Molecular Epidemiology of Human Parainfluenza Virus Type 3 in Children With Acute Respiratory Tract Infection in Hangzhou

IF 4.3 4区医学 Q1 INFECTIOUS DISEASES Influenza and Other Respiratory Viruses Pub Date : 2024-07-04 DOI:10.1111/irv.13351

Ya-jun Guo, Lin Li, Qin-rui Lai, Ying-shuo Wang, Wei Li

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Abstract

Background

Since the outbreak of COVID-19, China has undertaken a variety of preventative and control measures, effectively reducing the incidence of numerous infectious diseases among the pediatric population in Hangzhou. We aim to investigate the genetic and epidemiological characteristics of Human parainfluenza virus-3 (HPIV-3) in pediatric patients during this period.

Methods

A total of 1442 pharyngeal swab samples were collected from outpatients and inpatients with a diagnosis of acute respiratory tract infections (ARTIs) from November 2020 to March 2021. HPIV-3 was detected by quantitative real time polymerase chain reaction (qRT-PCR). The L gene of HPIV-3 positive samples was amplified and sequenced.

Results

Among 1442 children with ARTI, the positive rate of HPIV-3 was 7.07% (102/1442). The positive detection rate was the highest in the 6-month to 1-year age group. Coinfection was observed in 36 HPIV-3-positive samples (35.29%, 36/102), and adenovirus (ADV) was the most common coinfecting virus (63.89%, 23/36). The L gene of 48 HPIV-3 positive samples was sequenced. The nucleotide sequence analysis showed high consistency (92.10%–99.40%), and all strains belonged to C3a.

Conclusions

During study periods, the positive detection rate of HPIV-3 among children is high, and the highest proportion of coinfection was observed in HPIV-3 mixed ADV infection. Phylogenetic analysis revealed that the nucleotide sequence of the L gene of HPIV-3 was highly consistent, and the main epidemic strain in this area was the C3a subtype.

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杭州急性呼吸道感染儿童中人副流感病毒 3 型的分子流行病学研究

背景：自COVID-19疫情爆发以来，我国采取了多种防控措施，有效降低了杭州市儿科人群中多种传染病的发病率。我们旨在调查这一时期儿科患者中人副流感病毒-3（HPIV-3）的遗传学和流行病学特征：方法：从 2020 年 11 月至 2021 年 3 月，我们从诊断为急性呼吸道感染（ARTI）的门诊和住院患者中采集了 1442 份咽拭子样本。通过定量实时聚合酶链反应（qRT-PCR）检测 HPIV-3。对HPIV-3阳性样本的L基因进行扩增和测序：结果：在1442名ARTI患儿中，HPIV-3阳性率为7.07%（102/1442）。6 个月至 1 岁年龄组的阳性检出率最高。在 36 份 HPIV-3 阳性样本（35.29%，36/102）中观察到合并感染，腺病毒（ADV）是最常见的合并感染病毒（63.89%，23/36）。对 48 份 HPIV-3 阳性样本的 L 基因进行了测序。核苷酸序列分析表明，所有菌株均属于C3a，一致性较高（92.10%-99.40%）：结论：在研究期间，儿童 HPIV-3 阳性检出率较高，HPIV-3 混合 ADV 感染中合并感染的比例最高。系统发育分析表明，HPIV-3的L基因核苷酸序列高度一致，该地区的主要流行株为C3a亚型。

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来源期刊

Influenza and Other Respiratory Viruses 医学-病毒学

CiteScore

7.20

自引率

4.50%

发文量

120

审稿时长

6-12 weeks

期刊介绍： Influenza and Other Respiratory Viruses is the official journal of the International Society of Influenza and Other Respiratory Virus Diseases - an independent scientific professional society - dedicated to promoting the prevention, detection, treatment, and control of influenza and other respiratory virus diseases. Influenza and Other Respiratory Viruses is an Open Access journal. Copyright on any research article published by Influenza and Other Respiratory Viruses is retained by the author(s). Authors grant Wiley a license to publish the article and identify itself as the original publisher. Authors also grant any third party the right to use the article freely as long as its integrity is maintained and its original authors, citation details and publisher are identified.