Pub Date : 2024-06-10DOI: 10.19087/jveteriner.2024.25.1.122
Sartika Juwita, Agustina Indrawati, Retno Damajanti, Safika, N. Mayasari, E. Purwanto, Handayani Halik, M. Ramadhan, Suhartila
Antibiotic resistance is an important health problem worldwide that affects human and animal health. The presence of resistance genes in Staphylococcus aureus Isolates may contribute to the development of antibiotic resistance. The study was aimed to identify the presence of the blaZ gene from S. aureus Isolates that were resistant to penicillin G from dairy farming areas in South Sulawesi Province. The sample was obtained from 20 locations of dairy farms spread across Enrekang Regency. Identification of S. aureus was carried out by phenotypic and genotypic methods. Isolates of S. aureus that were resistant to penicillin G came from humans (n=6), dairy cattle (n=10), and dangke products (n=2) were obtained from the results of sensitivity tests to penicillin G (10 U) antibiotics with Kirby-Bauer disc diffusion method. Detection of blaZ gene using conventional polymerase chain reaction (PCR) method. The results showed that Isolates from humans, dairy cattle, and dangke products detected the presence of the blaZ gene by 100% respectively. The blaZ gene found n human Isolates, animals, and dangke products has the potential to spread between Isolates and has the potential to cause human health problems.
{"title":"Pendekatan One Health: Deteksi Gen blaZ dari Isolat Staphylococcus aureus Asal Peternakan Sapi Perah di Sulawesi Selatan","authors":"Sartika Juwita, Agustina Indrawati, Retno Damajanti, Safika, N. Mayasari, E. Purwanto, Handayani Halik, M. Ramadhan, Suhartila","doi":"10.19087/jveteriner.2024.25.1.122","DOIUrl":"https://doi.org/10.19087/jveteriner.2024.25.1.122","url":null,"abstract":"Antibiotic resistance is an important health problem worldwide that affects human and animal health. The presence of resistance genes in Staphylococcus aureus Isolates may contribute to the development of antibiotic resistance. The study was aimed to identify the presence of the blaZ gene from S. aureus Isolates that were resistant to penicillin G from dairy farming areas in South Sulawesi Province. The sample was obtained from 20 locations of dairy farms spread across Enrekang Regency. Identification of S. aureus was carried out by phenotypic and genotypic methods. Isolates of S. aureus that were resistant to penicillin G came from humans (n=6), dairy cattle (n=10), and dangke products (n=2) were obtained from the results of sensitivity tests to penicillin G (10 U) antibiotics with Kirby-Bauer disc diffusion method. Detection of blaZ gene using conventional polymerase chain reaction (PCR) method. The results showed that Isolates from humans, dairy cattle, and dangke products detected the presence of the blaZ gene by 100% respectively. The blaZ gene found n human Isolates, animals, and dangke products has the potential to spread between Isolates and has the potential to cause human health problems.","PeriodicalId":17749,"journal":{"name":"Jurnal Veteriner","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141366337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-10DOI: 10.19087/jveteriner.2024.25.1.143
Forcep Rio Indaryanto, Mohammad Mukhlis Kamal, N. A. Butet, Ridwan Affandi, Risa Tiuria
Anisakis is a zoonotic nematode that causes anisakiasis in Europe and Asia.Food safety has recently become a major concern for consumers; therefore, accurate identification of Anisakis larvae is necessary for the correct diagnosis of Anisakis infection in humans and fish and for improving food safety. From 2001 to 2023, Anisakis examinations were carried out in 33 areas of Indonesian territory. Morphometric Anisakis of Indonesia generally have a body length of 0.10-34.50 mm with a diameter of 0.30-0.42 mm. Based on morphological and genetic identification, identified six species anisakis in Indonesia (A. simplex, A. physeteris, A. pegreffii, A. berlandi, A. typica and Anisakis sp. HC-2005), or identified six Anisakis genotypes based on genetic identification, namely A. typica (s.s), A. typica var Indonesiansis, A. physeteris, A. pegreffii, A. berlandi andAnisakis sp. HC-2005. Decapterus sp., Thyrsitoidesmarleyi, Euthynnusafifinis, Auxis rochei, Gempylus serpens, Katsuwonus pelamis and Trichiurus lepturus have a high prevalence (>90%), indicating that they are often infected with Anisakis. Anisakis spread throughout the waters in Indonesia with various types of fish as hosts and A. typica is the predominant species in Indonesia.
