Bioresource Technology最新文献

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Corrigendum to “Nitrous oxide emissions treating hypersaline wastewater in suspended and attached partial nitritation – anammox reactors” [Bioresour. Technol. 424 (2025) 132266]

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-04-21 DOI: 10.1016/j.biortech.2025.132557

Lin Gao, Sheldon Tarre, Michal Green

引用次数: 0

Carbon Dioxide-Driven anaerobic digestion with Zero-Valent iron for enhanced biomethanation of food waste

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-04-21 DOI: 10.1016/j.biortech.2025.132529

Cristhian Chicaiza-Ortiz , Zhang Beihan , Jingxin Zhang , Yiliang He , Tong Yen Wah

To improve biomethanation in the anaerobic digestion of food waste (FW), carbon dioxide (CO₂) and zero-valent iron (ZVI) were applied, and the impact of varying dosages and injection times was assessed. The conditions of 1 g/L ZVI and 3-min CO₂ injection resulted in significant methane yield improvements, with FW4 reaching a peak of 624.4 mL/gVS at day 18, 44.7 % higher than the control (FW0). Hydrolysis efficiencies for proteins, polysaccharides, and lipids increased by 41 %, 65 %, and 57 %, while acidogenesis efficiencies rose by 48 %, 44 %, and 24 %, respectively. Additionally, CO₂ optimized the microbial community composition, notably increasing Methanobacterium abundance by 25.7 %. Finally, three-dimensional excitation-emission matrix (3D-EEM) fluorescence spectrograms confirmed the degradation of organic compounds, while cyclic and differential pulse voltammetry revealed increased reduction–oxidation activity. These findings underscore a promising strategy to enhance AD processes’ stability and methanogenic efficiency by combining CO₂-driven approaches with ZVI.

{"title":"Carbon Dioxide-Driven anaerobic digestion with Zero-Valent iron for enhanced biomethanation of food waste","authors":"Cristhian Chicaiza-Ortiz , Zhang Beihan , Jingxin Zhang , Yiliang He , Tong Yen Wah","doi":"10.1016/j.biortech.2025.132529","DOIUrl":"10.1016/j.biortech.2025.132529","url":null,"abstract":"<div><div>To improve biomethanation in the anaerobic digestion of food waste (FW), carbon dioxide (CO<sub>2</sub>) and zero-valent iron (ZVI) were applied, and the impact of varying dosages and injection times was assessed. The conditions of 1 g/L ZVI and 3-min CO<sub>2</sub> injection resulted in significant methane yield improvements, with FW4 reaching a peak of 624.4 mL/gVS at day 18, 44.7 % higher than the control (FW0). Hydrolysis efficiencies for proteins, polysaccharides, and lipids increased by 41 %, 65 %, and 57 %, while acidogenesis efficiencies rose by 48 %, 44 %, and 24 %, respectively. Additionally, CO<sub>2</sub> optimized the microbial community composition, notably increasing <em>Methanobacterium</em> abundance by 25.7 %. Finally, three-dimensional excitation-emission matrix (3D-EEM) fluorescence spectrograms confirmed the degradation of organic compounds, while cyclic and differential pulse voltammetry revealed increased reduction–oxidation activity. These findings underscore a promising strategy to enhance AD processes’ stability and methanogenic efficiency by combining CO<sub>2</sub>-driven approaches with ZVI.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"430 ","pages":"Article 132529"},"PeriodicalIF":9.7,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143860377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical activation of alum sludge for the adsorption of lead (Pb(II)) and arsenic (As): Mechanistic insights and machine learning (ML) analysis

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-04-20 DOI: 10.1016/j.biortech.2025.132563

