Comparative study of antifungal susceptibility testing methods for clinical Candida albicans isolates

IF 1.4 4区医学 Q4 IMMUNOLOGY Indian Journal of Medical Microbiology Pub Date : 2024-07-01 DOI:10.1016/j.ijmmb.2024.100663

Xue Ting Tan, Nurliyana binti Mohd Shuhairi, Ratna Mohd Tap, Fairuz Amran

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Abstract

Purpose

Candida albicans is the second most common cause of candidemia in Malaysia. The Clinical and Laboratory Standards Institute (CLSI) broth microdilution method is the gold standard for determining its minimum inhibitory concentration (MIC); however, it is laborious and time-consuming. This study was conducted to evaluate the usefulness of alternative methods, namely Sensititre YeastOne (SYO), VITEK 2 system, and E-test for determining the MIC of clinical C. albicans isolates.

Materials and methods

The susceptibilities of 95 C. albicans isolates were compared between SYO, VITEK 2 system, and E-test with CLSI broth microdilution method. The categorical agreement (CA), essential agreement (EA), very major errors (VME), major errors (ME) and minor errors (MiE) were calculated.

Results

Our finding showed the CA varied for SYO from 96.8% to 100%, while the EA ranged from 91.6% to 100%. The SYO method showed 1.1% of VME and ME, and up to 3.2% of MiE. Next, the CA and EA ranges for the VITEK 2 system were 97.8%–100% and 23.2%–100%, respectively. In the VITEK 2 technique, 1.1% of VME were found. For the E-test, the CA varied from 83.2% to 100% while the EA ranged from 64.2% to 98.9%. The E-test method showed 1.1% of VME and up to 16.8% of MiE.

Conclusions

In conclusion, SYO and VITEK 2 (except flucytosine) could be potential alternatives to the CLSI broth microdilution method in determining the MIC of C. albicans.

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临床白色念珠菌分离物抗真菌药敏试验方法比较研究

目的：白色念珠菌是马来西亚第二大常见的念珠菌血症病因。临床和实验室标准协会（CLSI）的肉汤微量稀释法是确定其最低抑菌浓度（MIC）的黄金标准，但该方法费时费力。本研究旨在评估 Sensititre YeastOne (SYO)、VITEK 2 系统和 E-test 等替代方法在确定临床白僵菌分离物 MIC 方面的实用性：比较了 SYO、VITEK 2 系统和 E-test 与 CLSI 肉汤微量稀释法对 95 个白僵菌分离株的药敏性。计算了分类一致性（CA）、基本一致性（EA）、非常大误差（VME）、大误差（ME）和小误差（MiE）：结果表明，SYO 法的 CA 值从 96.8% 到 100% 不等，而 EA 值从 91.6% 到 100% 不等。SYO 方法的 VME 和 ME 为 1.1%，MiE 高达 3.2%。接下来，VITEK 2 系统的 CA 和 EA 范围分别为 97.8% 至 100% 和 23.2% 至 100% 。在 VITEK 2 技术中，发现了 1.1%的 VME。在 E 测试中，CA 的变化范围为 83.2% 至 100%，而 EA 的变化范围为 64.2% 至 98.9%。E 测试法显示出 1.1% 的 VME 和高达 16.8% 的 MiE：总之，SYO 和 VITEK 2（氟胞嘧啶除外）可以替代 CLSI 肉汤微量稀释法来确定白僵菌的 MIC。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Indian Journal of Medical Microbiology IMMUNOLOGY-

CiteScore

2.20

自引率

0.00%

发文量

154

审稿时长

73 days

期刊介绍： Manuscripts of high standard in the form of original research, multicentric studies, meta analysis, are accepted. Current reports can be submitted as brief communications. Case reports must include review of current literature, clinical details, outcome and follow up. Letters to the editor must be a comment on or pertain to a manuscript already published in the IJMM or in relation to preliminary communication of a larger study. Review articles, Special Articles or Guest Editorials are accepted on invitation.