{"title":"Article Review: Identifications and Geographic Distribution of Six Anisakis Species (Nematoda: Anisakidae) in Indonesia","authors":"Forcep Rio Indaryanto, Mohammad Mukhlis Kamal, N. A. Butet, Ridwan Affandi, Risa Tiuria","doi":"10.19087/jveteriner.2024.25.1.143","DOIUrl":"https://doi.org/10.19087/jveteriner.2024.25.1.143","url":null,"abstract":"Anisakis is a zoonotic nematode that causes anisakiasis in Europe and Asia.Food safety has recently become a major concern for consumers; therefore, accurate identification of Anisakis larvae is necessary for the correct diagnosis of Anisakis infection in humans and fish and for improving food safety. From 2001 to 2023, Anisakis examinations were carried out in 33 areas of Indonesian territory. Morphometric Anisakis of Indonesia generally have a body length of 0.10-34.50 mm with a diameter of 0.30-0.42 mm. Based on morphological and genetic identification, identified six species anisakis in Indonesia (A. simplex, A. physeteris, A. pegreffii, A. berlandi, A. typica and Anisakis sp. HC-2005), or identified six Anisakis genotypes based on genetic identification, namely A. typica (s.s), A. typica var Indonesiansis, A. physeteris, A. pegreffii, A. berlandi andAnisakis sp. HC-2005. Decapterus sp., Thyrsitoidesmarleyi, Euthynnusafifinis, Auxis rochei, Gempylus serpens, Katsuwonus pelamis and Trichiurus lepturus have a high prevalence (>90%), indicating that they are often infected with Anisakis. Anisakis spread throughout the waters in Indonesia with various types of fish as hosts and A. typica is the predominant species in Indonesia.","PeriodicalId":17749,"journal":{"name":"Jurnal Veteriner","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141361223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-10DOI: 10.19087/jveteriner.2024.25.1.13
Diana Meliani Sabat, F. T. Haryadi, Nono Ngadiyono
The most popular business for the people of Kupang City is the pig farming business. In doing their business, farmers certainly have certain motivations. So far, motivation is often seen as one complete variable, even though in doing business farmers do not only look at one reason, but see multidimensionally from various dimensions. The dimensions in question are economic, socio-cultural and household food waste utilization motives. This study was aimed to evaluate and analyze the multidimensionality of priority motivations in pig farming and determine which motives are most prioritized by farmers in conducting a pig business in Kupang City. There were 20 pig farmers involved in this study selected using purposive sampling method located in three sub-districts in Kupang City. The variables measured were farmers’ motivations (economic motive, socio-cultural motive, and food waste utilization motive, farmer time and labor). Data analysis was conducted using the Analytic Hierarchy Process (AHP). The results showed that socio-cultural motives had the highest priority value (0.4032), followed by economic motives (0.3162) and household food utilization motives (0.2806).
