Bioresource Technology最新文献

Tunable wettability of Chitosan-Biochar composites for Oil-Water separation and noble metal recovery. 壳聚糖-生物炭复合材料用于油水分离和贵金属回收的可调润湿性。

IF 9 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2026-06-01 Epub Date: 2026-03-10 DOI: 10.1016/j.biortech.2026.134395

Jie Yang, Wenya Shi, Yuyang Guo, Qianxin Long, Chao Jin, Haifeng Liu

As wastewater discharge issues in the petrochemical industry become increasingly severe, billions of tons of wastewater containing emulsified oil and trace platinum group metals (PGMs) is discharged annually, causing significant environmental pollution and resource loss. To address this challenge, biomass resources have been extensively studied for conversion into biochar via pyrolysis as an adsorbent material for wastewater treatment. Despite biochar's abundant availability and low cost, its limited specific surface area and functional groups constrain treatment efficiency. In recent years, research on biochar modification or composite materials has emerged as an effective strategy to enhance performance. Herein, we report a unified material strategy based on a chitosan-Prussian blue-biochar precursor that can be divergently processed into two distinct forms with antagonistic wettability: a hydrophilic hydrogel for PGM capture and a superhydrophobic sponge coating for oil separation. The silanized sponge composite (CPBBSM-P) exhibits excellent superhydrophobicity (water contact angle = 168.4°) and high adsorption capacities for oils and organic solvents (up to 81 g/g), enabling selective and continuous separation from water. The hydrogel composite (CPBBHM) demonstrates exceptional adsorption capacities for Pd(II) (290 mg/g) and Pt(IV) (593.67 mg/g), far surpassing many reported adsorbents. The adsorption mechanism was elucidated through systematic characterization, revealing the synergistic effect of coordination, electrostatic interaction, and reduction deposition. This work provides a novel "one-precursor-for-two-tasks" paradigm, offering a cost-effective and integrated solution for the complex challenge of petrochemical wastewater remediation.

随着石化行业废水排放问题日益严重，每年排放数十亿吨含乳化油和痕量铂族金属（PGMs）的废水，造成了严重的环境污染和资源损失。为了应对这一挑战，生物质资源已被广泛研究通过热解转化为生物炭作为废水处理的吸附材料。尽管生物炭具有丰富的可用性和低成本，但其有限的比表面积和官能团限制了处理效率。近年来，研究生物炭改性或复合材料已成为提高性能的有效策略。在此，我们报告了一种基于壳聚糖-普鲁士蓝生物炭前体的统一材料策略，该前体可以不同地加工成两种具有拮抗润湿性的不同形式：用于PGM捕获的亲水性水凝胶和用于油分离的超疏水性海绵涂层。硅化海绵复合材料（cpbbsmp）具有优异的超疏水性（水接触角 = 168.4°），对油和有机溶剂的吸附能力高（高达81 g/g），可实现与水的选择性连续分离。水凝胶复合材料（CPBBHM）对Pd(II)（290 mg/g）和Pt(IV)（593.67 mg/g）的吸附能力远远超过许多报道的吸附剂。通过系统表征阐明了吸附机理，揭示了配位、静电相互作用和还原沉积的协同作用。这项工作提供了一种新颖的“一种前体两项任务”范式，为石化废水修复的复杂挑战提供了一种具有成本效益的综合解决方案。

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引用次数: 0

Enhanced enzymatic hydrolysis of pretreated polyester textile at high solids loading through fusion with a carbohydrate binding module 通过与碳水化合物结合模块的融合，在高固体负荷下增强预处理聚酯纺织品的酶水解

IF 9 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2026-04-01 Epub Date: 2026-02-08 DOI: 10.1016/j.biortech.2026.134174

Rosie Graham , Brooke H. Wain , Robbie A. Clark , Victoria L. Bemmer , Gustavo P. Borin , Luisana Avilan , Elaine M. Rudge , Ciaran W. Lahive , Michael P. Shaver , Andrew R. Pickford

