First report of occurrence of Babesia gibsoni in captive Indian wolves

IF 1.4 Q3 PARASITOLOGY Veterinary parasitology, regional studies and reports Pub Date : 2024-06-21 DOI:10.1016/j.vprsr.2024.101071

N. Madan , P. Azhahianambi , R.P. Aravindh Babu , Neelanjana Gayen , K.G. Tirumurugaan , R. Sridhar , C. Soundararajan

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Abstract

Blood samples from fifteen captive Indian wolves (Canis lupus pallipes) maintained at Arignar Anna Zoological Park, Vandalur, Chennai were screened for the presence of Babesia spp., Ehrlichia canis and Trypnosoma evansi DNA by PCR. Out of 15 wolf samples, 3 samples were found positive for Babesia spp. The amplified 18S rRNA gene fragments from 3 wolves were sequenced and confirmed as Babesia gibsoni. A maximum likelihood tree was constructed using the three sequences along with other Babesia spp. sequences derived from GenBank adopting HKY nucleotide substitution model based on the Bayesian Information Criterion. The phylogenetic analysis confirmed that the three sequences were of Babesia gibsoni and highly divergent from Babesia canis, B. vogeli and B. vulpes. This might be a possible spill over event of B. gibsoni from community dogs through blood feeding dog ticks. This is the first report and molecular confirmation of B. gibsoni infection in captive Indian wolves.

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首次报告人工饲养的印度狼体内出现了吉布森巴贝西亚菌

通过聚合酶链式反应（PCR）对钦奈万达鲁尔 Arignar Anna 动物园饲养的 15 头印度狼（Canis lupus pallipes）的血液样本进行了巴贝西亚原虫（Babesia spp.）、犬艾氏原虫（Ehrlichia canis）和伊万斯氏原虫（Trypnosoma evansi）DNA 的筛查。对 15 个狼样本中的 3 个样本的巴贝西亚原虫检测结果呈阳性，并对 3 个狼样本的 18S rRNA 基因片段进行了测序，确认其为吉布森巴贝西亚原虫。根据贝叶斯信息标准，采用 HKY 核苷酸置换模型，利用这三个序列和 GenBank 中的其他巴贝西亚原虫序列构建了最大似然树。系统发生分析证实，这三个序列属于吉布森巴贝西亚原虫，与犬巴贝西亚原虫、沃格利巴贝西亚原虫和秃鹫巴贝西亚原虫高度不同。这可能是吉布森巴贝西亚原虫通过狗蜱吸血从社区狗身上溢出。这是首次报告和分子证实圈养的印度狼感染了吉布森氏杆菌。

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来源期刊

Veterinary parasitology, regional studies and reports Multiple-

CiteScore

2.90

自引率

7.10%

发文量

126

审稿时长

97 days

期刊介绍： Veterinary Parasitology: Regional Studies and Reports focuses on aspects of veterinary parasitology that are of regional concern, which is especially important in this era of climate change and the rapid and often unconstrained travel of people and animals. Relative to regions, this journal will accept papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites within the field of veterinary medicine. Also, case reports will be considered as they add to information related to local disease and its control; such papers must be concise and represent appropriate medical intervention. Papers on veterinary parasitology from wildlife species are acceptable, but only if they relate to the practice of veterinary medicine. Studies on vector-borne bacterial and viral agents are suitable, but only if the paper deals with vector transmission of these organisms to domesticated animals. Studies dealing with parasite control by means of natural products, both in vivo and in vitro, are more suited for one of the many journals that now specialize in papers of this type. However, due to the regional nature of much of this research, submissions may be considered based upon a case being made by the author(s) to the Editor. Circumstances relating to animal experimentation must meet the International Guiding Principles for Biomedical Research Involving Animals as issued by the Council for International Organizations of Medical Sciences (obtainable from: Executive Secretary C.I.O.M.S., c/o W.H.O., Via Appia, CH-1211 Geneva 27, Switzerland).