首页 > 最新文献

Veterinary Medicine and Science最新文献

英文 中文
Detection and characterization of multidrug resistant Escherichia coli carrying virulence gene isolated from broilers in Bangladesh. 从孟加拉国肉鸡中分离出的携带毒力基因的耐多药大肠杆菌的检测和特征描述。
IF 1.7 3区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2024-11-01 DOI: 10.1002/vms3.70032
Md Sirazul Islam,Chandan Nath,F M Yasir Hasib,Tahia Ahmed Logno,Md Helal Uddin,Mohammad Mahmudul Hassan,Sharmin Chowdhury
BACKGROUNDThe emergence and dissemination of multidrug resistant (MDR) bacteria pose a severe threat to public health by limiting clinical treatment and prophylactic options.OBJECTIVESThis study investigates the prevalence of Escherichia coli in broilers, their phenotypic antimicrobial resistance (AMR) profiles and the presence of virulence-associated genes (VAGs) and antimicrobial resistance genes (ARGs) using polymerase chain reaction (PCR).MATERIALS AND METHODSA total of 216 pooled cloacal samples were collected from 1080 broilers across six districts of Bangladesh. Each pooled sample comprised randomly selected cloacal swabs from five birds per farm. E. coli isolates were identified using standard bacteriological approach, followed by biochemical assays and PCR. Antimicrobial susceptibility was assessed using the Kirby-Bauer disc diffusion method, and the presence of ARGs and VAGs was determined via PCR. Five selected isolates were partially sequenced for five VAGs using Sanger sequencing.RESULTSA total of 177 E. coli isolates (81.94%, 95% confidence interval: 76.24%-86.53%) were identified. The isolates showed the highest resistance to ampicillin (93.79%), followed by tetracycline (91.53%), erythromycin (89.27%) and ciprofloxacin (87%). Conversely, ceftriaxone (80.79%) showed highest susceptibility, followed by gentamicin (37.29%) and neomycin (31.07%). All isolates were MDR, with a multiple antibiotic resistance indexes were <0.3. A significant percentage (16.38%) of E. coli isolates were MDR to five antimicrobial classes and harboured blaTEM, sul1, ere (A), tetA, tetB and tetC genes. The highest prevalent ARGs were blaTEM (88.14%) followed by ere (A) (83.62%) and sul 1 (72.32%). The prevalence of VAGs was astA (56.50%), iucD (31.07%), iss (21.47%), irp2 (15.82%) and cva/cvi (3.39%), respectively.CONCLUSIONSThis study highlights the presence of ARGs contributing to the development of MDR in E. coli carrying VAGs in broilers. Effective monitoring and surveillance of antimicrobial usage in poultry production systems are urgently required to prevent emergence and dissemination of AMR.
背景耐多药(MDR)细菌的出现和传播限制了临床治疗和预防选择,对公共卫生构成严重威胁。本研究使用聚合酶链式反应(PCR)方法调查了肉鸡中大肠埃希氏菌的流行率、表型抗菌药耐药性(AMR)概况以及毒力相关基因(VAG)和抗菌药耐药性基因(ARG)的存在情况。每个集合样本由每个农场随机抽取的 5 只鸡的泄殖腔拭子组成。采用标准细菌学方法对分离出的大肠杆菌进行鉴定,然后进行生化检测和 PCR 检测。抗菌药敏感性采用柯比-鲍尔盘扩散法进行评估,ARGs 和 VAGs 的存在通过 PCR 确定。结果共鉴定出 177 个大肠杆菌分离物(81.94%,95% 置信区间:76.24%-86.53%)。分离物对氨苄西林的耐药性最高(93.79%),其次是四环素(91.53%)、红霉素(89.27%)和环丙沙星(87%)。相反,头孢曲松(80.79%)的敏感性最高,其次是庆大霉素(37.29%)和新霉素(31.07%)。所有分离菌株都具有多重耐药性,多重抗生素耐药性指数均小于 0.3。相当大比例(16.38%)的大肠埃希氏菌分离物对五类抗菌药具有耐药性,并携带 blaTEM、sul1、ere (A)、tetA、tetB 和 tetC 基因。流行率最高的 ARGs 是 blaTEM(88.14%),其次是 ere (A)(83.62%)和 sul 1(72.32%)。VAGs的流行率分别为astA(56.50%)、iucD(31.07%)、iss(21.47%)、irp2(15.82%)和cva/cvi(3.39%)。迫切需要对家禽生产系统中抗菌药物的使用情况进行有效的监测和监控,以防止 AMR 的出现和传播。
{"title":"Detection and characterization of multidrug resistant Escherichia coli carrying virulence gene isolated from broilers in Bangladesh.","authors":"Md Sirazul Islam,Chandan Nath,F M Yasir Hasib,Tahia Ahmed Logno,Md Helal Uddin,Mohammad Mahmudul Hassan,Sharmin Chowdhury","doi":"10.1002/vms3.70032","DOIUrl":"https://doi.org/10.1002/vms3.70032","url":null,"abstract":"BACKGROUNDThe emergence and dissemination of multidrug resistant (MDR) bacteria pose a severe threat to public health by limiting clinical treatment and prophylactic options.OBJECTIVESThis study investigates the prevalence of Escherichia coli in broilers, their phenotypic antimicrobial resistance (AMR) profiles and the presence of virulence-associated genes (VAGs) and antimicrobial resistance genes (ARGs) using polymerase chain reaction (PCR).MATERIALS AND METHODSA total of 216 pooled cloacal samples were collected from 1080 broilers across six districts of Bangladesh. Each pooled sample comprised randomly selected cloacal swabs from five birds per farm. E. coli isolates were identified using standard bacteriological approach, followed by biochemical assays and PCR. Antimicrobial susceptibility was assessed using the Kirby-Bauer disc diffusion method, and the presence of ARGs and VAGs was determined via PCR. Five selected isolates were partially sequenced for five VAGs using Sanger sequencing.RESULTSA total of 177 E. coli isolates (81.94%, 95% confidence interval: 76.24%-86.53%) were identified. The isolates showed the highest resistance to ampicillin (93.79%), followed by tetracycline (91.53%), erythromycin (89.27%) and ciprofloxacin (87%). Conversely, ceftriaxone (80.79%) showed highest susceptibility, followed by gentamicin (37.29%) and neomycin (31.07%). All isolates were MDR, with a multiple antibiotic resistance indexes were <0.3. A significant percentage (16.38%) of E. coli isolates were MDR to five antimicrobial classes and harboured blaTEM, sul1, ere (A), tetA, tetB and tetC genes. The highest prevalent ARGs were blaTEM (88.14%) followed by ere (A) (83.62%) and sul 1 (72.32%). The prevalence of VAGs was astA (56.50%), iucD (31.07%), iss (21.47%), irp2 (15.82%) and cva/cvi (3.39%), respectively.CONCLUSIONSThis study highlights the presence of ARGs contributing to the development of MDR in E. coli carrying VAGs in broilers. Effective monitoring and surveillance of antimicrobial usage in poultry production systems are urgently required to prevent emergence and dissemination of AMR.","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142258950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Seroprevalence of Avian Influenza in Guinea Fowls in Some Districts in the Upper East Region of Ghana. 加纳上东部地区部分地区家禽的禽流感血清流行率。
IF 1.8 3区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2024-11-01 DOI: 10.1002/vms3.70106
Albert Agyapong Tweneboah, Sherry Ama Mawuko Johnson, Patrick Mensah Amponsah, Derrick Adu Asare, Benjamin Obukowho Emikpe

