Engineering Bacillus licheniformis as industrial chassis for efficient bioproduction from starch

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING Bioresource Technology Pub Date : 2024-07-01 DOI:10.1016/j.biortech.2024.131061

Jiang Zhu, Min Liu, Jianling Kang, Shiyi Wang, Ziyan Zha, Yangyang Zhan, Zhi Wang, Junhui Li, Dongbo Cai, Shouwen Chen

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Abstract

Starch is an attractive feedstock in biorefinery processes, while the low natural conversion rate of most microorganisms limits its applications. Herein, starch metabolic pathway was systematically investigated using DW2 as the host organism. Initially, the effects of overexpressing amylolytic enzymes on starch hydrolysis were evaluated. Subsequently, the transmembrane transport system and intracellular degradation module were modified to accelerate the uptake of hydrolysates and their further conversion to glucose-6-phosphate. The DW2-derived strains exhibited robust growth in starch medium, and productivity of bacitracin and subtilisin were improved by 38.5% and 32.6%, with an 32.3% and 22.9% increase of starch conversion rate, respectively. Lastly, the employment of engineering strategies enabled another WX-02 to produce poly-γ-glutamic acid from starch with a 2.1-fold increase of starch conversion rate. This study not only provided excellent chassis for sustainable bioproduction from starch, but shed light on researches of substrate utilization.

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将地衣芽孢杆菌作为高效淀粉生物生产的工业底盘

淀粉是生物精炼工艺中一种极具吸引力的原料，但大多数微生物的自然转化率较低，限制了其应用。本文以 DW2 为宿主生物，对淀粉代谢途径进行了系统研究。首先，评估了过表达淀粉分解酶对淀粉水解的影响。随后，对跨膜运输系统和细胞内降解模块进行了改造，以加速水解物的吸收并进一步转化为葡萄糖-6-磷酸。DW2 衍生菌株在淀粉培养基中生长旺盛，杆菌肽和枯草菌素的生产率分别提高了 38.5% 和 32.6%，淀粉转化率分别提高了 32.3% 和 22.9%。最后，利用工程策略，另一个 WX-02 从淀粉中生产聚-γ-谷氨酸，淀粉转化率提高了 2.1 倍。这项研究不仅为淀粉的可持续生物生产提供了很好的底盘，而且为底物利用的研究提供了启示。

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来源期刊

Bioresource Technology 工程技术-能源与燃料

CiteScore

20.80

自引率

19.30%

发文量

2013

审稿时长

12 days

期刊介绍： Bioresource Technology publishes original articles, review articles, case studies, and short communications covering the fundamentals, applications, and management of bioresource technology. The journal seeks to advance and disseminate knowledge across various areas related to biomass, biological waste treatment, bioenergy, biotransformations, bioresource systems analysis, and associated conversion or production technologies. Topics include: • Biofuels: liquid and gaseous biofuels production, modeling and economics • Bioprocesses and bioproducts: biocatalysis and fermentations • Biomass and feedstocks utilization: bioconversion of agro-industrial residues • Environmental protection: biological waste treatment • Thermochemical conversion of biomass: combustion, pyrolysis, gasification, catalysis.