Kunhong Xiao, Li Li, Yang Chen, Rong Lin, Boyuan Wen, Zhiqiang Wang, Yan Huang
Vision impairment caused by diabetic retinopathy (DR) is often irreversible, making early‐stage diagnosis imperative. Raman spectroscopy emerges as a powerful tool, capable of providing molecular fingerprints of tissues. This study employs RS to detect ex vivo retinal tissue from diabetic rats at various stages of the disease. Transmission electron microscopy was utilized to reveal the ultrastructural changes in retinal tissue. Following spectral preprocessing of the acquired data, the random forest and orthogonal partial least squares‐discriminant analysis algorithms were employed for spectral data analysis. The entirety of Raman spectra and all annotated bands accurately and distinctly differentiate all animal groups, and can identify significant molecules from the spectral data. Bands at 524, 1335, 543, and 435 cm−1 were found to be associated with the preproliferative phase of DR. Bands at 1045 and 1335 cm−1 were found to be associated with early stages of DR.
糖尿病视网膜病变（DR）造成的视力损害往往是不可逆的，因此早期诊断势在必行。拉曼光谱是一种强大的工具，能够提供组织的分子指纹。本研究利用拉曼光谱检测糖尿病大鼠不同阶段的体外视网膜组织。透射电子显微镜用于揭示视网膜组织的超微结构变化。对获取的数据进行光谱预处理后，采用随机森林和正交偏最小二乘判别分析算法进行光谱数据分析。整个拉曼光谱和所有注释波段都能准确、清晰地区分所有动物组别，并能从光谱数据中识别出重要的分子。研究发现，524、1335、543 和 435 cm-1 处的条带与 DR 的增殖前阶段有关。1045 和 1335 cm-1 处的波段与 DR 的早期阶段有关。
{"title":"Diagnostic application in streptozotocin‐induced diabetic retinopathy rats: A study based on Raman spectroscopy and machine learning","authors":"Kunhong Xiao, Li Li, Yang Chen, Rong Lin, Boyuan Wen, Zhiqiang Wang, Yan Huang","doi":"10.1002/jbio.202400115","DOIUrl":"https://doi.org/10.1002/jbio.202400115","url":null,"abstract":"Vision impairment caused by diabetic retinopathy (DR) is often irreversible, making early‐stage diagnosis imperative. Raman spectroscopy emerges as a powerful tool, capable of providing molecular fingerprints of tissues. This study employs RS to detect ex vivo retinal tissue from diabetic rats at various stages of the disease. Transmission electron microscopy was utilized to reveal the ultrastructural changes in retinal tissue. Following spectral preprocessing of the acquired data, the random forest and orthogonal partial least squares‐discriminant analysis algorithms were employed for spectral data analysis. The entirety of Raman spectra and all annotated bands accurately and distinctly differentiate all animal groups, and can identify significant molecules from the spectral data. Bands at 524, 1335, 543, and 435 cm<jats:sup>−1</jats:sup> were found to be associated with the preproliferative phase of DR. Bands at 1045 and 1335 cm<jats:sup>−1</jats:sup> were found to be associated with early stages of DR.<jats:inline-graphic xmlns:xlink=\"http://www.w3.org/1999/xlink\" xlink:href=\"graphic/jbio202400115-gra-0001.png\" xlink:title=\"image\"/>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141517079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Zhang, V. R. Besaga, P. Rühl, C. Zou, S. H. Heinemann, Y. Wang, F. Setzpfandt
This study addresses the critical need for high signal‐to‐noise ratio in optical detection methods for biological sample discrimination under low‐photon‐flux conditions to ensure accuracy without compromising sample integrity. We explore polarization‐based probing, which often excels over intensity modulation when assessing a specimen's morphology. Leveraging non‐classical light sources, our approach capitalizes on sub‐Poissonian photon statistics and quantum correlation‐based measurements. We present a novel, highly sensitive method for probing single‐layer cell cultures using entangled photon pairs. Our approach demonstrates potential for monolayer cell analysis, distinguishing between two types of monolayer cells and their host medium. The experimental results highlight our method's sensitivity, showcasing its potential for biological sample detection using quantum techniques, and paving the way for advanced diagnostic methodologies.
