Exogenous ethephon treatment on the biosynthesis and accumulation of astragaloside IV in Astragalus membranaceus Bge. Var. Mongholicus (Bge.) Hsiao.

IF 3.4 3区生物学 Q1 Agricultural and Biological Sciences Botanical Studies Pub Date : 2024-07-05 DOI:10.1186/s40529-024-00426-y

Haonan Wu, Hang Cui, Yu Tian, Jiawen Wu, Zhenqing Bai, Xiujuan Zhang

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Abstract

Background: Astragaloside IV is a main medicinal active ingredient in Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao, which is also the key biomarker of A. membranaceus quality. Ethylene has been well-documented to involve in secondary metabolites biosynthesis in plants. Nevertheless, how ethylene regulates astragaloside IV biosynthesis in A. membranaceus is still unclear. Therefore, in the present study different dosages and time-dependent exogenous application of ethephon (Eth) were employed to analyze astragaloside IV accumulation and its biosynthesis genes expression level in hydroponically A. membranaceus.

Results: Exogenous 200 µmol·L^- 1Eth supply is most significantly increased astragaloside IV contents in A. membranaceus when compared with non-Eth supply. After 12 h 200 µmol·L^- 1 Eth treatment, the astragaloside IV contents reaching the highest content at 3 d Eth treatment(P ≤ 0.05). Moreover, After Eth treatment, all detected key genes involved in astragaloside IV synthesis were significant decrease at 3rd day(P ≤ 0.05). However, SE displayed a significant increase at the 3rd day under Eth treatment(P ≤ 0.05). Under Eth treatment, the expression level of FPS, HMGR, IDI, SS, and CYP93E3 exhibited significant negative correlations with astragaloside IV content, while expression level of SE displayed a significant positive correlation.

Conclusions: These findings suggest that exogenous Eth treatment can influence the synthesis of astragaloside IV by regulating the expression of FPS, HMGR, IDI, SS, CYP93E3 and SE. This study provides a theoretical basis for utilizing molecular strategies to enhance the quality of A. membranaceus.

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外源乙硫磷处理对膜质黄芪中黄芪皂苷 IV 的生物合成和积累的影响Var.Mongholicus (Bge.) Hsiao.

研究背景黄芪皂苷 IV 是膜荚黄芪的主要药用活性成分，也是衡量膜荚黄芪品质的关键生物标志物。乙烯参与植物次生代谢物的生物合成已被充分证明。然而，乙烯如何调控膜衣草中黄芪皂苷 IV 的生物合成仍不清楚。因此，本研究采用了不同剂量和时间依赖性的外源乙烯利（Eth），以分析水培膜柄菊中黄芪皂苷 IV 的积累及其生物合成基因的表达水平：结果：外源 200 µmol-L- 1Eth 与不外源相比，最显著地增加了膜葡萄中黄芪皂苷 IV 的含量。经过 12 h 200 µmol-L- 1 Eth 处理后，黄芪皂苷 IV 的含量在 Eth 处理 3 d 时达到最高（P ≤ 0.05）。此外，乙醇处理后，所有检测到的参与黄芪皂苷 IV 合成的关键基因在第 3 天均显著减少（P ≤ 0.05）。然而，SE 在乙醇处理的第 3 天明显增加（P ≤ 0.05）。在 Eth 处理条件下，FPS、HMGR、IDI、SS 和 CYP93E3 的表达水平与黄芪皂苷 IV 的含量呈显著负相关，而 SE 的表达水平则呈显著正相关：这些研究结果表明，外源 Eth 处理可通过调节 FPS、HMGR、IDI、SS、CYP93E3 和 SE 的表达来影响黄芪皂苷 IV 的合成。这项研究为利用分子策略提高膜荚果的品质提供了理论依据。

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来源期刊

Botanical Studies 生物-植物科学

CiteScore

5.50

自引率

2.90%

发文量

审稿时长

2.4 months

期刊介绍： Botanical Studies is an open access journal that encompasses all aspects of botany, including but not limited to taxonomy, morphology, development, genetics, evolution, reproduction, systematics, and biodiversity of all plant groups, algae, and fungi. The journal is affiliated with the Institute of Plant and Microbial Biology, Academia Sinica, Taiwan.