Establishment of a promising vitiligo mouse model for pathogenesis and treatment studies.

IF 2.4 3区 医学 Q2 PATHOLOGY Diagnostic Pathology Pub Date : 2024-07-03 DOI:10.1186/s13000-024-01520-2
Ruirui Fan, Jie Gao
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Abstract

Aims: Vitiligo is a chronic dermatological condition characterized by the progressive loss of melanocytes, for which traditional therapy has shown limited efficacy. This study aimed to establish a vitiligo model with easy operability, high repeatability, and stable depigmentation to provide a foundation for studying the pathogenesis and developing novel therapies for vitiligo.

Methods: (1) Establishing vitiligo model: Firstly, deliver B16F10 cells to the back skin of C57BL/6 J via intradermal injection (day 0), and the CD4 depletion antibody was injected intraperitoneally on day 4 and 10. Secondly, the melanoma was surgically removed on day 12. Thirdly, CD8 antibody was administered intraperitoneally every fourth day till day 30. (2) Identification of vitiligo model: H&E staining, immunohistochemistry, and immunofluorescence were used to detect the melanocytes. The melanin was detected by transmission electron microscopy (TEM), Lillie ferrous sulfate staining and L-DOPA staining.

Results: (1) The back skin and hair began to appear white on day 30. Melanin loss reached peak on day 60; (2) Hematoxylin and eosin (H&E) staining, immunohistochemistry and immunofluorescence results showed melanocytes were reduced. L-DOPA staining, Lillie ferrous sulfate staining and TEM results showed that melanin decreased in the epidermis.

Conclusion: We successfully establishment a vitiligo mouse model which can be more capable to simulate the pathogenesis of human vitiligo and provide an important basis for the study of pathogenesis and therapy of vitiligo.

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建立有望用于发病机制和治疗研究的白癜风小鼠模型。
目的:白癜风是一种以黑色素细胞进行性脱失为特征的慢性皮肤病,传统疗法疗效有限。本研究旨在建立一种操作简便、重复性高、脱色斑稳定的白癜风模型,为研究白癜风的发病机制和开发新型疗法奠定基础:首先,通过皮内注射(第0天)向C57BL/6 J背部皮肤输送B16F10细胞,第4天和第10天腹腔注射CD4去势抗体。其次,在第 12 天手术切除黑色素瘤。第三,每隔第四天腹腔注射 CD8 抗体,直至第 30 天。(2) 鉴定白癜风模型:采用 H&E 染色法、免疫组化法和免疫荧光法检测黑色素细胞。结果:(1) 背部皮肤和毛发从第 30 天开始变白。结果:(1) 背部皮肤和毛发在第 30 天开始变白,黑色素脱失在第 60 天达到高峰;(2) 血红素和伊红(H&E)染色、免疫组织化学和免疫荧光结果显示黑色素细胞减少。L-DOPA染色、硫酸亚铁染色和TEM结果显示表皮中的黑色素减少:我们成功地建立了白癜风小鼠模型,该模型更能模拟人类白癜风的发病机制,为研究白癜风的发病机制和治疗提供了重要依据。
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Lydia P Schidelko, Michael Huemer, Lara M Schröder, Anna S Lueb, Josef Perner, Hannes Rakoczy
来源期刊
Diagnostic Pathology
Diagnostic Pathology 医学-病理学
CiteScore
4.60
自引率
0.00%
发文量
93
审稿时长
1 months
期刊介绍: Diagnostic Pathology is an open access, peer-reviewed, online journal that considers research in surgical and clinical pathology, immunology, and biology, with a special focus on cutting-edge approaches in diagnostic pathology and tissue-based therapy. The journal covers all aspects of surgical pathology, including classic diagnostic pathology, prognosis-related diagnosis (tumor stages, prognosis markers, such as MIB-percentage, hormone receptors, etc.), and therapy-related findings. The journal also focuses on the technological aspects of pathology, including molecular biology techniques, morphometry aspects (stereology, DNA analysis, syntactic structure analysis), communication aspects (telecommunication, virtual microscopy, virtual pathology institutions, etc.), and electronic education and quality assurance (for example interactive publication, on-line references with automated updating, etc.).
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