Valorisation of Spent Yeast Fermentation Media through Compositional-Analysis-Directed Supplementation

Laura Murphy, Ciara D. Lynch, David J O’Connell
{"title":"Valorisation of Spent Yeast Fermentation Media through Compositional-Analysis-Directed Supplementation","authors":"Laura Murphy, Ciara D. Lynch, David J O’Connell","doi":"10.3390/applmicrobiol4020065","DOIUrl":null,"url":null,"abstract":"Spent fermentation media from bioprocessing represent a significant waste stream, and interest in recycling them as part of the developing circular bioeconomy is growing. The potential to reuse yeast spent culture media (YSM) to feed secondary bacterial fermentations producing recombinant protein was investigated in this study. Elemental and amino acid compositional analysis using inductively coupled plasma mass spectrometry (ICP-MS) and LC-MS/MS identified significant differences in the concentrations of 6 elements and 18/20 amino acids in YSM compared with rich microbiological media (LB). Restoration of levels of magnesium and sodium through addition of their salts and amino acids from tryptone supplementation led to the expression of equivalent titres of recombinant proteins by E. coli (0.275 g/L), compared to that in LB media (0.296 g/L) and BMMY media (0.294 g/L) in shake flask culture. When this supplementation strategy was employed in a bioreactor system, we observed a significant increase in recombinant protein titre using the supplemented YSM (2.29 (±0.02) g/L) over that produced using LB media (1.29 (±0.09) g/L). This study demonstrates through highly sensitive compositional analysis and identification of supplementation strategies the potential to valorise spent media from yeast fermentations that underpin industrial processes of significant scale, creating a circular approach to waste stream management.","PeriodicalId":8080,"journal":{"name":"Applied microbiology","volume":"17 26","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3390/applmicrobiol4020065","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Spent fermentation media from bioprocessing represent a significant waste stream, and interest in recycling them as part of the developing circular bioeconomy is growing. The potential to reuse yeast spent culture media (YSM) to feed secondary bacterial fermentations producing recombinant protein was investigated in this study. Elemental and amino acid compositional analysis using inductively coupled plasma mass spectrometry (ICP-MS) and LC-MS/MS identified significant differences in the concentrations of 6 elements and 18/20 amino acids in YSM compared with rich microbiological media (LB). Restoration of levels of magnesium and sodium through addition of their salts and amino acids from tryptone supplementation led to the expression of equivalent titres of recombinant proteins by E. coli (0.275 g/L), compared to that in LB media (0.296 g/L) and BMMY media (0.294 g/L) in shake flask culture. When this supplementation strategy was employed in a bioreactor system, we observed a significant increase in recombinant protein titre using the supplemented YSM (2.29 (±0.02) g/L) over that produced using LB media (1.29 (±0.09) g/L). This study demonstrates through highly sensitive compositional analysis and identification of supplementation strategies the potential to valorise spent media from yeast fermentations that underpin industrial processes of significant scale, creating a circular approach to waste stream management.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
通过成分分析定向补充废酵母发酵培养基的价值
生物加工过程中产生的废发酵培养基是一种重要的废物流,作为发展中的循环生物经济的一部分,人们对回收利用这些培养基的兴趣与日俱增。本研究调查了重复使用酵母废培养基(YSM)来为生产重组蛋白质的二次细菌发酵提供养料的潜力。使用电感耦合等离子体质谱法(ICP-MS)和液相色谱-质谱法(LC-MS/MS)进行元素和氨基酸组成分析,发现与富含微生物培养基(LB)相比,YSM 中 6 种元素和 18/20 种氨基酸的浓度存在显著差异。在摇瓶培养中,通过添加镁盐和钠盐以及补充胰蛋白胨中的氨基酸来恢复镁和钠的含量,使大肠杆菌表达的重组蛋白滴度(0.275 克/升)与在 LB 培养基(0.296 克/升)和 BMMY 培养基(0.294 克/升)中表达的滴度相当。在生物反应器系统中采用这种补充策略时,我们观察到使用补充 YSM 的重组蛋白滴度(2.29 (±0.02) g/L)比使用 LB 培养基的重组蛋白滴度(1.29 (±0.09) g/L)显著增加。这项研究通过高灵敏度的成分分析和补充策略的确定,证明了酵母发酵产生的废培养基的增值潜力,这些培养基是大规模工业流程的基础,创造了一种废物流管理的循环方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Production of Functional Vinegar Enriched with γ-Aminobutyric Acid through Serial Co-Fermentation of Lactic Acid and Acetic Acid Bacteria Using Rice Wine Lees Genomic Characterization of Selected Escherichia coli Strains from Catfish (Clarias gariepinus) in Nigeria Metatranscriptomic Analysis of Argentinian Kefirs Varying in Apparent Viscosity Multiplex-PCR Detection of an Atypical Leuconostoc mesenteroides subsp. jonggajibkimchii Phenotype Dominating the Terminal Spoilage Microbial Association of a Fresh Greek Whey Cheese Stored at 4 °C in Vacuum Interaction between Trichoderma sp., Pseudomonas putida, and Two Organic Amendments on the Yield and Quality of Strawberries (Fragaria x annanasa cv. San Andreas) in the Huaral Region, Peru
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1