E. D. Khilazheva, Yu. A. Panina, A. I. Mosiagina, O. S. Belozor, Yu. K. Komleva
{"title":"Glibenclamide Prevents Inflammation by Targeting NLRP3 Inflammasome Activation in vitro","authors":"E. D. Khilazheva, Yu. A. Panina, A. I. Mosiagina, O. S. Belozor, Yu. K. Komleva","doi":"10.1134/s002209302403013x","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The NLRP3 inflammasome plays a significant role in the development\nof neurodegeneration, physiological aging and metabolic inflammation,\nwhich actualizes the search for effective NLRP3 inflammasome inhibitors\nand assessing their effects. This study was aimed to assess the\neffect of pharmacological modulation of NLRP3 activity by an indirect\nNLRP3 inflammasome inhibitor, glibenclamide, on the in vitro expression\nof metaflammasome components in brain cells derived from middle-aged\nmice. It was found that glibenclamide reduces the expression of\npro-inflammatory markers NLRP3 and IL18 in cell culture, which in\nturn prevents the phosphorylation of metaflammasome complex protein\nkinases, PKR and IKKβ. At the same time, there were no changes in\nthe expression of pathologically phosphorylated IRS, as well as\nin the number of senescent cells in cultures after glibenclamide\nexposure.</p>","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"4 1","pages":""},"PeriodicalIF":0.5000,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Evolutionary Biochemistry and Physiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1134/s002209302403013x","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The NLRP3 inflammasome plays a significant role in the development
of neurodegeneration, physiological aging and metabolic inflammation,
which actualizes the search for effective NLRP3 inflammasome inhibitors
and assessing their effects. This study was aimed to assess the
effect of pharmacological modulation of NLRP3 activity by an indirect
NLRP3 inflammasome inhibitor, glibenclamide, on the in vitro expression
of metaflammasome components in brain cells derived from middle-aged
mice. It was found that glibenclamide reduces the expression of
pro-inflammatory markers NLRP3 and IL18 in cell culture, which in
turn prevents the phosphorylation of metaflammasome complex protein
kinases, PKR and IKKβ. At the same time, there were no changes in
the expression of pathologically phosphorylated IRS, as well as
in the number of senescent cells in cultures after glibenclamide
exposure.
期刊介绍：
Journal of Evolutionary Biochemistry and Physiology publishes original experimental and theoretical and review articles related to evolution of the main forms of metabolism in connection with life origin; comparative and ontogenetic physiology and biochemistry, biochemical evolution of animal world; as well as evolution of functions; morphology, pharmacology, pathophysiology and ecological physiology. The journal welcomes manuscripts from all countries in the English or Russian language.
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