{"title":"Multidimensional Analyses of Motivations for Pig Farming by the Residents of Kupang, Nusa Tenggara Timur, Indonesia","authors":"Diana Meliani Sabat, F. T. Haryadi, Nono Ngadiyono","doi":"10.19087/jveteriner.2024.25.1.13","DOIUrl":"https://doi.org/10.19087/jveteriner.2024.25.1.13","url":null,"abstract":"The most popular business for the people of Kupang City is the pig farming business. In doing their business, farmers certainly have certain motivations. So far, motivation is often seen as one complete variable, even though in doing business farmers do not only look at one reason, but see multidimensionally from various dimensions. The dimensions in question are economic, socio-cultural and household food waste utilization motives. This study was aimed to evaluate and analyze the multidimensionality of priority motivations in pig farming and determine which motives are most prioritized by farmers in conducting a pig business in Kupang City. There were 20 pig farmers involved in this study selected using purposive sampling method located in three sub-districts in Kupang City. The variables measured were farmers’ motivations (economic motive, socio-cultural motive, and food waste utilization motive, farmer time and labor). Data analysis was conducted using the Analytic Hierarchy Process (AHP). The results showed that socio-cultural motives had the highest priority value (0.4032), followed by economic motives (0.3162) and household food utilization motives (0.2806).","PeriodicalId":17749,"journal":{"name":"Jurnal Veteriner","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141361776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-10DOI: 10.19087/jveteriner.2024.25.1.21
M. Djaelani, Lestari Milatul Khusnia, Sunarno
Cultivation of red tilapia (Oreochromis niloticus) requires good water quality. Physiological processes and organ structures can be disrupted when water quality including dissolved oxygen, water temperature, pH, salinity and ammonia exceeds the normal threshold. Aeration and filtration in fish farming can help improve water quality which affects the improvement of physiological processes and spleen function of red tilapia. The study was purposed to analyze the use of aerators, filters and a combination of both on the histomorphometry and structure of the spleen of red tilapia. This study used a completely randomized design with a 2×2 factorial arrangement. Each main factor consists of two levels, such as aeration i.e., single aeration and double aeration and filtration i.e., without filter and with filter. The experimental fish in the study were 24 red tilapia fish which were divided into four treatment groups, which included single aeration without filter, double aeration without filter, single aeration with filter, and double aeration with filter. The results showed that the interaction between aeration and filters had no significant effect (P>0.05) on spleen weight, Relative Spleen Weight (RSW), red pulp diameter, white pulp diameter, melanomacrophage center (MMC) diameter, and MMC cell diameter. The conclusion of this study was that the aeration, filters and combination of aeration and filters could maintain the chemical quality of water so there was no adverse effect on the histomorphometry and structure of the spleen of red tilapia
{"title":"Histomorfometri Limpa Ikan Nila Merah (Oreochromis niloticus) yang Dibudidayakan dengan Aerasi dan Filtrasi Berbeda","authors":"M. Djaelani, Lestari Milatul Khusnia, Sunarno","doi":"10.19087/jveteriner.2024.25.1.21","DOIUrl":"https://doi.org/10.19087/jveteriner.2024.25.1.21","url":null,"abstract":"Cultivation of red tilapia (Oreochromis niloticus) requires good water quality. Physiological processes and organ structures can be disrupted when water quality including dissolved oxygen, water temperature, pH, salinity and ammonia exceeds the normal threshold. Aeration and filtration in fish farming can help improve water quality which affects the improvement of physiological processes and spleen function of red tilapia. The study was purposed to analyze the use of aerators, filters and a combination of both on the histomorphometry and structure of the spleen of red tilapia. This study used a completely randomized design with a 2×2 factorial arrangement. Each main factor consists of two levels, such as aeration i.e., single aeration and double aeration and filtration i.e., without filter and with filter. The experimental fish in the study were 24 red tilapia fish which were divided into four treatment groups, which included single aeration without filter, double aeration without filter, single aeration with filter, and double aeration with filter. The results showed that the interaction between aeration and filters had no significant effect (P>0.05) on spleen weight, Relative Spleen Weight (RSW), red pulp diameter, white pulp diameter, melanomacrophage center (MMC) diameter, and MMC cell diameter. The conclusion of this study was that the aeration, filters and combination of aeration and filters could maintain the chemical quality of water so there was no adverse effect on the histomorphometry and structure of the spleen of red tilapia","PeriodicalId":17749,"journal":{"name":"Jurnal Veteriner","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141361906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-10DOI: 10.19087/jveteriner.2024.25.1.81
Rafi Ahmad Farhan, I. N. Suartha, L. M. Sudimartini
Atopic dermatitis is one of the most common diseases in dogs that is multifactorial and usually attacks dogs aged between 6-36 months. This study was aimed to determine the histopathological appearance of the skin of dogs suffering from atopic dermatitis after treated with eco enzyme. Eco enzyme is a liquid product resulting from the fermentation of organic kitchen waste product such as fruit and vegetable dregs, molasses, and water. This study used five dogs suffering from atopic dermatitis which were divided into two groups. Group A: three dogs samples were bathed with 10% eco enzyme liquid every three days for 28 days. In Group B: two samples of dogs were bathed with 10% eco enzyme every three days until the 9th day, followed by a 2% eco enzyme bath once a week (13th, 20th, 27th day). Both treatment groups started from day 0 until day 28. Skin samples were taken using the biopsy method and histopathological changes were observed by making histopathological skin preparations stained with Hematoxylin and Eosin. Observations of the preparations were carried out using a light microscope. Data were analyzed using the Analysis of Variance test followed by the Duncan multiple range test and the Kruskall Wallis test followed by the Mann-Whitney test, then the data was explained descriptively. The results of the Anova test showed that the thickness of the dogs epidermis treated with 2% and 10% eco enzyme was significantly different (P<0.05). The Kruskall Wallis test for scoring inflammatory cell infiltration and degeneration showed that there was a significant difference (P<0.05) in the administration of 2% and 10% eco enzyme. Eco enzyme liquid is able to reduce atopic dermatitis lesions in dogs. Giving eco enzyme at a concentration of 10% and followed by a concentration of 2% with an extended application time can return the histological structure of the dog’s skin to normal, especially the thickness of the epidermis, reduce inflammatory cell infiltration, and reduce degeneration.
