The potential role of liquid-liquid phase separation in the cellular fate of the compartments for unconventional protein secretion.

IF 4.5 3区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Protein Science Pub Date : 2024-07-01 DOI:10.1002/pro.5085
Luis Felipe S Mendes, Carolina O Gimenes, Marília D O da Silva, Saroj K Rout, Roland Riek, Antonio J Costa-Filho
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Abstract

Eukaryotic cells have developed intricate mechanisms for biomolecule transport, particularly in stressful conditions. This interdisciplinary study delves into unconventional protein secretion (UPS) pathways activated during starvation, facilitating the export of proteins bypassing most of the components of the classical secretory machinery. Specifically, we focus on the underexplored mechanisms of the GRASP's role in UPS, particularly in biogenesis and cargo recruitment for the vesicular-like compartment for UPS. Our results show that liquid-liquid phase separation (LLPS) plays a key role in the coacervation of Grh1, the GRASP yeast homologue, under starvation-like conditions. This association seems a precursor to the Compartment for Unconventional Protein Secretion (CUPS) biogenesis. Grh1's self-association is regulated by electrostatic, hydrophobic, and hydrogen-bonding interactions. Importantly, our study demonstrates that phase-separated states of Grh1 can recruit UPS cargo under starvation-like situations. Additionally, we explore how the coacervate liquid-to-solid transition could impact cells' ability to return to normal post-stress states. Our findings offer insights into intracellular protein dynamics and cell adaptive responses to stress.

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液-液相分离在非常规蛋白质分泌区细胞命运中的潜在作用。
真核细胞发展出了复杂的生物大分子运输机制,尤其是在压力条件下。这项跨学科研究深入探讨了在饥饿状态下激活的非常规蛋白质分泌(UPS)途径,这种途径绕过了经典分泌机制的大部分组件,促进了蛋白质的输出。具体而言,我们重点研究了 GRASP 在 UPS 中尚未被充分探索的作用机制,尤其是在 UPS 的囊状区室的生物生成和货物招募方面。我们的研究结果表明,在类似饥饿的条件下,液-液相分离(LLPS)在Grh1(GRASP的酵母同源物)的凝聚过程中发挥了关键作用。这种结合似乎是非常规蛋白分泌区(CUPS)生物发生的前体。Grh1 的自我结合受静电、疏水和氢键相互作用的调控。重要的是,我们的研究表明,在类似饥饿的情况下,相分离状态的 Grh1 可以招募 UPS 货物。此外,我们还探讨了凝聚态液态到固态的转变如何影响细胞恢复正常应激后状态的能力。我们的研究结果为细胞内蛋白质动力学和细胞对应激的适应性反应提供了见解。
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来源期刊
Protein Science
Protein Science 生物-生化与分子生物学
CiteScore
12.40
自引率
1.20%
发文量
246
审稿时长
1 months
期刊介绍: Protein Science, the flagship journal of The Protein Society, is a publication that focuses on advancing fundamental knowledge in the field of protein molecules. The journal welcomes original reports and review articles that contribute to our understanding of protein function, structure, folding, design, and evolution. Additionally, Protein Science encourages papers that explore the applications of protein science in various areas such as therapeutics, protein-based biomaterials, bionanotechnology, synthetic biology, and bioelectronics. The journal accepts manuscript submissions in any suitable format for review, with the requirement of converting the manuscript to journal-style format only upon acceptance for publication. Protein Science is indexed and abstracted in numerous databases, including the Agricultural & Environmental Science Database (ProQuest), Biological Science Database (ProQuest), CAS: Chemical Abstracts Service (ACS), Embase (Elsevier), Health & Medical Collection (ProQuest), Health Research Premium Collection (ProQuest), Materials Science & Engineering Database (ProQuest), MEDLINE/PubMed (NLM), Natural Science Collection (ProQuest), and SciTech Premium Collection (ProQuest).
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