High solids loading is essential for economically and environmentally viable enzymatic recycling of poly(ethylene terephthalate) (PET), yet performance gains from enzyme–binding module fusions under industrially relevant conditions remain unclear. This study evaluated a rationally designed fusion between the thermophilic Saccharopolyspora flava cutinase and the type A carbohydrate‑binding module from Spirochaeta thermophila, selected for compatible temperature optima and substrate‑morphology preferences. The fusion enzyme retained the thermostability of its constituent domains and sustained activity at 50 °C over extended operation, while rapid loss of kinetic stability above this point accounted for poor performance at elevated temperatures. The fusion enzyme displayed increased enzyme binding capacity on both amorphous and semi‑crystalline PET, while affinity enhancement was substrate‑dependent and greatest for crystalline PET. However, enhanced adsorption on crystalline substrates did not translate into increased hydrolysis, indicating that catalytic turnover is limited by factors beyond binding, consistent with the Sabatier principle. In contrast, substantial improvements in PET depolymerisation were observed for amorphous substrates at industrially relevant (20 wt%) solids loadings under pH‑controlled reactor conditions. The largest enhancement occurred for a pre‑consumer PET textile following amorphisation and micronisation, far exceeding gains observed for amorphous films and powders. These results demonstrate that fusion enzymes have the potential to significantly enhance PET hydrolysis at high solids when substrate morphology permits productive enzyme–polymer interactions. Performance is therefore highly dependent on the specific pairing of enzyme and binding module, and on substrate properties, underscoring the need for evaluation under deployment‑relevant conditions.

高固体负载对于经济和环境可行的酶回收聚对苯二甲酸乙酯（PET）至关重要，但在工业相关条件下，酶结合模块融合的性能提高尚不清楚。本研究评估了嗜热性黄糖多孢子菌表皮酶与嗜热螺旋体a型碳水化合物结合模块之间合理设计的融合，选择了最适宜的温度和底物形态偏好。融合酶在50 °C的长时间运行中保持了其组成结构域的热稳定性和持续的活性，而在此点以上的动力学稳定性迅速丧失导致了高温下性能不佳。融合酶对非晶型和半晶型PET的结合能力均有增强，而对结晶型PET的亲和力增强与底物有关。然而，晶体底物上的增强吸附并没有转化为水解的增加，这表明催化周转受到结合以外因素的限制，与Sabatier原理一致。相比之下，在pH控制的反应器条件下，在工业相关（20 wt%）固体负载下，观察到无定形衬底的PET解聚有实质性改善。在非晶化和微粉化之后，最大的增强发生在消费前的PET纺织品上，远远超过非晶膜和粉末的增益。这些结果表明，当底物形态允许产生酶-聚合物相互作用时，融合酶具有显著增强高固体PET水解的潜力。因此，性能高度依赖于酶和结合模块的特定配对，以及底物特性，强调需要在部署相关条件下进行评估。

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引用次数: 0

Calcium-mediated mitigation of aged nanoplastic-induced stress in microalgae: Insights into photosynthesis, energy metabolism, and antioxidant defense from physiological and multi-omics analyses 钙介导的微藻衰老纳米塑料诱导的应激缓解：从生理和多组学分析中了解光合作用、能量代谢和抗氧化防御

IF 9 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2026-04-01 Epub Date: 2026-02-09 DOI: 10.1016/j.biortech.2026.134184

Yi Zhang , Xinyue Yang , Xudong Liu , Jia Feng , Shulian Xie , Junping Lv

Aged nanoplastics (ANPs) have emerged as a significant risk factor for microalgal wastewater treatment. However, Ca²⁺ appearing to have a moderating effect on this potential threat. This study investigated the response of Chlorococcum sphacosum to ANPs exposure and the protective role of Ca²⁺ through integrated physiological and multi-omics analyses. Exposure to 1 mg/L ANPs reduced the removal of NH₄⁺-N, TP, and COD by an average of 17.2%. Key physiological parameters were also suppressed, with biomass, chlorophyll a content, and photosynthetic efficiency (Fv/Fm) decreasing on average by 44.2%. Transcriptome analysis revealed that genes related to photosynthesis and energy metabolism were downregulated on average by 73.4%, highlighting the molecular basis for disrupted energy homeostasis and redox balance. Metabolomic analysis confirmed that key antioxidant metabolites, including glutathione, l-glutamic acid, linoleic acid, putrescine, and azelaic acid, decreased on average by 62.6%. These disturbances progressively caused structural deterioration, characterized by thylakoid disassembly, plasmolysis, and cell wall degradation, which impaired cellular activity and physiological function. Notably, 10 mM Ca²⁺ supplementation effectively counteracted these adverse effects. Ca²⁺ supplementation improved nutrient removal, restored physiological performance, increased antioxidant levels, mitigated transcriptional repression of photosynthetic and energy metabolism genes, and reversed the suppression of antioxidant biosynthesis. Overall, this study systematically elucidates the Ca²⁺-mediated resilience network in microalgae under ANPs exposure. These findings provide mechanistic insights and practical strategies for sustaining microalgae-based wastewater treatment in the presence of nanoplastic contamination.

