Yu Shu, Ruiyang Zhao, Nuo Xu, Yingxuan Dai, Jyoti R. Bhera, Aruna Kilaru, Ling Wang
{"title":"Over-expression of the Iris laevigata cold-resistance gene MYB97 improves photosynthetic capacity and photoprotection in tobacco (Nicotiana tabacum)","authors":"Yu Shu, Ruiyang Zhao, Nuo Xu, Yingxuan Dai, Jyoti R. Bhera, Aruna Kilaru, Ling Wang","doi":"10.1007/s11676-024-01752-4","DOIUrl":null,"url":null,"abstract":"<p>In northern China, light and temperature are major limiting factors for plant growth, particularly during seed production and seedling establishment. While previous studies suggested a possible role for the <i>MYB97</i> gene in cold-stress, confirmation through documented evidence was lacking. In this study, we transformed the <i>MYB97</i> gene from <i>Iris laevigata</i> into tobacco, and discovered that the gene boosted photosynthesis, photoprotection and resilience to cold. The transgenic tobacco seeds exhibited enhanced germination and accelerated seedling growth. Moreover, these plants had decreased levels of MDA (Malondialdehyde) and relative conductance, coupled with elevated concentrations of proline and soluble sugars. This response was accompanied by heightened activity of antioxidant enzymes during periods of cold stress (4 and − 2 °C). Exposure to low temperatures (0–15 °C) also reduced heights but accentuated primary root growth in transgenic tobacco plants. Additionally, tobacco leaves showed an increased growth along with higher chlorophyll levels, net photosynthetic rates, stomatal conductance, transpiration rates and non-photochemical quenching coefficient. This study shows that <i>IlMYB97</i> (The <i>MYB97</i> genes in <i>I. laevigata</i>) improves cold-resistance, and enhances photosynthesis and photoprotective ability, and thus overall growth and development. These findings would offer the genetic resources to further study cold resistance and photosynthesis.</p>","PeriodicalId":15830,"journal":{"name":"Journal of Forestry Research","volume":"34 1","pages":""},"PeriodicalIF":3.4000,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Forestry Research","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s11676-024-01752-4","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FORESTRY","Score":null,"Total":0}
引用次数: 0
Abstract
In northern China, light and temperature are major limiting factors for plant growth, particularly during seed production and seedling establishment. While previous studies suggested a possible role for the MYB97 gene in cold-stress, confirmation through documented evidence was lacking. In this study, we transformed the MYB97 gene from Iris laevigata into tobacco, and discovered that the gene boosted photosynthesis, photoprotection and resilience to cold. The transgenic tobacco seeds exhibited enhanced germination and accelerated seedling growth. Moreover, these plants had decreased levels of MDA (Malondialdehyde) and relative conductance, coupled with elevated concentrations of proline and soluble sugars. This response was accompanied by heightened activity of antioxidant enzymes during periods of cold stress (4 and − 2 °C). Exposure to low temperatures (0–15 °C) also reduced heights but accentuated primary root growth in transgenic tobacco plants. Additionally, tobacco leaves showed an increased growth along with higher chlorophyll levels, net photosynthetic rates, stomatal conductance, transpiration rates and non-photochemical quenching coefficient. This study shows that IlMYB97 (The MYB97 genes in I. laevigata) improves cold-resistance, and enhances photosynthesis and photoprotective ability, and thus overall growth and development. These findings would offer the genetic resources to further study cold resistance and photosynthesis.
期刊介绍:
The Journal of Forestry Research (JFR), founded in 1990, is a peer-reviewed quarterly journal in English. JFR has rapidly emerged as an international journal published by Northeast Forestry University and Ecological Society of China in collaboration with Springer Verlag. The journal publishes scientific articles related to forestry for a broad range of international scientists, forest managers and practitioners.The scope of the journal covers the following five thematic categories and 20 subjects:
Basic Science of Forestry,
Forest biometrics,
Forest soils,
Forest hydrology,
Tree physiology,
Forest biomass, carbon, and bioenergy,
Forest biotechnology and molecular biology,
Forest Ecology,
Forest ecology,
Forest ecological services,
Restoration ecology,
Forest adaptation to climate change,
Wildlife ecology and management,
Silviculture and Forest Management,
Forest genetics and tree breeding,
Silviculture,
Forest RS, GIS, and modeling,
Forest management,
Forest Protection,
Forest entomology and pathology,
Forest fire,
Forest resources conservation,
Forest health monitoring and assessment,
Wood Science and Technology,
Wood Science and Technology.