Silver nanoparticles stabilized by chitosan-polyvinyl alcohol polymers mediated by Pistacia extract for treatment of functional dyspepsia and gastric cancer

IF 6.7 3区 材料科学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY Journal of Science: Advanced Materials and Devices Pub Date : 2024-06-08 DOI:10.1016/j.jsamd.2024.100757
Cui Jin , Zhongxin Zhang , Xiaopei Li
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Abstract

The impact of the nanoparticles on the dyspepsia mice gut microbiota is evaluated through the utilization of the 16S rRNA technique. Various methods were employed to characterize the NPs. In the textile, culinary, paper, leather, and printing sectors, synthetic dyes are frequently employed as coloring agents. Because these dye molecules are harmful to both the environment and living things, it is difficult to remove them sustainably. Here, we have produced nanoparticles embedded in chitosan-polyvinyl alcohol hydrogel (CS-PVA) by using bark's Pistacia under ultrasonic irradiation. The reduction of Ag + ions into Ag0 NPs was detected by a visual change in the colors. UV–Vis analysis indicated that the characteristic SPR band appeared near ∼440–450 nm. UV/Vis, EDX-elemental mapping, SEM, TEM, ICP-OES, and XRD analysis were applied to characterize the Ag NPs/CS-PVA nanocomposite. The recent study focused on the cellular and molecular aspects. The MTT assay was conducted for 48 h to evaluate the anti-human gastric cancer and cytotoxicity efficacies of the treated cells with nanocomposite. The assessment was performed on normal (HUVEC) and gastric cancer cells, namely MKN45 and NCI–N87. The IC50 values of nanocomposite against MKN45 and NCI–N87 were 180 and 151 μg/mL, respectively. The dose-dependent reduction in malignant gastric cell viability was observed in the nanocomposite presence. Furthermore, the nanocomposite induced cell apoptosis by 40–50%, leading to an increase in the expression of pro-apoptotic markers (Bax and cleaved caspase-8) and a decrease in the expression of the anti-apoptotic marker, Bcl-2. Furthermore, the nanocomposite showed inhibitory effects on colony formation. Our results indicate that the nanocomposite can increase p53 expression and decrease the expression of STAT3 in treated cells. This indicates that p53 and STAT3 play crucial roles in the biological effects induced by the extract in human gastric carcinoma cells. The data from the gut microbiome suggested that nanocomposites have the ability to control the rebalancing of homeostasis and microbiome composition by decreasing the levels of harmful bacteria, and at the same time, increasing the levels of beneficial bacteria. Following the introduction of nanocomposite, there was an observed rise in the levels of gastrointestinal hormones in the serum, including gastrin and motilin, while the levels of vasoactive intestinal peptide decreased. The application of nanocomposite led to an improvement in gastrointestinal movement, encompassing a rise in the rate of transit through the small intestine and the rate of gastric emptying. These findings offer that the Ag NPs/CS-PVA nanocomposite could be a promising anticancer treatment for functional dyspepsia and gastric cancer.

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由壳聚糖-聚乙烯醇聚合物稳定的银纳米粒子,以楷模提取物为介质,用于治疗功能性消化不良和胃癌
利用 16S rRNA 技术评估了纳米粒子对消化不良小鼠肠道微生物群的影响。采用了多种方法对纳米粒子进行表征。在纺织、烹饪、造纸、皮革和印刷行业,合成染料经常被用作着色剂。由于这些染料分子对环境和生物都有害,因此很难可持续地去除它们。在此,我们利用树皮中的楷属植物,在超声波照射下制备了嵌入壳聚糖-聚乙烯醇水凝胶(CS-PVA)中的纳米粒子。Ag + 离子还原成 Ag0 NPs 的过程是通过颜色的视觉变化来检测的。紫外可见光分析表明,特征 SPR 波段出现在 ∼440-450 nm 附近。紫外可见光谱、EDX 元素图谱、扫描电子显微镜、电子显微镜、ICP-OES 和 XRD 分析被用于表征 Ag NPs/CS-PVA 纳米复合材料。最近的研究侧重于细胞和分子方面。为了评估纳米复合材料处理细胞的抗人胃癌和细胞毒性效果,对细胞进行了 48 小时的 MTT 试验。评估对象是正常细胞(HUVEC)和胃癌细胞,即 MKN45 和 NCI-N87。纳米复合材料对 MKN45 和 NCI-N87 的 IC50 值分别为 180 和 151 μg/mL。在纳米复合材料存在的情况下,恶性胃细胞存活率的降低呈剂量依赖性。此外,纳米复合材料可诱导细胞凋亡 40-50%,导致促凋亡标志物(Bax 和裂解的 caspase-8)的表达增加,而抗凋亡标志物 Bcl-2 的表达减少。此外,纳米复合材料还对集落形成有抑制作用。我们的研究结果表明,纳米复合材料能增加处理细胞中 p53 的表达,降低 STAT3 的表达。这表明 p53 和 STAT3 在提取物诱导的人胃癌细胞生物效应中起着关键作用。来自肠道微生物组的数据表明,纳米复合材料有能力通过降低有害细菌的水平,同时提高有益细菌的水平来控制平衡和微生物组组成的再平衡。引入纳米复合材料后,观察到血清中胃肠激素(包括胃泌素和胃动素)的水平上升,而血管活性肠肽的水平下降。纳米复合材料的应用改善了胃肠道运动,包括提高了小肠转运率和胃排空率。这些研究结果表明,Ag NPs/CS-PVA 纳米复合材料可能是治疗功能性消化不良和胃癌的一种很有前景的抗癌疗法。
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来源期刊
Journal of Science: Advanced Materials and Devices
Journal of Science: Advanced Materials and Devices Materials Science-Electronic, Optical and Magnetic Materials
CiteScore
11.90
自引率
2.50%
发文量
88
审稿时长
47 days
期刊介绍: In 1985, the Journal of Science was founded as a platform for publishing national and international research papers across various disciplines, including natural sciences, technology, social sciences, and humanities. Over the years, the journal has experienced remarkable growth in terms of quality, size, and scope. Today, it encompasses a diverse range of publications dedicated to academic research. Considering the rapid expansion of materials science, we are pleased to introduce the Journal of Science: Advanced Materials and Devices. This new addition to our journal series offers researchers an exciting opportunity to publish their work on all aspects of materials science and technology within the esteemed Journal of Science. With this development, we aim to revolutionize the way research in materials science is expressed and organized, further strengthening our commitment to promoting outstanding research across various scientific and technological fields.
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