Inducing Effect of Corylus avellana on Cytotoxic Activity in Lung and Breast Cancer Cells via Apoptosis.

IF 3.1 2区 农林科学 Q2 CHEMISTRY, APPLIED Plant Foods for Human Nutrition Pub Date : 2024-09-01 Epub Date: 2024-07-01 DOI:10.1007/s11130-024-01198-3
Ayşegül Çebi, Yalçın Tepe, İmren Alioglu, Ferda Ari
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Abstract

Turkish hazelnut (Corylus avellana L. cv Tombul) is a widely used nut in the chocolate industry and is also rich in polyphenol content, which promises anticancer effects. The anti-cancer and apoptotic effects of hazelnut leaves extracts examined on lung and breast cancer cells. Sulforhodamine B (SRB) and Adenosine 5'- triphosphate (ATP) assays were carried out for cell viability measurement. The mode of cell death was shown morphologically by the double fluorescence staining. Apoptosis was determined by performing caspase-mediated cytokeratin 18 (M30 ELISA) and western blot analysis. PARP, caspase 3, caspase 8, DR4, and GAPHD (Glyceraldehyde-3-phosphate Dehydrogenase) protein bands were visualized as markers of apoptosis. A wound healing test was employed to measure cell migration. Methanol extract of hazelnut leaf exhibited inhibition of cell growth activities in a dose-dependent manner. IC50 values were determined as 32.17 µg/ml in MCF-7, 32.16 µg/ml in MDA-MB-231, 20.40 µg/ml in A549 and 12.04 µg/ml in H1299 cells for ethanol extract while it was determined as 21.08 µg/ml in MCF-7, 40.16 µg/ml in MDA-MB-231, 22.04 µg/ml in A549 and 5.91 µg/ml in H1299 cells in methanol extract. In comparison, methanol leaf extracts were more effective in H1299 cells (IC50 value was 5.91 µg/ml).In comparison, ethanol leaf extracts were more effective in H1299 cells (IC50 value was 9.722 µg/ml). Western blot analysis demonstrated that hazelnut leaf extract treatment of cancer cells led to cell death via apoptosis and inhibited cell migration in lung and breast cancer cell lines. The cytotoxic effects of hazelnut extract on breast and lung cancer cells might be valuable and promising in elucidating cell death mechanisms for the development of new methods in cancer treatment.

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榛子通过细胞凋亡对肺癌和乳腺癌细胞毒性的诱导作用
土耳其榛子(Corylus avellana L. cv Tombul)是巧克力工业中广泛使用的一种坚果,也含有丰富的多酚,具有抗癌作用。我们研究了榛子叶提取物对肺癌和乳腺癌细胞的抗癌和凋亡作用。采用磺基多巴胺 B(SRB)和腺苷 5'- 三磷酸(ATP)测定法测量细胞活力。细胞死亡模式通过双荧光染色进行形态学分析。细胞凋亡是通过树突酶介导的细胞角蛋白 18(M30 ELISA)和蛋白印迹分析来确定的。PARP、caspase 3、caspase 8、DR4 和 GAPHD(甘油醛-3-磷酸脱氢酶)蛋白条带作为细胞凋亡的标志物。伤口愈合试验用于测量细胞迁移。榛子叶甲醇提取物对细胞生长活动的抑制作用呈剂量依赖性。乙醇提取物对 MCF-7 细胞、MDA-MB-231 细胞、A549 细胞和 H1299 细胞的 IC50 值分别为 32.17 微克/毫升、32.16 微克/毫升、20.40 微克/毫升和 12.04 微克/毫升,而甲醇提取物对 MCF-7 细胞、MDA-MB-231 细胞、A549 细胞和 H1299 细胞的 IC50 值分别为 21.08 微克/毫升、40.16 微克/毫升、22.04 微克/毫升和 5.91 微克/毫升。相比之下,甲醇叶提取物对 H1299 细胞更有效(IC50 值为 5.91 µg/ml)。Western 印迹分析表明,榛子叶提取物处理癌细胞会导致细胞凋亡,并抑制肺癌和乳腺癌细胞株的细胞迁移。榛子提取物对乳腺癌和肺癌细胞的细胞毒性作用可能对阐明细胞死亡机制、开发癌症治疗新方法具有重要价值和前景。
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来源期刊
Plant Foods for Human Nutrition
Plant Foods for Human Nutrition 工程技术-食品科技
CiteScore
6.80
自引率
7.50%
发文量
89
审稿时长
12-24 weeks
期刊介绍: Plant Foods for Human Nutrition (previously Qualitas Plantarum) is an international journal that publishes reports of original research and critical reviews concerned with the improvement and evaluation of the nutritional quality of plant foods for humans, as they are influenced by: - Biotechnology (all fields, including molecular biology and genetic engineering) - Food science and technology - Functional, nutraceutical or pharma foods - Other nutrients and non-nutrients inherent in plant foods
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