Pendekatan One Health: Deteksi Gen blaZ dari Isolat Staphylococcus aureus Asal Peternakan Sapi Perah di Sulawesi Selatan

Abstract

Antibiotic resistance is an important health problem worldwide that affects human and animal health. The presence of resistance genes in Staphylococcus aureus Isolates may contribute to the development of antibiotic resistance. The study was aimed to identify the presence of the blaZ gene from S. aureus Isolates that were resistant to penicillin G from dairy farming areas in South Sulawesi Province. The sample was obtained from 20 locations of dairy farms spread across Enrekang Regency. Identification of S. aureus was carried out by phenotypic and genotypic methods. Isolates of S. aureus that were resistant to penicillin G came from humans (n=6), dairy cattle (n=10), and dangke products (n=2) were obtained from the results of sensitivity tests to penicillin G (10 U) antibiotics with Kirby-Bauer disc diffusion method. Detection of blaZ gene using conventional polymerase chain reaction (PCR) method. The results showed that Isolates from humans, dairy cattle, and dangke products detected the presence of the blaZ gene by 100% respectively. The blaZ gene found n human Isolates, animals, and dangke products has the potential to spread between Isolates and has the potential to cause human health problems.