The guinea fowl (Numida meleagris) holds significant agricultural importance in Ghana, particularly in the Northern, Upper East and Upper West Regions. Despite their economic and cultural significance, guinea fowls face a potential threat from avian influenza, a global concern for its adverse impact on poultry populations. This study assessed the seroprevalence of the virus in mature guinea fowls in the Upper East Region. A cross-sectional survey was conducted in three districts within the Upper East Region from April to June 2023. Blood samples were collected from 397 guinea fowls that are over 4 weeks old, and seroprevalence was determined using ID Screen Influenza A Antibody Competition Enzyme-linked immunosorbent assay (ELISA). The study analysed demographic factors such as sex, age and source of birds, employing statistical methods to establish associations. Among the sampled guinea fowls, 24.7% tested positive for avian influenza antibodies, whereas 75.3% were seronegative. Age did not show statistically significant associations with seroprevalence, but intriguing patterns were observed. Adult guinea fowls exhibited higher seroprevalence (23.7%) compared to growers (1.0%). The source of birds showed no significant association, but birds from slaughter points demonstrated higher seroprevalence (11.6%) compared to households (5.0%) and live bird markets (8.1%). In conclusion, the study underscores the importance of monitoring avian influenza in guinea fowls to implement effective control measures. The presence of antibodies suggests guinea fowls may contribute to virus transmission in the Upper East Region. The study recommends ongoing nationwide surveillance to assess the true prevalence of avian influenza in guinea fowls across Ghana.

珍珠鸡(Numida meleagris)在加纳具有重要的农业意义,尤其是在北部、上东部和上西部地区。尽管珍珠鸡具有重要的经济和文化意义,但它们也面临着禽流感的潜在威胁。本研究评估了上东部地区成熟珍珠鸡的病毒血清流行率。这项横断面调查于 2023 年 4 月至 6 月在上东部地区的三个区进行。从397只4周龄以上的珍珠鸡身上采集了血样,并使用ID筛查甲型流感抗体竞争酶联免疫吸附试验(ELISA)测定了血清流行率。研究分析了鸟类的性别、年龄和来源等人口统计学因素,并采用统计方法建立关联。在采样的珍珠鸡中,24.7%的检测结果呈禽流感抗体阳性,75.3%呈血清阴性。年龄与血清阳性率没有明显的统计学关联,但观察到了一些有趣的模式。成年珍珠鸡的血清流行率(23.7%)高于生长鸡(1.0%)。禽鸟的来源没有明显关联,但屠宰点禽鸟的血清流行率(11.6%)高于家庭(5.0%)和活禽市场(8.1%)。总之,这项研究强调了监测珍珠鸡禽流感以实施有效控制措施的重要性。抗体的存在表明,珍珠鸡可能会导致病毒在上东部地区的传播。研究建议继续在全国范围内开展监测,以评估加纳各地珍珠鸡中禽流感的真实流行情况。
{"title":"Seroprevalence of Avian Influenza in Guinea Fowls in Some Districts in the Upper East Region of Ghana.","authors":"Albert Agyapong Tweneboah, Sherry Ama Mawuko Johnson, Patrick Mensah Amponsah, Derrick Adu Asare, Benjamin Obukowho Emikpe","doi":"10.1002/vms3.70106","DOIUrl":"10.1002/vms3.70106","url":null,"abstract":"<p><p>The guinea fowl (Numida meleagris) holds significant agricultural importance in Ghana, particularly in the Northern, Upper East and Upper West Regions. Despite their economic and cultural significance, guinea fowls face a potential threat from avian influenza, a global concern for its adverse impact on poultry populations. This study assessed the seroprevalence of the virus in mature guinea fowls in the Upper East Region. A cross-sectional survey was conducted in three districts within the Upper East Region from April to June 2023. Blood samples were collected from 397 guinea fowls that are over 4 weeks old, and seroprevalence was determined using ID Screen Influenza A Antibody Competition Enzyme-linked immunosorbent assay (ELISA). The study analysed demographic factors such as sex, age and source of birds, employing statistical methods to establish associations. Among the sampled guinea fowls, 24.7% tested positive for avian influenza antibodies, whereas 75.3% were seronegative. Age did not show statistically significant associations with seroprevalence, but intriguing patterns were observed. Adult guinea fowls exhibited higher seroprevalence (23.7%) compared to growers (1.0%). The source of birds showed no significant association, but birds from slaughter points demonstrated higher seroprevalence (11.6%) compared to households (5.0%) and live bird markets (8.1%). In conclusion, the study underscores the importance of monitoring avian influenza in guinea fowls to implement effective control measures. The presence of antibodies suggests guinea fowls may contribute to virus transmission in the Upper East Region. The study recommends ongoing nationwide surveillance to assess the true prevalence of avian influenza in guinea fowls across Ghana.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11522813/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142547797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
First Molecular Identification and Whole Genome Sequencing of Listeria monocytogenes Isolated From an African Lion. 首次对从非洲狮身上分离出的李斯特菌进行分子鉴定和全基因组测序。
IF 1.8 3区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2024-11-01 DOI: 10.1002/vms3.70110
Ping Xu, Xinyong Qi, Xiaoxu Wang, Feng Xu, Hongjin Zhao, Liping Shen, Yujie Zhang, Shixin Huang, Jian Wang

Listeria monocytogenes (LM) is a zoonotic pathogen that causes sporadic infectious listeriosis, which is a foodborne disease associated with consumption of contaminated food or feed. The internal organs of an African lion from a zoo in Shanghai were analysed to determine the cause of death. LM infection was suspected on the basis of the clinical symptoms and pathological changes and confirmed by polymerase chain reaction, whole genome sequencing and phylogenetic analysis. This is the first report of LM infection of an African lion in China.

