In Vitro Human Endometrial Cells and In Vivo Rat Model Studies Suggest That Ulipristal Acetate Impacts Endometrial Compatibility for Embryo Implantation

IF 4.7 3区医学 Q1 MEDICINE, RESEARCH & EXPERIMENTAL Archives of Medical Research Pub Date : 2024-06-19 DOI:10.1016/j.arcmed.2024.103026

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Abstract

Background

Ulipristal acetate (UPA) and levonorgestrel are used as emergency hormonal contraceptives. Although both are highly effective in preventing pregnancy, UPA shows efficacy even when taken up to 120 h after unprotected sexual intercourse.

Aims

To investigate whether the mechanism of UPA's contraceptive action involves post-fertilization effects.

Methods

In vitro and in vivo studies using cultured human endometrial cells and a pre-clinical rat model.

Results

Endometrial cells treated with UPA showed changes in the expression of receptivity gene markers and a significant decrease in trophoblast spheroids attached to the cultured cells. In addition, administration of UPA to female unmated rats decreased the expression of implantation-related genes in the endometrium and inhibited the number of implantation sites in the mated group compared to the non-treated group.

Conclusions

These results support that UPA as an emergency contraceptive might have post-fertilization effects that may affect embryo implantation.

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体外人类子宫内膜细胞和体内大鼠模型研究表明，醋酸乌利司他会影响子宫内膜与胚胎着床的兼容性。

背景：醋酸乌利司他（UPA）和左炔诺孕酮被用作紧急激素避孕药。目的：研究 UPA 的避孕作用机制是否涉及受精后效应：方法：使用培养的人类子宫内膜细胞和临床前大鼠模型进行体外和体内研究：结果：经 UPA 处理的子宫内膜细胞的受孕基因标志物的表达发生了变化，附着在培养细胞上的滋养细胞球明显减少。此外，与未处理组相比，给未交配的雌性大鼠服用 UPA 可降低子宫内膜植入相关基因的表达，并抑制交配组植入点的数量：这些结果证明，作为一种紧急避孕药，UPA可能会产生受精后效应，从而影响胚胎着床。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Archives of Medical Research 医学-医学：研究与实验

CiteScore

12.50

自引率

0.00%

发文量

审稿时长

28 days

期刊介绍： Archives of Medical Research serves as a platform for publishing original peer-reviewed medical research, aiming to bridge gaps created by medical specialization. The journal covers three main categories - biomedical, clinical, and epidemiological contributions, along with review articles and preliminary communications. With an international scope, it presents the study of diseases from diverse perspectives, offering the medical community original investigations ranging from molecular biology to clinical epidemiology in a single publication.