A novel “AND” logic platform based on ICT and FRET for constructing near-infrared dual-locked fluorescence probe

IF 4.1 3区工程技术 Q2 CHEMISTRY, APPLIED Dyes and Pigments Pub Date : 2024-06-18 DOI:10.1016/j.dyepig.2024.112292

Jian Qiang, Zefeng Han, Ziwei Guo, Yanru Wang, Yajing Li, Sheng Lu, Fang Wang, Xiaoqiang Chen

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Abstract

In this study, a new “AND” logic platform based on intramolecular charge transfer (ICT) and Förster resonance energy transfer (FRET) is developed for constructing a near-infrared dual-locked fluorescence probe with high resistance to pH in biological environments. Using esterase and glutathione (GSH) as representative activators, the developed dual-locked probe BBQ650-SS-ClHC-E discriminates cancer cells from normal cells. The esterase substrate in the probe blocks the ICT process, while the GSH substrate acts as a linker to connect the fluorescence part with the quencher part to induce the FRET process. The fluorescence activation at 732 nm operates as an “AND” logic that depends on the “ICT-on” and “FRET-off” states in the presence of both esterase and GSH. BBQ650-SS-ClHC-E can successfully discriminate HeLa, 4T1, and RAW264.7 cancer cells from HFL1 normal cells and 4T1 tumor-bearing cells in living mice. This new platform can be used to develop dual-locked fluorescence probes.

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基于信息通信技术和 FRET 的新型 "AND "逻辑平台，用于构建近红外双锁定荧光探针

本研究开发了一种基于分子内电荷转移（ICT）和佛斯特共振能量转移（FRET）的新型 "AND "逻辑平台，用于构建一种在生物环境中具有高抗 pH 值能力的近红外双锁定荧光探针。以酯酶和谷胱甘肽（GSH）为代表性激活剂，所开发的双锁定探针 BBQ650-SS-ClHC-E 可以区分癌细胞和正常细胞。探针中的酯酶底物阻断了 ICT 过程，而 GSH 底物则作为连接物将荧光部分与淬灭剂部分连接起来，诱导 FRET 过程。在酯酶和 GSH 同时存在的情况下，732 纳米波长处的荧光激活是一个 "AND "逻辑，取决于 "ICT-on "和 "FRET-off "两种状态。BBQ650-SS-ClHC-E 能在活体小鼠体内成功地将 HeLa、4T1 和 RAW264.7 癌细胞与 HFL1 正常细胞和 4T1 肿瘤细胞区分开来。这一新平台可用于开发双锁定荧光探针。

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来源期刊

Dyes and Pigments 工程技术-材料科学：纺织

CiteScore

8.20

自引率

13.30%

发文量

933

审稿时长

33 days

期刊介绍： Dyes and Pigments covers the scientific and technical aspects of the chemistry and physics of dyes, pigments and their intermediates. Emphasis is placed on the properties of the colouring matters themselves rather than on their applications or the system in which they may be applied. Thus the journal accepts research and review papers on the synthesis of dyes, pigments and intermediates, their physical or chemical properties, e.g. spectroscopic, surface, solution or solid state characteristics, the physical aspects of their preparation, e.g. precipitation, nucleation and growth, crystal formation, liquid crystalline characteristics, their photochemical, ecological or biological properties and the relationship between colour and chemical constitution. However, papers are considered which deal with the more fundamental aspects of colourant application and of the interactions of colourants with substrates or media. The journal will interest a wide variety of workers in a range of disciplines whose work involves dyes, pigments and their intermediates, and provides a platform for investigators with common interests but diverse fields of activity such as cosmetics, reprographics, dye and pigment synthesis, medical research, polymers, etc.