老化的纳米塑料（ANPs）已成为微藻废水处理的重要危险因素。然而，Ca2+似乎对这种潜在的威胁有缓和作用。本研究通过综合生理和多组学分析，探讨了绿球对ANPs暴露的反应和Ca2+的保护作用。暴露于1 mg/LANPs中，NH4+-N、总磷和COD的去除率平均降低了17.2%。关键生理参数也受到抑制，生物量、叶绿素a含量和光合效率（Fv/Fm）平均下降44.2%。转录组分析显示，光合作用和能量代谢相关基因平均下调73.4%，强调了能量稳态和氧化还原平衡被破坏的分子基础。代谢组学分析证实，主要抗氧化代谢物，包括谷胱甘肽、l-谷氨酸、亚油酸、腐胺和壬二酸，平均下降了62.6%。这些干扰逐渐导致结构退化，以类囊体解体、质解和细胞壁降解为特征，从而损害细胞活性和生理功能。值得注意的是，10 mM Ca2+补充有效地抵消了这些不利影响。补充Ca2+可以改善营养去除，恢复生理性能，增加抗氧化水平，减轻光合和能量代谢基因的转录抑制，并逆转抗氧化生物合成的抑制。总的来说，本研究系统地阐明了ANPs暴露下微藻Ca2+介导的弹性网络。这些发现为在纳米塑料污染存在的情况下维持微藻废水处理提供了机理见解和实用策略。

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引用次数: 0

Harnessing methylotrophic methanogenesis to overcome salinity inhibition and sulfide production in anaerobic digestion 利用甲基营养化产甲烷克服厌氧消化中的盐度抑制和硫化物产生

IF 9 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2026-04-01 Epub Date: 2026-02-07 DOI: 10.1016/j.biortech.2026.134163

Korantin Pipereau , Juliana Catalina Suarez Murcia , Stéphanie Saint-Laurent , Pierre Souquet , Florian Monlau , Cecilia Sambusiti

Saline biomass represents an emerging feedstock for anaerobic digestion (AD), yet methane production is often limited by salinity and competition from sulfate-reducing microorganisms (SRM), leading to hydrogen sulfide formation. Unlike conventional methanogenesis pathway, methylotrophic pathway naturally thrives in saline environments by metabolizing methylated compounds without SRM interference nor saline inhibition. This study compared trimethylamine (TmaCl) and microcrystalline cellulose (MCC) as substrates, with or without molybdate (SRM inhibitor), at 0 and 25 g sea salt/L. Methane yields and kinetics from the methylotrophic pathway remained stable under salinity and equaled the MCC control at 0 g/L. In contrast, MCC showed a fivefold decrease in methane production and a threefold increase in lag time at high salinity. Moreover, methylotrophic conditions reduced H₂S emissions by over 30-fold. These findings demonstrate the resilience of methylotrophic methanogenesis in saline environments, offering a promising strategy for efficient valorization of marine and saline biomasses in AD.

含盐生物质是厌氧消化（AD）的新兴原料，但甲烷的产生通常受到盐度和硫酸盐还原微生物（SRM）竞争的限制，从而导致硫化氢的形成。与传统的产甲烷途径不同，甲基化营养途径在盐水环境中通过代谢甲基化化合物而不受SRM干扰或盐水抑制而自然繁荣。本研究比较了三甲胺（TmaCl）和微晶纤维素（MCC）作为底物，在0和25 g海盐/L的条件下，有或没有钼酸盐（SRM抑制剂）。甲基化营养途径的甲烷产率和动力学在盐度下保持稳定，与MCC控制在0 g/L时持平。相比之下，在高盐度条件下，MCC的甲烷产量下降了5倍，滞后时间增加了3倍。此外，甲基营养化条件将H2S排放量减少了30倍以上。这些发现证明了盐环境中甲基营养化产甲烷的恢复能力，为AD中海洋和盐水生物质的有效增值提供了一个有希望的策略。