{"title":"Gambaran Histopatologi Kulit Anjing Penderita Dermatitis Atopik Pascapemberian Eco Enzyme","authors":"Rafi Ahmad Farhan, I. N. Suartha, L. M. Sudimartini","doi":"10.19087/jveteriner.2024.25.1.81","DOIUrl":"https://doi.org/10.19087/jveteriner.2024.25.1.81","url":null,"abstract":"Atopic dermatitis is one of the most common diseases in dogs that is multifactorial and usually attacks dogs aged between 6-36 months. This study was aimed to determine the histopathological appearance of the skin of dogs suffering from atopic dermatitis after treated with eco enzyme. Eco enzyme is a liquid product resulting from the fermentation of organic kitchen waste product such as fruit and vegetable dregs, molasses, and water. This study used five dogs suffering from atopic dermatitis which were divided into two groups. Group A: three dogs samples were bathed with 10% eco enzyme liquid every three days for 28 days. In Group B: two samples of dogs were bathed with 10% eco enzyme every three days until the 9th day, followed by a 2% eco enzyme bath once a week (13th, 20th, 27th day). Both treatment groups started from day 0 until day 28. Skin samples were taken using the biopsy method and histopathological changes were observed by making histopathological skin preparations stained with Hematoxylin and Eosin. Observations of the preparations were carried out using a light microscope. Data were analyzed using the Analysis of Variance test followed by the Duncan multiple range test and the Kruskall Wallis test followed by the Mann-Whitney test, then the data was explained descriptively. The results of the Anova test showed that the thickness of the dogs epidermis treated with 2% and 10% eco enzyme was significantly different (P<0.05). The Kruskall Wallis test for scoring inflammatory cell infiltration and degeneration showed that there was a significant difference (P<0.05) in the administration of 2% and 10% eco enzyme. Eco enzyme liquid is able to reduce atopic dermatitis lesions in dogs. Giving eco enzyme at a concentration of 10% and followed by a concentration of 2% with an extended application time can return the histological structure of the dog’s skin to normal, especially the thickness of the epidermis, reduce inflammatory cell infiltration, and reduce degeneration.","PeriodicalId":17749,"journal":{"name":"Jurnal Veteriner","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141363205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-10DOI: 10.19087/jveteriner.2024.25.1.73
Mawar Datu Allo Dendang, Wayan Bebas, I. G. B. Trilaksana
One of the unique ornamental chickens that is in great demand by ornamental chicken enthusiasts is the long-tailed chicken or onagadori chicken originating from the city of Nankoku Kochi, Japan. This study was aimed to determine the effect of the frequency of semen collection on spermatozoa quality in onagadori chickens. In the study used a completely randomized design (CRD) with three treatment groups using eight onagadori chickens with an age range of 6-7 months. The first treatment (P1), was cement collection carried out every six days or once a week; the second treatment (P2) was cement collection carried out every three days or twice a week; the third treatment (P3) was cement collection carried out every two days or thrice a week. Each treatment was repeated three times. Observations were made on the quality of onagadori chicken spermatozoa. Semen collection once a week produces a volume of 0.087 mL; concentration 4.7 billion cells/mL; motility 84.66%; abnormality 8.46%; viability 81.44%. Semen collection twice a week produces a volume of 0.09 mL; concentration 4.9 billion cells/mL; motility 83.22%; abnormality 9.1%; viability 83.87. Semen collection three times a week produces a volume of 0.09 mL; concentration 4.7 billion cells/mL; motility 83.57%; abnormality 8.54%; viability 82.32%. The data obtained was analyzed by Analysis of Variant and then followed by the Least Significant Difference (LSD) test. The results showed that the frequency of semen collection at P1, P2, and P3 on volume and concentration, motility, and abnormalities yielded no significant differences (P>0.05), while viability showed significant differences (P<0.05).