Hye-Bin Kim , Muhammad Fahad Ehsan , Akram N. Alshawabkeh , Jong-Gook Kim

Alum sludge (AlS) has emerged as an effective adsorbent for anionic contaminants, with traditional activation methods like acid/base treatments and calcination employed to enhance its adsorption capacity. However, these approaches encounter significant drawbacks, including excessive waste generation, structural degradation, and limited efficacy for cationic contaminants. To overcome these challenges, this study proposes electrochemical activation as a sustainable method to enhance alum sludge adsorption performance by generating oxygen-containing functional groups (O-FGs) on its surface. In particular, cathodic activated AlS (E-AlS) leads to the formation of hydroxyl (–OH) and carboxyl (–COOH) groups, which served as key active sites for Pb(II) adsorption through complexation mechanisms. E-AlS effectively removed both Pb(II) and As within 4 h, showcasing its dual functionality for cationic and anionic contaminants. While HCl- and KOH-activated AlS also achieved 100 % Pb(II) removal, they caused substantial aluminum (Al) leaching, exceeding 1,000 mg/L, due to structural instability. In contrast, E-AlS minimized Al leaching, preserved structural integrity, and exhibited a 6.5-fold higher Pb(II) adsorption capacity than raw AlS. X-ray photoelectron spectroscopy (XPS) and machine learning (ML) validated the enhanced adsorption performance of E-AlS. These findings highlight electrochemical activation as a cost-effective and environmentally friendly remediation.

{"title":"Electrochemical activation of alum sludge for the adsorption of lead (Pb(II)) and arsenic (As): Mechanistic insights and machine learning (ML) analysis","authors":"Hye-Bin Kim , Muhammad Fahad Ehsan , Akram N. Alshawabkeh , Jong-Gook Kim","doi":"10.1016/j.biortech.2025.132563","DOIUrl":"10.1016/j.biortech.2025.132563","url":null,"abstract":"<div><div>Alum sludge (AlS) has emerged as an effective adsorbent for anionic contaminants, with traditional activation methods like acid/base treatments and calcination employed to enhance its adsorption capacity. However, these approaches encounter significant drawbacks, including excessive waste generation, structural degradation, and limited efficacy for cationic contaminants. To overcome these challenges, this study proposes electrochemical activation as a sustainable method to enhance alum sludge adsorption performance by generating oxygen-containing functional groups (O-FGs) on its surface. In particular, cathodic activated AlS (E-AlS) leads to the formation of hydroxyl (–OH) and carboxyl (–COOH) groups, which served as key active sites for Pb(II) adsorption through complexation mechanisms. E-AlS effectively removed both Pb(II) and As within 4 h, showcasing its dual functionality for cationic and anionic contaminants. While HCl- and KOH-activated AlS also achieved 100 % Pb(II) removal, they caused substantial aluminum (Al) leaching, exceeding 1,000 mg/L, due to structural instability. In contrast, E-AlS minimized Al leaching, preserved structural integrity, and exhibited a 6.5-fold higher Pb(II) adsorption capacity than raw AlS. X-ray photoelectron spectroscopy (XPS) and machine learning (ML) validated the enhanced adsorption performance of E-AlS. These findings highlight electrochemical activation as a cost-effective and environmentally friendly remediation.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"430 ","pages":"Article 132563"},"PeriodicalIF":9.7,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143860373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cultivation of high-protein Euglena gracilis for enhanced protein production under inorganic nitrogen sources: mechanisms revealed by proteomics

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-04-19 DOI: 10.1016/j.biortech.2025.132560

Qingyi He , Xueshi Zhang , Hui Xu , Xinyi Wang , Xianru Zhang , Yingshu Hao , Xinshan Song , Xin Cao

Amid global food shortage, alternative cost-effective protein sources are urgently needed for aquaculture and animal feed. Without a rigid cell wall, Euglena gracilis provides extractable, digestible proteins, and its high productivity makes it an ideal feed source. This study investigates the effects of different inorganic nitrogen sources on the biomass and biochemical composition of E. gracilis, and discusses the mechanisms of its nutrient transformation via proteomics. Results show ammonium nitrogen optimizes growth and protein accumulation by serving as an energy-efficient precursor for biomolecule synthesis compared to nitrate. Additionally, sulfate supplies sulfur for amino acid synthesis, and ammonium sulfate further enhances protein production. Under high-protein conditions, lipids and pigments increase while paramylon decreases significantly, underscoring nitrogen’s role in carbon allocation and energy metabolism. This study establishes a metabolic framework for nitrogen-sulfur coordinated regulation of protein synthesis in E. gracilis, paving the way for its industrial application as a next-generation protein resource.

