Identification and evaluation of an endophytic antagonistic yeast for the control of gray mold (Botrytis cinerea) in apple and mechanisms of action

IF 4.5 1区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Food microbiology Pub Date : 2024-06-07 DOI:10.1016/j.fm.2024.104583
Xiaoyan Yu , Kuan Zhang , Jiayi Liu , Zhenhua Zhao , Bowen Guo , Xiangjing Wang , Wensheng Xiang , Junwei Zhao
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Abstract

Gray mold, caused by Botrytis cinerea, is a prevalent postharvest disease of apple that limits their shelf life, resulting in significant economic losses. The use of antagonistic microorganisms has been shown to be an effective approach for managing postharvest diseases of fruit. In the present study, an endophytic yeast strain PGY-2 was isolated from apples and evaluated for its biocontrol efficacy against gray mold and its mechanisms of action. Results indicated that strain PGY-2, identified as Bullera alba, reduced the occurrence of gray mold on apples and significantly inhibited lesion development in pathogen-inoculated wounds. Gray mold control increased with the use of increasing concentrations of PGY-2, with the best disease control observed at 108 cells/mL. Notably, Bullera alba PGY-2 did not inhibit the growth of Botrytis cinerea in vitro indicating that the yeast antagonist did not produce antimicrobial compounds. The rapid colonization and stable population of PGY-2 in apple wounds at 4 °C and 25 °C confirmed its ability to compete with pathogens for nutrients and space. PGY-2 also had a strong ability to form a biofilm and enhanced the activity of multiple defense-related enzymes (POD, PPO, APX, SOD, PAL) in host tissues. Our study is the first time to report the use of Bullera alba PGY-2 as a biocontrol agent for postharvest diseases of apple and provide evidence that Bullera alba PGY-2 represents an endophytic antagonistic yeast with promising biocontrol potential and alternative to the use of synthetic, chemical fungicides for the control of postharvest gray mold in apples.

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鉴定和评估用于控制苹果灰霉病(Botrytis cinerea)的内生拮抗酵母菌及其作用机制
由灰霉病菌引起的灰霉病是苹果采后的一种普遍病害,它限制了苹果的货架期,造成了巨大的经济损失。使用拮抗微生物已被证明是管理水果采后病害的有效方法。本研究从苹果中分离出了内生酵母菌株 PGY-2,并评估了其对灰霉病的生物防治效果及其作用机制。结果表明,被鉴定为 Bullera alba 的菌株 PGY-2 能减少苹果上灰霉病的发生,并显著抑制病原体接种伤口的病变发展。随着 PGY-2 浓度的增加,灰霉病的控制效果也随之增加,108 个细胞/毫升时的病害控制效果最好。值得注意的是,Bullera alba PGY-2 在体外并不抑制灰霉病菌的生长,这表明酵母拮抗剂并不产生抗菌化合物。PGY-2 在 4 °C 和 25 °C 温度条件下在苹果伤口中快速定殖并形成稳定种群,这证明它有能力与病原体竞争养分和空间。PGY-2 还具有很强的形成生物膜的能力,并能增强宿主组织中多种防御相关酶(POD、PPO、APX、SOD、PAL)的活性。我们的研究首次报道了将白毫银针酵母 PGY-2 用作苹果采后病害的生物防治剂,并提供证据证明白毫银针酵母 PGY-2 是一种内生拮抗酵母,具有良好的生物防治潜力,可替代使用合成化学杀菌剂防治苹果采后灰霉病。
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来源期刊
Food microbiology
Food microbiology 工程技术-生物工程与应用微生物
CiteScore
11.30
自引率
3.80%
发文量
179
审稿时长
44 days
期刊介绍: Food Microbiology publishes original research articles, short communications, review papers, letters, news items and book reviews dealing with all aspects of the microbiology of foods. The editors aim to publish manuscripts of the highest quality which are both relevant and applicable to the broad field covered by the journal. Studies must be novel, have a clear connection to food microbiology, and be of general interest to the international community of food microbiologists. The editors make every effort to ensure rapid and fair reviews, resulting in timely publication of accepted manuscripts.
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