Quantification of Glucose and Fructose in Georgian Semi-sweet Wines

Abstract

One of the important parameters for wine certification is the quantitative determination of glucose and fructose, for which the highly efficient liquid chromatographic method is tested and is accurate. The quantitative determination of glucose and fructose in semi-sweet wines using high performance liquid chromatography is discussed in the article. A high-performance liquid chromatograph (HPLC) with a refractive index detector (RID) and a reversed-phase alkali amine chromatography column was used during the analysis. The method showed a high correlation coefficient during calibration (R2 = 0.99). With the given method, the concentration of glucose and fructose in semi-sweet wines of some denominations of Georgian production was measured. Quantification of these sugars is of particular importance in assessing the sweetness profile of wine and ensuring compliance with quality standards. For analysis, semi-sweet wines of Georgian production were used, in which the total amount of glucose and fructose is within the norm according to the Georgian Government Resolution #524.