A red probe with large Stokes shift for imaging the viscosity of lipid droplets

IF 4.1 3区工程技术 Q2 CHEMISTRY, APPLIED Dyes and Pigments Pub Date : 2024-06-25 DOI:10.1016/j.dyepig.2024.112305

Zhenru Li, Qikun Zhang, Hongjin Huang, Yang Shu, Jianhua Wang

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Abstract

Lipid droplets (LDs), as intracellular lipid storage organelles, play an important role in maintaining lipid balance. The imbalance of lipid may lead to diseases, therefore, it is of great significance to monitor and evaluate the characteristics of LDs. Herein, we report the design and synthesis of a small molecular fluorescent probe MDXB (2-(2-(6-methoxy-2,3-dihydro-1H-xanthen-4-yl) vinyl) benzothiazole), which facilitates the detection of the viscosity change of intracellular LDs. MDXB follows the motion-induced change in emission (MICE) effect, wherein the –CC- bond is used as a rotation axis, and the fluorescence maybe enhanced with the increase of viscosity. MDXB exhibits a red emission at 640 nm with a large Stokes shift of ca. 180 nm, in addition to excellent photostability, which endow it with the capability of long-term imaging. The cellular imaging investigations illustrated the enhancement on the reactive oxygen species (ROS) content and the increment on LDs viscosity in the constructed cell models, e.g., cell inflammation and heavy metal ion stimulation. We thus expect MDXB to be a potential tool for the monitoring the viscosity changes of LDs and the evaluation of concurrent intracellular biological processes.

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用于成像脂滴粘度的大斯托克斯偏移红色探针

脂滴（LDs）作为细胞内储存脂质的细胞器，在维持脂质平衡方面发挥着重要作用。脂质失衡可能导致疾病，因此监测和评估脂滴的特性具有重要意义。在此，我们报告了一种小分子荧光探针 MDXB（2-(2-(6-甲氧基-2,3-二氢-1H-氧杂蒽-4-基)乙烯基)苯并噻唑）的设计与合成，它有助于检测细胞内 LDs 的粘度变化。MDXB 遵循运动诱导发射变化（MICE）效应，其中 -CC- 键被用作旋转轴，荧光可能会随着粘度的增加而增强。MDXB 在 640 纳米波长处发出红色荧光，并具有约 180 纳米波长的较大斯托克斯偏移，此外，它还具有出色的光稳定性，这赋予了它长期成像的能力。细胞成像研究表明，在构建的细胞模型（如细胞炎症和重金属离子刺激）中，活性氧（ROS）含量提高，LDs 粘度增加。因此，我们期待 MDXB 成为监测 LDs 粘度变化和评估同期细胞内生物过程的潜在工具。

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来源期刊

Dyes and Pigments 工程技术-材料科学：纺织

CiteScore

8.20

自引率

13.30%

发文量

933

审稿时长

33 days

期刊介绍： Dyes and Pigments covers the scientific and technical aspects of the chemistry and physics of dyes, pigments and their intermediates. Emphasis is placed on the properties of the colouring matters themselves rather than on their applications or the system in which they may be applied. Thus the journal accepts research and review papers on the synthesis of dyes, pigments and intermediates, their physical or chemical properties, e.g. spectroscopic, surface, solution or solid state characteristics, the physical aspects of their preparation, e.g. precipitation, nucleation and growth, crystal formation, liquid crystalline characteristics, their photochemical, ecological or biological properties and the relationship between colour and chemical constitution. However, papers are considered which deal with the more fundamental aspects of colourant application and of the interactions of colourants with substrates or media. The journal will interest a wide variety of workers in a range of disciplines whose work involves dyes, pigments and their intermediates, and provides a platform for investigators with common interests but diverse fields of activity such as cosmetics, reprographics, dye and pigment synthesis, medical research, polymers, etc.