{"title":"Probing polarization response of monolayer cell cultures with entangled photon pairs","authors":"L. Zhang, V. R. Besaga, P. Rühl, C. Zou, S. H. Heinemann, Y. Wang, F. Setzpfandt","doi":"10.1002/jbio.202400018","DOIUrl":"https://doi.org/10.1002/jbio.202400018","url":null,"abstract":"This study addresses the critical need for high signal‐to‐noise ratio in optical detection methods for biological sample discrimination under low‐photon‐flux conditions to ensure accuracy without compromising sample integrity. We explore polarization‐based probing, which often excels over intensity modulation when assessing a specimen's morphology. Leveraging non‐classical light sources, our approach capitalizes on sub‐Poissonian photon statistics and quantum correlation‐based measurements. We present a novel, highly sensitive method for probing single‐layer cell cultures using entangled photon pairs. Our approach demonstrates potential for monolayer cell analysis, distinguishing between two types of monolayer cells and their host medium. The experimental results highlight our method's sensitivity, showcasing its potential for biological sample detection using quantum techniques, and paving the way for advanced diagnostic methodologies.<jats:inline-graphic xmlns:xlink=\"http://www.w3.org/1999/xlink\" xlink:href=\"graphic/jbio202400018-gra-0001.png\" xlink:title=\"image\"/>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141517080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Kalampouka, R. R. Mould, S. W. Botchway, A. M. Mackenzie, A. V. Nunn, E. L. Thomas, J. D. Bell
Photobiomodulation, utilising non‐ionising light in the visible and near‐infrared (NIR) spectrum, has been suggested as a potential method for enhancing tissue repair, reducing inflammation and possibly mitigating cancer‐therapy‐associated side effects. NIR light is suggested to be absorbed intracellularly, mainly by chromophores within the mitochondria. This study examines the impact of 734 nm NIR light on cellular senescence. Cancer (MCF7 and A549) and non‐cancer (MCF10A and IMR‐90) cell populations were subjected to 63 mJ/cm2 NIR‐light exposure for 6 days. Senescence levels were quantified by measuring active senescence‐associated beta‐galactosidase. Exposure to NIR light significantly increases senescence levels in cancer (10.0%–203.2%) but not in non‐cancer cells (p > 0.05). Changes in senescence were associated with significant modulation of mitochondrial homeostasis, including increased levels of reactive oxygen species (p < 0.05) and mitochondrial membrane potential (p < 0.05) post‐NIR‐light treatment. These results suggest that NIR light modulates cellular chemistry, arresting the proliferation of cancer cells via senescence induction while sparing non‐cancer cells.
