Detecting emergence of ruptures in individual layers of the stretched intestinal wall using optical coherence elastography: A pilot study

IF 2 3区 物理与天体物理 Q3 BIOCHEMICAL RESEARCH METHODS Journal of Biophotonics Pub Date : 2024-06-25 DOI:10.1002/jbio.202400086
Elena B. Kiseleva, Alexander A. Sovetsky, Maksim G. Ryabkov, Ekaterina V. Gubarkova, Anton A. Plekhanov, Evgeniya L. Bederina, Arseniy L. Potapov, Alexandra Y. Bogomolova, Vladimir Y. Zaitsev, Natalia D. Gladkova
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Abstract

We report a new application of compression optical coherence elastography (C-OCE) to monitor the emergence of ruptures in individual layers of longitudinally stretched small-intestine walls using tissue samples (n = 36) from nine minipigs. Before stretching, C-OCE successfully estimated stiffness for each intestine-wall layer: longitudinal muscular layer with serosa, circumferential muscular layer, submucosa and mucosa. In stretched samples, C-OCE clearly visualized initial stiffening in both muscular layers. By 25% elongation, a sharp stiffness decrease for the longitudinal muscular layer, indicated emergence of tears in all samples. With further stretching, for most samples, ruptures emerged in the circumferential muscular layer and submucosa, while mucosa remained undamaged. Histology confirmed the OCE-revealed damaging and absence of tissue damage for ~15% elongation. Thus, C-OCE has demonstrated a high potential for determining the safety tissue-stretching threshold which afterward may be used intraoperatively to prevent rupture risk in intestinal tissues stretched during various diagnostic/therapeutic procedures.

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利用光学相干弹性成像技术检测拉伸肠壁各层出现的破裂:试点研究。
我们报告了一种压缩光学相干弹性成像(C-OCE)的新应用,它利用九只小猪的组织样本(n = 36)监测纵向拉伸的小肠壁各层出现破裂的情况。在拉伸前,C-OCE成功地估算出了每一层肠壁的硬度:带浆膜的纵向肌肉层、周向肌肉层、粘膜下层和粘膜。在拉伸的样本中,C-OCE 可以清楚地看到两个肌肉层的初始僵化。拉伸到 25% 时,纵向肌肉层的硬度急剧下降,表明所有样本都出现了撕裂。进一步拉伸后,大多数样本的周向肌肉层和粘膜下层出现破裂,而粘膜则未受损。组织学检查证实了 OCE 揭示的损伤和约 15% 的拉伸无组织损伤。因此,C-OCE 在确定安全组织拉伸阈值方面具有很大的潜力,之后可用于术中防止在各种诊断/治疗过程中拉伸的肠组织出现破裂风险。
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来源期刊
Journal of Biophotonics
Journal of Biophotonics 生物-生化研究方法
CiteScore
5.70
自引率
7.10%
发文量
248
审稿时长
1 months
期刊介绍: The first international journal dedicated to publishing reviews and original articles from this exciting field, the Journal of Biophotonics covers the broad range of research on interactions between light and biological material. The journal offers a platform where the physicist communicates with the biologist and where the clinical practitioner learns about the latest tools for the diagnosis of diseases. As such, the journal is highly interdisciplinary, publishing cutting edge research in the fields of life sciences, medicine, physics, chemistry, and engineering. The coverage extends from fundamental research to specific developments, while also including the latest applications.
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