Diagnosis of mucormycosis from nasal swabs using commercial PCR platforms; a feasible alternative?

IF 1.4 4区医学 Q4 IMMUNOLOGY Indian Journal of Medical Microbiology Pub Date : 2024-07-01 DOI:10.1016/j.ijmmb.2024.100661

Gagandeep Singh , Piyush Ranjan , Souradeep Chowdhury , Sunit Sikdar , Tamoghna Ghosh , Janya Sachdev , Renu Kumari Yadav , Aakashneel Bhattacharya , Mragnayani Pandey , Immaculata Xess , Ms Sonakshi Gupta , Naveet Wig

引用次数: 0

Abstract

Rhino-orbital-cerebral mucormycosis (ROCM) is linked to uncontrolled diabetes, diabetic ketoacidosis, iron overload, corticosteroid therapy, and neutropenia. This study evaluated a commercial real-time PCR system's effectiveness in detecting Mucorales from nasal swabs in 50 high-risk patients. Nasal swab PCR showed 30% positivity, compared to 8% with KOH microscopy. Despite its improved sensitivity, nasal swab PCR has limitations, highlighting the importance of established sampling methods in mucormycosis diagnosis. Participants were predominantly male (64%), with diabetes (78%) and amphotericin B use (96%). Prior COVID-19 was 42%, with 30% positive for Mucorales by PCR, compared to 8% with KOH microscopy.

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利用商用 PCR 平台从鼻拭子诊断粘孢子菌病；可行的替代方案？

鼻眶脑粘孢子菌病（ROCM）与未控制的糖尿病、糖尿病酮症酸中毒、铁超载、皮质类固醇治疗和中性粒细胞减少症有关。本研究评估了商用实时 PCR 系统从 50 名高风险患者的鼻拭子中检测粘孢子菌的有效性。鼻拭子 PCR 的阳性率为 30%，而 KOH 显微镜检查的阳性率为 8%。尽管鼻拭子 PCR 的灵敏度有所提高，但它仍有局限性，这凸显了成熟的取样方法在粘孢子菌诊断中的重要性。参与者主要为男性（64%）、糖尿病患者（78%）和两性霉素 B 使用者（96%）。COVID-19之前的阳性率为42%，其中30%通过PCR对粘孢子菌呈阳性，而通过KOH显微镜检查的阳性率为8%。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Indian Journal of Medical Microbiology IMMUNOLOGY-

CiteScore

2.20

自引率

0.00%

发文量

154

审稿时长

73 days

期刊介绍： Manuscripts of high standard in the form of original research, multicentric studies, meta analysis, are accepted. Current reports can be submitted as brief communications. Case reports must include review of current literature, clinical details, outcome and follow up. Letters to the editor must be a comment on or pertain to a manuscript already published in the IJMM or in relation to preliminary communication of a larger study. Review articles, Special Articles or Guest Editorials are accepted on invitation.