{"title":"Selective induction of senescence in cancer cells through near‐infrared light treatment via mitochondrial modulation","authors":"I. Kalampouka, R. R. Mould, S. W. Botchway, A. M. Mackenzie, A. V. Nunn, E. L. Thomas, J. D. Bell","doi":"10.1002/jbio.202400046","DOIUrl":"https://doi.org/10.1002/jbio.202400046","url":null,"abstract":"Photobiomodulation, utilising non‐ionising light in the visible and near‐infrared (NIR) spectrum, has been suggested as a potential method for enhancing tissue repair, reducing inflammation and possibly mitigating cancer‐therapy‐associated side effects. NIR light is suggested to be absorbed intracellularly, mainly by chromophores within the mitochondria. This study examines the impact of 734 nm NIR light on cellular senescence. Cancer (MCF7 and A549) and non‐cancer (MCF10A and IMR‐90) cell populations were subjected to 63 mJ/cm<jats:sup>2</jats:sup> NIR‐light exposure for 6 days. Senescence levels were quantified by measuring active senescence‐associated beta‐galactosidase. Exposure to NIR light significantly increases senescence levels in cancer (10.0%–203.2%) but not in non‐cancer cells (<jats:italic>p</jats:italic> > 0.05). Changes in senescence were associated with significant modulation of mitochondrial homeostasis, including increased levels of reactive oxygen species (<jats:italic>p</jats:italic> < 0.05) and mitochondrial membrane potential (<jats:italic>p</jats:italic> < 0.05) post‐NIR‐light treatment. These results suggest that NIR light modulates cellular chemistry, arresting the proliferation of cancer cells via senescence induction while sparing non‐cancer cells.<jats:inline-graphic xmlns:xlink=\"http://www.w3.org/1999/xlink\" xlink:href=\"graphic/jbio202400046-gra-0001.png\" xlink:title=\"image\"/>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141501959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yulia M. Alexandrovskaya, Ekaterina M. Kasianenko, Alexander A. Sovetsky, Alexander L. Matveyev, Dmitry A. Atyakshin, Olga I. Patsap, Mikhail A. Ignatiuk, Artem V. Volodkin, Vladimir Y. Zaitsev
Optical coherence elastography (OCE) demonstrated impressive abilities for diagnosing tissue types/states using differences in their biomechanics. Usually, OCE visualizes tissue deformation induced by some additional stimulus (e.g., contact compression or auxiliary elastic‐wave excitation). We propose a new variant of OCE with osmotically induced straining (OIS‐OCE) and demonstrate its application to assess various stages of proteoglycan content degradation in cartilage. The information‐bearing signatures in OIS‐OCE are the magnitude and rate of strains caused by the application of osmotically active solutions onto the sample surface. OCE examination of the induced strains does not require special tissue preparation, the osmotic stimulation is highly reproducible, and strains are observed in noncontact mode. Several minutes suffice to obtain a conclusion. These features are promising for intraoperative method usage when express assessment of tissue state is required during surgical operations. The “waterfall” images demonstrate the development of cumulative osmotic strains in control and degraded cartilage samples.
{"title":"Optical coherence elastography with osmotically induced strains: Preliminary demonstration for express detection of cartilage degradation","authors":"Yulia M. Alexandrovskaya, Ekaterina M. Kasianenko, Alexander A. Sovetsky, Alexander L. Matveyev, Dmitry A. Atyakshin, Olga I. Patsap, Mikhail A. Ignatiuk, Artem V. Volodkin, Vladimir Y. Zaitsev","doi":"10.1002/jbio.202400016","DOIUrl":"https://doi.org/10.1002/jbio.202400016","url":null,"abstract":"Optical coherence elastography (OCE) demonstrated impressive abilities for diagnosing tissue types/states using differences in their biomechanics. Usually, OCE visualizes tissue deformation induced by some additional stimulus (e.g., contact compression or auxiliary elastic‐wave excitation). We propose a new variant of OCE with osmotically induced straining (OIS‐OCE) and demonstrate its application to assess various stages of proteoglycan content degradation in cartilage. The information‐bearing signatures in OIS‐OCE are the magnitude and rate of strains caused by the application of osmotically active solutions onto the sample surface. OCE examination of the induced strains does not require special tissue preparation, the osmotic stimulation is highly reproducible, and strains are observed in noncontact mode. Several minutes suffice to obtain a conclusion. These features are promising for intraoperative method usage when express assessment of tissue state is required during surgical operations. The “waterfall” images demonstrate the development of cumulative osmotic strains in control and degraded cartilage samples.<jats:inline-graphic xmlns:xlink=\"http://www.w3.org/1999/xlink\" xlink:href=\"graphic/jbio202400016-gra-0001.png\" xlink:title=\"image\"/>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140826859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Marco Lombardo, Sebastiano Serrao, Giuseppe Massimo Bernava, Giuseppe Lombardo
Seven human donor eye globes underwent corneal cross‐linking using theranostic UV‐A device with accessory corneal iontophoresis system for patterned delivery of a 0.22% riboflavin solution. Theranostic‐guided UV‐A light illumination assessed riboflavin distribution and treated corneas at 10 mW/cm2 for 9 min with a 5.0‐mm beam size. Corneal topography maps were taken at baseline and 2‐h post‐treatment. Analysis utilized corneal topography elevation data, with results showing controlled riboflavin delivery led to a consistent gradient, with 40% higher levels centrally (248 ± 79 μg/cm3) than peripherally (180 ± 72 μg/cm3 at ±2.5 mm from the center). Theranostic‐guided UV‐A light irradiation resulted in significant changes in corneal topography, with a decrease in best‐fit sphere value (−0.7 ± 0.2 D; p < 0.001) and consistent downward shift in corneal elevation map (−11.7 ± 3.7 μm). The coefficient of variation was 2.5%, indicating high procedure performance in achieving significant and reliable corneal flattening.
