Selective induction of senescence in cancer cells through near‐infrared light treatment via mitochondrial modulation

IF 2 3区物理与天体物理 Q3 BIOCHEMICAL RESEARCH METHODS Journal of Biophotonics Pub Date : 2024-06-22 DOI:10.1002/jbio.202400046

I. Kalampouka, R. R. Mould, S. W. Botchway, A. M. Mackenzie, A. V. Nunn, E. L. Thomas, J. D. Bell

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Abstract

Photobiomodulation, utilising non‐ionising light in the visible and near‐infrared (NIR) spectrum, has been suggested as a potential method for enhancing tissue repair, reducing inflammation and possibly mitigating cancer‐therapy‐associated side effects. NIR light is suggested to be absorbed intracellularly, mainly by chromophores within the mitochondria. This study examines the impact of 734 nm NIR light on cellular senescence. Cancer (MCF7 and A549) and non‐cancer (MCF10A and IMR‐90) cell populations were subjected to 63 mJ/cm2 NIR‐light exposure for 6 days. Senescence levels were quantified by measuring active senescence‐associated beta‐galactosidase. Exposure to NIR light significantly increases senescence levels in cancer (10.0%–203.2%) but not in non‐cancer cells (p > 0.05). Changes in senescence were associated with significant modulation of mitochondrial homeostasis, including increased levels of reactive oxygen species (p < 0.05) and mitochondrial membrane potential (p < 0.05) post‐NIR‐light treatment. These results suggest that NIR light modulates cellular chemistry, arresting the proliferation of cancer cells via senescence induction while sparing non‐cancer cells.

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通过线粒体调控近红外光处理选择性诱导癌细胞衰老

利用可见光和近红外（NIR）光谱中的非电离光进行光生物调节，已被认为是一种潜在的方法，可用于加强组织修复、减少炎症反应，并可能减轻癌症治疗相关的副作用。近红外线被认为可在细胞内被吸收，主要是被线粒体内的发色团吸收。本研究探讨了 734 纳米近红外线对细胞衰老的影响。将癌细胞（MCF7 和 A549）和非癌细胞（MCF10A 和 IMR-90）置于 63 mJ/cm2 的近红外光照射下 6 天。通过测量活性衰老相关的 beta-半乳糖苷酶来量化衰老水平。暴露于近红外光会明显增加癌细胞的衰老水平（10.0%-203.2%），但不会增加非癌细胞的衰老水平（p > 0.05）。衰老的变化与线粒体稳态的显著调节有关，包括近红外光处理后活性氧水平（p < 0.05）和线粒体膜电位（p < 0.05）的增加。这些结果表明，近红外光能调节细胞化学反应，通过诱导衰老阻止癌细胞增殖，同时保护非癌细胞。

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来源期刊

Journal of Biophotonics 生物-生化研究方法

CiteScore

5.70

自引率

7.10%

发文量

248

审稿时长

1 months

期刊介绍： The first international journal dedicated to publishing reviews and original articles from this exciting field, the Journal of Biophotonics covers the broad range of research on interactions between light and biological material. The journal offers a platform where the physicist communicates with the biologist and where the clinical practitioner learns about the latest tools for the diagnosis of diseases. As such, the journal is highly interdisciplinary, publishing cutting edge research in the fields of life sciences, medicine, physics, chemistry, and engineering. The coverage extends from fundamental research to specific developments, while also including the latest applications.

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