 RNA，并使用银基增强表面结合金纳米颗粒来表明其存在。该检测的检测限为0.5 pM的合成HIV-1 RNA，与早期基于pna的检测系统相比，这是一个显着（约100倍）的改进。检测信号强度的浓度依赖性变化允许半定量测定不同的RNA浓度。打乱的RNA控制序列不会干扰检测。所有结果均未使用酶扩增。本研究中的thyclotides可用于更先进的诊断技术。

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引用次数: 0

A silent speech interface with machine learning recognition model using microneedle array electrodes and polymer-based strain sensors 基于微针阵列电极和聚合物应变传感器的无声语音接口与机器学习识别模型

IF 7.6 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports

Pub Date : 2025-11-21 DOI: 10.1016/j.snr.2025.100407

Sheng-Kai Lin , Jui-Hua Lee , Hao-Sin Tsai , Yen-Chun Chen , Ming-Xiang Zhang , Wen-Cheng Kuo , Yao-Joe Yang

Silent speech interfaces (SSIs) recognize verbal expressions when speech signals are not accessible and serve as promising translator tools for people with voice disorder conditions. This work presents a wearable electromyogram (EMG)-based SSI device utilizing five microneedle array (MNA) electrodes and a conductive polymer-based strain sensor. An AI speech recognition model, which processes the EMG and strain signals, was implemented to enable assisted speaking without relying on the vocal folds. The proposed MNA electrodes can bypass the electric barrier of the stratum corneum layer of human skin and significantly enhance signal quality without the need for skin abrasion or conductive gel during electrode application. To enhance recognition accuracy, a conductive polymer-based strain sensor is used to measure the strain variation induced by the movement of the mandible bone during silent speech. The AI speech recognition model exhibited a solid word error rate (WER) (8.5%) for a dataset of 1,396 words. High recognition accuracy (>90%) was achieved on various datasets covering commonly used words and easily confusable word pairs. This proposed wearable SSI potentially helps people with vocal cord injuries regain their ability to speak, and potentially enables human interactions in special situations and environments.

无声语音接口（Silent speech interface, ssi）是一种在语音信号不可达的情况下识别语言表达的工具，为语音障碍患者提供了一种很有前途的翻译工具。这项工作提出了一种基于肌电图（EMG）的可穿戴式SSI设备，该设备利用五个微针阵列（MNA）电极和一个导电聚合物应变传感器。人工智能语音识别模型可以处理肌电图和应变信号，从而实现不依赖声带的辅助说话。所提出的MNA电极可以绕过人体皮肤角质层的电屏障，在电极应用过程中不需要皮肤磨损或导电凝胶，显著提高信号质量。为了提高识别精度，采用导电聚合物应变传感器测量无声说话时下颌骨运动引起的应变变化。人工智能语音识别模型在1396个单词的数据集上显示出稳定的单词错误率（WER）（8.5%）。在涵盖常用词和易混淆词对的各种数据集上，实现了较高的识别准确率（>90%）。这种可穿戴式SSI有可能帮助声带损伤患者恢复说话能力，并有可能在特殊情况和环境中实现人类互动。

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引用次数: 0

Unveiling MOF-derived CuFe2O4 as a catalytic promoter of Ru(bpy)32⁺ ECL: A CRISPR/Cas13a-based strategy for miR-17 Detection 揭示mof衍生CuFe2O4作为Ru(bpy)32 + ECL的催化促进剂：一种基于CRISPR/ cas13的miR-17检测策略

IF 7.6 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports

Pub Date : 2025-11-19 DOI: 10.1016/j.snr.2025.100406

Yanli Sun , Hongying Li , Tao Wang , Kai Zhang , Xueliang Wang

MicroRNA-17 (miR-17) is a critical biomarker linked to various cancers and diseases, underscoring the need for sensitive and selective detection methods. Herein, an ultra-sensitive electrochemiluminescent (ECL) sensor for miR-17 detection utilizing MOF-derived porous CuFe₂O₄ nanospheres and CRISPR/Cas13a trans-cleavage-triggered hybridization chain reaction (HCR) amplification was presented. The porous CuFe₂O₄ nanospheres possessed a high surface area and excellent catalytic activity, significantly enhancing ECL emission. To the best of our knowledge, this is the first report demonstrating that MOF-derived CuFe₂O₄ can effectively enhance the electrochemiluminescence of Ru(bpy)₃²⁺ for miR-17 detection. The CRISPR/Cas13a system provided miR-17 recognition and trans-cleavage activity, triggering HCR for signal amplification. Under optimized conditions, the biosensor exhibited a wide linear detection range from 20 aM to 5000 aM, with a detection limit of 9.23 aM. The system discriminated miR-17 from closely related miRNAs with negligible cross-reactivity, which demonstrated its outstanding selectivity. Practical applicability was confirmed by successful quantification of miR-17 in diluted human serum which achieved recovery rates between 95.2% and 104.8%. The results validated the biosensor’s potential for early disease diagnosis and clinical monitoring of miR-17-associated disorders.