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引用次数: 0

Novel cylindrical sulfur-based carrier (Kaldnes-S) mediating diverse S0/HS−/Sn2− pathways for enhanced autotrophic denitrification: From lab-scale biofilters to pilot-scale applications 新型圆柱形硫基载体（Kaldnes-S）介导多种S0/HS - /Sn2 -途径以增强自养反硝化：从实验室规模的生物过滤器到中试规模的应用

IF 9 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2026-04-01 Epub Date: 2026-01-27 DOI: 10.1016/j.biortech.2026.134106

Kebing Zhou , Zhiyuan Kong , Yan Zhang , Xiangchun Quan , Dongsheng Zhang , Siqi Fang

Sulfur autotrophic denitrification (SAD) is a promising biotechnology, and the development of novel sulfur-based biofilm carriers is crucial for wastewater treatment. A novel sulfur carrier (Kaldnes-S) with a cylindrical, hollow structure was developed and evaluated in laboratory-scale biofilters and pilot-scale anaerobic baffle biofilm reactors (ABBR) for treating low C/N ratio wastewater. Kaldnes-S accelerated biofilm growth, increased microbial viability by 23.6 %, enriched Thiobacillus (94.6 %), and facilitated diverse SAD pathways driven by S⁰/HS^-/S_n^2- cycling. The biofilter achieved a NO₃⁻-N removal rate of 221.7 ± 10.0 mg/(L·d) under autotrophic conditions and 717.6 ± 70.0 mg/(L·d) under mixotrophic conditions over 82 days. The pilot-scale ABBR achieved a NO₃⁻-N removal of 54.4 ± 10.6 % when treating real secondary effluent under open conditions with fluctuating dissolved oxygen (0.5–2.0 mg/L) over 257 days. This study presents a scalable, highly bioavailable sulfur carrier that improves nitrogen removal efficiency and environmental resilience, offering a sustainable solution for wastewater treatment.

硫自养反硝化（SAD）是一种很有前途的生物技术，开发新型硫基生物膜载体是污水处理的关键。研制了一种新型的圆柱形空心结构硫载体（Kaldnes-S），并在实验室规模的生物过滤器和中试规模的厌氧折流板生物膜反应器（ABBR）中对其处理低碳氮比废水进行了评价。Kaldnes-S加速了生物膜的生长，提高了23.6%的微生物活力，丰富了硫杆菌（94.6%），促进了由S0/HS-/Sn2-循环驱动的多种SAD途径。该生物滤池在自养条件下的NO3−-N去除率为221.7±10.0 mg/(L·d)，在混合营养条件下的去除率为717.6±70.0 mg/（L·d）。在开放条件下，以波动溶解氧（0.5 ~ 2.0 mg/L）处理真实的二级出水，257天，中试ABBR的NO3−-N去除率为54.4±10.6%。本研究提出了一种可扩展的、高度生物利用的硫载体，可提高氮的去除效率和环境适应性，为废水处理提供了可持续的解决方案。

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引用次数: 0

Synergistic regulation of photosynthetic efficiency at physiological and transcriptional levels in Ottelia acuminata under single and combined nitrogen and phosphorus treatments 氮磷单施和配施对水獭光合效率生理和转录水平的协同调节

IF 9 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2026-04-01 Epub Date: 2026-02-11 DOI: 10.1016/j.biortech.2026.134212

Xiong-Li Zhou , Ni-Fei Dai , Si-Qi Wang , Rui-Zhang , Zhen-Yu Lyu , Erik Jeppesen , Liu Yang , Shi-Kang Shen

Eutrophication in plateau lakes is a major global challenge driven by excessive nitrogen (N) and phosphorus (P) inputs ; however, whether N or P is the dominant limiting nutrient remains controversial. To address this issue, we investigated the responses of Ottelia acuminata to different N and P levels using integrated physiological and transcriptomic analyses. Physiological traits and chlorophyll fluorescence parameters exhibited both synergistic and antagonistic responses to N and P treatments. N primarily regulated electron transport efficiency and light energy conversion, whereas P mainly activated photoprotective mechanisms. High P alone and combined N and P treatments inhibited genes related to photosynthetic electron transport, light-harvesting complexes, and pigment metabolism, whereas high N alone upregulated genes involved in pigments, electron transport, ATPase, and the cytochrome b6/f complex. These findings enhance understanding of eutrophication adaptation and growth in O. acuminata and provide nutrient control strategies for endangered species conservation and eutrophication management in plateau lakes.