{"title":"Cultivation of high-protein Euglena gracilis for enhanced protein production under inorganic nitrogen sources: mechanisms revealed by proteomics","authors":"Qingyi He , Xueshi Zhang , Hui Xu , Xinyi Wang , Xianru Zhang , Yingshu Hao , Xinshan Song , Xin Cao","doi":"10.1016/j.biortech.2025.132560","DOIUrl":"10.1016/j.biortech.2025.132560","url":null,"abstract":"<div><div>Amid global food shortage, alternative cost-effective protein sources are urgently needed for aquaculture and animal feed. Without a rigid cell wall, <em>Euglena gracilis</em> provides extractable, digestible proteins, and its high productivity makes it an ideal feed source. This study investigates the effects of different inorganic nitrogen sources on the biomass and biochemical composition of <em>E. gracilis</em>, and discusses the mechanisms of its nutrient transformation via proteomics. Results show ammonium nitrogen optimizes growth and protein accumulation by serving as an energy-efficient precursor for biomolecule synthesis compared to nitrate. Additionally, sulfate supplies sulfur for amino acid synthesis, and ammonium sulfate further enhances protein production. Under high-protein conditions, lipids and pigments increase while paramylon decreases significantly, underscoring nitrogen’s role in carbon allocation and energy metabolism. This study establishes a metabolic framework for nitrogen-sulfur coordinated regulation of protein synthesis in <em>E. gracilis</em>, paving the way for its industrial application as a next-generation protein resource<em>.</em></div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"430 ","pages":"Article 132560"},"PeriodicalIF":9.7,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carbon distribution and metabolism mechanism of a novel mixotrophic Chlorella in municipal wastewater

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-04-19 DOI: 10.1016/j.biortech.2025.132562

Xiaoyan Wang , Yu Hong , Yuewen Zhang , Dezhi Sun

Conventional wastewater treatment technologies primarily convert complex organic matter into dissolved inorganic carbon (DIC) and a more difficult gaseous state CO₂. Most microalgae species can photosynthetically assimilate above inorganic carbon, but their heterotrophic metabolic processes often dominate in glucose-mediated mixotrophy. Herein, we investigated the carbon-fixing metabolic pathways of Chlorella sp. MIHQ61 in municipal wastewater containing complex carbon sources. The total carbon removal (73.0 %) peaked on the 6th day, and DIC removal exceeded 50.0 % as the carbon migrating amount from municipal wastewater into the microalgal cells peaked. The glucose and NaHCO₃ combination promoted both autotrophic and heterotrophic metabolism. Headspace CO₂ emission, enzyme activity and central carbon metabolism results implied heterotrophic metabolism occurred more actively in the early stage and autotrophic metabolism dominated late stage. Redefined mixotrophic carbon allocation by revealing time-dependent autotrophic/heterotrophic interplay. Carbon distribution and mixotrophic mechanism provided new thinking on how to utilize microalgae and wastewater resource.

{"title":"Carbon distribution and metabolism mechanism of a novel mixotrophic Chlorella in municipal wastewater","authors":"Xiaoyan Wang , Yu Hong , Yuewen Zhang , Dezhi Sun","doi":"10.1016/j.biortech.2025.132562","DOIUrl":"10.1016/j.biortech.2025.132562","url":null,"abstract":"<div><div>Conventional wastewater treatment technologies primarily convert complex organic matter into dissolved inorganic carbon (DIC) and a more difficult gaseous state CO<sub>2</sub>. Most microalgae species can photosynthetically assimilate above inorganic carbon, but their heterotrophic metabolic processes often dominate in glucose-mediated mixotrophy. Herein, we investigated the carbon-fixing metabolic pathways of <em>Chlorella</em> sp. MIHQ61 in municipal wastewater containing complex carbon sources. The total carbon removal (73.0 %) peaked on the 6th day, and DIC removal exceeded 50.0 % as the carbon migrating amount from municipal wastewater into the microalgal cells peaked. The glucose and NaHCO<sub>3</sub> combination promoted both autotrophic and heterotrophic metabolism. Headspace CO<sub>2</sub> emission, enzyme activity and central carbon metabolism results implied heterotrophic metabolism occurred more actively in the early stage and autotrophic metabolism dominated late stage. Redefined mixotrophic carbon allocation by revealing time-dependent autotrophic/heterotrophic interplay. Carbon distribution and mixotrophic mechanism provided new thinking on how to utilize microalgae and wastewater resource.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"430 ","pages":"Article 132562"},"PeriodicalIF":9.7,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143855135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure-activity relationship of self-immobilized mycelial pellets and their functions in wastewater treatment

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-04-18 DOI: 10.1016/j.biortech.2025.132558

