Hydrazine-derived salts: Unveiling their diverse potential through synthesis, characterization, pharmacology, and theoretical analysis

Abstract

This study focuses on newly synthesized compounds derived from hydrazine derivatives, specifically methyl carbazate (MCZ, CHNO) and 2-thiobarbituric acid (2-TBA, CHNOS). The reaction between MCZ and 2-TBA resulted in the formation of three types of salts with the following compositions: [(CHNO)(CHNOS) (CHNO)(CHNOS)(CHNO) ), and (CHNO)(CHN)(CHNOS) [(CHN) represents the triethyl ammonium cation]. The prepared salts were comprehensively characterized, showing that compound exhibited a monoclinic crystal structure and met Lipinski’s rules, indicating significant drug-like properties. Molecular docking analysis revealed that these compounds exhibited strong binding affinities toward bacterial proteins, particularly and , indicating their potential as antibacterial agents. The synthesized compounds demonstrated remarkable free radical-scavenging abilities, as evidenced by DPPH assays. The results of pharmacological evaluations, including brine shrimp lethality assays, antibacterial tests, and anticancer studies on MCF-7 cell lines, revealed that the compounds exhibit highly significant activities, particularly cytotoxic effects, suggesting their potential as anticancer agents. The compound emerged as the most effective anticancer agent against MCF-7 cells. These findings underscore the broad potential of these compounds, especially , as promising agents with significant antibacterial, antioxidant, and anticancer activities, suggesting that they may contribute to the field of medicinal chemistry with implications for therapeutic applications.