Wei Shi , Si-Qi Zhang , Kai-Bin Li , Xiao-Bin Zhang , Chao-Ying Fang , Tian-Yi Zhang , Deman Han
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引用次数: 0
Abstract
Traditional chromatography often faces issues such as high cost and insufficient selectivity, driving a continuous demand for improved technologies. Current chromatography primarily relies on single-ligand modes, and the methods for its performance optimization are gradually encountering limitations. This study validated the efficacy of integrating a second ligand into the system to boost chromatographic performance, focusing on hydrophobic charge-induction chromatography. Initially, optimal resin (4FF-MMI, 109 µmol/g resin) was targeted, with various densities of the second ligand (4FF-MMI+) assessed for performance enhancement, achieving optimal values at (110+11) µmol/g resin. Characterization, including Fourier Transform Infrared Spectroscopy, Scanning Electron Microscopy and Brunauer-Emmett-Teller, was conducted. Subsequently, Optimization effects under different pH, salt, and flow rate conditions were studied. Results showcase significant chromatographic enhancement with the second ligand. In the final phase, both 4FF-MMI and 4FF-MMI+ were utilized for human IgG separation from human serum, employing pH 8.0 and pH 7.0 as the loading pH, pH 5.0 as the elution pH, and a flow rate of 0.75 mL/min. Compared to 4FF-MMI, 4FF-MMI+ achieved 96.6 % purity and 91.6 % recovery, representing increases of 10.6 % and 20.4 %, respectively. Additionally, the resin with the second ligand exhibits commendable repeatability and stability. In conclusion, this study highlights the potential of adding a second ligand to improve chromatographic capabilities, offering a novel strategy to augment existing ligand libraries.
期刊介绍:
The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology.
The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields:
Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics
Biosensors and Biodevices including biofabrication and novel fuel cell development
Bioseparations including scale-up and protein refolding/renaturation
Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells
Bioreactor Systems including characterization, optimization and scale-up
Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization
Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals
Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release
Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites
Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation
Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis
Protein Engineering including enzyme engineering and directed evolution.