{"title":"Penampungan Semen yang Terlalu Sering Menurunkan Daya HidupSpermazoa pada Ayam Onagadori","authors":"Mawar Datu Allo Dendang, Wayan Bebas, I. G. B. Trilaksana","doi":"10.19087/jveteriner.2024.25.1.73","DOIUrl":"https://doi.org/10.19087/jveteriner.2024.25.1.73","url":null,"abstract":"One of the unique ornamental chickens that is in great demand by ornamental chicken enthusiasts is the long-tailed chicken or onagadori chicken originating from the city of Nankoku Kochi, Japan. This study was aimed to determine the effect of the frequency of semen collection on spermatozoa quality in onagadori chickens. In the study used a completely randomized design (CRD) with three treatment groups using eight onagadori chickens with an age range of 6-7 months. The first treatment (P1), was cement collection carried out every six days or once a week; the second treatment (P2) was cement collection carried out every three days or twice a week; the third treatment (P3) was cement collection carried out every two days or thrice a week. Each treatment was repeated three times. Observations were made on the quality of onagadori chicken spermatozoa. Semen collection once a week produces a volume of 0.087 mL; concentration 4.7 billion cells/mL; motility 84.66%; abnormality 8.46%; viability 81.44%. Semen collection twice a week produces a volume of 0.09 mL; concentration 4.9 billion cells/mL; motility 83.22%; abnormality 9.1%; viability 83.87. Semen collection three times a week produces a volume of 0.09 mL; concentration 4.7 billion cells/mL; motility 83.57%; abnormality 8.54%; viability 82.32%. The data obtained was analyzed by Analysis of Variant and then followed by the Least Significant Difference (LSD) test. The results showed that the frequency of semen collection at P1, P2, and P3 on volume and concentration, motility, and abnormalities yielded no significant differences (P>0.05), while viability showed significant differences (P<0.05).","PeriodicalId":17749,"journal":{"name":"Jurnal Veteriner","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141362703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-10DOI: 10.19087/jveteriner.2024.25.1.103
Dewa Ayu Agus Sri Laksemi, Luh Putu Ratna Sundari, Ni Luh Rustini, P. A. Damayanti, I. M. Sudarmaja, N. Linawati
Malaria drug resistance, both new and old drugs, causes severe symptoms and treatment failure. In order to develop new drug, we screen several plants that potentially used as antimalaria. This study aimed to screen and compare antimalaria activity of Citrus maxima, Anredera cordifolia, Solanum lycopersicum, combination Solanum lycopersicum-Malus domestica-Daucus carota in a mouse model. Balb/ c mice male were divided into six groups (n=5). The dried plants were extracted by maceration using 80% methanol. A 4-day suppressive test was done to ascertain the anti-plasmodial effect of those various plants. After inoculation with Plasmodium berghei, the treatment groups were given 1000 mg/kg of the extract, while 5% RPMI and DHP (Dihydroartemisinine Piperaquine) served as controls. The results of this study revealed that all extracts showed significantly lower parasitemia compared to the negative control. This study showed a percentage parasite suppression of 58.7, 84.3, 77.4, 61.4 and 83.9 % for Anredera cordifolia, Citrus maxima, Solanum lycopersicum, combination Solanum lycopersicum-Malus domestica-Daucus carota and Dihydroartemisinine Piperaquine, respectively. The highest parasite suppression (84.3%) was found in the group given Citrus maxima extract. This study concluded that the fruit extract of Citrus Maxima, Anredera Cordifolia, Solanum Lycopersicum-Malus Domestica-Daucus Carota have different antimalaria activity, Citrus maxima has the best potential as an antimalarial.
