Over-expression of the Iris laevigata cold-resistance gene MYB97 improves photosynthetic capacity and photoprotection in tobacco (Nicotiana tabacum)

IF 3.4 2区 农林科学 Q1 FORESTRY Journal of Forestry Research Pub Date : 2024-06-25 DOI:10.1007/s11676-024-01752-4
Yu Shu, Ruiyang Zhao, Nuo Xu, Yingxuan Dai, Jyoti R. Bhera, Aruna Kilaru, Ling Wang
{"title":"Over-expression of the Iris laevigata cold-resistance gene MYB97 improves photosynthetic capacity and photoprotection in tobacco (Nicotiana tabacum)","authors":"Yu Shu, Ruiyang Zhao, Nuo Xu, Yingxuan Dai, Jyoti R. Bhera, Aruna Kilaru, Ling Wang","doi":"10.1007/s11676-024-01752-4","DOIUrl":null,"url":null,"abstract":"<p>In northern China, light and temperature are major limiting factors for plant growth, particularly during seed production and seedling establishment. While previous studies suggested a possible role for the <i>MYB97</i> gene in cold-stress, confirmation through documented evidence was lacking. In this study, we transformed the <i>MYB97</i> gene from <i>Iris laevigata</i> into tobacco, and discovered that the gene boosted photosynthesis, photoprotection and resilience to cold. The transgenic tobacco seeds exhibited enhanced germination and accelerated seedling growth. Moreover, these plants had decreased levels of MDA (Malondialdehyde) and relative conductance, coupled with elevated concentrations of proline and soluble sugars. This response was accompanied by heightened activity of antioxidant enzymes during periods of cold stress (4 and − 2 °C). Exposure to low temperatures (0–15 °C) also reduced heights but accentuated primary root growth in transgenic tobacco plants. Additionally, tobacco leaves showed an increased growth along with higher chlorophyll levels, net photosynthetic rates, stomatal conductance, transpiration rates and non-photochemical quenching coefficient. This study shows that <i>IlMYB97</i> (The <i>MYB97</i> genes in <i>I. laevigata</i>) improves cold-resistance, and enhances photosynthesis and photoprotective ability, and thus overall growth and development. These findings would offer the genetic resources to further study cold resistance and photosynthesis.</p>","PeriodicalId":15830,"journal":{"name":"Journal of Forestry Research","volume":"34 1","pages":""},"PeriodicalIF":3.4000,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Forestry Research","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s11676-024-01752-4","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FORESTRY","Score":null,"Total":0}
引用次数: 0

Abstract

In northern China, light and temperature are major limiting factors for plant growth, particularly during seed production and seedling establishment. While previous studies suggested a possible role for the MYB97 gene in cold-stress, confirmation through documented evidence was lacking. In this study, we transformed the MYB97 gene from Iris laevigata into tobacco, and discovered that the gene boosted photosynthesis, photoprotection and resilience to cold. The transgenic tobacco seeds exhibited enhanced germination and accelerated seedling growth. Moreover, these plants had decreased levels of MDA (Malondialdehyde) and relative conductance, coupled with elevated concentrations of proline and soluble sugars. This response was accompanied by heightened activity of antioxidant enzymes during periods of cold stress (4 and − 2 °C). Exposure to low temperatures (0–15 °C) also reduced heights but accentuated primary root growth in transgenic tobacco plants. Additionally, tobacco leaves showed an increased growth along with higher chlorophyll levels, net photosynthetic rates, stomatal conductance, transpiration rates and non-photochemical quenching coefficient. This study shows that IlMYB97 (The MYB97 genes in I. laevigata) improves cold-resistance, and enhances photosynthesis and photoprotective ability, and thus overall growth and development. These findings would offer the genetic resources to further study cold resistance and photosynthesis.

Abstract Image

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
过度表达鸢尾抗寒基因 MYB97 可提高烟草(Nicotiana tabacum)的光合能力和光保护能力
在中国北方,光照和温度是植物生长的主要限制因素,尤其是在种子生产和育苗期间。虽然之前的研究表明 MYB97 基因可能在冷胁迫中发挥作用,但缺乏文献证据证实。在这项研究中,我们将鸢尾的 MYB97 基因转化到烟草中,发现该基因能促进光合作用、光保护和抗寒能力。转基因烟草种子的萌发能力增强,幼苗生长加快。此外,这些植物的 MDA(丙二醛)水平和相对电导率降低,脯氨酸和可溶性糖的浓度升高。在冷胁迫期间(4 °C和- 2 °C),这种反应伴随着抗氧化酶活性的提高。在低温(0-15 °C)条件下,转基因烟草植株的高度也会降低,但主根的生长却会增强。此外,烟草叶片在叶绿素水平、净光合速率、气孔导度、蒸腾速率和非光化学淬灭系数升高的同时,生长速度也有所加快。这项研究表明,IlMYB97(I. laevigata 的 MYB97 基因)能提高抗寒性,增强光合作用和光保护能力,从而促进整体生长和发育。这些发现将为进一步研究抗寒性和光合作用提供遗传资源。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
CiteScore
7.30
自引率
3.30%
发文量
2538
期刊介绍: The Journal of Forestry Research (JFR), founded in 1990, is a peer-reviewed quarterly journal in English. JFR has rapidly emerged as an international journal published by Northeast Forestry University and Ecological Society of China in collaboration with Springer Verlag. The journal publishes scientific articles related to forestry for a broad range of international scientists, forest managers and practitioners.The scope of the journal covers the following five thematic categories and 20 subjects: Basic Science of Forestry, Forest biometrics, Forest soils, Forest hydrology, Tree physiology, Forest biomass, carbon, and bioenergy, Forest biotechnology and molecular biology, Forest Ecology, Forest ecology, Forest ecological services, Restoration ecology, Forest adaptation to climate change, Wildlife ecology and management, Silviculture and Forest Management, Forest genetics and tree breeding, Silviculture, Forest RS, GIS, and modeling, Forest management, Forest Protection, Forest entomology and pathology, Forest fire, Forest resources conservation, Forest health monitoring and assessment, Wood Science and Technology, Wood Science and Technology.
期刊最新文献
Applying palaeoecological analogues to contemporary challenges: community-level effects of canopy gaps caused by systematic decline of a prevalent tree species A stacking-based model for the spread of Botryosphaeria laricina Leaf functional traits and ecological strategies of common plant species in evergreen broad-leaved forests on Huangshan Mountain Characteristics and expression of heat shock gene Lghsp17.4 in Lenzites gibbosa, a white rot fungus of wood Tree diversity drives understory carbon storage rather than overstory carbon storage across forest types
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1