Cell-free supernatant-assisted biogenic silver nanoparticles enhance the antibacterial efficacy of communicating bacterial pathogens

IF 2.5 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnology and Bioprocess Engineering Pub Date : 2024-06-21 DOI:10.1007/s12257-024-00122-5
Raghavan Srimathi, Tesalonika Sondak, Kwang-sun Kim
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Abstract

The use of nanoparticles (NPs) as an alternative to the current generation of conventional antibiotics has exploded in the research community in recent years, as evidence of the superiority of NPs over antibiotics in the treatment of pathogens has been steadily presented. However, therapy with NPs may result in the removal of both multidrug-resistant (MDR) pathogens and commensal bacteria due to the broad-spectrum activity of NPs and the non-specificity of target bacteria. Therefore, the fabrication of MDR-pathogen-targeting NPs is necessary. In this study, biogenic silver nanoparticles (Bio-AgNPs) were synthesized using bacterial cell-free supernatant from three communicating gram-negative bacteria. The size, physical features, and morphology of the AgNPs were characterized by dynamic light scattering (an average size of 158–168 nm), X-ray diffraction (co-ordinate patterns), and transmission electron microscopy (spherical structure). The antibacterial activity of the Bio-AgNPs as minimum inhibitory concentration values was obtained between 0.8 and > 6.4 μg mL−1 for bacterial strains. Mechanistic studies of Bio-AgNPs have revealed that biofilm inhibition, protein leakage, hyperproduction of reactive oxygen species, and physical cell damage are plausible mechanisms underlying the activity of Bio-AgNPs against gram-negative pathogens. Overall, the Bio-AgNPs synthesized in this study may bolster the potential use of Bio-AgNPs as a stand-in for traditional antibiotics, and offer potential specificity against bacterial targets.

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无细胞超物质辅助的生物银纳米粒子增强了传播细菌病原体的抗菌功效
近年来,纳米粒子(NPs)作为新一代传统抗生素的替代品在研究界掀起了一股热潮,因为不断有证据表明 NPs 在治疗病原体方面优于抗生素。然而,由于 NPs 的广谱活性和目标细菌的非特异性,使用 NPs 治疗可能会同时清除多重耐药(MDR)病原体和共生细菌。因此,有必要制备 MDR 病原体靶向 NPs。本研究利用三种交流革兰氏阴性菌的细菌无细胞上清液合成了生物银纳米粒子(Bio-AgNPs)。通过动态光散射(平均尺寸为 158-168 纳米)、X 射线衍射(坐标图)和透射电子显微镜(球形结构)对银纳米粒子的尺寸、物理特征和形态进行了表征。Bio-AgNPs 的抗菌活性在 0.8 至 6.4 μg mL-1 之间。对 Bio-AgNPs 的机理研究表明,生物膜抑制、蛋白质渗漏、活性氧生成过多和细胞物理损伤是 Bio-AgNPs 对革兰阴性病原体活性的合理机制。总之,本研究合成的生物氮化镁可能会加强生物氮化镁作为传统抗生素替代品的潜在用途,并提供针对细菌靶标的潜在特异性。
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来源期刊
Biotechnology and Bioprocess Engineering
Biotechnology and Bioprocess Engineering 工程技术-生物工程与应用微生物
CiteScore
5.00
自引率
12.50%
发文量
79
审稿时长
3 months
期刊介绍: Biotechnology and Bioprocess Engineering is an international bimonthly journal published by the Korean Society for Biotechnology and Bioengineering. BBE is devoted to the advancement in science and technology in the wide area of biotechnology, bioengineering, and (bio)medical engineering. This includes but is not limited to applied molecular and cell biology, engineered biocatalysis and biotransformation, metabolic engineering and systems biology, bioseparation and bioprocess engineering, cell culture technology, environmental and food biotechnology, pharmaceutics and biopharmaceutics, biomaterials engineering, nanobiotechnology, and biosensor and bioelectronics.
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