miR-221-3p as a potential biomarker of chronic periodontitis and its regulatory effect on inflammatory response.

IF 0.9 4区 医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE American journal of dentistry Pub Date : 2024-06-01
Jiajun Qi, Qianqian Gui, Weizhong Lu, Chao Meng, Mei Liu
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引用次数: 0

Abstract

Purpose: To explore the function of miR-221-3p in the development and course of chronic periodontitis (CP) and offer a fresh avenue for CP diagnosis and management.

Methods: miR-221-3p expression was detected by RT-qPCR. The clinical diagnostic value of miR-221-3p in CP patients was analyzed by receiver operating characteristic (ROC). ELISA was used to determine the IL-1β and IL-6 in CP subjects and healthy controls. Pearson correlation analysis was performed with miR-221-3p. PDLCs were induced by LPS, transfected with miR-221-3p mimics, and their expression was analyzed for the effects of IL-1β, and IL-6.

Results: The miR-221-3p expression was lower in the gingival sulcus fluid GCF of CP subjects compared to healthy controls. miR-221-3p showed high potential for clinical diagnosis in CP patients by ROC analysis, with high specificity and sensitivity. miR-221-3p was negatively correlated with Probing pocket depth (PD), Attachment loss (AL), Plaque index (PI), and Bleeding index (BI), and negatively correlated with inflammatory factors IL-1β and IL-6. In LPS-induced PDLCs, IL-1β and IL-6 were significantly increased, whereas miR-221-3p was significantly downregulated. Overexpression of miR-221-3p inhibited the production of inflammatory factors IL-1β and IL-6 in LPS-induced PDLCs.

Clinical significance: miR-221-3p expression may be a potential biological marker for the diagnosis of chronic periodontitis and provide a new direction for its treatment of chronic periodontitis.

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作为慢性牙周炎潜在生物标志物的 miR-221-3p 及其对炎症反应的调节作用
目的:探讨miR-221-3p在慢性牙周炎(CP)的发生和发展过程中的功能,为CP的诊断和治疗提供新的途径。方法:采用 RT-qPCR 法检测 miR-221-3p 的表达,用接收器操作特征(ROC)分析 miR-221-3p 在 CP 患者中的临床诊断价值。用酶联免疫吸附法测定 CP 受试者和健康对照组的 IL-1β 和 IL-6。与 miR-221-3p 进行了皮尔逊相关分析。用LPS诱导PDLCs,转染miR-221-3p模拟物,分析其表达对IL-1β和IL-6的影响:miR-221-3p与探袋深度(PD)、附着丧失(AL)、斑块指数(PI)和出血指数(BI)呈负相关,与炎症因子IL-1β和IL-6呈负相关。在 LPS 诱导的 PDLCs 中,IL-1β 和 IL-6 显著增加,而 miR-221-3p 则显著下调。临床意义:miR-221-3p 的表达可能是诊断慢性牙周炎的潜在生物学标志物,并为慢性牙周炎的治疗提供了新的方向。
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来源期刊
American journal of dentistry
American journal of dentistry 医学-牙科与口腔外科
CiteScore
2.40
自引率
7.10%
发文量
57
审稿时长
1 months
期刊介绍: The American Journal of Dentistry, published by Mosher & Linder, Inc., provides peer-reviewed scientific articles with clinical significance for the general dental practitioner.
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