单核细胞增生李斯特菌(LM)是一种人畜共患病原体,可引起散发性传染性李斯特菌病,这是一种与食用受污染食物或饲料有关的食源性疾病。上海一家动物园对一只非洲狮的内脏进行了分析,以确定其死因。根据临床症状和病理变化怀疑感染了 LM,并通过聚合酶链式反应、全基因组测序和系统发育分析进行了确认。这是中国首次报道非洲狮感染LM。
{"title":"First Molecular Identification and Whole Genome Sequencing of Listeria monocytogenes Isolated From an African Lion.","authors":"Ping Xu, Xinyong Qi, Xiaoxu Wang, Feng Xu, Hongjin Zhao, Liping Shen, Yujie Zhang, Shixin Huang, Jian Wang","doi":"10.1002/vms3.70110","DOIUrl":"10.1002/vms3.70110","url":null,"abstract":"<p><p>Listeria monocytogenes (LM) is a zoonotic pathogen that causes sporadic infectious listeriosis, which is a foodborne disease associated with consumption of contaminated food or feed. The internal organs of an African lion from a zoo in Shanghai were analysed to determine the cause of death. LM infection was suspected on the basis of the clinical symptoms and pathological changes and confirmed by polymerase chain reaction, whole genome sequencing and phylogenetic analysis. This is the first report of LM infection of an African lion in China.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11533306/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142569726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Socio-economic evaluation of human-dog coexistence: A 40,000 years history. 人狗共存的社会经济评估:四万年的历史
IF 1.8 3区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2024-11-01 DOI: 10.1002/vms3.70012
Mohammad Fallahi, Ali Akbar Masoudi, Rasoul Vaez Torshizi, Ali Maghsoudi

The human-animal relationship and its benefits have been a popular issue in recent decades. This study focuses on the dog as a paradigmatic example of this complex bond. The relationship between human and dog evolved over thousands of years through the domestication process. During this period, climatic, cultural, geographical and social changes have had a direct impact on this bond. Dogs have a remarkable capacity to play a variety of roles that mirror the evolution of human societies. Subsequently, questions arise regarding the important and symbolic roles that canines played in ancient cultures, religions and mythological traditions. Specifically, how have these roles changed through history? This research aimed to explore the socio-economic aspects of the human-dog relationship, spanning from ancient civilizations to modern societies. Through a systematic search methodology, this investigation seeks to provide insights into the dynamics of the complex human-dog relationship, including historical backgrounds, socio-cultural dynamics, economic implications and associated challenges. Additionally, the present study addresses the environmental concerns that emerge alongside rising stray dog populations. This research emphasizes the importance of strategies to balance the benefits of this coexistence that promote human and animal welfare.

近几十年来,人与动物的关系及其益处一直是一个热门话题。本研究将狗作为这一复杂关系的典型例子。人与狗之间的关系经过数千年的驯化过程演变而来。在此期间,气候、文化、地理和社会的变化对这种关系产生了直接影响。狗具有扮演各种角色的非凡能力,这些角色反映了人类社会的演变。因此,人们对犬在古代文化、宗教和神话传统中扮演的重要和象征性角色产生了疑问。具体来说,这些角色在历史上发生了怎样的变化?本研究旨在探索从古代文明到现代社会中人犬关系的社会经济方面。通过系统的搜索方法,本研究试图深入了解复杂的人狗关系的动态,包括历史背景、社会文化动态、经济影响和相关挑战。此外,本研究还探讨了随着流浪狗数量增加而出现的环境问题。这项研究强调了制定战略的重要性,以平衡这种促进人类和动物福利的共存关系所带来的益处。
{"title":"Socio-economic evaluation of human-dog coexistence: A 40,000 years history.","authors":"Mohammad Fallahi, Ali Akbar Masoudi, Rasoul Vaez Torshizi, Ali Maghsoudi","doi":"10.1002/vms3.70012","DOIUrl":"10.1002/vms3.70012","url":null,"abstract":"<p><p>The human-animal relationship and its benefits have been a popular issue in recent decades. This study focuses on the dog as a paradigmatic example of this complex bond. The relationship between human and dog evolved over thousands of years through the domestication process. During this period, climatic, cultural, geographical and social changes have had a direct impact on this bond. Dogs have a remarkable capacity to play a variety of roles that mirror the evolution of human societies. Subsequently, questions arise regarding the important and symbolic roles that canines played in ancient cultures, religions and mythological traditions. Specifically, how have these roles changed through history? This research aimed to explore the socio-economic aspects of the human-dog relationship, spanning from ancient civilizations to modern societies. Through a systematic search methodology, this investigation seeks to provide insights into the dynamics of the complex human-dog relationship, including historical backgrounds, socio-cultural dynamics, economic implications and associated challenges. Additionally, the present study addresses the environmental concerns that emerge alongside rising stray dog populations. This research emphasizes the importance of strategies to balance the benefits of this coexistence that promote human and animal welfare.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11464907/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142393684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of the Diversities in the Inflammatory Responses in Cats With Bacterial and Viral Infections. 评估猫细菌和病毒感染后炎症反应的多样性
IF 1.8 3区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2024-11-01 DOI: 10.1002/vms3.70098
Songul Erhan, Bengu Bilgic, Ezgi Ergen, Mert Erek, Elif Ergul Ekiz, Mukaddes Ozcan, Mehmet Erman Or, Banu Dokuzeylul, Erdal Matur

Background: Understanding the nature of inflammatory responses in cats with bacterial and viral infections is essential for accurately managing the infection. This study aimed to investigate the diversities of inflammatory responses between bacterial and viral infections in cats to figure out their role in the pathophysiology of these infections.

Methods: Seventy-five owned cats were included in the study. The evaluations were performed based on three groups: healthy control, bacterial infection group (those with bronchopneumonia and gastrointestinal tract and urinary tract infections) and viral infection group (21 with feline coronavirus [FCoV], 3 with feline leukaemia virus [FeLV] and 1 with feline calicivirus), each containing 25 individuals. Total and differential leukocyte counts, C-reactive protein (CRP), transforming growth factor beta (TGF-β), interleukin-6 (IL-6), tumour necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β) and interleukin-10 (IL-10) concentrations were assessed in the blood samples collected from sick and healthy animals.

