Zhibo Hu , Haishan Cui , Qiang Wang , Cheng Li , Senhua Chen , Zhizeng Gao , Lan Liu , Bo Peng , Jing Li
{"title":"Induced production of defensive secondary metabolites from Aspergillus fumigatiaffinis by co-culture with Aspergillus alabamensis","authors":"Zhibo Hu , Haishan Cui , Qiang Wang , Cheng Li , Senhua Chen , Zhizeng Gao , Lan Liu , Bo Peng , Jing Li","doi":"10.1016/j.phytochem.2024.114187","DOIUrl":null,"url":null,"abstract":"<div><p>Seven previously undescribed compounds, including four diketomorpholine alkaloids (<strong>1</strong>‒<strong>4</strong>), one indole diketopiperazine alkaloid (<strong>9</strong>), one chromone (<strong>10</strong>), and one benzoic acid derivative (<strong>13</strong>), and nine known compounds (<strong>5</strong>–<strong>8</strong>, <strong>11</strong>, <strong>12</strong>, and <strong>14</strong>–<strong>16</strong>) were isolated from two different fungal sources. Nine of these metabolites (<strong>1</strong>–<strong>9</strong>) were obtained from a seagrass-derived <em>Aspergillus alabamensis</em> SYSU-6778, while the others were obtained from a mixed culture of <em>A. alabamensis</em> SYSU-6778 and a co-isolated fungus <em>A</em>. <em>fumigatiaffinis</em> SYSU-6786. The chemical structures of the compounds were deduced via spectroscopic techniques (including HRESIMS, 1D and 2D NMR), chemical reactions, and ECD calculations. It is worth noting that compound <strong>10</strong> was identified as a defensive secondary metabolite of strain SYSU-6786, produced through the induction of compound <strong>8</strong> under co-culture conditions. Compounds <strong>3</strong> and <strong>4</strong> possessed a naturally rare isotryptophan core. Moreover, compounds <strong>1</strong> and <strong>2</strong> exhibited potent inhibitory activities against fish pathogenic bacterium <em>Edwardsiella ictalurid,</em> with minimum inhibitory concentration values of 10.0 μg/mL for both compounds.</p></div>","PeriodicalId":20170,"journal":{"name":"Phytochemistry","volume":null,"pages":null},"PeriodicalIF":3.2000,"publicationDate":"2024-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Phytochemistry","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0031942224002243","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Seven previously undescribed compounds, including four diketomorpholine alkaloids (1‒4), one indole diketopiperazine alkaloid (9), one chromone (10), and one benzoic acid derivative (13), and nine known compounds (5–8, 11, 12, and 14–16) were isolated from two different fungal sources. Nine of these metabolites (1–9) were obtained from a seagrass-derived Aspergillus alabamensis SYSU-6778, while the others were obtained from a mixed culture of A. alabamensis SYSU-6778 and a co-isolated fungus A. fumigatiaffinis SYSU-6786. The chemical structures of the compounds were deduced via spectroscopic techniques (including HRESIMS, 1D and 2D NMR), chemical reactions, and ECD calculations. It is worth noting that compound 10 was identified as a defensive secondary metabolite of strain SYSU-6786, produced through the induction of compound 8 under co-culture conditions. Compounds 3 and 4 possessed a naturally rare isotryptophan core. Moreover, compounds 1 and 2 exhibited potent inhibitory activities against fish pathogenic bacterium Edwardsiella ictalurid, with minimum inhibitory concentration values of 10.0 μg/mL for both compounds.
期刊介绍:
Phytochemistry is a leading international journal publishing studies of plant chemistry, biochemistry, molecular biology and genetics, structure and bioactivities of phytochemicals, including ''-omics'' and bioinformatics/computational biology approaches. Phytochemistry is a primary source for papers dealing with phytochemicals, especially reports concerning their biosynthesis, regulation, and biological properties both in planta and as bioactive principles. Articles are published online as soon as possible as Articles-in-Press and in 12 volumes per year. Occasional topic-focussed special issues are published composed of papers from invited authors.