Design and Application of Microfluidic Capture Device for Physical-Magnetic Isolation of MCF-7 Circulating Tumor Cells.

IF 4.9 3区 工程技术 Q1 CHEMISTRY, ANALYTICAL Biosensors-Basel Pub Date : 2024-06-15 DOI:10.3390/bios14060308
Akhilesh Bendre, Derangula Somasekhara, Varalakshmi K Nadumane, Ganesan Sriram, Ramesh S Bilimagga, Mahaveer D Kurkuri
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Abstract

Circulating tumor cells (CTCs) are a type of cancer cell that spreads from the main tumor to the bloodstream, and they are often the most important among the various entities that can be isolated from the blood. For the diagnosis of cancer, conventional biopsies are often invasive and unreliable, whereas a liquid biopsy, which isolates the affected item from blood or lymph fluid, is a less invasive and effective diagnostic technique. Microfluidic technologies offer a suitable channel for conducting liquid biopsies, and this technology is utilized to extract CTCs in a microfluidic chip by physical and bio-affinity-based techniques. This effort uses functionalized magnetic nanoparticles (MNPs) in a unique microfluidic chip to collect CTCs using a hybrid (physical and bio-affinity-based/guided magnetic) capturing approach with a high capture rate. Accordingly, folic acid-functionalized Fe3O4 nanoparticles have been used to capture MCF-7 (breast cancer) CTCs with capture efficiencies reaching up to 95% at a 10 µL/min flow rate. Moreover, studies have been conducted to support this claim, including simulation and biomimetic investigations.

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用于 MCF-7 循环肿瘤细胞物理磁分离的微流控捕获装置的设计与应用
循环肿瘤细胞(CTCs)是一种从主要肿瘤扩散到血液中的癌细胞,它们往往是从血液中分离出来的各种实体中最重要的一种。在癌症诊断中,传统的活组织检查往往具有创伤性且不可靠,而从血液或淋巴液中分离出受影响项目的液体活组织检查则是一种创伤性较小且有效的诊断技术。微流控技术为进行液体活检提供了一个合适的渠道,利用这种技术,可以通过物理和生物亲和技术在微流控芯片中提取 CTC。这项研究在独特的微流体芯片中使用功能化磁性纳米粒子(MNPs),通过混合(物理和生物亲和性/导向磁性)捕获方法收集 CTC,捕获率很高。因此,叶酸功能化的 Fe3O4 纳米粒子已被用于捕获 MCF-7(乳腺癌)CTC,在 10 µL/min 的流速下捕获效率高达 95%。此外,还进行了包括模拟和生物仿真调查在内的研究来支持这一说法。
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来源期刊
Biosensors-Basel
Biosensors-Basel Biochemistry, Genetics and Molecular Biology-Clinical Biochemistry
CiteScore
6.60
自引率
14.80%
发文量
983
审稿时长
11 weeks
期刊介绍: Biosensors (ISSN 2079-6374) provides an advanced forum for studies related to the science and technology of biosensors and biosensing. It publishes original research papers, comprehensive reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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