Results: No statistically significant difference was noted in serum TNF-α, IL-1β and IL-10 concentrations of the infected cats (p = 0.996, p = 0.160 and p = 0.930, respectively). Serum TGF-β concentration in the viral infection group was reduced compared to the healthy control (p = 0.001). In contrast, WBC count and IL-6 and CRP concentrations were increased in the cats with bronchopneumonia, gastrointestinal tract infections and urinary tract infections compared to the healthy control and viral infection groups (p = 0.001, p = 0.001 and p = 0.001, respectively).

Conclusion: This study revealed significant differences between bacterial and viral infections regarding the fashion of inflammatory responses in cats, and the relevant data will undoubtedly contribute to the management and control of feline infectious diseases, rendering the development of novel therapeutic strategies.

背景:了解猫细菌和病毒感染时炎症反应的性质对于准确控制感染至关重要。本研究旨在调查猫细菌和病毒感染时炎症反应的多样性,以了解它们在这些感染的病理生理学中的作用:研究纳入了 75 只猫咪。评估分为三组:健康对照组、细菌感染组(支气管肺炎、胃肠道和泌尿道感染)和病毒感染组(21 只感染猫冠状病毒[FCoV],3 只感染猫白血病病毒[FeLV],1 只感染猫卡里西病毒),每组 25 只。从患病动物和健康动物采集的血液样本中评估了白细胞总数和差值、C 反应蛋白 (CRP)、转化生长因子 beta (TGF-β)、白细胞介素-6 (IL-6)、肿瘤坏死因子-α (TNF-α)、白细胞介素-1 beta (IL-1β) 和白细胞介素-10 (IL-10) 的浓度:感染猫血清中 TNF-α、IL-1β 和 IL-10 的浓度在统计学上没有明显差异(分别为 p = 0.996、p = 0.160 和 p = 0.930)。与健康对照组相比,病毒感染组的血清 TGF-β 浓度有所降低(p = 0.001)。相反,与健康对照组和病毒感染组相比,支气管肺炎、胃肠道感染和尿路感染猫的白细胞计数、IL-6 和 CRP 浓度均有所增加(分别为 p = 0.001、p = 0.001 和 p = 0.001):这项研究揭示了细菌感染和病毒感染在猫的炎症反应方式上的显著差异,相关数据无疑将有助于猫科传染病的管理和控制,并促进新型治疗策略的开发。
{"title":"Evaluation of the Diversities in the Inflammatory Responses in Cats With Bacterial and Viral Infections.","authors":"Songul Erhan, Bengu Bilgic, Ezgi Ergen, Mert Erek, Elif Ergul Ekiz, Mukaddes Ozcan, Mehmet Erman Or, Banu Dokuzeylul, Erdal Matur","doi":"10.1002/vms3.70098","DOIUrl":"10.1002/vms3.70098","url":null,"abstract":"<p><strong>Background: </strong>Understanding the nature of inflammatory responses in cats with bacterial and viral infections is essential for accurately managing the infection. This study aimed to investigate the diversities of inflammatory responses between bacterial and viral infections in cats to figure out their role in the pathophysiology of these infections.</p><p><strong>Methods: </strong>Seventy-five owned cats were included in the study. The evaluations were performed based on three groups: healthy control, bacterial infection group (those with bronchopneumonia and gastrointestinal tract and urinary tract infections) and viral infection group (21 with feline coronavirus [FCoV], 3 with feline leukaemia virus [FeLV] and 1 with feline calicivirus), each containing 25 individuals. Total and differential leukocyte counts, C-reactive protein (CRP), transforming growth factor beta (TGF-β), interleukin-6 (IL-6), tumour necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β) and interleukin-10 (IL-10) concentrations were assessed in the blood samples collected from sick and healthy animals.</p><p><strong>Results: </strong>No statistically significant difference was noted in serum TNF-α, IL-1β and IL-10 concentrations of the infected cats (p = 0.996, p = 0.160 and p = 0.930, respectively). Serum TGF-β concentration in the viral infection group was reduced compared to the healthy control (p = 0.001). In contrast, WBC count and IL-6 and CRP concentrations were increased in the cats with bronchopneumonia, gastrointestinal tract infections and urinary tract infections compared to the healthy control and viral infection groups (p = 0.001, p = 0.001 and p = 0.001, respectively).</p><p><strong>Conclusion: </strong>This study revealed significant differences between bacterial and viral infections regarding the fashion of inflammatory responses in cats, and the relevant data will undoubtedly contribute to the management and control of feline infectious diseases, rendering the development of novel therapeutic strategies.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11522849/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142547795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluations of Morphometric Traits and Body Conformation Indices of Horse Ecotypes Reared in the Highlands of Bale Eco-Region, Ethiopia. 埃塞俄比亚巴莱生态区高原饲养的马生态型的形态特征和体形指数评估
IF 1.8 3区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2024-11-01 DOI: 10.1002/vms3.70114
Mesay Guyo, Melaku Tareke, Andualem Tonamo, Diriba Bediye, Girma Defar

Background: Morphometric traits discrepancies are associated with production, reproduction, adaptation and behaviours in horses. It also determines the design of harnessing implements, physical injury level and working performance of the native horses. Thus, the study was conducted to evaluate morphometric traits and body conformation indices of horse ecotypes reared across four districts of Bale Zone, Ethiopia.

Methods: Data of croup height, croup length, hip height, mid-back height, wither height, hip width, thoracic depth, chest width, body length, sternum height, thorax perimeter and cannon perimeter were measured in 500 horses (294 male and 206 female). Using those traits, 17 body conformation indices were calculated to define the horses for multi-faceted performances.

Results: The study revealed that croup height, hip height, wither height, hip width, croup length, body length, thorax perimeter and sternum height were significantly (p < 0.0001) different among the studied districts. The district effect was also significant at p < 0.05 for mid-back height, thoracic depth, chest width and cannon perimeter. The sexual dimorphism effect was statistically significant for all measured traits; however, females were inferior to males except for cannon perimeter. Body conformation indices of pelvic index, body index, transversal pelvic index and weight were highly significant (p < 0.0001) among the studied districts. The sex dimorphism effect was significant (p < 0.001) for dactyl-costal index, pelvic index, longitudinal pelvic index, transversal pelvic index, weight, tare index 1 (trot or gallop), tare index 2 (walk) and riding comfort degree, while non-significant (p > 0.05) for the remaining indices. The studied horses were classified as mediline, elipometric, light, small and suitable riding ecotypes with a medium-conforming body structure and good capacity for carrying loads on carts and backs for functional effectiveness. The correlations of body conformation indices ranged from very high to low, with positive and negative associations for functional capabilities.

Conclusion: Therefore, selection for improvement needs to rank morphometric traits and body conformation indices in correlation to developing racing and modern sport horses in the highlands of the Bale eco-regions. Further, molecular studies will be essential to determine whether the predominated phenotypic multiplicity is genetic or environmentally progressed.