{"title":"Spatial targeted delivery of riboflavin with a controlled corneal iontophoresis delivery system in theranostic‐guided UV‐A light photo‐therapy","authors":"Marco Lombardo, Sebastiano Serrao, Giuseppe Massimo Bernava, Giuseppe Lombardo","doi":"10.1002/jbio.202400068","DOIUrl":"https://doi.org/10.1002/jbio.202400068","url":null,"abstract":"Seven human donor eye globes underwent corneal cross‐linking using theranostic UV‐A device with accessory corneal iontophoresis system for patterned delivery of a 0.22% riboflavin solution. Theranostic‐guided UV‐A light illumination assessed riboflavin distribution and treated corneas at 10 mW/cm<jats:sup>2</jats:sup> for 9 min with a 5.0‐mm beam size. Corneal topography maps were taken at baseline and 2‐h post‐treatment. Analysis utilized corneal topography elevation data, with results showing controlled riboflavin delivery led to a consistent gradient, with 40% higher levels centrally (248 ± 79 μg/cm<jats:sup>3</jats:sup>) than peripherally (180 ± 72 μg/cm<jats:sup>3</jats:sup> at ±2.5 mm from the center). Theranostic‐guided UV‐A light irradiation resulted in significant changes in corneal topography, with a decrease in best‐fit sphere value (−0.7 ± 0.2 D; <jats:italic>p</jats:italic> < 0.001) and consistent downward shift in corneal elevation map (−11.7 ± 3.7 μm). The coefficient of variation was 2.5%, indicating high procedure performance in achieving significant and reliable corneal flattening.<jats:inline-graphic xmlns:xlink=\"http://www.w3.org/1999/xlink\" xlink:href=\"graphic/jbio202400068-gra-0005.png\" xlink:title=\"image\"/>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140827088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Vascular diseases are a leading cause of death and disability worldwide. Despite having precursor conditions like peripheral arterial disease (PAD), they are often only diagnosed after the onset of stroke or heart attack. Low‐cost, portable, noninvasive, point‐of‐care (POC), label‐free assessment of deep vascular function benefits PAD diagnosis, especially in resource poor settings of the world. Doppler ultrasound‐based blood flow measurements can diagnose PAD, albeit with limited sensitivity and specificity. To overcome this, here, we propose the first‐of‐its‐kind dual‐modality photoacoustic‐and‐ultrasound (PAUS) imaging system that integrates a multiwavelength pulsed laser diode (PLD) with a compact ultrasound data acquisition unit. The mesoscopic imaging depth of the portable PLD‐PAUS system was validated using tissue phantoms, and its multispectral photoacoustic imaging capabilities were validated using an atherosclerosis‐mimicking phantom. Furthermore, we demonstrated high‐contrast volumetric in vivo photoacoustic imaging of rodent abdominal vasculature and quantified vessel reactivity due to hypercapnia stimulation. The multiparametric functional and molecular imaging capabilities of the PLD‐PAUS system holds promise for POC applications.
