Open-source and low-cost miniature microscope for on-site fluorescence detection

IF 2.1 Q3 ENGINEERING, ELECTRICAL & ELECTRONIC HardwareX Pub Date : 2024-06-15 DOI:10.1016/j.ohx.2024.e00545

Michio Kawai , Haruka Oda , Hisatoshi Mimura , Toshihisa Osaki , Shoji Takeuchi

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Abstract

The development of a compact and affordable fluorescence microscope can be a formidable challenge for growing needs in on-site testing and detection of fluorescent labeled biological systems, especially for those who specialize in biology rather than in engineering. In response to such a situation, we present an open-source miniature fluorescence microscope using Raspberry Pi. Our fluorescence microscope, with dimensions of 19.2 × 13.6 × 8.2 cm³ (including the display, computer, light-blocking case, and other operational requirements), not only offers cost-effectiveness (costing less than $500) but is also highly customizable to meet specific application needs. The 12.3-megapixel Raspberry Pi HQ Camera captures high-resolution imagery, while the equipped wide-angle lens provides a field of view measuring 21 × 15 mm². The integrated wireless LAN in the Raspberry Pi, along with software-controllable high-powered fluorescence LEDs, holds potential for a wide range of applications. This open-source fluorescence microscope offers biohybrid sensor developers a versatile tool to streamline unfamiliar mechanical design tasks and open new opportunities for on-site fluorescence detections.

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用于现场荧光检测的开源低成本微型显微镜

为了满足日益增长的现场测试和检测荧光标记生物系统的需求，特别是对于那些专门从事生物学而非工程学研究的人员来说，开发一种结构紧凑、价格低廉的荧光显微镜是一项艰巨的挑战。针对这种情况，我们提出了一种使用 Raspberry Pi 的开源微型荧光显微镜。我们的荧光显微镜尺寸为 19.2 × 13.6 × 8.2 立方厘米（包括显示屏、计算机、遮光箱和其他操作要求），不仅具有成本效益（成本不到 500 美元），而且可高度定制，以满足特定的应用需求。1230 万像素的 Raspberry Pi HQ 摄像机可捕捉高分辨率图像，配备的广角镜头可提供 21 × 15 平方毫米的视野。Raspberry Pi 中集成的无线局域网以及软件可控的高功率荧光 LED 具有广泛的应用潜力。这种开源荧光显微镜为生物杂交传感器开发人员提供了一种多功能工具，可简化不熟悉的机械设计任务，并为现场荧光检测带来新的机遇。

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来源期刊

HardwareX Engineering-Industrial and Manufacturing Engineering

CiteScore

4.10

自引率

18.20%

发文量

124

审稿时长

24 weeks

期刊介绍： HardwareX is an open access journal established to promote free and open source designing, building and customizing of scientific infrastructure (hardware). HardwareX aims to recognize researchers for the time and effort in developing scientific infrastructure while providing end-users with sufficient information to replicate and validate the advances presented. HardwareX is open to input from all scientific, technological and medical disciplines. Scientific infrastructure will be interpreted in the broadest sense. Including hardware modifications to existing infrastructure, sensors and tools that perform measurements and other functions outside of the traditional lab setting (such as wearables, air/water quality sensors, and low cost alternatives to existing tools), and the creation of wholly new tools for either standard or novel laboratory tasks. Authors are encouraged to submit hardware developments that address all aspects of science, not only the final measurement, for example, enhancements in sample preparation and handling, user safety, and quality control. The use of distributed digital manufacturing strategies (e.g. 3-D printing) is encouraged. All designs must be submitted under an open hardware license.