背景:形态特征差异与马的生产、繁殖、适应和行为有关。它还决定了本地马的挽具设计、身体损伤程度和工作表现。因此,本研究对埃塞俄比亚巴勒区四个地区饲养的马匹生态型的形态特征和体形指数进行了评估:方法:测量了 500 匹马(294 匹雄马和 206 匹雌马)的颈高、颈长、臀高、中背高、腰高、臀宽、胸深、胸宽、体长、胸骨高、胸廓周长和加农炮周长。利用这些特征,计算出了 17 个体态指数,以确定马匹的多方面表现:研究结果表明,在所研究的地区之间,马鞍高、臀高、腰高、臀宽、马鞍长、体长、胸围和胸骨高有显著差异(p < 0.0001)。在中背高、胸廓深度、胸宽和胸廓周长方面,地区效应也有显著差异(p < 0.05)。性二型效应对所有测量性状都有显著的统计学意义;然而,除炮周长外,雌性均劣于雄性。在所研究的地区中,骨盆指数、体指数、骨盆横向指数和体重等体型指数具有高度显著性(P < 0.0001)。鬐甲-肋骨指数、骨盆指数、纵向骨盆指数、横向骨盆指数、体重、皮重指数 1(小跑或快跑)、皮重指数 2(步行)和骑乘舒适度的性别二态效应显著(p < 0.001),而其余指数则不显著(p > 0.05)。所研究的马匹被归类为中等体型、椭圆体型、轻体型、小体型和适合骑乘的生态型马匹,它们的身体结构适中,具有良好的推车和背负能力,功能有效。体型指数的相关性从很高到很低不等,与功能能力的相关性有正有负:因此,为了在巴勒生态区高原地区发展赛马和现代运动马,在改良选育时需要对形态特征和体型指数进行排序。此外,还必须开展分子研究,以确定主要的表型多样性是遗传因素还是环境因素。
{"title":"Evaluations of Morphometric Traits and Body Conformation Indices of Horse Ecotypes Reared in the Highlands of Bale Eco-Region, Ethiopia.","authors":"Mesay Guyo, Melaku Tareke, Andualem Tonamo, Diriba Bediye, Girma Defar","doi":"10.1002/vms3.70114","DOIUrl":"10.1002/vms3.70114","url":null,"abstract":"<p><strong>Background: </strong>Morphometric traits discrepancies are associated with production, reproduction, adaptation and behaviours in horses. It also determines the design of harnessing implements, physical injury level and working performance of the native horses. Thus, the study was conducted to evaluate morphometric traits and body conformation indices of horse ecotypes reared across four districts of Bale Zone, Ethiopia.</p><p><strong>Methods: </strong>Data of croup height, croup length, hip height, mid-back height, wither height, hip width, thoracic depth, chest width, body length, sternum height, thorax perimeter and cannon perimeter were measured in 500 horses (294 male and 206 female). Using those traits, 17 body conformation indices were calculated to define the horses for multi-faceted performances.</p><p><strong>Results: </strong>The study revealed that croup height, hip height, wither height, hip width, croup length, body length, thorax perimeter and sternum height were significantly (p < 0.0001) different among the studied districts. The district effect was also significant at p < 0.05 for mid-back height, thoracic depth, chest width and cannon perimeter. The sexual dimorphism effect was statistically significant for all measured traits; however, females were inferior to males except for cannon perimeter. Body conformation indices of pelvic index, body index, transversal pelvic index and weight were highly significant (p < 0.0001) among the studied districts. The sex dimorphism effect was significant (p < 0.001) for dactyl-costal index, pelvic index, longitudinal pelvic index, transversal pelvic index, weight, tare index 1 (trot or gallop), tare index 2 (walk) and riding comfort degree, while non-significant (p > 0.05) for the remaining indices. The studied horses were classified as mediline, elipometric, light, small and suitable riding ecotypes with a medium-conforming body structure and good capacity for carrying loads on carts and backs for functional effectiveness. The correlations of body conformation indices ranged from very high to low, with positive and negative associations for functional capabilities.</p><p><strong>Conclusion: </strong>Therefore, selection for improvement needs to rank morphometric traits and body conformation indices in correlation to developing racing and modern sport horses in the highlands of the Bale eco-regions. Further, molecular studies will be essential to determine whether the predominated phenotypic multiplicity is genetic or environmentally progressed.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11538250/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142584411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exploration of Some Nutrient Absorption-Related Genes and Intestinal Histology in Japanese Quails (Coturnix japonica) Throughout Their Productive Lifespan. 探究日本鹌鹑(Coturnix japonica)整个生育期与营养吸收相关的基因和肠道组织学。
IF 1.8 3区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2024-11-01 DOI: 10.1002/vms3.70056
Hamid Reza Rafieian-Naeini, Mahdi Zhandi, Maryam Taghipour-Shahbandi, Amir Reza Sabzian, Ali Reza Yousefi, Mahboubeh Khajehnabi, Woo Kyun Kim

Background: Quail is an efficient poultry species known for many years, and its productivity is highly affected by gastrointestinal function, health, digestion and commensal bacteria. Specific histological parameters and nutrient absorption-related genes could be used to study intestinal functionality and efficiency.

Objectives: This study aimed to evaluate the mRNA abundance of nutrient absorption-related genes and intestinal histology changes in laying Japanese quail during productive lifespan.

Methods: A total of 135 quails from a commercial flock at different egg production periods (early production [EP, 6 weeks of age], pick production [PP, 21 weeks of age] and post pick production [PPP, 40 weeks of age]) were randomly selected (n = 45/group) and kept for 6 weeks. Production performance was evaluated during last 2 weeks of the experimental period, and tissue samples were taken from the duodenum, jejunum and ileum for histological analysis and mRNA abundance purposes at the end of the experiment. The mRNA abundance of the CALB1, SGLT1, SGLT5, EAAT3 and NaPi-IIb genes were compared among groups.

Results: Production period did not affect the eggshell thickness, goblet cell number and mRNA abundance of CALB1, EAAT3 and NaPi-IIb (p > 0.05). The PPP quails had the highest egg weight and FCR compared to EP and PP quails (p < 0.001). Egg production and egg mass of the EP and PP quails were higher than those of PPP quails (p < 0.001). Moreover, EP quails had the highest villus height (VH)/crypt depth (CD) ratio in the duodenum and jejunum and the lowest VH:CD in the ileum compared with the PPP quails (p < 0.01). The lowest SGLT1 mRNA abundance was noted in the PP quails, whereas EP quails had the highest SGLT5 mRNA abundance (p < 0.05).