血管疾病是导致全球死亡和残疾的主要原因。尽管有外周动脉疾病（PAD）等前驱疾病，但这些疾病往往在中风或心脏病发作后才被诊断出来。对深层血管功能进行低成本、便携式、无创、护理点（POC）、无标记的评估，有利于 PAD 诊断，尤其是在全球资源匮乏的环境中。基于多普勒超声的血流测量可诊断出急性心肌梗死，但灵敏度和特异性有限。为了克服这一问题，我们在此提出了首创的光声和超声（PAUS）双模态成像系统，该系统集成了多波长脉冲激光二极管（PLD）和紧凑型超声数据采集单元。使用组织模型验证了便携式 PLD-PAUS 系统的中观成像深度，并使用动脉粥样硬化模拟模型验证了其多光谱光声成像能力。此外，我们还展示了啮齿类动物腹部血管的高对比度体积光声成像，并量化了高碳酸血症刺激引起的血管反应性。PLD-PAUS 系统的多参数功能和分子成像能力为 POC 应用带来了希望。
{"title":"Multiwavelength laser diode based portable photoacoustic and ultrasound imaging system for point of care applications","authors":"Mahaan Mitra, Abigail Haworth, Prameth Gaddale, Faisal Badran, Nuzhath Lagno, Colette Pameijer, Faisal Aziz, Sri‐Rajasekhar Kothapalli","doi":"10.1002/jbio.202400058","DOIUrl":"https://doi.org/10.1002/jbio.202400058","url":null,"abstract":"Vascular diseases are a leading cause of death and disability worldwide. Despite having precursor conditions like peripheral arterial disease (PAD), they are often only diagnosed after the onset of stroke or heart attack. Low‐cost, portable, noninvasive, point‐of‐care (POC), label‐free assessment of deep vascular function benefits PAD diagnosis, especially in resource poor settings of the world. Doppler ultrasound‐based blood flow measurements can diagnose PAD, albeit with limited sensitivity and specificity. To overcome this, here, we propose the first‐of‐its‐kind dual‐modality photoacoustic‐and‐ultrasound (PAUS) imaging system that integrates a multiwavelength pulsed laser diode (PLD) with a compact ultrasound data acquisition unit. The mesoscopic imaging depth of the portable PLD‐PAUS system was validated using tissue phantoms, and its multispectral photoacoustic imaging capabilities were validated using an atherosclerosis‐mimicking phantom. Furthermore, we demonstrated high‐contrast volumetric in vivo photoacoustic imaging of rodent abdominal vasculature and quantified vessel reactivity due to hypercapnia stimulation. The multiparametric functional and molecular imaging capabilities of the PLD‐PAUS system holds promise for POC applications.<jats:inline-graphic xmlns:xlink=\"http://www.w3.org/1999/xlink\" xlink:href=\"graphic/jbio202400058-gra-0001.png\" xlink:title=\"image\"/>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140826854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Marcelo S Nogueira, Jacqueline E Gunther, K. Komolibus, S. Ryan, Brian S Murray, Moises Jezzini, Declan Kennedy, S. Andersson-Engels
As a rapidly growing field, biophotonics demonstrates an increasingly higher demand for interdisciplinary professionals and requires the implementation of a structured approach to educational and outreach activities focused on appropriate curriculum, and teaching and learning for audiences with diverse technical backgrounds and learning styles. Our study shows the main findings upon applying this approach to biophotonics workshops delivered 2 consecutive years while updating and improving learning outcomes, teaching strategies, workshop content based on student and teacher feedback. We provided resources for a variety of lecture-based, experimental, computer simulation activities. Quality of subject matter, teaching, and overall learning was rated as "Very good" or "Good" by 88%, 76%, and 82% of students in average, respectively. Application of our teaching strategies and materials during short- and long-term workshops/courses could potentially increase the interest in pursuing careers in the biophotonics field and related areas, leading to standardized approaches in designing education and outreach events across centers.
