Ahmet Aktar, Mehmed Berk Toker, Davut Koca, Umit Can Uzun, Selim Alcay
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引用次数: 0
Abstract
Objectives: This study aimed to examine the effects of supplementation of vitamin D to the egg-yolk extender on characteristics of frozen-thawed ram semen.
Methods: Semen samples obtained from adult rams were pooled and divided into five equal volumes. It was reconstituted with extenders containing different concentrations of vitamin D: 0 (control), 12.5 (VITD 12.5), 25 (VITD 25), 50 (VITD 50), and 100 ng/mL (VITD 100), and then they were frozen. Sperm motility parameters, plasma membrane functional integrity, acrosomal integrity, DNA fragmentation, and mitochondrial membrane potential of the groups were evaluated after sperm thawing.
Results: Total motility and progressive motility were higher in VITD 50 than in all other groups (p < 0.05). Higher sperm straightness, linearity, and wooble were higher in VITD 50 than in the control group (p < 0.05). A similar pattern of VITD 50 was observed for plasma membrane integrity and mitochondrial membrane potential (p > 0.05).
Conclusions: In the study, it was observed that adding vitamin D to the extender had a beneficial effect on ram spermatological parameters. In addition, it was concluded that the use of the 50 ng/mL vitamin D in the extender provided more effective protection than the other doses.
研究目的本研究旨在探讨在蛋黄扩展剂中补充维生素 D 对冷冻解冻公羊精液特征的影响：方法：将成年公羊的精液样本集中起来，分成五等分。用含有不同浓度维生素 D 的扩展剂：0（对照组）、12.5（VITD 12.5）、25（VITD 25）、50（VITD 50）和 100 纳克/毫升（VITD 100）重组精液，然后冷冻。解冻后对各组精子的运动参数、质膜功能完整性、顶体完整性、DNA碎片和线粒体膜电位进行评估：结果：VITD 50 的总运动能力和渐进运动能力均高于其他各组（P 0.05）：研究观察到，在扩增剂中添加维生素 D 对公羊精子学参数有好处。此外，研究还得出结论，与其他剂量相比，在扩增剂中添加 50 纳克/毫升维生素 D 可提供更有效的保护。
期刊介绍：
Veterinary Medicine and Science is the peer-reviewed journal for rapid dissemination of research in all areas of veterinary medicine and science. The journal aims to serve the research community by providing a vehicle for authors wishing to publish interesting and high quality work in both fundamental and clinical veterinary medicine and science.
Veterinary Medicine and Science publishes original research articles, systematic reviews, meta-analyses, and research methods papers, along with invited editorials and commentaries. Original research papers must report well-conducted research with conclusions supported by the data presented in the paper.
We aim to be a truly global forum for high-quality research in veterinary medicine and science, and believe that the best research should be published and made widely accessible as quickly as possible. Veterinary Medicine and Science publishes papers submitted directly to the journal and those referred from a select group of prestigious journals published by Wiley-Blackwell.
Veterinary Medicine and Science is a Wiley Open Access journal, one of a new series of peer-reviewed titles publishing quality research with speed and efficiency. For further information visit the Wiley Open Access website.
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