高原湖泊的富营养化是由过量的氮(N)和磷(P)输入驱动的一个重大全球挑战；然而，究竟氮还是磷是主要的限制养分仍有争议。为了解决这一问题，我们利用综合生理和转录组学分析研究了不同氮磷水平下尖锐水獭（Ottelia acuminata）的响应。生理性状和叶绿素荧光参数对氮、磷处理均表现出协同和拮抗反应。氮主要调控电子传递效率和光能转换，而磷主要激活光保护机制。高磷单独处理和氮磷联合处理抑制了光合电子传递、光收集复合物和色素代谢相关基因，而高氮单独处理上调了色素、电子传递、atp酶和细胞色素b6/f复合物相关基因。这些研究结果有助于加深对高原湖泊富营养化适应和生长的认识，并为高原湖泊濒危物种保护和富营养化管理提供营养控制策略。

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引用次数: 0

Green seaweed Ulva spp. bioprocessing: Bioactive molecules, emerging extraction technologies, and industrial applications in the circular bioeconomy 绿海藻生物加工：生物活性分子、新兴提取技术及其在循环生物经济中的工业应用

IF 9 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2026-04-01 Epub Date: 2026-01-21 DOI: 10.1016/j.biortech.2026.134066

Thomas Wichard , Paula Mapelli-Brahm , Francisco J. Barba , Rosario Domingues , Lior Guttman , Lenka Hutarova , Joana A. Loureiro , Dilek Unal , Pelin Koseoglu-Yilmaz , Gabrielle Zammit , Antonio J. Meléndez Martínez

Ulva spp., commonly known as sea lettuce, are versatile green macroalgae that offer strong potential for integration into emerging blue and circular bioeconomies. Ulva is a fast-growing and nutrient-tolerant species that produces renewable biomass rich in bioactive compounds with economic potential. This review summarizes advances in the characterization, extraction, and industrial utilization of Ulva biomass, highlighting opportunities for scalable biorefinery approaches. Key bioactive classes include sulfated polysaccharides (ulvans), proteins and peptides, polyunsaturated fatty acids, pigments, and phenolic compounds, which exhibit diverse antioxidant, antimicrobial, and anti-inflammatory properties. Recent developments in green extraction technologies, such as ultrasound-, microwave-, and enzyme-assisted methods, as well as supercritical fluids and deep eutectic solvents, have improved yields in processing Ulva biomass while reducing environmental impact. Beyond laboratory studies, industrial applications are expanding across the food, cosmetic, pharmaceutical, and biomaterial sectors, supported by growing interest in sustainable marine ingredients. However, challenges remain in biomass standardization, taxonomy, large-scale cultivation, and regulatory approval. Integrating Ulva within a circular bioeconomy requires harmonized methodologies and life-cycle assessments to ensure economic and environmental sustainability. Ulva spp. combine rapid growth, carbon capture, and a unique spectrum of bioactive compounds, making them a versatile and scalable marine feedstock for sustainable, circular biorefineries across food, health, and material applications.

Ulva spp.，俗称海莴苣，是多功能的绿色大型藻类，为融入新兴的蓝色和循环生物经济提供了强大的潜力。Ulva是一种生长迅速、耐营养的植物，可生产富含生物活性化合物的可再生生物质，具有经济潜力。本文综述了Ulva生物质的表征、提取和工业利用方面的进展，强调了可扩展生物精炼方法的机会。关键的生物活性类包括硫酸多糖（ulvans）、蛋白质和肽、多不饱和脂肪酸、色素和酚类化合物，它们具有不同的抗氧化、抗菌和抗炎特性。绿色萃取技术的最新发展，如超声波、微波和酶辅助方法，以及超临界流体和深共晶溶剂，提高了处理Ulva生物质的产量，同时减少了对环境的影响。在实验室研究之外，工业应用正在扩展到食品、化妆品、制药和生物材料领域，这得益于对可持续海洋成分日益增长的兴趣。然而，在生物质标准化、分类、大规模种植和监管审批方面仍存在挑战。将Ulva纳入循环生物经济需要统一的方法和生命周期评估，以确保经济和环境的可持续性。Ulva spp结合了快速生长，碳捕获和独特的生物活性化合物光谱，使其成为食品，健康和材料应用领域可持续循环生物炼制的多功能和可扩展的海洋原料。

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引用次数: 0

Biohydrogen evolution via ferric oxide-intercalated graphene oxide modified nickel foam biocathode in single-chamber microbial electrolysis cell 单室微生物电解池中氧化铁插层氧化石墨烯改性泡沫镍生物阴极的生物氢析出

