Histopathological growth pattern and vessel co-option in intrahepatic cholangiocarcinoma.

IF 1.2 4区医学 Q3 PATHOLOGY Medical Molecular Morphology Pub Date : 2024-09-01 Epub Date: 2024-07-03 DOI:10.1007/s00795-024-00392-1

Zihan Li, Hiep Nguyen Canh, Kenta Takahashi, Dong Le Thanh, Quynh Nguyen Thi, Rui Yang, Kaori Yoshimura, Yasunori Sato, Khuyen Nguyen Thi, Hiroki Nakata, Hiroko Ikeda, Kazuto Kozaka, Satoshi Kobayashi, Shintaro Yagi, Kenichi Harada

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Abstract

Intrahepatic cholangiocarcinoma (iCCA) exhibits different blood imaging features and prognosis depending on histology. To clarity histopathological growth patterns (HGPs) and vascularization processes of iCCA, we collected 145 surgical specimens and histologically classified them into large bile duct (LBD) (20 cases), small bile duct (SBD) (54), cholangiolocarcinoma (CLC) (35), combined SBD-CLC (cSBD-CLC) (26), and ductal plate malformation (DPM) (10) (sub)types. According to the invasive pattern at the interface between tumor and adjacent background liver, HGPs were classified into desmoplastic, pushing, and replacing HGPs. Desmoplastic HGP predominated in LBD type (55.5%), while replacing HGP was common in CLC (82.9%) and cSBD-CLC (84.6%) subtypes. Desmoplastic HGP reflected angiogenesis, while replacing HGP showed vessel co-option in addition to angiogenesis. By evaluating microvessel density (MVD) using vascular markers, ELTD1 identified vessel co-option and angiogenesis, and ELTD1-positive MVD at invasive margin in replacing HGP was significantly higher than those in desmoplastic and pushing HGPs. REDD1, an angiogenesis-related marker, demonstrated preferably higher MVD in the tumor center than in other areas. iCCA (sub)types and HGPs were closely related to vessel co-option and immune-related factors (lymphatic vessels, lymphocytes, and neutrophils). In conclusion, HGPs and vascular mechanisms characterize iCCA (sub)types and vessel co-option linked to the immune microenvironment.

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肝内胆管癌的组织病理学生长模式和血管并存。

肝内胆管癌（iCCA）根据组织学表现出不同的血液影像学特征和预后。为了明确 iCCA 的组织病理学生长模式（HGPs）和血管化过程，我们收集了 145 例手术标本，并在组织学上将其分为大胆管（LBD）（20 例）、小胆管（SBD）（54 例）、胆管癌（CLC）（35 例）、联合 SBD-CLC （cSBD-CLC）（26 例）和导管板畸形（DPM）（10 例）（亚）型。根据肿瘤与邻近背景肝脏交界处的浸润模式，HGP 被分为去瘤型 HGP、推移型 HGP 和替代型 HGP。去瘤型 HGP 主要见于 LBD 型（55.5%），而替换型 HGP 常见于 CLC（82.9%）和 cSBD-CLC （84.6%）亚型。去鳞屑型 HGP 反映了血管生成，而替代型 HGP 则显示了血管生成之外的血管增生。通过使用血管标记物评估微血管密度（MVD），ELTD1确定了血管共生和血管生成，替代型HGP侵袭边缘的ELTD1阳性MVD明显高于去瘤型和推移型HGP。iCCA（亚）型和 HGP 与血管增生和免疫相关因素（淋巴管、淋巴细胞和中性粒细胞）密切相关。总之，HGPs和血管机制是iCCA（亚）类型和血管共用与免疫微环境相关的特征。

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来源期刊

Medical Molecular Morphology 医学-病理学

CiteScore

2.90

自引率

5.60%

发文量

审稿时长

>12 weeks

期刊介绍： Medical Molecular Morphology is an international forum for researchers in both basic and clinical medicine to present and discuss new research on the structural mechanisms and the processes of health and disease at the molecular level. The structures of molecules, organelles, cells, tissues, and organs determine their normal function. Disease is thus best understood in terms of structural changes in these different levels of biological organization, especially in molecules and molecular interactions as well as the cellular localization of chemical components. Medical Molecular Morphology welcomes articles on basic or clinical research in the fields of cell biology, molecular biology, and medical, veterinary, and dental sciences using techniques for structural research such as electron microscopy, confocal laser scanning microscopy, enzyme histochemistry, immunohistochemistry, radioautography, X-ray microanalysis, and in situ hybridization. Manuscripts submitted for publication must contain a statement to the effect that all human studies have been reviewed by the appropriate ethics committee and have therefore been performed in accordance with the ethical standards laid down in an appropriate version of the 1964 Declaration of Helsinki. It should also be stated clearly in the text that all persons gave their informed consent prior to their inclusion in the study. Details that might disclose the identity of the subjects under study should be omitted.