Modulation in the ratio of abscisic acid to gibberellin level determines genetic variation of seed dormancy in barley (Hordeum vulgare L.)

IF 4 3区生物学 Q1 PLANT SCIENCES Journal of plant physiology Pub Date : 2024-06-28 DOI:10.1016/j.jplph.2024.154301

Parneet K. Toora , Pham Anh Tuan , Tran-Nguyen Nguyen , Ana Badea , Belay T. Ayele

{"title":"Modulation in the ratio of abscisic acid to gibberellin level determines genetic variation of seed dormancy in barley (Hordeum vulgare L.)","authors":"Parneet K. Toora , Pham Anh Tuan , Tran-Nguyen Nguyen , Ana Badea , Belay T. Ayele","doi":"10.1016/j.jplph.2024.154301","DOIUrl":null,"url":null,"abstract":"<div>Abscisic acid (ABA) and gibberellin (GA) are major regulators of seed dormancy, an adaptive trait closely associated with preharvest sprouting. This study examined transcriptional regulation of ABA and GA metabolism genes and modulation of ABA and GA levels in seeds of barley genotypes exhibiting a range of dormancy phenotype. We observed a very strong negative correlation between genetic variation in seed germination and embryonic ABA level (r = 0.85), which is regulated by transcriptional modulation of HvNCED1 and/or HvCYP707A genes. A strong positive correlation was evident between variation in seed germination and GA level (r = 0.64), mediated via transcriptional regulation of GA biosynthesis genes, HvGA20ox2 and/or HvGA3oxs, and GA catabolism genes, HvGA2ox3 and/or HvGA3ox6. Modulation of the ABA and GA levels in the genotypes led to the prevalence of ABA to GA level ratio that exhibited a very strong negative correlation (r = 0.84) with seed germination, highlighting the importance of a shift in ABA/GA ratio in determining genetic variation of dormancy in barley seeds. Our results overall show that transcriptional regulation of specific ABA and GA metabolism genes underlies genetic variation in ABA/GA ratio and seed dormancy, reflecting the potential use of these genes as molecular tools for enhancing preharvest sprouting resistance in barley.</div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"301 ","pages":"Article 154301"},"PeriodicalIF":4.0000,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0176161724001329/pdfft?md5=4ac036dfc62a154b4144afc461524027&pid=1-s2.0-S0176161724001329-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of plant physiology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0176161724001329","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Abscisic acid (ABA) and gibberellin (GA) are major regulators of seed dormancy, an adaptive trait closely associated with preharvest sprouting. This study examined transcriptional regulation of ABA and GA metabolism genes and modulation of ABA and GA levels in seeds of barley genotypes exhibiting a range of dormancy phenotype. We observed a very strong negative correlation between genetic variation in seed germination and embryonic ABA level (r = 0.85), which is regulated by transcriptional modulation of HvNCED1 and/or HvCYP707A genes. A strong positive correlation was evident between variation in seed germination and GA level (r = 0.64), mediated via transcriptional regulation of GA biosynthesis genes, HvGA20ox2 and/or HvGA3oxs, and GA catabolism genes, HvGA2ox3 and/or HvGA3ox6. Modulation of the ABA and GA levels in the genotypes led to the prevalence of ABA to GA level ratio that exhibited a very strong negative correlation (r = 0.84) with seed germination, highlighting the importance of a shift in ABA/GA ratio in determining genetic variation of dormancy in barley seeds. Our results overall show that transcriptional regulation of specific ABA and GA metabolism genes underlies genetic variation in ABA/GA ratio and seed dormancy, reflecting the potential use of these genes as molecular tools for enhancing preharvest sprouting resistance in barley.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

脱落酸与赤霉素水平比例的调节决定了大麦（Hordeum vulgare L.）种子休眠的遗传变异。

脱落酸（ABA）和赤霉素（GA）是种子休眠的主要调节因子，种子休眠是一种与收获前萌发密切相关的适应性性状。本研究考察了表现出一系列休眠表型的大麦基因型种子中 ABA 和 GA 代谢基因的转录调控以及 ABA 和 GA 水平的调节。我们观察到种子萌发的遗传变异与胚胎 ABA 水平之间存在很强的负相关（r = 0.85），这是由 HvNCED1 和/或 HvCYP707A 基因的转录调控决定的。种子萌发的变化与 GA 水平（r = 0.64）之间明显存在很强的正相关性，这是由 GA 生物合成基因 HvGA20ox2 和/或 HvGA3oxs 以及 GA 分解基因 HvGA2ox3 和/或 HvGA3ox6 的转录调控介导的。对基因型中 ABA 和 GA 水平的调节导致 ABA 与 GA 水平比值的增加，而这一比值与种子萌发呈极强的负相关（r = 0.84），这突出表明了 ABA/GA 比值的变化在决定大麦种子休眠遗传变异中的重要性。我们的研究结果总体上表明，特定 ABA 和 GA 代谢基因的转录调控是 ABA/GA 比值和种子休眠遗传变异的基础，反映了这些基因可能被用作提高大麦收获前抗萌发性的分子工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Journal of plant physiology 生物-植物科学

CiteScore

7.20

自引率

4.70%

发文量

196

审稿时长

32 days

期刊介绍： The Journal of Plant Physiology is a broad-spectrum journal that welcomes high-quality submissions in all major areas of plant physiology, including plant biochemistry, functional biotechnology, computational and synthetic plant biology, growth and development, photosynthesis and respiration, transport and translocation, plant-microbe interactions, biotic and abiotic stress. Studies are welcome at all levels of integration ranging from molecules and cells to organisms and their environments and are expected to use state-of-the-art methodologies. Pure gene expression studies are not within the focus of our journal. To be considered for publication, papers must significantly contribute to the mechanistic understanding of physiological processes, and not be merely descriptive, or confirmatory of previous results. We encourage the submission of papers that explore the physiology of non-model as well as accepted model species and those that bridge basic and applied research. For instance, studies on agricultural plants that show new physiological mechanisms to improve agricultural efficiency are welcome. Studies performed under uncontrolled situations (e.g. field conditions) not providing mechanistic insight will not be considered for publication. The Journal of Plant Physiology publishes several types of articles: Original Research Articles, Reviews, Perspectives Articles, and Short Communications. Reviews and Perspectives will be solicited by the Editors; unsolicited reviews are also welcome but only from authors with a strong track record in the field of the review. Original research papers comprise the majority of published contributions.