Molecular frequency of bovine leukemia virus in Creole cattle of Eastern Colombia

IF 1.9 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Veterinary and Animal Science Pub Date : 2024-06-12 DOI:10.1016/j.vas.2024.100372
Jeiczon Jaimes-Dueñez, Eyner Goyeneche-Ortiz, Marisol Tique-Oviedo, Melissa C Ortiz-Pineda, Luis Cardenas-Pinto, Angela Patricia Jimenez-Leaño, Julian Ruiz-Saenz
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Abstract

Enzootic Bovine Leukosis (EBL), caused by the bovine leukosis virus (BLV), is a global infectious disease affecting livestock. This study focuses on studying the frequency and genetic traits of BLV in three Creole breeds including Chino Santandereano (Chino), Casanareño (CAS), and Sanmartinero (SM) in Eastern Colombia. We implemented a cross-sectional survey between 2019 and 2020 across four departments (Arauca, Casanare, Santander and Meta) in Eastern Colombia to assess the molecular characteristics of BLV infection in these breeds. A total of 253 cattle were analyzed, of which 42.6 %, 28.8 %, and 28.4 % belonged to the Chino, CAS, and SM breeds, respectively. BLV provirus was detected using nested polymerase chain reaction (n-PCR) targeting the conserved region of the env viral gene. Subsequently, the obtained amplicons were sequenced and subjected to phylogenetic analyses. The overall BLV infection frequency was 26.48 % (95 % CI: 21.01 – 31.98 %), with Chino exhibiting the highest frequency (35.1 %) following by SAM and CAS, respectively (P < 0.05). Other epidemiological variables associated with the infection included age, department, and season (P < 0.05). BLV-positive animals exhibited elevated levels of total serum proteins (P < 0.05), while molecular characterization revealed the exclusive circulation of BLV genotype 1 within these breeds. This study provides an updated assessment of BLV infection in Creole breeds from the eastern of Colombia, underscoring their lower infection frequency compared to introduced breeds and their reduced susceptibility to developing clinical signs. The epidemiological and molecular characteristics observed should be considered in developing control programs aimed at improving genetic resistance to BLV in Colombian cattle.

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哥伦比亚东部克里奥尔牛中牛白血病病毒的分子频率
由牛白血病病毒(BLV)引起的牛白血病(EBL)是一种影响家畜的全球性传染病。本研究重点研究了哥伦比亚东部三个克里奥尔品种(包括奇诺-桑坦德雷亚诺(Chino Santandereano)、卡萨纳雷尼奥(Casanareño)和圣马蒂内罗(Sanmartinero))中BLV的发生频率和遗传特征。我们于 2019 年至 2020 年期间在哥伦比亚东部的四个省(阿劳卡省、卡萨纳雷省、桑坦德省和梅塔省)开展了一项横断面调查,以评估这些品种感染 BLV 的分子特征。共分析了 253 头牛,其中 42.6%、28.8% 和 28.4% 分别属于奇诺、CAS 和 SM 品种。使用巢式聚合酶链反应(n-PCR)检测BLV前病毒,目标是env病毒基因的保守区。随后,对获得的扩增子进行了测序和系统发育分析。BLV的总体感染率为26.48%(95% CI:21.01 - 31.98%),奇诺的感染率最高(35.1%),其次分别是萨姆和中科院(P < 0.05)。与感染有关的其他流行病学变量包括年龄、部门和季节(P < 0.05)。BLV阳性动物的血清总蛋白水平升高(P <0.05),而分子特征显示,BLV基因型1在这些品种中独家流行。这项研究对哥伦比亚东部克里奥尔种猪的 BLV 感染情况进行了最新评估,强调与引进种猪相比,克里奥尔种猪的感染频率较低,而且出现临床症状的易感性也较低。在制定旨在提高哥伦比亚牛群对 BLV 遗传抗性的控制计划时,应考虑观察到的流行病学和分子特征。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Veterinary and Animal Science
Veterinary and Animal Science Veterinary-Veterinary (all)
CiteScore
3.50
自引率
0.00%
发文量
43
审稿时长
47 days
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