Advances in cryo-ET data processing: meeting the demands of visual proteomics

IF 6.1 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Current opinion in structural biology Pub Date : 2024-06-17 DOI:10.1016/j.sbi.2024.102861

Abigail J.I. Watson , Alberto Bartesaghi

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Abstract

Cryogenic electron tomography (cryo-ET), a method that enables the viewing of biomolecules in near-native environments at high resolution, is rising in accessibility and applicability. Over the past several years, once slow sample preparation and data collection procedures have seen innovations which enable rapid collection of the large datasets required for attaining high resolution structures. Increased data availability has provided a driving force for exciting improvements in cryo-ET data processing methodologies throughout the entire processing pipeline and the development of accessible graphical user interfaces (GUIs) that enable individuals inexperienced in computational fields to convert raw tilt series into 3D structures. These advances in data processing are enabling cryo-ET to attain higher resolution and extending its applicability to more complex samples.

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低温电子显微镜数据处理的进展：满足视觉蛋白质组学的需求。

低温电子断层扫描（cryo-ET）是一种能够在近原生环境中以高分辨率观察生物分子的方法，其可获得性和适用性正在不断提高。在过去的几年中，曾经缓慢的样品制备和数据收集程序有了创新，能够快速收集获得高分辨率结构所需的大量数据集。数据可用性的提高推动了整个处理流程中低温电子显微镜数据处理方法的重大改进，也推动了图形用户界面（GUI）的发展，使缺乏计算领域经验的人也能将原始倾斜序列转换成三维结构。数据处理方面的这些进步使低温电子显微镜能够达到更高的分辨率，并将其适用范围扩展到更复杂的样本。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current opinion in structural biology 生物-生化与分子生物学

CiteScore

12.20

自引率

2.90%

发文量

179

审稿时长

6-12 weeks

期刊介绍： Current Opinion in Structural Biology (COSB) aims to stimulate scientifically grounded, interdisciplinary, multi-scale debate and exchange of ideas. It contains polished, concise and timely reviews and opinions, with particular emphasis on those articles published in the past two years. In addition to describing recent trends, the authors are encouraged to give their subjective opinion of the topics discussed. In COSB, we help the reader by providing in a systematic manner: 1. The views of experts on current advances in their field in a clear and readable form. 2. Evaluations of the most interesting papers, annotated by experts, from the great wealth of original publications. [...] The subject of Structural Biology is divided into twelve themed sections, each of which is reviewed once a year. Each issue contains two sections, and the amount of space devoted to each section is related to its importance. -Folding and Binding- Nucleic acids and their protein complexes- Macromolecular Machines- Theory and Simulation- Sequences and Topology- New constructs and expression of proteins- Membranes- Engineering and Design- Carbohydrate-protein interactions and glycosylation- Biophysical and molecular biological methods- Multi-protein assemblies in signalling- Catalysis and Regulation