Bioresource Technology最新文献

2'-Fucosyllactose (2'-FL) is the most abundant human milk oligosaccharide (HMO) and has been approved to be commercially added to infant formula. Microbial synthesis from exogenous lactose via metabolic engineering is currently the major approach to production of 2'-FL. Replacement of lactose with cheaper sugars such as glucose and sucrose has been studied to reduce the production costs. Herein, Escherichia coli BL21(DE3) was engineered to produce 2'-FL by co-culture with glucose and xylose, the main components of lignocellulosic biomass. Firstly, synthetic pathway of lactose from xylose and glucose was constructed by introducing a lactose-forming enzyme, strengthening xylose uptake pathway, and weakening glucose metabolic pathway. Then, a highly-active α1,2-fucosyltransferase BKHT was introduced to produce 2'-FL and GDP-fucose supply was enhanced to increase 2'-FL production. As a result, when cocultured with glucose and xylose, the engineered strain produced 6.53 g/L and 27.53 g/L of 2'-FL by shake-flask and fed-batch cultivation, respectively.

Tannin-containing sorghum grains, suitable for acetone-butanol-ethanol (ABE) production by Clostridium acetobutylicum, have required pretreatment to eliminate tannins inhibiting the strain's amylolytic activity. This study investigates biobutanol production enhancement by immobilizing enzymes on polydopamine-functionalized polyethersulfone (PES) membranes with magnetic nanoparticles for Separated Hydrolysis and Fermentation (SHF) and Simultaneous Saccharification and Fermentation (SSF) processes. After multi-stage hot water treatment, TG3 sorghum (from the third stage) was used, where the enzyme-immobilized PES membrane produced 4.75 g/L of ABE (3.24 g/L butanol) under SSF, 0.85 g/L under SHF, and 1.1 g/L under simple fermentation. For TG6 (from the sixth stage), 3.23, 1.29, and 1.25 g/L of ABE was produced under SSF, SHF, and simple fermentation, respectively. This enhanced performance is due to the reduced enzyme inhibition. Reusability experiments showed that the membrane retained 30 % of initial activity after three cycles. These findings suggest that enzyme-immobilized membranes can intensify ABE production and enable integrated cell recovery.

In this work, the effect of the electro-assisted Fenton (EAF) process on the bacterial community of a moving bed biofilm reactor (MBBR) for olive mill wastewater (OMW) co-treatment with urban wastewater (UWW) was investigated. According to metagenomic analysis, pre-treatment by EAF, while removing total phenols (TPHs) up to 84 % ± 3 % and improving biodegradability of OMW from 0.38 to 0.62, led to the emergence of bacterial genera in the MBBR (R2) that were not detected under conditions without pre-treatment (R1). Indeed, in that condition, Candidatus Competibacter replaced Amaricoccus as dominant denitrifying bacteria. In both cases, the bacterial community composition matched with high simultaneous nitrification-denitrification efficiency (up to 98 %). Finally, Chlorobium (2.5-4.1 %), sulphate-reducing bacteria and Geobacter (up to 1.6 ± 0.4 %), anaerobic bacteria that utilise iron oxides, were observed exclusively with EAF application, suggesting potential for the development of new integrated microbial electrochemical systems.

Efficient nitrogen removal after organic capture is challenging through conventional nitrification-denitrification process. Two biofilm-based anoxic/oxic reactors, with a single intermittent zone (R1) or dual intermittent zones (R2), were compared in treating carbon-limited wastewater. Intermittent aeration integrated partial nitrification-anammox (PNA), partial denitrification-anammox (PDA), and denitrification, with anammox-related pathways contributing over 75% nitrogen removal in both reactors. As nitrogen loading rate increased from 0.14 to 0.19 kg-N m^-3 day^-1, nitrogen removal efficiency in R1 dropped from 74.3% to 46.0%, while R2 maintained 76.6% removal at low HRT of 6 h. The dual intermittent aeration strategy improved nitrogen removal capacity by enhancing PNA in the first intermittent zone and reducing effluent fluctuation in the second. Anammox bacteria (Candidatus Brocadia, relative abundance: 0.95-2.48%) were enriched across all zones, supporting efficient PNA and PDA. These findings suggested that dual intermittent aeration enhanced anammox in pre-anoxic processes for carbon limited wastewater treatment.

Biomethane production from biological methanation of CO₂ is promising both for biogas upgrading and surplus renewable energy storage. One of the questions for process upscaling is the impact of oxygen (in the biogas or in the purified CO₂-rich off-gas) on the biological process. An adapted anaerobic thermophilic consortium was submitted to increasing amounts of oxygen in batch and continuous tests at partial pressures ranging from 0 to 50 mbar. Oxygen was quickly consumed and hydrogen uptake remained similar. In the same time, methane production dropped (-4 % in continuous tests). Part of the oxygen introduced was reduced biologically by hydrogen. The amount of hydrogen diverted to oxygen reduction (up to 15 % at 50 mbar O₂) was proportional to the oxygen partial pressure. These results suggest that biological methanation systems tolerate the presence of oxygen. However, additional hydrogen should be added to maintain the conversion of CO₂ into methane.

Antimicrobial resistance (AMR) threatens public health, necessitating urgent efforts to mitigate the global impact of antibiotic resistance genes (ARGs). Anaerobic digestion (AD), known for volatile solid reduction and energy generation, also presents a feasible approach for the removal of ARGs. This review encapsulates the existing understanding of ARGs and antibiotic-resistant bacteria (ARB) during the AD process, highlighting unresolved challenges pertaining to their detection and quantification. The questions raised and discussed include: Do current ARGs detection methods meet qualitative and quantitative control requirements? How can we conduct risk assessments of ARGs? What happens to ARGs when they come into co-exposure with other emerging pollutants? How can the application of internal standards bolster the reliability of the AD resistome study? What are the potential future research directions that could enhance ARG elimination? Investigating these subjects will assist in shaping more efficient management strategies that employ AD for effective ARG control.

The temporal dynamics of bacterial and fungal communities significantly impact the manure composting process, yet viral communities are often underexplored. Bulk metagenomes, viromes, metatranscriptomes, and metabolomes were integrated to investigate dynamics of double-stranded DNA (dsDNA) virus and virus-host interactions throughout a 63-day composting process. A total of 473 viral operational taxonomic units (vOTUs), predominantly Caudoviricetes, showed distinct phase-dependent differentiation. In phase I (initial-mesophilic), viruses targeted Gammaproteobacteria and Firmicutes, utilizing restriction-modification (RM) systems. In phase II (thermophilic-maturing), viruses infected Alphaproteobacteria, Chloroflexi, and Planctomycetes, employing CRISPR-Cas systems. Lysogenic and lytic viruses exerting differential effects on bacterial pathogens across phases. Additionally, six types of auxiliary metabolic genes (AMGs) related to galactose and cysteine metabolisms were identified. The homologous lineages of AMGs with bacterial genes, along with the significant temporal correlation observed between virus-host-metabolite interactions, underscore the critical yet often overlooked role of viral communities in modulating microbial metabolisms and pathogenesis within composting ecosystems.