{"title":"Perbedaan Aktivitas Antimalaria Citrus maxima, Anredera cordifolia, Kombinasi Solanum lycopersicum-Malus domestica-Daucus carota pada Mencit Diinfeksi Plasmodium berghei)","authors":"Dewa Ayu Agus Sri Laksemi, Luh Putu Ratna Sundari, Ni Luh Rustini, P. A. Damayanti, I. M. Sudarmaja, N. Linawati","doi":"10.19087/jveteriner.2024.25.1.103","DOIUrl":"https://doi.org/10.19087/jveteriner.2024.25.1.103","url":null,"abstract":"Malaria drug resistance, both new and old drugs, causes severe symptoms and treatment failure. In order to develop new drug, we screen several plants that potentially used as antimalaria. This study aimed to screen and compare antimalaria activity of Citrus maxima, Anredera cordifolia, Solanum lycopersicum, combination Solanum lycopersicum-Malus domestica-Daucus carota in a mouse model. Balb/ c mice male were divided into six groups (n=5). The dried plants were extracted by maceration using 80% methanol. A 4-day suppressive test was done to ascertain the anti-plasmodial effect of those various plants. After inoculation with Plasmodium berghei, the treatment groups were given 1000 mg/kg of the extract, while 5% RPMI and DHP (Dihydroartemisinine Piperaquine) served as controls. The results of this study revealed that all extracts showed significantly lower parasitemia compared to the negative control. This study showed a percentage parasite suppression of 58.7, 84.3, 77.4, 61.4 and 83.9 % for Anredera cordifolia, Citrus maxima, Solanum lycopersicum, combination Solanum lycopersicum-Malus domestica-Daucus carota and Dihydroartemisinine Piperaquine, respectively. The highest parasite suppression (84.3%) was found in the group given Citrus maxima extract. This study concluded that the fruit extract of Citrus Maxima, Anredera Cordifolia, Solanum Lycopersicum-Malus Domestica-Daucus Carota have different antimalaria activity, Citrus maxima has the best potential as an antimalarial.","PeriodicalId":17749,"journal":{"name":"Jurnal Veteriner","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141365569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-10DOI: 10.19087/jveteriner.2024.25.1.55
Bagas Pria Prasetyo, Soedarmanto Indrajulianto, Ida Tjahajati, Imron Rosyadi, Akbar Wicaksono
Feline Infectious Peritonitis (FIP) is a fatal disease occurring in cats worldwide caused by Feline Coronavirus. Antemortem diagnosis of FIP is difficult due to non-specific clinical and laboratory signs. Rapid and reliable diagnosis is essential for prognostic purposes. The study was aimed to evaluate the serum biochemical profile and ascites of cats with effusive FIP. This study used five cats suspected of effusive FIP as a samples. Diagnosis was based on clinical examination, Rivalta and rapid test of FIP antibodies to ascites samples. Blood serum and ascites fluid were collected for examination of total protein, albumin, globulin and albumin/globulin (A/G) ratio. Clinical examination results showed that 5/5 (100%) cats had ascites. Rivalta test on ascites sample was positive for exudate and antibody rapid test was positive for FIP. The biochemical test showed an average total serum protein and ascites fluid of 7.14 ± 1.36 g/dL and 6.12 ± 0.33 g/dL (P<0.05); hypoalbuminemia was found in serum samples with an average of 1.94 ± 0.33 g/dL and ascites fluid albumin of 1.69 ± 0.32 g/dL (P<0.05), serum globulin had an average of 5.06 ± 1.32 g/dL and ascites globulin of 4.80 ± 0.88 g/dL (P>0.05). All cats had a low mean A/G ratio of 0.37 ± 0.36 in serum and 0.34 ± 0.01 in ascites (P>0.05). Concluded that the biochemical profile of serum and ascites in effusive FIP included normal total protein, hypoalbuminemia, normal serum globulin and decreased A/G ratio. Moreover, significant statistical discrepancy in serum and ascites biochemical profiles only occurred in total protein and albumin, but not in globulin and A/G ratio.