{"title":"Teaching and learning in biophotonics: Crossing the bridge between educators and students.","authors":"Marcelo S Nogueira, Jacqueline E Gunther, K. Komolibus, S. Ryan, Brian S Murray, Moises Jezzini, Declan Kennedy, S. Andersson-Engels","doi":"10.1002/jbio.202300491","DOIUrl":"https://doi.org/10.1002/jbio.202300491","url":null,"abstract":"As a rapidly growing field, biophotonics demonstrates an increasingly higher demand for interdisciplinary professionals and requires the implementation of a structured approach to educational and outreach activities focused on appropriate curriculum, and teaching and learning for audiences with diverse technical backgrounds and learning styles. Our study shows the main findings upon applying this approach to biophotonics workshops delivered 2 consecutive years while updating and improving learning outcomes, teaching strategies, workshop content based on student and teacher feedback. We provided resources for a variety of lecture-based, experimental, computer simulation activities. Quality of subject matter, teaching, and overall learning was rated as \"Very good\" or \"Good\" by 88%, 76%, and 82% of students in average, respectively. Application of our teaching strategies and materials during short- and long-term workshops/courses could potentially increase the interest in pursuing careers in the biophotonics field and related areas, leading to standardized approaches in designing education and outreach events across centers.","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140655128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Sharan, G. Khouqeer, B. A. El-Badry, Anup M. Upadhyaya
This research presents a comprehensive study focused on the design, implementation, and analysis of an innovative fiber Bragg grating (FBG) based foot pressure assessment system. FBG sensors strategically placed on the great toe, metatarsal 1, metatarsal 2, and heel provided distinct peak resonant wavelengths, strains, and pressures during experimental cycles. Participant 1 exhibited peak resonant wavelength of 1537.745 nm for great toe, 1537.792 nm for metatarsal 1, 1537.812 nm for metatarsal 2, and 1537.824 nm for heel. Participant 2 showcased distinct graphical representations with peak resonant wavelengths ranging from 1537.903 to 1537.917 nm. In a fracture patient condition, the FBG-based system monitored weight-bearing capacity, integrated with real-time X-ray imaging for dynamic insights of rehabilitation as distinct approach. The strains and pressures at each position exhibited notable variations along with the sensitivity of 1.31με obtained across all positions, underscoring the FBG-based system's reliability in capturing subtle foot pressure.
{"title":"Design and analysis of a fiber Bragg grating-based foot pressure assessment system.","authors":"P. Sharan, G. Khouqeer, B. A. El-Badry, Anup M. Upadhyaya","doi":"10.1002/jbio.202400070","DOIUrl":"https://doi.org/10.1002/jbio.202400070","url":null,"abstract":"This research presents a comprehensive study focused on the design, implementation, and analysis of an innovative fiber Bragg grating (FBG) based foot pressure assessment system. FBG sensors strategically placed on the great toe, metatarsal 1, metatarsal 2, and heel provided distinct peak resonant wavelengths, strains, and pressures during experimental cycles. Participant 1 exhibited peak resonant wavelength of 1537.745 nm for great toe, 1537.792 nm for metatarsal 1, 1537.812 nm for metatarsal 2, and 1537.824 nm for heel. Participant 2 showcased distinct graphical representations with peak resonant wavelengths ranging from 1537.903 to 1537.917 nm. In a fracture patient condition, the FBG-based system monitored weight-bearing capacity, integrated with real-time X-ray imaging for dynamic insights of rehabilitation as distinct approach. The strains and pressures at each position exhibited notable variations along with the sensitivity of 1.31με obtained across all positions, underscoring the FBG-based system's reliability in capturing subtle foot pressure.","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140655628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Focal damage due to stroke causes widespread abnormal changes in brain function and hemispheric asymmetry. In this study, functional near-infrared spectroscopy (fNIRS) was used to collect resting-state hemoglobin data from 85 patients with subacute stroke and 26 healthy controls, to comparatively analyze the characteristics of lateralization after stroke in terms of cortical activity, functional networks, and hemodynamic lags. Higher intensity of motor cortical activity, lower hemispheric autonomy, and more abnormal hemodynamic leads or lags were found in the affected hemisphere. Lateralization metrics of the three aspects were all associated with the Fugl-Meyer score. The results of this study prove that three lateralization metrics may provide clinical reference for stroke rehabilitation. Meanwhile, the present study piloted the use of resting-state fNIRS for analyzing hemodynamic lag, demonstrating the potential of fNIRS to assess hemodynamic abnormalities in addition to the study of cortical neurological function after stroke.
