Lung surfactant inhibition and cytotoxicity at the air-liquid interface of dry particle aerosols

Abstract

Industrial processes generate chemicals that have the potential to be aerosolized and inhaled by workers, thereby posing health risks. Traditional toxicology methods employing animal models cannot keep up with the pace of emerging hazards. Nascent in vitro practices face challenges regarding translatability to the real world. To address this critical gap, this study demonstrated a workflow utilizing aerosol characterization in a more realistic exposure scenario: dry powder aerosolization onto the air-liquid interface of lung cells. This study delves into biophysical aspects of lung function by examining lung surfactant inhibition. A set of particulates, including aluminum, aluminum oxide, carbon nanotubes, diesel particulate matter, and colloidal silica, was selected for investigation. Particles were in the respirable regime, with mean aerodynamic diameters ranging from 111 to 162 nm by number and 369–2884 nm by mass. Carbon nanotubes and colloidal silica were identified as surfactant inhibitors. Aerosol doses reduced cell viability, up to 38%, with the most pronounced effects observed in response to exposure to aluminum and diesel particulate matter. Dry particle exposure at the air-liquid interface shows promise even at low doses, compared with nebulization or inoculation to submerged cultures. Our findings underscore the potential of this innovative approach for assessing the hazards of aerosolized particulates and emerging contaminants, offering a more accurate representation of real-world exposure scenarios.