Reversible, stable and uniform SERS in a Y-shaped microfluidic chip: chemical imaging of concentration gradients

IF 2.3 4区 工程技术 Q2 INSTRUMENTS & INSTRUMENTATION Microfluidics and Nanofluidics Pub Date : 2024-07-03 DOI:10.1007/s10404-024-02740-0
Fabien Chauvet
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Abstract

Imaging of chemical composition in microfluidic chips is addressed using Surface Enhanced Raman Spectroscopy (SERS). The Y-shaped SERS microfluidic chip used is fabricated by xurography and an electrodeposition method is employed to form a thin nanostructured silver layer over the bottom glass wall of the main microchannel. Used as an immobilized SERS substrate, this layer of silver nanocrystals exhibits an analytical enhancement factor of 5.10\(^4\) uniformly distributed over its surface (RSD < 7%). These good performances allow the quantitative imaging of transverse diffusion profiles of Crystal Violet (CV) at low concentrations (\(10^{-8}-10^{-6}\) mol/L). The SERS measurement turns out to be reversible at high laser power and this is explained by the thermal desorption of adsorbed CV (photothermal effect). However, too high heating leads to a low amount of adsorbed species and a low SERS signal. This effect is limited by using a fast enough flow inducing a cooling effect. A compromise must be found between laser power and liquid flow rate to enable a reversible and sensitive SERS measurement in the chip. These findings should contribute to the development of imaging, in microfluidic conditions, of the spatiotemporal dynamics of weakly concentrated key molecules involved in chemical, biochemical or biological processes.

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Y 形微流体芯片中可逆、稳定和均匀的 SERS:浓度梯度的化学成像
利用表面增强拉曼光谱(SERS)技术对微流控芯片中的化学成分进行成像。所使用的 Y 型 SERS 微流控芯片是通过 Xurography 技术制造的,并采用电沉积方法在主微通道的底部玻璃壁上形成了一层薄薄的纳米结构银层。作为固定的 SERS 基底,这层纳米银晶体在其表面均匀分布,显示出 5.10\(^4\) 的分析增强因子(RSD <7%)。这些良好的性能允许在低浓度(\(10^{-8}-10^{-6}\) mol/L)下对水晶紫(CV)的横向扩散曲线进行定量成像。在高激光功率下,SERS 测量结果是可逆的,这可以用吸附的 CV 的热解吸(光热效应)来解释。然而,过高的加热会导致吸附物种的数量减少和 SERS 信号降低。通过使用足够快的流速来产生冷却效应,可以限制这种效应。必须在激光功率和液体流速之间找到一个折衷方案,才能在芯片中进行可逆和灵敏的 SERS 测量。这些发现将有助于在微流控条件下对化学、生化或生物过程中涉及的弱浓缩关键分子的时空动态进行成像。
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来源期刊
Microfluidics and Nanofluidics
Microfluidics and Nanofluidics 工程技术-纳米科技
CiteScore
4.80
自引率
3.60%
发文量
97
审稿时长
2 months
期刊介绍: Microfluidics and Nanofluidics is an international peer-reviewed journal that aims to publish papers in all aspects of microfluidics, nanofluidics and lab-on-a-chip science and technology. The objectives of the journal are to (1) provide an overview of the current state of the research and development in microfluidics, nanofluidics and lab-on-a-chip devices, (2) improve the fundamental understanding of microfluidic and nanofluidic phenomena, and (3) discuss applications of microfluidics, nanofluidics and lab-on-a-chip devices. Topics covered in this journal include: 1.000 Fundamental principles of micro- and nanoscale phenomena like, flow, mass transport and reactions 3.000 Theoretical models and numerical simulation with experimental and/or analytical proof 4.000 Novel measurement & characterization technologies 5.000 Devices (actuators and sensors) 6.000 New unit-operations for dedicated microfluidic platforms 7.000 Lab-on-a-Chip applications 8.000 Microfabrication technologies and materials Please note, Microfluidics and Nanofluidics does not publish manuscripts studying pure microscale heat transfer since there are many journals that cover this field of research (Journal of Heat Transfer, Journal of Heat and Mass Transfer, Journal of Heat and Fluid Flow, etc.).
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