{"title":"Lateralization of cortical activity, networks, and hemodynamic lag after stroke: A resting-state fNIRS study.","authors":"Gongcheng Xu, Tiandi Chen, Jiahui Yin, Guangjian Shao, Yubo Fan, Zengyong Li","doi":"10.1002/jbio.202400012","DOIUrl":"https://doi.org/10.1002/jbio.202400012","url":null,"abstract":"Focal damage due to stroke causes widespread abnormal changes in brain function and hemispheric asymmetry. In this study, functional near-infrared spectroscopy (fNIRS) was used to collect resting-state hemoglobin data from 85 patients with subacute stroke and 26 healthy controls, to comparatively analyze the characteristics of lateralization after stroke in terms of cortical activity, functional networks, and hemodynamic lags. Higher intensity of motor cortical activity, lower hemispheric autonomy, and more abnormal hemodynamic leads or lags were found in the affected hemisphere. Lateralization metrics of the three aspects were all associated with the Fugl-Meyer score. The results of this study prove that three lateralization metrics may provide clinical reference for stroke rehabilitation. Meanwhile, the present study piloted the use of resting-state fNIRS for analyzing hemodynamic lag, demonstrating the potential of fNIRS to assess hemodynamic abnormalities in addition to the study of cortical neurological function after stroke.","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140665395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This paper introduces a spectral analysis method for monitoring the human skin in vivo based on a combination of terahertz time-domain spectroscopy (THz-TDS) and optical coherence tomography (OCT). The method can quantitatively measure the refractive index, thickness and transmission coefficient of epidermis, and the refractive index of dermis in natural, as well as the tension condition of the skin. An optically reflective model for the multilayer structure of the skin is first established. The initial thickness of the epidermis is then measured by OCT as a known quantity for the established model. By fitting the established model to the experimentally obtained THz-TDS signals, the above parameters of the skin can be calibrated. Furthermore, the dependence of these skin parameters on the tension status are investigated. This study provides a means for terahertz technology to measure the skin in vivo.
本文介绍了一种基于太赫兹时域光谱（THz-TDS）和光学相干断层扫描（OCT）相结合的活体皮肤监测光谱分析方法。该方法可定量测量表皮的折射率、厚度和透射系数，以及真皮的折射率和皮肤的张力状况。首先建立了皮肤多层结构的光学反射模型。然后用 OCT 测量表皮的初始厚度，作为已建立模型的已知量。通过将建立的模型与实验获得的 THz-TDS 信号进行拟合，可以校准皮肤的上述参数。此外，还研究了这些皮肤参数与张力状态的关系。这项研究为太赫兹技术测量体内皮肤提供了一种方法。
{"title":"Characterization of human skin under tension using terahertz time domain spectroscopy","authors":"Baitong Chen, Pengyan Zhang, Keyu Tan, Hengxin Zhu, Zixuan Li, Chuanwei Li, Zhiyong Wang","doi":"10.1002/jbio.202400003","DOIUrl":"10.1002/jbio.202400003","url":null,"abstract":"<p>This paper introduces a spectral analysis method for monitoring the human skin in vivo based on a combination of terahertz time-domain spectroscopy (THz-TDS) and optical coherence tomography (OCT). The method can quantitatively measure the refractive index, thickness and transmission coefficient of epidermis, and the refractive index of dermis in natural, as well as the tension condition of the skin. An optically reflective model for the multilayer structure of the skin is first established. The initial thickness of the epidermis is then measured by OCT as a known quantity for the established model. By fitting the established model to the experimentally obtained THz-TDS signals, the above parameters of the skin can be calibrated. Furthermore, the dependence of these skin parameters on the tension status are investigated. This study provides a means for terahertz technology to measure the skin in vivo.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140670030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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