Adrian Sanchez-Fernandez*, Jia-Fei Poon, Anna Elizabeth Leung, Sylvain François Prévost and Cedric Dicko,
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引用次数: 0
Abstract
Proteins are adjustable units from which biomaterials with designed properties can be developed. However, non-native folded states with controlled topologies are hardly accessible in aqueous environments, limiting their prospects as building blocks. Here, we demonstrate the ability of a series of anhydrous deep eutectic solvents (DESs) to precisely control the conformational landscape of proteins. We reveal that systematic variations in the chemical composition of binary and ternary DESs dictate the stabilization of a wide range of conformations, that is, compact globular folds, intermediate folding states, or unfolded chains, as well as controlling their collective behavior. Besides, different conformational states can be visited by simply adjusting the composition of ternary DESs, allowing for the refolding of unfolded states and vice versa. Notably, we show that these intermediates can trigger the formation of supramolecular gels, also known as eutectogels, where their mechanical properties correlate to the folding state of the protein. Given the inherent vulnerability of proteins outside the native fold in aqueous environments, our findings highlight DESs as tailorable solvents capable of stabilizing various non-native conformations on demand through solvent design.
蛋白质是一种可调节的单元,可据此开发出具有设计特性的生物材料。然而,具有可控拓扑结构的非原生折叠态在水性环境中很难获得,这限制了它们作为构件的前景。在这里,我们展示了一系列无水深共晶溶剂(DES)精确控制蛋白质构象格局的能力。我们发现,二元和三元 DES 化学成分的系统性变化决定了多种构象的稳定性,即紧凑的球状折叠、中间折叠状态或未折叠链,以及控制它们的集体行为。此外,只需调整三元DES的组成,就能造访不同的构象状态,从而实现未折叠状态的再折叠,反之亦然。值得注意的是,我们发现这些中间体可以引发超分子凝胶(也称为共晶凝胶)的形成,其机械性能与蛋白质的折叠状态相关。鉴于蛋白质在水性环境中的原生折叠之外的固有脆弱性,我们的研究结果强调了DES是一种可定制的溶剂,能够通过溶剂设计按需稳定各种非原生构象。
期刊介绍:
ACS Nano, published monthly, serves as an international forum for comprehensive articles on nanoscience and nanotechnology research at the intersections of chemistry, biology, materials science, physics, and engineering. The journal fosters communication among scientists in these communities, facilitating collaboration, new research opportunities, and advancements through discoveries. ACS Nano covers synthesis, assembly, characterization, theory, and simulation of nanostructures, nanobiotechnology, nanofabrication, methods and tools for nanoscience and nanotechnology, and self- and directed-assembly. Alongside original research articles, it offers thorough reviews, perspectives on cutting-edge research, and discussions envisioning the future of nanoscience and nanotechnology.