Confocal laser scanning evaluation of the influence of ledges on root canal disinfection

IF 1.3 4区医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE Australian Endodontic Journal Pub Date : 2024-06-30 DOI:10.1111/aej.12871

Elio Berutti MD, DDS, Damiano Pasqualini DDS, Nicola Scotti DDS, PhD, Narcisa Mandras PhD, Janira Roana PhD, Giorgia Carpegna DDS, PhD, Pietro Palopoli DDS, PhD, Mario Alovisi DDS, PhD

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Abstract

The aim was to analyse the influence of an apical ledge on root canal disinfection. Forty-four single-rooted teeth were micro-CT scanned and inoculated with Enterococcus faecalis. In Group S shaping was performed with ProTaper Next (PTN) up to X3 at working length (WL). In Group L an apical ledge was created with K-Files #40 and shaping completed up to PTN X3. NaOCl 5% and EDTA 10% irrigant solutions were alternated. Confocal laser scanning microscope (CLSM) and viability staining were used to analyse the proportions of dead (red) and live (green) bacteria and penetration ability inside dentinal tubules. Data were analysed with the Mann–Whitney test with Bonferroni correction (p < 0.05). In Group L the amount of red fluorescence resulted significantly lower, and penetration ability was decreased in the apical and middle portion (p < 0.05). The presence of an apical ledge may negatively influence the disinfection both in the apical and middle third.

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共焦激光扫描评估牙槽对根管消毒的影响。

目的是分析根尖壁架对根管消毒的影响。对 44 颗单根牙齿进行显微 CT 扫描并接种粪肠球菌。在 S 组中，使用 ProTaper Next (PTN) 在工作长度 (WL) X3 以下进行塑形。在 L 组中，使用 K-Files #40 制作根尖突起，并在工作长度（WL）达到 PTN X3 时完成塑形。5%的 NaOCl 和 10%的 EDTA 冲洗液交替使用。共焦激光扫描显微镜（CLSM）和活力染色用于分析牙本质小管内死亡（红色）和存活（绿色）细菌的比例以及穿透能力。数据采用 Mann-Whitney 检验和 Bonferroni 校正进行分析（p

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来源期刊

Australian Endodontic Journal DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.50

自引率

6.20%

发文量

审稿时长

>12 weeks

期刊介绍： The Australian Endodontic Journal provides a forum for communication in the different fields that encompass endodontics for all specialists and dentists with an interest in the morphology, physiology, and pathology of the human tooth, in particular the dental pulp, root and peri-radicular tissues. The Journal features regular clinical updates, research reports and case reports from authors worldwide, and also publishes meeting abstracts, society news and historical endodontic glimpses. The Australian Endodontic Journal is a publication for dentists in general and specialist practice devoted solely to endodontics. It aims to promote communication in the different fields that encompass endodontics for those dentists who have a special interest in endodontics.