Conclusions: In conclusion, although histology parameter changes were in-line with the lowest performance observed in the PPP quails, the current results failed to show a relationship between mRNA abundance changes and production performance attributes at different productive lifespans.

背景:鹌鹑是一种高效家禽,其生产率受肠道功能、健康、消化和共生菌的影响很大。特定的组织学参数和营养吸收相关基因可用于研究肠道功能和效率:本研究旨在评估产蛋日本鹌鹑在产蛋期营养吸收相关基因的 mRNA 丰度和肠道组织学变化:随机选取135只处于不同产蛋期(早期产蛋期[EP,6周龄]、采摘期[PP,21周龄]和采摘后产蛋期[PPP,40周龄])的商品鹌鹑(n = 45/组),饲养6周。在实验的最后两周评估生产性能,并在实验结束时从十二指肠、空肠和回肠采集组织样本进行组织学分析和 mRNA 丰度分析。比较各组之间 CALB1、SGLT1、SGLT5、EAAT3 和 NaPi-IIb 基因的 mRNA 丰度:结果:生产期对蛋壳厚度、腺细胞数量以及 CALB1、EAAT3 和 NaPi-IIb 的 mRNA 丰度均无影响(p > 0.05)。与 EP 和 PP 型鹌鹑相比,PPP 型鹌鹑的蛋重和 FCR 最高(p 结论:PPP 型鹌鹑的蛋重和 FCR 均低于 EP 型鹌鹑:总之,虽然组织学参数变化与 PPP 鹌鹑的最低生产性能一致,但目前的结果未能显示 mRNA 丰度变化与不同生产寿命的生产性能属性之间的关系。
{"title":"Exploration of Some Nutrient Absorption-Related Genes and Intestinal Histology in Japanese Quails (Coturnix japonica) Throughout Their Productive Lifespan.","authors":"Hamid Reza Rafieian-Naeini, Mahdi Zhandi, Maryam Taghipour-Shahbandi, Amir Reza Sabzian, Ali Reza Yousefi, Mahboubeh Khajehnabi, Woo Kyun Kim","doi":"10.1002/vms3.70056","DOIUrl":"10.1002/vms3.70056","url":null,"abstract":"<p><strong>Background: </strong>Quail is an efficient poultry species known for many years, and its productivity is highly affected by gastrointestinal function, health, digestion and commensal bacteria. Specific histological parameters and nutrient absorption-related genes could be used to study intestinal functionality and efficiency.</p><p><strong>Objectives: </strong>This study aimed to evaluate the mRNA abundance of nutrient absorption-related genes and intestinal histology changes in laying Japanese quail during productive lifespan.</p><p><strong>Methods: </strong>A total of 135 quails from a commercial flock at different egg production periods (early production [EP, 6 weeks of age], pick production [PP, 21 weeks of age] and post pick production [PPP, 40 weeks of age]) were randomly selected (n = 45/group) and kept for 6 weeks. Production performance was evaluated during last 2 weeks of the experimental period, and tissue samples were taken from the duodenum, jejunum and ileum for histological analysis and mRNA abundance purposes at the end of the experiment. The mRNA abundance of the CALB1, SGLT1, SGLT5, EAAT3 and NaPi-IIb genes were compared among groups.</p><p><strong>Results: </strong>Production period did not affect the eggshell thickness, goblet cell number and mRNA abundance of CALB1, EAAT3 and NaPi-IIb (p > 0.05). The PPP quails had the highest egg weight and FCR compared to EP and PP quails (p < 0.001). Egg production and egg mass of the EP and PP quails were higher than those of PPP quails (p < 0.001). Moreover, EP quails had the highest villus height (VH)/crypt depth (CD) ratio in the duodenum and jejunum and the lowest VH:CD in the ileum compared with the PPP quails (p < 0.01). The lowest SGLT1 mRNA abundance was noted in the PP quails, whereas EP quails had the highest SGLT5 mRNA abundance (p < 0.05).</p><p><strong>Conclusions: </strong>In conclusion, although histology parameter changes were in-line with the lowest performance observed in the PPP quails, the current results failed to show a relationship between mRNA abundance changes and production performance attributes at different productive lifespans.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11519991/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142523243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Determination of Species-Specific Differences in Intracranial Volume of Tuj Sheep and Hair Goats Using Stereology and Computed Tomography Methods. 利用立体学和计算机断层扫描方法确定土家族绵羊和毛山羊颅内容积的物种特异性差异。
IF 1.8 3区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2024-11-01 DOI: 10.1002/vms3.70111
Ali Koçyiğit, Betül Kanik, İsmail Demircioğlu, Yasin Demiraslan

The intracranial cavity contains vitally important organs. The brain, cerebellum, meninges and the vessels that supply these organs are located in the intracranial cavity. Therefore, it is important to learn about the intracranial cavity and to study it. However, there is limited information about the intracranial cavity in the veterinary field. The aim of this study was to determine the differences between the intracranial cavities of different species of animals by using stereology and tomography methods, volume calculations and morphometric measurements. In addition, the compatibility of the methods used with each other was investigated. In the study, six male adult goats and six male adult sheep were used. In this study, the intracranial cavities of sheep and goats were calculated by using Cavalieri's principle and 3D modelling using tomography sections. Morphometric measurements were taken over the intracranial space, and index calculations were made. In 3D models using computed tomography, the intracranial volume was 153.31 ± 24.06 cm3 in goats and 128.07 ± 7.93 cm3 in sheep. In the calculation using Cavalieri's principle, it was determined to be 152.73 ± 22.73 cm3 in goats and 126.15 ± 8.38 cm3 in sheep. As a result of the study, the MWCC (maximum width of the cranial cavity) parameter was found to be statistically significant between species (p < 0.05). The two methods used in Bland-Altman analysis were found to be within the limits of agreement, and the methods can be alternative to each other.