MicroRNA-17 （miR-17）是与各种癌症和疾病相关的关键生物标志物，强调了对敏感和选择性检测方法的需求。本文提出了一种用于检测miR-17的超灵敏电化学发光（ECL）传感器，该传感器利用mof衍生的多孔CuFe2O4纳米球和CRISPR/Cas13a反式切割触发的杂交链反应（HCR）扩增。多孔CuFe2O4纳米球具有高的比表面积和优异的催化活性，显著增强了ECL发射。据我们所知，这是第一篇证明mof衍生的CuFe2O4可以有效增强Ru(bpy)32⁺用于miR-17检测的电化学发光的报道。CRISPR/Cas13a系统提供miR-17识别和反式切割活性，触发HCR进行信号放大。在优化条件下，该传感器在20 ~ 5000 aM范围内具有较宽的线性检测范围，检出限为9.23 aM。该系统以可忽略的交叉反应性将miR-17与密切相关的mirna区分开来，这表明其具有出色的选择性。miR-17在稀释后的人血清中成功定量，回收率在95.2%至104.8%之间，证实了其实用性。结果验证了该生物传感器在mir -17相关疾病的早期疾病诊断和临床监测方面的潜力。

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引用次数: 0

Electrochemical sensors: a review of group 10 and 11 metal composites for the detection of β-Lactam in aqueous media 电化学传感器：用于检测水介质中β-内酰胺的基团10和11金属复合材料的综述

IF 7.6 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports

Pub Date : 2025-11-13 DOI: 10.1016/j.snr.2025.100403

Opeyemi A. Iresemowo, Vincent O. Nyamori, Olatunde S. Olatunji

In recent years, the continuous release of antibiotics into the ecosystem has revealed significant downstream effects on a widespread population, leading to fatality and various deleterious impacts on the health of biological systems. Consequently, the development and accessibility of effective and straightforward recognition techniques for these pollutants have become critically imperative. Electrochemical strategies, characterised by their compactness, cost-effectiveness, and sensitivity, present a viable method for investigating antibiotics, offering information even in remote locations. Despite the availability of some commercially produced environmental sensors, they exhibit limitations such as cross-sensitivity, susceptibility to environmental interference, and drift over time. In contrast, electrochemical sensing offers distinct advantages, including rapid results generation, low detection limit, and portability. This review paper comprehensively addresses various aspects, encompassing sensor types, sensing materials, sensing techniques, and challenges associated with antibiotic sensing. The focus is mainly on β-lactam antibiotics due to their widespread use, prevalence, distribution, and adverse environmental and human health impacts. The review examines recent trends in utilising metallic nanoparticles, such as gold, silver, copper, and palladium, in conjunction with carbon-based materials for the design of electrochemical sensors that detect β-lactam antibiotics. Detailed discussions elucidate diverse mechanisms underlying the electrochemical sensing of β-lactam antibiotics while concurrently addressing existing challenges and providing future recommendations.

近年来，抗生素不断释放到生态系统中，对广泛的人群产生了显著的下游影响，导致死亡和对生物系统健康的各种有害影响。因此，开发和获取这些污染物的有效和直接的识别技术已变得至关重要。电化学策略以其紧凑、成本效益和灵敏度为特征，为研究抗生素提供了一种可行的方法，即使在偏远地区也能提供信息。尽管有一些商业生产的环境传感器，但它们表现出交叉灵敏度、对环境干扰的易感性和随时间漂移等局限性。相比之下，电化学传感具有明显的优势，包括快速生成结果，低检测限和便携性。这篇综述文章全面解决了各个方面，包括传感器类型，传感材料，传感技术，以及与抗生素传感相关的挑战。由于β-内酰胺类抗生素的广泛使用、流行、分布以及对环境和人类健康的不利影响，重点主要放在β-内酰胺类抗生素上。这篇综述探讨了利用金属纳米颗粒（如金、银、铜和钯）与碳基材料结合设计检测β-内酰胺类抗生素的电化学传感器的最新趋势。详细的讨论阐明了β-内酰胺类抗生素电化学传感的多种机制，同时解决了现有的挑战并提出了未来的建议。

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