Li Wang , Yuqing Cao , Jiayu Wei , Shanshan Bai

Mycelial pellets (MPs) represent an emerging class of eco-friendly, self-immobilized bioactive materials characterized by high biological activity, superior porous structure, and unique biocompatibility. Based on structure–activity relationships, this paper reviews MPs' applications, mechanisms, and advantages in wastewater treatment, while updating fundamental theories on their production optimization, structure characteristics, and surface properties. Emphasis is placed on MPs' three principal functions in remediating pollution: biodegradation via high biological activity, adsorption through porous aggregated structure and superior surface features, and bio-carrier role based on the three-dimensional carbonaceous skeleton. Furthermore, the multifunctionality of MPs improves sludge settleability and dewaterability, as well as enhances aerobic granular sludge granulation and structural stability. Future research priorities include scalable low-cost production, mechanical reinforcement strategies, development of engineered strains and composites, and safe disposal of pollutant-laden MPs. This work provides valuable insights into the use of MPs in wastewater treatment and identifies critical directions for advancing MPs technology.

{"title":"Structure-activity relationship of self-immobilized mycelial pellets and their functions in wastewater treatment","authors":"Li Wang , Yuqing Cao , Jiayu Wei , Shanshan Bai","doi":"10.1016/j.biortech.2025.132558","DOIUrl":"10.1016/j.biortech.2025.132558","url":null,"abstract":"<div><div>Mycelial pellets (MPs) represent an emerging class of eco-friendly, self-immobilized bioactive materials characterized by high biological activity, superior porous structure, and unique biocompatibility. Based on structure–activity relationships, this paper reviews MPs' applications, mechanisms, and advantages in wastewater treatment, while updating fundamental theories on their production optimization, structure characteristics, and surface properties. Emphasis is placed on MPs' three principal functions in remediating pollution: biodegradation via high biological activity, adsorption through porous aggregated structure and superior surface features, and bio-carrier role based on the three-dimensional carbonaceous skeleton. Furthermore, the multifunctionality of MPs improves sludge settleability and dewaterability, as well as enhances aerobic granular sludge granulation and structural stability. Future research priorities include scalable low-cost production, mechanical reinforcement strategies, development of engineered strains and composites, and safe disposal of pollutant-laden MPs. This work provides valuable insights into the use of MPs in wastewater treatment and identifies critical directions for advancing MPs technology.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"430 ","pages":"Article 132558"},"PeriodicalIF":9.7,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143855672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microorganism interaction in microbial community, metabolic behavior and biodegradation effect on bitumen

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-04-17 DOI: 10.1016/j.biortech.2025.132555

Qiangqiang Xia , Lisha Shi , Ding Ma , Wenjing Xia , Tao Xu

To understand microorganism interaction among different microbial species in a microbial community, metabolic behavior and biodegradation effect on bitumen, Pseudomonas putida, Sphingomonas polyaromaticivorans, Bacillus cereus, Pseudomonas fluorescens, and Bacillus subtilis were compounded. The interactions among microorganisms, their metabolic products and pathways, as well as the changes in chemical component, microscopic morphology, elemental content, microstructure and micromechanical properties during bituminous biodegradation were investigated. Results show that different microorganisms achieve mutual synergy through the division of substrate and complementary metabolic functions, promoting the degradation of bitumen and generating many alcohols, phenols, carboxylic acids, fatty acids, etc. This process causes the changes in chemical components and elemental contents of bitumen, as well as a reduction in bituminous adhesion and an increase in modulus, which lowers bituminous service performance. This study provides new insights into bituminous biodegradation, and offers theoretical basis for further prolonging the service life of bituminous pavement.