{"title":"Profil Biokimia Serum dan Cairan Asites Kasus Feline Infectious Peritonitis tipe Efusif","authors":"Bagas Pria Prasetyo, Soedarmanto Indrajulianto, Ida Tjahajati, Imron Rosyadi, Akbar Wicaksono","doi":"10.19087/jveteriner.2024.25.1.55","DOIUrl":"https://doi.org/10.19087/jveteriner.2024.25.1.55","url":null,"abstract":"Feline Infectious Peritonitis (FIP) is a fatal disease occurring in cats worldwide caused by Feline Coronavirus. Antemortem diagnosis of FIP is difficult due to non-specific clinical and laboratory signs. Rapid and reliable diagnosis is essential for prognostic purposes. The study was aimed to evaluate the serum biochemical profile and ascites of cats with effusive FIP. This study used five cats suspected of effusive FIP as a samples. Diagnosis was based on clinical examination, Rivalta and rapid test of FIP antibodies to ascites samples. Blood serum and ascites fluid were collected for examination of total protein, albumin, globulin and albumin/globulin (A/G) ratio. Clinical examination results showed that 5/5 (100%) cats had ascites. Rivalta test on ascites sample was positive for exudate and antibody rapid test was positive for FIP. The biochemical test showed an average total serum protein and ascites fluid of 7.14 ± 1.36 g/dL and 6.12 ± 0.33 g/dL (P<0.05); hypoalbuminemia was found in serum samples with an average of 1.94 ± 0.33 g/dL and ascites fluid albumin of 1.69 ± 0.32 g/dL (P<0.05), serum globulin had an average of 5.06 ± 1.32 g/dL and ascites globulin of 4.80 ± 0.88 g/dL (P>0.05). All cats had a low mean A/G ratio of 0.37 ± 0.36 in serum and 0.34 ± 0.01 in ascites (P>0.05). Concluded that the biochemical profile of serum and ascites in effusive FIP included normal total protein, hypoalbuminemia, normal serum globulin and decreased A/G ratio. Moreover, significant statistical discrepancy in serum and ascites biochemical profiles only occurred in total protein and albumin, but not in globulin and A/G ratio.","PeriodicalId":17749,"journal":{"name":"Jurnal Veteriner","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141365598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-10DOI: 10.19087/jveteriner.2024.25.1.132
Sylvia Kamil, I. Mahardika, I. K. Suardana
A vaccine against African Swine Fever (ASF) is urgently needed to reduce losses for pig farmers throughout the world. The study was aimed to test the immune response of mice to an ASF-DNA vaccine with the A224L and A276R genes with polymer, lipofectamine, and cholesterol adjuvants. Each mouse was vaccinated intramuscularly with 25 μg of plasmid encapsulated with the above adjuvants. Mouse sera were collected at weeks 1, 2, 3 and 4. Antibodies against A224L and A276R were detected by ELISA test using synthetic peptides as antigens. Optical density (OD) values were analysed statistically using the Analysis of Variance test with the SPSS Version 25. The results showed that the OD values of the serum of mice given the ASF-A224L and A276R DNA vaccines with polymer adjuvants (0.149), lipofectamine (0.080), and cholesterol (0.058), as well as control mice (0.020) differed significantly (p= 0.000), while the time of serum collection had no significant effect (p=0.517). The conclusion is that the OD values of mice sera after DNA-ASF-A224L and A276R encapsulated with polymer, lipofectamine and cholesterol were statistically significant, while sampling time did not show any statistically difference. Further studies are needed to improve the adjuvant mixing protocol with larger quantities of plasmids.Keywords:mice; DNA vaccine; ASF; polymer; ELISA
{"title":"Respons Imun Mencit terhadap Vaksin DNA Virus Demam Babi Afrika A224L dan A276R dengan Enkapsulasi Lipofektamin, Kolesterol dan Polimer","authors":"Sylvia Kamil, I. Mahardika, I. K. Suardana","doi":"10.19087/jveteriner.2024.25.1.132","DOIUrl":"https://doi.org/10.19087/jveteriner.2024.25.1.132","url":null,"abstract":"A vaccine against African Swine Fever (ASF) is urgently needed to reduce losses for pig farmers throughout the world. The study was aimed to test the immune response of mice to an ASF-DNA vaccine with the A224L and A276R genes with polymer, lipofectamine, and cholesterol adjuvants. Each mouse was vaccinated intramuscularly with 25 μg of plasmid encapsulated with the above adjuvants. Mouse sera were collected at weeks 1, 2, 3 and 4. Antibodies against A224L and