E. R. Salimgareeva, E. L. Gerasimova, A. V. Karmanova, K. K. Salikova, S. Yu. Saraeva, A. V. Ivanova
{"title":"Chronoamperometric Determination of Antioxidant Capacity Using an Iron Complex with 2,2'-Bipyridine","authors":"E. R. Salimgareeva, E. L. Gerasimova, A. V. Karmanova, K. K. Salikova, S. Yu. Saraeva, A. V. Ivanova","doi":"10.1134/s1061934824700047","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The approach for the determination of antioxidants of various hydrophilicity using the complex of the iron(III) with bipyridine as a model oxidizing agent and chronoamperometric recording of the analytical signal is proposed. The choice of the oxidant is determined by its solubility in aqueous, organic and aqueous-organic media. The conditions for recording chronoamperograms are selected: the medium is the acetonitrile-acetate buffer solution (pH 3.6) (9 : 1), the electrolyte is the LiClO4, the potential is <i>E</i> = 1.25 V, the current registration time is 80 s. Antioxidants soluble in organic and aqueous-organic media are studied: α-tocopherol, quercetin, catechin, and caffeic acid. The analytical ranges are (0.5−4) × 10<sup>–4</sup> M. The antioxidant capacity (<b>AOC</b>) of ethanol extracts from medicinal plant materials is determined. A high correlation is observed between the AOC values obtained by the chronoamperometric and the spectrophotometric approaches, but only for samples whose intrinsic colors do not contribute to the absorbance of the Fe(II)-bipyridine complex. The application of the proposed approach and the potentiometric method using the K<sub>3</sub>[Fe(CN)<sub>6</sub>]/K<sub>4</sub>[Fe(CN)<sub>6</sub>] system showed that the values obtained by the potentiometric approach are significantly lower for most of the studied infusions. Thus, it is advisable to analyze multicomponent objects, containing substances of different hydrophilicity, using approaches with oxidizing agents of different solubilities, such as the Fe(III)-bipyridine complex.</p>","PeriodicalId":606,"journal":{"name":"Journal of Analytical Chemistry","volume":"16 1","pages":""},"PeriodicalIF":1.0000,"publicationDate":"2024-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Analytical Chemistry","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1134/s1061934824700047","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
The approach for the determination of antioxidants of various hydrophilicity using the complex of the iron(III) with bipyridine as a model oxidizing agent and chronoamperometric recording of the analytical signal is proposed. The choice of the oxidant is determined by its solubility in aqueous, organic and aqueous-organic media. The conditions for recording chronoamperograms are selected: the medium is the acetonitrile-acetate buffer solution (pH 3.6) (9 : 1), the electrolyte is the LiClO4, the potential is E = 1.25 V, the current registration time is 80 s. Antioxidants soluble in organic and aqueous-organic media are studied: α-tocopherol, quercetin, catechin, and caffeic acid. The analytical ranges are (0.5−4) × 10–4 M. The antioxidant capacity (AOC) of ethanol extracts from medicinal plant materials is determined. A high correlation is observed between the AOC values obtained by the chronoamperometric and the spectrophotometric approaches, but only for samples whose intrinsic colors do not contribute to the absorbance of the Fe(II)-bipyridine complex. The application of the proposed approach and the potentiometric method using the K3[Fe(CN)6]/K4[Fe(CN)6] system showed that the values obtained by the potentiometric approach are significantly lower for most of the studied infusions. Thus, it is advisable to analyze multicomponent objects, containing substances of different hydrophilicity, using approaches with oxidizing agents of different solubilities, such as the Fe(III)-bipyridine complex.
期刊介绍：
The Journal of Analytical Chemistry is an international peer reviewed journal that covers theoretical and applied aspects of analytical chemistry; it informs the reader about new achievements in analytical methods, instruments and reagents. Ample space is devoted to problems arising in the analysis of vital media such as water and air. Consideration is given to the detection and determination of metal ions, anions, and various organic substances. The journal welcomes manuscripts from all countries in the English or Russian language.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