颅内腔包含重要的器官。大脑、小脑、脑膜和供应这些器官的血管都位于颅内腔。因此,了解和研究颅内腔非常重要。然而,兽医领域关于颅内腔的信息非常有限。本研究旨在通过立体学和断层扫描方法、体积计算和形态测量,确定不同物种动物颅内腔的差异。此外,还研究了所用方法之间的兼容性。这项研究使用了六只雄性成年山羊和六只雄性成年绵羊。在这项研究中,绵羊和山羊的颅内腔是通过卡瓦列里原理和使用断层扫描切片进行三维建模计算得出的。对颅内空间进行了形态测量,并进行了指数计算。在使用计算机断层扫描的三维模型中,山羊的颅内容积为 153.31 ± 24.06 立方厘米,绵羊的颅内容积为 128.07 ± 7.93 立方厘米。根据卡瓦列里原理计算,山羊的颅内容积为 152.73 ± 22.73 立方厘米,绵羊的颅内容积为 126.15 ± 8.38 立方厘米。研究结果表明,MWCC(颅腔最大宽度)参数在不同物种之间具有统计学意义(P < 0.05)。发现布兰-阿尔特曼分析中使用的两种方法在一致的范围内,这两种方法可以相互替代。
{"title":"Determination of Species-Specific Differences in Intracranial Volume of Tuj Sheep and Hair Goats Using Stereology and Computed Tomography Methods.","authors":"Ali Koçyiğit, Betül Kanik, İsmail Demircioğlu, Yasin Demiraslan","doi":"10.1002/vms3.70111","DOIUrl":"10.1002/vms3.70111","url":null,"abstract":"<p><p>The intracranial cavity contains vitally important organs. The brain, cerebellum, meninges and the vessels that supply these organs are located in the intracranial cavity. Therefore, it is important to learn about the intracranial cavity and to study it. However, there is limited information about the intracranial cavity in the veterinary field. The aim of this study was to determine the differences between the intracranial cavities of different species of animals by using stereology and tomography methods, volume calculations and morphometric measurements. In addition, the compatibility of the methods used with each other was investigated. In the study, six male adult goats and six male adult sheep were used. In this study, the intracranial cavities of sheep and goats were calculated by using Cavalieri's principle and 3D modelling using tomography sections. Morphometric measurements were taken over the intracranial space, and index calculations were made. In 3D models using computed tomography, the intracranial volume was 153.31 ± 24.06 cm<sup>3</sup> in goats and 128.07 ± 7.93 cm<sup>3</sup> in sheep. In the calculation using Cavalieri's principle, it was determined to be 152.73 ± 22.73 cm<sup>3</sup> in goats and 126.15 ± 8.38 cm<sup>3</sup> in sheep. As a result of the study, the MWCC (maximum width of the cranial cavity) parameter was found to be statistically significant between species (p < 0.05). The two methods used in Bland-Altman analysis were found to be within the limits of agreement, and the methods can be alternative to each other.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11533207/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142569721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development and Application of an Indirect Enzyme-Linked Immunosorbent Assay Based on a Recombinant Matrix Protein for the Serological Study of Porcine Deltacoronavirus in Mexican Pigs. 基于重组基质蛋白的间接酶联免疫吸附测定法在墨西哥猪猪瘟病毒血清学研究中的开发与应用
IF 1.8 3区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2024-11-01 DOI: 10.1002/vms3.70108
Francisco Jesus Castañeda Montes, José Luis Cerriteño Sánchez, Julieta Sandra Cuevas-Romero, María Azucena Castañeda Montes, Susana Mendoza Elvira

Porcine deltacoronavirus (PDCoV) is an infectious disease that causes diarrhoea in pigs of different ages; however, piglets are more susceptible. PDCoV was first reported in 2012 in China and Hong Kong. Later, it was first reported in the USA in 2014 and in Mexico in 2019. Several studies have shown that M protein is highly conserved and, therefore, suitable for diagnostic systems. In this study, for the first time, an indirect enzyme-linked immunosorbent assay (iELISA) based on a recombinant M protein (rM-PDCoV) was developed to evaluate the seroprevalence of PDCoV in four states in Mexico. High sensitivity (83%) and specificity (100%) were observed for the iELISA. The kappa index calculated a nearly perfect agreement (0.8831) compared to the Western blot (gold standard test), suggesting acceptable statistical value support. In this study, 50.38% of the serum samples from backyard pigs were PDCoV-positive. The serological comparison showed that PDCoV/PEDV coinfections occurred in 31.98% of the analysed sera. These results can enrich our understanding of how this virus spreads and enable the evaluation of PDCoV infections. Moreover, it highlights the importance of continually investigating the seroprevalence of PDCoV in Mexico because there is also no information about the current prevalence of the disease.

猪瘟病毒(PDCoV)是一种传染病,可导致不同年龄的猪腹泻,但仔猪更易感染。PDCoV 于 2012 年首次在中国和香港报告。随后,2014 年和 2019 年分别在美国和墨西哥首次报道。多项研究表明,M 蛋白具有高度保守性,因此适用于诊断系统。本研究首次开发了一种基于重组 M 蛋白(rM-PDCoV)的间接酶联免疫吸附试验(iELISA),用于评估墨西哥四个州的 PDCoV 血清流行率。iELISA 的灵敏度(83%)和特异性(100%)都很高。卡帕指数(kappa index)与西方印迹(金标检测)相比,计算出的结果几乎完全一致(0.8831),这表明统计值支持是可以接受的。在这项研究中,50.38% 的散养猪血清样本呈 PDCoV 阳性。血清学比较显示,31.98% 的分析血清出现 PDCoV/PEDV 共感染。这些结果丰富了我们对该病毒传播途径的了解,并有助于对 PDCoV 感染进行评估。此外,它还强调了继续调查墨西哥 PDCoV 血清流行率的重要性,因为目前还没有关于该疾病流行率的信息。
{"title":"Development and Application of an Indirect Enzyme-Linked Immunosorbent Assay Based on a Recombinant Matrix Protein for the Serological Study of Porcine Deltacoronavirus in Mexican Pigs.","authors":"Francisco Jesus Castañeda Montes, José Luis Cerriteño Sánchez, Julieta Sandra Cuevas-Romero, María Azucena Castañeda Montes, Susana Mendoza Elvira","doi":"10.1002/vms3.70108","DOIUrl":"10.1002/vms3.70108","url":null,"abstract":"<p><p>Porcine deltacoronavirus (PDCoV) is an infectious disease that causes diarrhoea in pigs of different ages; however, piglets are more susceptible. PDCoV was first reported in 2012 in China and Hong Kong. Later, it was first reported in the USA in 2014 and in Mexico in 2019. Several studies have shown that M protein is highly conserved and, therefore, suitable for diagnostic systems. In this study, for the first time, an indirect enzyme-linked immunosorbent assay (iELISA) based on a recombinant M protein (rM-PDCoV) was developed to evaluate the seroprevalence of PDCoV in four states in Mexico. High sensitivity (83%) and specificity (100%) were observed for the iELISA. The kappa index calculated a nearly perfect agreement (0.8831) compared to the Western blot (gold standard test), suggesting acceptable statistical value support. In this study, 50.38% of the serum samples from backyard pigs were PDCoV-positive. The serological comparison showed that PDCoV/PEDV coinfections occurred in 31.98% of the analysed sera. These results can enrich our understanding of how this virus spreads and enable the evaluation of PDCoV infections. Moreover, it highlights the importance of continually investigating the seroprevalence of PDCoV in Mexico because there is also no information about the current prevalence of the disease.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11533212/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142569723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of the Effect of Adding Different Levels of Zinc Chloride, Curcumin, Zinc Oxide Nanoparticles (Zano-NPs), Curcumin Loaded on Zano-NPs on Post-Thawing Quality of Ram Semen. 比较添加不同水平的氯化锌、姜黄素、氧化锌纳米颗粒(Zano-NPs)和添加姜黄素的 Zano-NPs 对公羊精液解冻后质量的影响。
IF 1.8 3区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2024-11-01 DOI: 10.1002/vms3.70091
Fatemeh Omidi, Hadi Hajarian, Hamed Karamishabankareh, Leila Soltani, Mojtaba Dashtizad

