Climate change and plant rhizosphere microbiomes: an experiential course-embedded research project.

IF 1.6 Q2 EDUCATION, SCIENTIFIC DISCIPLINES Journal of Microbiology & Biology Education Pub Date : 2024-08-29 Epub Date: 2024-06-18 DOI:10.1128/jmbe.00046-24
James A Parejko
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Abstract

The current and ongoing challenges brought on by climate change will require future scientists who have hands-on experience using advanced molecular techniques, can work with large data sets, and can make correlations between metadata and microbial diversity. A course-embedded research project can prepare students to answer complex research questions that might help plants adapt to climate change. The project described herein uses plants as a host to study the impact of climate change-induced drought on host-microbe interactions through next-generation DNA sequencing and analysis using a command-line program. Specifically, the project studies the impact of simulated drought on the rhizosphere microbiome of Fast Plants rapid cycling Brassica rapa using inexpensive greenhouse supplies and 16S rRNA V3/V4 Illumina sequencing. Data analysis is performed with the freely accessible Python-based microbiome bioinformatics platform QIIME 2.

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气候变化与植物根瘤微生物群:体验式课程嵌入研究项目。
气候变化带来的当前和持续挑战需要未来的科学家拥有使用先进分子技术的实践经验,能够处理大型数据集,并能将元数据与微生物多样性联系起来。课程中的研究项目可以帮助学生回答复杂的研究问题,从而帮助植物适应气候变化。本文介绍的项目以植物为宿主,通过使用命令行程序进行下一代 DNA 测序和分析,研究气候变化引起的干旱对宿主与微生物相互作用的影响。具体来说,该项目利用廉价的温室用品和 16S rRNA V3/V4 Illumina 测序技术,研究模拟干旱对快速植物快速循环 Brassica rapa 根瘤微生物组的影响。数据分析使用可免费访问的基于 Python- 的微生物组生物信息学平台 QIIME 2 进行。
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来源期刊
Journal of Microbiology & Biology Education
Journal of Microbiology & Biology Education EDUCATION, SCIENTIFIC DISCIPLINES-
CiteScore
3.00
自引率
26.30%
发文量
95
审稿时长
22 weeks
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