Lipid droplet dynamics are essential for the development of the malaria parasite Plasmodium falciparum.

IF 3.3 3区 生物学 Q3 CELL BIOLOGY Journal of cell science Pub Date : 2024-10-15 Epub Date: 2024-08-08 DOI:10.1242/jcs.262162
Jiwon Lee, Kai Matuschewski, Giel van Dooren, Alexander G Maier, Melanie Rug
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Abstract

Lipid droplets (LDs) are organelles that are central to lipid and energy homeostasis across all eukaryotes. In the malaria-causing parasite Plasmodium falciparum the roles of LDs in lipid acquisition from its host cells and their metabolism are poorly understood, despite the high demand for lipids in parasite membrane synthesis. We systematically characterised LD size, composition and dynamics across the disease-causing blood infection. Applying split fluorescence emission analysis and three-dimensional (3D) focused ion beam-scanning electron microscopy (FIB-SEM), we observed a decrease in LD size in late schizont stages. LD contraction likely signifies a switch from lipid accumulation to lipid utilisation in preparation for parasite egress from host red blood cells. We demonstrate connections between LDs and several parasite organelles, pointing to potential functional interactions. Chemical inhibition of triacylglyerol (TAG) synthesis or breakdown revealed essential LD functions for schizogony and in counteracting lipid toxicity. The dynamics of lipid synthesis, storage and utilisation in P. falciparum LDs might provide a target for new anti-malarial intervention strategies.

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脂滴动力学对恶性疟原虫的发育至关重要。
脂滴(LDs)是所有真核生物脂质和能量平衡的核心细胞器。在致疟原虫恶性疟原虫中,尽管寄生虫膜合成对脂质的需求很高,但人们对 LDs 在从宿主细胞获取脂质及其新陈代谢中的作用却知之甚少。我们系统地描述了致病血液感染过程中 LD 的大小、组成和动态。应用分裂荧光发射分析和三维聚焦离子束扫描电子显微镜,我们观察到裂殖晚期的 LD 体积减小。LD收缩可能意味着寄生虫从脂质积累转向脂质利用,为寄生虫从宿主红细胞中排出做好准备。我们证明了 LD 与几个寄生虫细胞器之间的联系,指出了潜在的功能性相互作用。对三酰基甘油(TAG)合成或分解的化学抑制揭示了 LD 在分裂和抵消脂质毒性方面的重要功能。恶性疟原虫LD中脂质合成、储存和利用的动态变化可能为新的抗疟干预策略提供了目标。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of cell science
Journal of cell science 生物-细胞生物学
CiteScore
7.30
自引率
2.50%
发文量
393
审稿时长
1.4 months
期刊介绍: Journal of Cell Science publishes cutting-edge science, encompassing all aspects of cell biology.
期刊最新文献
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