Objective: This study looked at how different concentrations of curcumin (Curc), zinc chloride (ZnCl2), zinc oxide nanoparticles (ZnO-NPs) and Curc loaded on ZnO-NPs (Curc-co-ZnO-NPs) in cryopreservation dilution affected the quality of ram sperm after thawing.

Methods: ZnO-NPs were synthesised using Berberis vulgaris leaf aqueous extract. Then, Curc was loaded on the ZnO-NPs that had been synthesised. We used analytical methods to look at the composition, morphology and size of green synthesised ZnO-NPs and Curc-co-ZnO-NPs, including UV-Vis, zeta potential, EDX, DLS, FE-SEM and FT-IR. Using a Tris-base extender containing various concentrations of Curc, ZnCl2, ZnO-NPs and Curc-co-ZnO-NPs (0, 1, 10 and 100 µg/mL), semen samples from four rams were combined. Sperm motility, viability, DNA and plasma membrane integrity, total abnormalities and malondialdehyde (MDA) generation were all evaluated in treatment groups after thawing.

Results: The results showed that adding 1 µg/mL of ZnO-NPs and Curc-co-ZnO-NPs significantly reduced the level of MDA and total abnormalities (p < 0.05). Additionally, following the freeze-thawing procedure, the presence of 1 µg/mL of Curc-co-ZnO-NPs in the diluent of ram sperm significantly increased the percentage of sperm viability and motility in comparison to the control and other treatment groups (p < 0.05). Furthermore, as compared to the control group and other treatments, treatments containing 1 µg/mL of Curc-co-ZnO-NPs significantly improved membrane and DNA integrity (p < 0.05).

Conclusions: It appears that following freeze-thawing, the Curc-co-ZnO-NPs (1 µg/mL) enhanced sperm parameters.

研究目的本研究探讨了不同浓度的姜黄素(Curc)、氯化锌(ZnCl2)、氧化锌纳米颗粒(ZnO-NPs)和姜黄素负载于氧化锌纳米颗粒(Curc-co-ZnO-NPs)在冷冻稀释液中对解冻后公羊精子质量的影响:方法:使用小檗叶水提取物合成 ZnO-NPs。然后,在合成的 ZnO-NPs 上载入 Curc。我们使用分析方法来观察绿色合成 ZnO-NPs 和 Curc-co-ZnO-NPs 的成分、形态和大小,包括紫外可见光、ZETA 电位、EDX、DLS、FE-SEM 和 FT-IR。使用含有不同浓度 Curc、ZnCl2、ZnO-NPs 和 Curc-co-ZnO-NPs(0、1、10 和 100 µg/mL)的三碱扩展剂,将四种公羊的精液样本混合在一起。解冻后对各处理组的精子活力、存活率、DNA 和质膜完整性、总畸形率和丙二醛(MDA)生成情况进行了评估:结果表明,添加1 µg/mL的ZnO-NPs和Curc-co-ZnO-NPs能显著降低MDA和总畸变的水平(p 结论:ZnO-NPs和Curc-co-ZnO-NPs能显著降低MDA和总畸变的水平:冻融后,Curc-co-ZnO-NPs(1 µg/mL)似乎提高了精子参数。
{"title":"Comparison of the Effect of Adding Different Levels of Zinc Chloride, Curcumin, Zinc Oxide Nanoparticles (Zano-NPs), Curcumin Loaded on Zano-NPs on Post-Thawing Quality of Ram Semen.","authors":"Fatemeh Omidi, Hadi Hajarian, Hamed Karamishabankareh, Leila Soltani, Mojtaba Dashtizad","doi":"10.1002/vms3.70091","DOIUrl":"10.1002/vms3.70091","url":null,"abstract":"<p><strong>Objective: </strong>This study looked at how different concentrations of curcumin (Curc), zinc chloride (ZnCl<sub>2</sub>), zinc oxide nanoparticles (ZnO-NPs) and Curc loaded on ZnO-NPs (Curc-co-ZnO-NPs) in cryopreservation dilution affected the quality of ram sperm after thawing.</p><p><strong>Methods: </strong>ZnO-NPs were synthesised using Berberis vulgaris leaf aqueous extract. Then, Curc was loaded on the ZnO-NPs that had been synthesised. We used analytical methods to look at the composition, morphology and size of green synthesised ZnO-NPs and Curc-co-ZnO-NPs, including UV-Vis, zeta potential, EDX, DLS, FE-SEM and FT-IR. Using a Tris-base extender containing various concentrations of Curc, ZnCl<sub>2</sub>, ZnO-NPs and Curc-co-ZnO-NPs (0, 1, 10 and 100 µg/mL), semen samples from four rams were combined. Sperm motility, viability, DNA and plasma membrane integrity, total abnormalities and malondialdehyde (MDA) generation were all evaluated in treatment groups after thawing.</p><p><strong>Results: </strong>The results showed that adding 1 µg/mL of ZnO-NPs and Curc-co-ZnO-NPs significantly reduced the level of MDA and total abnormalities (p < 0.05). Additionally, following the freeze-thawing procedure, the presence of 1 µg/mL of Curc-co-ZnO-NPs in the diluent of ram sperm significantly increased the percentage of sperm viability and motility in comparison to the control and other treatment groups (p < 0.05). Furthermore, as compared to the control group and other treatments, treatments containing 1 µg/mL of Curc-co-ZnO-NPs significantly improved membrane and DNA integrity (p < 0.05).</p><p><strong>Conclusions: </strong>It appears that following freeze-thawing, the Curc-co-ZnO-NPs (1 µg/mL) enhanced sperm parameters.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11533303/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142569719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Veterinary Medicine and Science
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1