IF 9 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2026-04-01 Epub Date: 2026-01-23 DOI: 10.1016/j.biortech.2026.134084

Hikmatullah Ahmadi , Anam Jalil , Berhanu Sugebo Helallo , Ndayisenga Fabrice , Sohail Khan , Chengyu Zhang , Zhisheng Yu

The prohibitive cost of platinum-based cathodes remains a primary constraint on scaling microbial electrolysis cells (MECs) for renewable hydrogen (H₂) production. This work addresses this barrier by developing a high-performance, cost-efficient cathode composed of a Fe₃O₄-intercalated graphene oxide nanocomposite on 3D nickel foam (Fe₃O₄-GO@NF). The electrode exhibits hydrogen evolution reaction (HER) kinetics comparable to Pt/C, evidenced by a low Tafel slope (57.91 mV·dec⁻¹) and minimal charge-transfer resistance. In single-chamber MECs fed with sewage sludge, the Fe₃O₄-GO@NF cathode delivers a sustained H₂ production rate of 49.79 mL·L⁻¹·day⁻¹ and achieves a remarkable electrical energy efficiency (η_e) of 202 % at 0.9 V. Microbial community analysis, The next-generation sequencing (NGS) of the biofilm revealed a diverse microbial consortium dominated by polysaccharide-degrading taxa (Bacteroidetes phylum) and key exoelectrogens such as Geobacter species, indicating synergistic biocatalysis. This work establishes Fe₃O₄-GO@NF as a durable, non-precious catalyst that enables efficient H₂ generation from waste, providing a viable pathway for scalable bio-electrochemical energy systems.

铂基阴极的高昂成本仍然是限制微生物电解电池（MECs）规模化生产可再生氢（H2）的主要因素。这项工作通过开发一种高性能、经济高效的阴极来解决这一障碍，该阴极由fe3o4插层氧化石墨烯纳米复合材料在3D泡沫镍上组成（Fe3O4-GO@NF）。该电极具有较低的Tafel斜率（57.91 mV·dec−1）和最小的电荷转移电阻，具有与Pt/C相当的析氢反应动力学。在以污水污泥为原料的单室mec中，Fe3O4-GO@NF阴极的产氢率持续达到49.79 mL·L−1·day−1，并在0.9 V电压下实现了202%的电能效率（ηe）。微生物群落分析：生物膜的新一代测序（NGS）显示，生物膜中存在以多糖降解类群（Bacteroidetes门）和关键外电菌（Geobacter种）为主的多种微生物群落，表明生物催化具有协同作用。这项工作建立了Fe3O4-GO@NF作为一种耐用的、非贵重的催化剂，能够从废物中高效地产生H2，为可扩展的生物电化学能源系统提供了一条可行的途径。

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引用次数: 0

Smart optimization reveals high-efficiency operational strategies for the anaerobic–oxic–anoxic process in wastewater treatment 智能优化揭示了废水处理中厌氧-氧-缺氧工艺的高效操作策略

IF 9 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2026-04-01 Epub Date: 2026-01-31 DOI: 10.1016/j.biortech.2026.134132

Yufeng Xu , Xiaonan Ding , Fang Fang , Hua Ma , Jixiang Yang

This study addressed the challenge of optimizing the continuous-flow Anaerobic–Oxic–Anoxic (AOA) process for treating low carbon-to-nitrogen ratio municipal wastewater in China. An activated sludge model was first established, which confirmed that the AOA process can be modeled, and the model results matched experimental discoveries well. Therefore, by integrating the activated sludge model with a differential evolution algorithm and supercomputing, an automated optimization framework was developed to efficiently identify optimal operational parameters. The framework successfully determined key conditions, including a hydraulic retention time of approximately 8 h (reducible to 5–6.5 h with supplementary filtration), sludge retention times of 18–22 days, and balanced reactor volume distributions favoring anoxic zones. Dynamic modeling validated the robustness of these strategies, confirming compliance with stringent effluent standards. The results demonstrate that data-driven optimization can significantly enhance process efficiency, reduce footprint, and lower operational costs. This approach can be applied to other bioprocesses, such as AAO, offering a transformative pathway for sustainable wastewater treatment.

