Prevalence of β-lactamase genes and integrons among multidrug-resistant Escherichia coli and Salmonella spp recovered from food samples in Ouagadougou, Burkina Faso

IF 2.7 Q2 MULTIDISCIPLINARY SCIENCES Scientific African Pub Date : 2024-07-02 DOI:10.1016/j.sciaf.2024.e02302

Adama Patrice Soubeiga , Dissinviel Stéphane Kpoda , Oumar Traoré , Cheikna Zongo , Aly Savadogo

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Abstract

The resistance of Gram-negative bacteria to β-lactam antibiotics occurs mainly by the production of β-lactamases. The aim of this work was to evaluate the presence of β-lactamase genes and integrons in multidrug-resistant (MDR) isolates recovered from food. Susceptibility to antibiotics was determined using the agar diffusion method. Detection of β-lactam resistance genes and integrons was performed with the 53 confirmed MDR strains (42 E. coli and 11 Salmonella spp.) by PCR. High resistance rates of 92.5 %, 60.4 %, and 54.7 % against ampicillin, tetracycline, and amoxicillin/clavulanic acid were observed, respectively. Two β-lactamase genes bla_TEM (37.7 %) and bla_SHV (28.3) were detected among E. coli and Salmonella isolates. High rate of isolates (85 %) exhibited class 1 integrons was observed. These isolates can act as sources of antimicrobial resistance genes and promote the spread of these resistance determinants to other commensal and pathogenic bacteria. Therefore, preventive measures and clean environments are recommended to prevent food contamination with resistant microorganisms.

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布基纳法索瓦加杜古食品样本中发现的耐多药大肠埃希菌和沙门氏菌中的β-内酰胺酶基因和整合子的流行情况

革兰氏阴性细菌对β-内酰胺类抗生素的耐药性主要是通过产生β-内酰胺酶产生的。这项工作的目的是评估从食品中分离出的耐多药（MDR）细菌中是否存在β-内酰胺酶基因和整合子。采用琼脂扩散法测定了对抗生素的敏感性。对 53 株确诊的 MDR 菌株（42 株大肠杆菌和 11 株沙门氏菌）采用 PCR 方法检测其抗 β-内酰胺酶基因和整合子。对氨苄西林、四环素和阿莫西林/克拉维酸的耐药率分别高达 92.5%、60.4% 和 54.7%。在大肠杆菌和沙门氏菌分离物中检测到两种β-内酰胺酶基因 blaTEM（37.7%）和 blaSHV（28.3%）。观察到高分离率（85%）的分离菌表现出 1 类整合子。这些分离物可作为抗菌药耐药性基因的来源，并促进这些耐药性决定簇向其他共生菌和致病菌传播。因此，建议采取预防措施并保持环境清洁，以防止耐药性微生物污染食物。

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