{"title":"Microorganism interaction in microbial community, metabolic behavior and biodegradation effect on bitumen","authors":"Qiangqiang Xia , Lisha Shi , Ding Ma , Wenjing Xia , Tao Xu","doi":"10.1016/j.biortech.2025.132555","DOIUrl":"10.1016/j.biortech.2025.132555","url":null,"abstract":"<div><div>To understand microorganism interaction among different microbial species in a microbial community, metabolic behavior and biodegradation effect on bitumen, <em>Pseudomonas putida</em>, <em>Sphingomonas polyaromaticivorans</em>, <em>Bacillus cereus</em>, <em>Pseudomonas fluorescens</em>, and <em>Bacillus subtilis</em> were compounded. The interactions among microorganisms, their metabolic products and pathways, as well as the changes in chemical component, microscopic morphology, elemental content, microstructure and micromechanical properties during bituminous biodegradation were investigated. Results show that different microorganisms achieve mutual synergy through the division of substrate and complementary metabolic functions, promoting the degradation of bitumen and generating many alcohols, phenols, carboxylic acids, fatty acids, etc. This process causes the changes in chemical components and elemental contents of bitumen, as well as a reduction in bituminous adhesion and an increase in modulus, which lowers bituminous service performance. This study provides new insights into bituminous biodegradation, and offers theoretical basis for further prolonging the service life of bituminous pavement.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"430 ","pages":"Article 132555"},"PeriodicalIF":9.7,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143860374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dinitrogen fixation by open purple non-sulfur bacteria cultures for protein production: Diazotrophy boosts photoheterotrophic uptake rates

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-04-16 DOI: 10.1016/j.biortech.2025.132554

María del Rosario Rodero , Jean-Philippe Steyer , María Fernanda Pérez-Bernal , Willy Verstraete , Renaud Escudié , Gabriel Capson-Tojo

Purple non-sulfur bacteria (PNSB) offer a sustainable alternative to current inefficient protein production systems thanks to their high yields. This study explored the potential of specialized diazotrophic PNSB open cultures for protein production, benchmarking their performance against ammonium-grown PNSB and other diazotrophs. While diazotrophic yields (0.85–0.93 gCOD_biomass·gCOD_substrate^-1; COD being chemical oxygen demand) were slightly lower than non-diazotrophic (∼1.0), they were over double those of heterotrophic-diazotrophic rhizobacteria, with full N recovery as biomass (∼1.0 gN_biomass·gN_fixed^-1). Photoheterotrophic-diazotrophic uptake rates were the fastest ever reported for PNSB and any other diazotroph (e.g., 5.20 ± 0.83 vs. 2.64 ± 0.34 gCOD_substrate·gCOD_biomass^-1·d^-1 for PNSB on NH₄⁺). Optimal rates required high light intensities, aligning with diazotrophic energy demands. Photoheterotrophic-diazotrophic conditions were highly selective, enriching a specialized Rhodopseudomonas palustris strain. Biomass protein contents and essential amino acid metrics confirmed nutritional suitability for humans. This work lays the background for exploiting PNSB’s potential to address global protein demands through sustainable nitrogen fixation.

{"title":"Dinitrogen fixation by open purple non-sulfur bacteria cultures for protein production: Diazotrophy boosts photoheterotrophic uptake rates","authors":"María del Rosario Rodero , Jean-Philippe Steyer , María Fernanda Pérez-Bernal , Willy Verstraete , Renaud Escudié , Gabriel Capson-Tojo","doi":"10.1016/j.biortech.2025.132554","DOIUrl":"10.1016/j.biortech.2025.132554","url":null,"abstract":"<div><div>Purple non-sulfur bacteria (PNSB) offer a sustainable alternative to current inefficient protein production systems thanks to their high yields. This study explored the potential of specialized diazotrophic PNSB open cultures for protein production, benchmarking their performance against ammonium-grown PNSB and other diazotrophs. While diazotrophic yields (0.85–0.93 gCOD<sub>biomass</sub>·gCOD<sub>substrate</sub><sup>-1</sup>; COD being chemical oxygen demand) were slightly lower than non-diazotrophic (∼1.0), they were over double those of heterotrophic-diazotrophic rhizobacteria, with full N recovery as biomass (∼1.0 gN<sub>biomass</sub>·gN<sub>fixed</sub><sup>-1</sup>). Photoheterotrophic-diazotrophic uptake rates were the fastest ever reported for PNSB and any other diazotroph (e.g., 5.20 ± 0.83 vs. 2.64 ± 0.34 gCOD<sub>substrate</sub>·gCOD<sub>biomass</sub><sup>-1</sup>·d<sup>-1</sup> for PNSB on NH<sub>4</sub><sup>+</sup>). Optimal rates required high light intensities, aligning with diazotrophic energy demands. Photoheterotrophic-diazotrophic conditions were highly selective, enriching a specialized <em>Rhodopseudomonas palustris</em> strain. Biomass protein contents and essential amino acid metrics confirmed nutritional suitability for humans. This work lays the background for exploiting PNSB’s potential to address global protein demands through sustainable nitrogen fixation.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"430 ","pages":"Article 132554"},"PeriodicalIF":9.7,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143849585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ionically conductive materials for energy-efficient succinic acid recovery towards electrified circular bioeconomy