本研究解决了中国低碳氮比城市污水的连续流厌氧-氧-缺氧（AOA）工艺优化的挑战。首先建立了活性污泥模型，证实了AOA过程可以建模，模型结果与实验结果吻合较好。因此，通过将活性污泥模型与差分进化算法和超级计算相结合，开发了一个自动化优化框架，以有效地识别最佳运行参数。该框架成功地确定了关键条件，包括水力停留时间约为8小时（通过补充过滤可减少至5-6.5小时），污泥停留时间为18-22天，以及有利于缺氧区的平衡反应器体积分布。动态建模验证了这些策略的稳健性，确认符合严格的排放标准。结果表明，数据驱动的优化可以显著提高流程效率、减少占用空间并降低运营成本。这种方法可以应用于其他生物过程，如AAO，为可持续废水处理提供了一种变革性途径。

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引用次数: 0

Metagenomic and metatranscriptomic analysis of sulfur-driven autotrophic denitrification coupled with carbon assimilation: roles of sulfur-to-nitrogen ratio and hydraulic retention time 硫驱动自养反硝化与碳同化的元基因组和元转录组分析：硫氮比和水力滞留时间的作用

IF 9 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology

Pub Date : 2026-04-01 Epub Date: 2026-02-02 DOI: 10.1016/j.biortech.2026.134153

Yuhan Xiao , Zhuowei Cheng , Jiayi Cai , Ziyi Guo , Jianmeng Chen , Yunfei Su , Feifei Cao , Dongzhi Chen

The sulfur autotrophic denitrification (SAD) process is a promising technology for nitrogen-containing wastewater treatment, with research predominantly focused on nitrate and sulfide transformations, while the potential for inorganic carbon assimilation remains underexplored. In this study, a long-term stable SAD system was maintained in an airlift bioreactor by adding an inorganic carbon source to evaluate the effects of sulfur-to-nitrogen (S/N) ratio and hydraulic retention time (HRT) on operational performance. Under optimal operating conditions (S/N = 3:2; HRT = 8 h), the system sustained high performance, achieving 99.56 ± 1.47% nitrate removal, 60.28 ± 4.19% elemental sulfur yield, and 46.48 ± 4.07% inorganic carbon assimilation efficiency. Biomass sulfur acted as a sink for extracellular free organic carbon (EFOC), thereby alleviating its accumulation-induced negative feedback on carbon assimilation. Microbial community analysis revealed a substantial enrichment of the autotrophic sulfur-oxidizing bacterium Sulfurovum, showing a marked 9.46-fold increase in relative abundance compared with the original sludge. At S/N = 3:2, metagenomic and metatranscriptomic analyses detected a 6.39-fold increase in the transcription of CBB cycle genes (rbcL/rbcS), driving a clear shift in metabolic flux toward carbon assimilation. Shortening the HRT (4 h) preferentially activated the denitrification pathway, which was evidenced by an 84.65% rise in nosZ expression (from 2,623.29 to 4,844.01 TPM), thereby bolstering N₂O reduction. Our findings offered critical insights for designing engineering solutions that enable concurrent efficient denitrification, sulfur recovery, and reduced carbon emissions.

硫自养反硝化（SAD）工艺是一种很有前途的含氮废水处理技术，研究主要集中在硝酸盐和硫化物转化上，而无机碳同化的潜力仍未得到充分开发。本研究通过添加无机碳源，在气升式生物反应器中维持一个长期稳定的SAD系统，以评估硫氮比（S/N）和水力停留时间（HRT）对运行性能的影响。在最佳运行条件下（S/N = 3:2, HRT = 8 h），系统的硝酸盐去除率为99.56±1.47%，单质硫收率为60.28±4.19%，无机碳同化效率为46.48±4.07%。生物质硫作为胞外游离有机碳（EFOC）的汇，从而减轻其积累引起的碳同化负反馈。微生物群落分析显示，自养硫氧化细菌Sulfurovum大量富集，相对丰度比原始污泥显著增加9.46倍。在S/N = 3:2时，宏基因组和亚转录组学分析发现，CBB循环基因（rbcL/rbc）的转录增加了6.39倍，导致代谢通量明显转向碳同化。缩短HRT （4 h）优先激活了反硝化途径，nosZ表达量增加84.65%（从2623.29 TPM增加到4844.01 TPM），从而促进了N2O的还原。我们的研究结果为设计工程解决方案提供了重要的见解，这些解决方案可以同时实现高效的脱硝、硫回收和减少碳排放。

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