A276R were detected by ELISA test using synthetic peptides as antigens. Optical density (OD) values were analysed statistically using the Analysis of Variance test with the SPSS Version 25. The results showed that the OD values of the serum of mice given the ASF-A224L and A276R DNA vaccines with polymer adjuvants (0.149), lipofectamine (0.080), and cholesterol (0.058), as well as control mice (0.020) differed significantly (p= 0.000), while the time of serum collection had no significant effect (p=0.517). The conclusion is that the OD values of mice sera after DNA-ASF-A224L and A276R encapsulated with polymer, lipofectamine and cholesterol were statistically significant, while sampling time did not show any statistically difference. Further studies are needed to improve the adjuvant mixing protocol with larger quantities of plasmids.Keywords:mice; DNA vaccine; ASF; polymer; ELISA","PeriodicalId":17749,"journal":{"name":"Jurnal Veteriner","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141362282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-10DOI: 10.19087/jveteriner.2024.25.1.93
Stephanie Ariella Gunawan, I. Suartha, N. S. Dharmawan
Atopic dermatitis is a common skin disorder in dogs. This study was aimed to determine the effect of eco enzyme on dogs suffering from atopic dermatitis in terms of changes in total erythrocytes, hemoglobin levels, hematocrit values and erythrocyte indices. Two groups of dogs with atopic dermatitis were used in this study: concentration A (three dogs) were bathed with 10% concentration of eco enzyme at intervals of three days, and concentration B (two dogs) were bathed with 10% concentration of eco enzyme at intervals of three days, then continued a week later with eco enzyme concentration of 2% at intervals once a week. Both groups were given treatment for five weeks. Hematology data were analyzed through tiered pattern analysis of variance followed by Duncan’s multiple range test using SPSS which showed that the concentration of eco enzyme and the duration of observation (weeks) had no significant effect on total erythrocytes, hemoglobin, hematocrit, MCH and MCHC, although they influenced MCV. Also, there was a real interaction between the concentration of eco enzyme and the duration of observation at MCH. It can be concluded that therapy with eco enzyme has not been able to improve total erythrocytes, hemoglobin, hematocrit, MCH and MCHC, but has been able to improve MCV in dogs with atopic dermatitis.
{"title":"Total Eritrosit, Kadar Hemoglobin, Nilai Hematokrit, dan Indeks Eritrosit Anjing Penderita Dermatitis Atopik Pascaterapi dengan Eco Enzyme","authors":"Stephanie Ariella Gunawan, I. Suartha, N. S. Dharmawan","doi":"10.19087/jveteriner.2024.25.1.93","DOIUrl":"https://doi.org/10.19087/jveteriner.2024.25.1.93","url":null,"abstract":"Atopic dermatitis is a common skin disorder in dogs. This study was aimed to determine the effect of eco enzyme on dogs suffering from atopic dermatitis in terms of changes in total erythrocytes, hemoglobin levels, hematocrit values and erythrocyte indices. Two groups of dogs with atopic dermatitis were used in this study: concentration A (three dogs) were bathed with 10% concentration of eco enzyme at intervals of three days, and concentration B (two dogs) were bathed with 10% concentration of eco enzyme at intervals of three days, then continued a week later with eco enzyme concentration of 2% at intervals once a week. Both groups were given treatment for five weeks. Hematology data were analyzed through tiered pattern analysis of variance followed by Duncan’s multiple range test using SPSS which showed that the concentration of eco enzyme and the duration of observation (weeks) had no significant effect on total erythrocytes, hemoglobin, hematocrit, MCH and MCHC, although they influenced MCV. Also, there was a real interaction between the concentration of eco enzyme and the duration of observation at MCH. It can be concluded that therapy with eco enzyme has not been able to improve total erythrocytes, hemoglobin, hematocrit, MCH and MCHC, but has been able to improve MCV in dogs with atopic dermatitis.","PeriodicalId":17749,"journal":{"name":"Jurnal Veteriner","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141362250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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