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-04-16 DOI: 10.1016/j.biortech.2025.132549

Shu-Yuan Pan , Yu-Lun Liao , Yu-I Lin , Po-Chih Tseng

In biorefineries, separation units for extracting valuable products from biomass often constitute a substantial portion of operational costs. Electrifying these separation units, such as through the use of bipolar membrane electrodeionization (BMEDI), has emerged as a key strategy to advance the circular bioeconomy. In this study, an ionically conductive material (ICM) was synthesized via sulfonation reactions under mild conditions and subsequently applied in BMEDI for the energy-efficient recovery of succinic acid. The ICM effectively alleviates the concentration polarization-induced limiting region in BMEDI, resulting in exceptional performance. The ICM-BMEDI system achieved a recovery efficiency of ∼91 %, a current efficiency of ∼90 %, a productivity of ∼0.55 kg/m²/h, and an energy consumption of ∼2.53 kWh per kg of succinic acid. Economic analysis revealed a strong benefit-cost ratio of 4.49 over a 2-year operational period. These findings highlight the potential of integrating ICM-BMEDI in biorefineries to achieve both energy-efficient and cost-effective bioproduction.

{"title":"Ionically conductive materials for energy-efficient succinic acid recovery towards electrified circular bioeconomy","authors":"Shu-Yuan Pan , Yu-Lun Liao , Yu-I Lin , Po-Chih Tseng","doi":"10.1016/j.biortech.2025.132549","DOIUrl":"10.1016/j.biortech.2025.132549","url":null,"abstract":"<div><div>In biorefineries, separation units for extracting valuable products from biomass often constitute a substantial portion of operational costs. Electrifying these separation units, such as through the use of bipolar membrane electrodeionization (BMEDI), has emerged as a key strategy to advance the circular bioeconomy. In this study, an ionically conductive material (ICM) was synthesized via sulfonation reactions under mild conditions and subsequently applied in BMEDI for the energy-efficient recovery of succinic acid. The ICM effectively alleviates the concentration polarization-induced limiting region in BMEDI, resulting in exceptional performance. The ICM-BMEDI system achieved a recovery efficiency of ∼91 %, a current efficiency of ∼90 %, a productivity of ∼0.55 kg/m<sup>2</sup>/h, and an energy consumption of ∼2.53 kWh per kg of succinic acid. Economic analysis revealed a strong benefit-cost ratio of 4.49 over a 2-year operational period. These findings highlight the potential of integrating ICM-BMEDI in biorefineries to achieve both energy-efficient and cost-effective bioproduction.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"430 ","pages":"Article 132549"},"PeriodicalIF":9.7,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143860376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of graphene oxide functionalized with nano-magnetite on swine wastewater anaerobic treatment

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2025-04-15 DOI: 10.1016/j.biortech.2025.132551

Naassom Wagner Sales Morais , Marcos Vinícius Domingos Araújo da Silva , Rennan Guthierrez Nunes do Nascimento , Valdemiro Matsumura de Sousa , Mauricio Alves da Motta Sobrinho , André Bezerra dos Santos

This study evaluated the impact of graphene oxide functionalized with nano-magnetite (GO-Fe₃O₄) on the anaerobic treatment of swine wastewater (SW). The experiment was conducted in glass reactors with 200 mL of reaction volume, operating in fed-batch mode in three treatment cycles, each with 35 days. The evaluated doses of GO-Fe₃O₄ were 3 mg L^-1 (1 mg gVSS^-1) and 150 mg L^-1 (50 mg gVSS^-1). In the third cycle, GO-Fe₃O₄ (150 mg L^-1) increased the biochemical methane potential by 17 %, the biogas production potential by 18 %, the methane production rate constant by 31 %, the maximum methane production rate by 32 %, and reduced the lag phase time by 25 %. Potential direct interspecies electron transfer partners are Midas g 156 and Clostridium sensu stricto 1 with Methanobacterium beijingense and Methanothrix soehngenii. GO-Fe₃O₄ is a powerful and unique material for improving methane and biogas production via SW anaerobic treatment.

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