Establishment of a rabbit model of different degrees of testicular torsion

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Abstract

Introduction and objectives

Different degrees of testicular torsion result in varying degrees of testicular damage, which influences treatment options and outcomes. Therefore, establishing a testicular torsion model with different degrees is necessary for clinical diagnosis.

Materials and methods

Rabbits were randomly divided into four groups and their spermatic cords were twisted at 0 °, 180 °, 360 °, and 720 °, respectively. Color Doppler flow imaging (CDFI) were performed to evaluate the blood supply in testicles. The twisted testicles were surgically removed at six hours post-operation and were evaluated by morphological observation and Hematoxylin and Eosin staining.

Results

CDFI signals were gradually decreased as the degree of testicular torsion increased, and scores of CDFI in the 360 ° and 720 ° groups were significantly decreased at postoperative six hours compared to pre-surgery. Compared to the sham, the testicle in the 180 ° group exhibited slight congestion, whereas the testicles in the 360 ° and 720 ° groups were dark red in color and had severe congestion and unrecognizable vessels. Hematoxylin and Eosin staining showed mild spermatogenic cell reduction and testicular interstitial hemorrhage in the 180 ° group. In the 360 ° and 720 ° groups, disordered seminiferous tubules, shed spermatogenic cells in tubules, inflammatory cell infiltration, and severe hemorrhage were found. In comparison with the sham, interstitial hemorrhage scores in the 360 ° and 720 ° groups were significantly higher, and scores of germinal epithelial cell thickness in the three testicular torsion groups were significantly decreased.

Conclusions

Collectively, we successfully constructed a testicular torsion model with different degrees in rabbits.
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建立不同程度睾丸扭转的兔子模型
简介和目的不同程度的睾丸扭转会导致不同程度的睾丸损伤,从而影响治疗方案和效果。因此,建立不同程度的睾丸扭转模型对临床诊断十分必要。材料和方法将兔子随机分为四组,分别将其精索扭转 0°、180°、360° 和 720°。进行彩色多普勒血流成像(CDFI)以评估睾丸的血液供应情况。结果 随着睾丸扭转程度的增加,CDFI信号逐渐减弱,360°和720°组的CDFI评分在术后6小时明显低于术前。与假体相比,180°组的睾丸表现出轻微充血,而360°和720°组的睾丸呈暗红色,充血严重,血管无法辨认。血色素和伊红染色显示 180 °组有轻微的生精细胞减少和睾丸间质出血。在 360 ° 和 720 ° 组中,发现曲细精管紊乱、精管内生精细胞脱落、炎性细胞浸润和严重出血。与假体相比,360 °组和 720 °组的间质出血评分明显升高,三个睾丸扭转组的生精上皮细胞厚度评分明显降低。
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